Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R2	775	327.3	10718052	97.2%	10417946	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	97.9%	430.1 / 1.3	93	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (2/0); new base . (50/41); total (52/41)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	96.7%	405.2 / 9.6	93	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (3/0); new base . (47/41); total (52/41)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728663‑3728933

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cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:2459003/140‑1 (MQ=255)

cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:5305578/140‑1 (MQ=255)

 tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccc                                                                                                                                    <  2:1981271/140‑1 (MQ=255)

   aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccccc                                                                                                                                  <  2:2990302/140‑1 (MQ=255)

      cccGGCATAACGCTGCGCAGCTGGTAGATGCGACTGTGCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTCGCCCCACAGccccccccg                                                                                                                               <  1:4116107/138‑2 (MQ=255)

      aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:4116540/140‑2 (MQ=255)

        cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  2:1091839/140‑4 (MQ=255)

                   tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCTCCCCCGTACGGTAATCGAg                                                                                                                >  1:2698980/1‑140 (MQ=255)

                   tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                >  1:2698963/1‑140 (MQ=255)

                   tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                >  1:2699360/1‑140 (MQ=255)

                   tGCGCATCTGGTAGATGCGACCGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCGCTGGATTCGCCGCGATCTTCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑‑CCCCCCCCGTGCGGTAATCGAg                                                                                                                >  2:3968171/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTCCGGTAATCGAgct                                                                                                              >  2:3342946/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                              >  2:4480965/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgct                                                                                                              >  2:3130843/1‑140 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgcc                                                                                                              >  2:726038/1‑139 (MQ=255)

                     cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACCGTAATAGAgct                                                                                                              >  2:4480911/1‑140 (MQ=255)

                        aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCGAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  2:485570/140‑1 (MQ=255)

                          cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         >  1:3589617/1‑140 (MQ=255)

                          cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGCACCGTAATCGAGCTGGTGa                                                                                                         >  1:5113475/1‑140 (MQ=255)

                              tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCAGCGATCTGTGCCAGCCTTGCTGCGCCAAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                     <  2:5132136/140‑1 (MQ=255)

                                  tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCG‑ACGGTGATCGAGCTGCTGACCGCTGat                                                                                                 >  2:1981363/1‑140 (MQ=255)

                                    cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                               >  1:724775/1‑140 (MQ=255)

                                       cTGTTCTAACGGTAATTGAGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCACCGTCAGGTAATCGAGCTGCTGACCGCTGacaaagg                                                                                            >  2:679447/1‑134 (MQ=255)

                                        tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCGCTCCCCGTACGGGGATCGAGCTGGCGACCTCTGGTATCCGc                                                                                           >  1:989434/1‑140 (MQ=255)

                                         gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                          <  1:2047193/140‑1 (MQ=255)

                                         gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                          <  1:2046321/140‑1 (MQ=255)

                                          ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATAGAGCTGCTGACCGGGTATATCCGCCa                                                                                         >  2:764129/1‑140 (MQ=255)

                                           tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  2:2772226/140‑1 (MQ=255)

                                           tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑ACCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATAACCGCCAg                                                                                        >  2:3560777/1‑140 (MQ=255)

                                              aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                     <  2:166608/140‑1 (MQ=255)

                                               aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGCTATTCGCCAGCATc                                                                                    >  1:5303581/1‑140 (MQ=255)

                                               aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCCAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                    >  1:3413812/1‑140 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCTCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:2698980/140‑1 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:2699360/140‑1 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:1909701/140‑1 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:2698963/140‑1 (MQ=255)

                                                cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:4558561/140‑1 (MQ=255)

                                                 ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑TCCCCCCCGTACGGGAATTGGGCTGGTGACCGCTGATATCCCCCAGCGTcat                                                                                  >  1:992218/1‑138 (MQ=255)

                                                 ggTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCtt                                                                                  >  1:992144/1‑139 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGAGCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:1957190/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  1:99780/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:416029/1‑140 (MQ=255)

                                                    aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:1304769/1‑140 (MQ=255)

                                                     gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑TCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGGCAGCATCTGACGc                                                                              >  1:5092963/1‑140 (MQ=255)

                                                     gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCACCCTGTACGGTAATCGAGCAGCTGGCCACTGATTTCCGCCCGCATCTGACGc                                                                              >  1:5092962/1‑140 (MQ=255)

                                                      ttGCGGCCACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             >  2:3613662/1‑140 (MQ=255)

                                                      ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCGGATATCCGCCAGCATCTGACGcc                                                                             >  2:3038352/1‑140 (MQ=255)

                                                      ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTgccgcc                                                                             >  1:3701048/1‑140 (MQ=255)

                                                      ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTAACCGCTGATATCCGCCAGCATCTGACGcc                                                                             >  2:3751055/1‑140 (MQ=255)

                                                      ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGACCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCC‑‑TACGGTAATCGAGCCGCTGACCGCCGACATCCCCCACCCTCTGACGcc                                                                             >  1:4550801/1‑140 (MQ=255)

                                                       tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                            <  1:5338840/140‑1 (MQ=255)

                                                       tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                            <  2:1687296/140‑1 (MQ=255)

                                                       tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                            <  1:5338228/140‑1 (MQ=255)

                                                             aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                      <  2:2712459/140‑1 (MQ=255)

                                                                 aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                  <  2:959644/140‑1 (MQ=255)

                                                                  ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                 >  1:4028300/1‑140 (MQ=255)

                                                                   tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                                                                <  2:1974084/140‑1 (MQ=255)

                                                                        gAGCGATTTCTCTGGATTCGCGGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:3398088/140‑1 (MQ=255)

                                                                        gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  1:112813/140‑1 (MQ=255)

                                                                        gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:4007745/140‑1 (MQ=255)

                                                                        gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:3087478/140‑1 (MQ=255)

                                                                          gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                         >  1:1852743/1‑140 (MQ=255)

                                                                                tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  2:1985105/140‑1 (MQ=255)

                                                                                  tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGTTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:3657319/140‑1 (MQ=255)

                                                                                  tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:1217202/140‑1 (MQ=255)

                                                                                  tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:2818628/140‑1 (MQ=255)

                                                                                  tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:262009/140‑1 (MQ=255)

                                                                                  tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:2728562/140‑1 (MQ=255)

                                                                                         tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTa                                          <  1:66080/140‑1 (MQ=255)

                                                                                           gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  1:1102772/97‑1 (MQ=255)

                                                                                           gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   >  2:1102772/1‑97 (MQ=255)

                                                                                           gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                        >  1:1955302/1‑140 (MQ=255)

                                                                                                 ctCTGCGCCAGCCTTGCTGCGCCCAGTGCCGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:1165380/139‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGGCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:3539599/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:4796738/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:3361772/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:4797515/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:500374/140‑1 (MQ=255)

                                                                                                 aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:3305310/140‑1 (MQ=255)

                                                                                                      cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                             >  1:3910519/1‑140 (MQ=255)

                                                                                                            ccTTGCTGCGCCCAGTGCTGGCCCCGCATCCCCCCCCC‑‑TACGGGAAGCGAGCTGCTGACCGGTGATATCCGCCAGCCTCTGACGCCAGTACTCAGTACTGGCCCCGCCCCCAATCGACGTAACACTTTGCGGTTTAATag                       >  1:3023126/1‑139 (MQ=255)

                                                                                                            ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                       >  2:2379546/1‑140 (MQ=255)

                                                                                                                gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                   >  2:5337604/1‑140 (MQ=255)

                                                                                                                   gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                <  2:5018265/140‑1 (MQ=255)

                                                                                                                   gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                <  2:5018161/140‑1 (MQ=255)

                                                                                                                   gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAggg                >  1:79243/1‑139 (MQ=255)

                                                                                                                    cgcCCGGTGCTGTCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGACATCCGCCAGCATCTGACGCCAGTGCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa               <  2:1998657/140‑1 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGGGCTACTGACCGCTGCTACCCGCCAGCCTCTTACGCCAGCACTCACTGCGCGCCCCGCCCCCAATCAACTCAACACTTTGGGGTTTCATACCGGACGCATg             >  1:1172786/1‑140 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGAGTTAATACCGCACGCATg             >  1:2902104/1‑140 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg             >  2:2738214/1‑140 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGGCCCCCATCAACGTAACCCTGTGCGGGTTAGTACCGCAGGCATg             >  1:2902073/1‑140 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCCGCCCGCTGATATCCGCCAGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCACTCCACGTAAAACTTTTCGGTTTAATACCGCCGGccag             >  2:2738148/1‑137 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACAT‑‑CCCCCCACGTACGGTAATCGAGCTGCTGACCGCTCATATCGGTCAGCATTCGACGGCAGCACTCACTCCGCGCCCCGCCCCCAAGCAACGTCACACTTTGCGAGTGAATACCGAAGGCATg             >  2:4811821/1‑140 (MQ=255)

                                                                                                                      cccAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGGAATCGAGCTGGTGAACGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg             >  2:3244514/1‑140 (MQ=255)

                                                                                                                      cccACTGCTGGCCCCACAT‑‑CCCCCCCCGTACGCTAATCGAGCTGCCGACCGCTCACACCCGCCCGCCTCCGACCCCCCCACCCCCCCCCCCCCCCGCCCCCCCACAACCCGCCACCTCGCGGTCTAATACCGCactccac             >  2:5357242/1‑134 (MQ=255)

                                                                                                                           tGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATATGACGGCAGTACCCACTACGCGCCCCGCCCCCAACCAACGTAGCACTTTGCGGGTTAATACCGCAGGCATGCACgg        >  2:1933344/1‑139 (MQ=255)

                                                                                                                                gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCCCAGGCATGCACGACATcc   >  1:3173960/1‑140 (MQ=255)

                                                                                                                                gCCCCACAT‑‑CCCCCCCCGTACGGGAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  1:2717668/1‑140 (MQ=255)

                                                                                                                                 ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  2:1331617/140‑1 (MQ=255)

                                                                                                                                 ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  1:3972266/140‑1 (MQ=255)

||

CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728663‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  NZ_CP025268/3728669‑3728957

ccCGGCATAACGCTGCGCAGCTGGTAGATGCGACTGTGCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTCGCCCCACA‑‑GCCCCCCCCGTACGGTAATCG                                                                                                                                          <  SRR14920189.4126268/149‑1 (MQ=60)

AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCG                                                                                                                                          <  SRR14920189.4126702/151‑1 (MQ=60)

  CGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCTCCCCCGTACGGTAATCGAG                                                                                                                                        >  SRR14920189.2705012/1‑151 (MQ=60)

  CGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAG                                                                                                                                        >  SRR14920189.2705392/1‑151 (MQ=60)

  CGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAG                                                                                                                                        >  SRR14920189.2704995/1‑151 (MQ=60)

         ACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGA                                                                                                                                 >  SRR14920189.3598061/1‑151 (MQ=60)

         ACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGCACCGTAATCGAGCTGGTGA                                                                                                                                 >  SRR14920189.5126183/1‑151 (MQ=60)

                   TCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATAT                                                                                                                       >  SRR14920189.726592/1‑151 (MQ=60)

                              CGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGCTATTCGCCAGCATC                                                                                                            >  SRR14920189.5316585/1‑151 (MQ=60)

                              CGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCCAGCTGCTGACCGCTGATATCCGCCAGCATC                                                                                                            >  SRR14920189.3421966/1‑151 (MQ=60)

                                ACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑TCCCCCCCGTACGGGAATTGGGCTGGTGACCGCTGATATCCCCCAGCgtcat                                                                                                          >  SRR14920189.994533/1‑146 (MQ=60)

                                ACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTT                                                                                                          >  SRR14920189.994459/1‑151 (MQ=60)

                                   GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTAGGGTAATCGAGCTTCTGACCGCTGgtaaacgccagccgctgccg                                                                                                       >  SRR14920189.2715314/1‑131 (MQ=60)

                                   GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACG                                                                                                       <  SRR14920189.2051043/151‑1 (MQ=60)

                                   GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACG                                                                                                       <  SRR14920189.2051916/151‑1 (MQ=60)

                                   GTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACG                                                                                                       >  SRR14920189.100100/1‑151 (MQ=60)

                                    TTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑TCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGGCAGCATCTGACGC                                                                                                      >  SRR14920189.5105637/1‑151 (MQ=60)

                                    TTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCACCCTGTACGGTAATCGAGCAGCTGGCCACTGATTTCCGCCCGCATCTGACGC                                                                                                      >  SRR14920189.5105636/1‑151 (MQ=60)

                                     TCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGCCGCC                                                                                                     >  SRR14920189.3709699/1‑151 (MQ=60)

                                     TCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGACCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCC‑‑TACGGTAATCGAGCCGCTGACCGCCGACATCCCCCACCCTCTGACGCC                                                                                                     >  SRR14920189.4562095/1‑151 (MQ=60)

                                          ctcgtgggctcggagatgtgtataagagacagTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAC                                                                                                <  SRR14920189.1105295/119‑1 (MQ=60)

                                                 TGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAC                                                                                         <  SRR14920189.5351897/151‑1 (MQ=60)

                                                 TGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAC                                                                                         <  SRR14920189.5351283/151‑1 (MQ=60)

                                                 TGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAC                                                                                         >  SRR14920189.4038187/1‑151 (MQ=60)

                                                         CAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCG                                                                                 >  SRR14920189.1856985/1‑151 (MQ=60)

                                                                  GAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATC                                                                        <  SRR14920189.113157/151‑1 (MQ=60)

                                                                          TCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAAC                                                                >  SRR14920189.1959796/1‑151 (MQ=60)

                                                                            TCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACAC                                                              <  SRR14920189.1219967/151‑1 (MQ=60)

                                                                                   TCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGG                                                       <  SRR14920189.66298/151‑1 (MQ=60)

                                                                                     GCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGCAACACCTTGCGGTT                                                     >  SRR14920189.3919972/1‑151 (MQ=60)

                                                                                           ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCGCATCCCCCCCCC‑‑TACGGGAAGCGAGCTGCTGACCGGTGATATCCGCCAGCCTCTGACGCCAGTACTCAGTACTGGCCCCGCCCCCAATCGACGTAACACTTTGCGGTTTAATAG                                               >  SRR14920189.3030295/1‑151 (MQ=60)

                                                                                           ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                               <  SRR14920189.3313256/151‑1 (MQ=60)

                                                                                           ATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAC                                               <  SRR14920189.501655/151‑1 (MQ=60)

                                                                                                  CCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGG                                        >  SRR14920189.79501/1‑151 (MQ=60)

                                                                                                     GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGGGCTACTGACCGCTGCTACCCGCCAGCCTCTTACGCCAGCACTCACTGCGCGCCCCGCCCCCAATCAACTCAACACTTTGGGGTTTCATACCGGACGCATG                                     >  SRR14920189.1175447/1‑151 (MQ=60)

                                                                                                     GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGAGTTAATACCGCACGCATG                                     >  SRR14920189.2908590/1‑151 (MQ=60)

                                                                                                     GCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATAGCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGGCCCCCATCAACGTAACCCTGTGCGGGTTAGTACCGCAGGCATG                                     >  SRR14920189.2908557/1‑151 (MQ=60)

                                                                                                               GCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCCCAGGCATGCACGACATCC                           >  SRR14920189.3181491/1‑151 (MQ=60)

                                                                                                               GCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGCGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC                           >  SRR14920189.2723728/1‑151 (MQ=60)

                                                                                                                    GTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTAATCGAGCCGCTGACCGCTGATATTCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCTCCCCCAATCAACGTAACACTTTTCGGTTTAATAACGCAGGCATGCACGACATCCATGCC                      >  SRR14920189.2663494/1‑151 (MQ=60)

                                                                                                                    GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATCTCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCACCCCCAATCAACGTAACACTTTGCGGTTTAATACCGGAGGCAAGCACGACATCCATGCC                      >  SRR14920189.2964167/1‑151 (MQ=60)

                                                                                                                    GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATActgtctcttatacacatctccgagcccacgagactgcagctaatcccgtatgccgtcttctgcttgaaaaaacagcaattccggtgctgtcatcaaag                      >  SRR14920189.3582911/1‑53 (MQ=60)

                                                                                                                    GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGGCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAGGCACGACATCCATGCC                      >  SRR14920189.2964080/1‑151 (MQ=60)

                                                                                                                    GTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCCCCGGCATGCACGACATCCATGCC                      >  SRR14920189.5311721/1‑151 (MQ=60)

                                                                                                                    GTGCTGGCCCCACA‑‑GCCCCCCCCTTACGGGAATCGAGCTGCTGACCGCTGATATCCTCCAGCGTCTGACGCCAGTTCGCCCTGCGCGCCCCGCCCCCAGGCAACGGAACACTATGCGGGTTAATACCGGTGGCATTCACGACATACATGCC                      >  SRR14920189.2173214/1‑151 (MQ=42)

                                                                                                                           CCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCC               <  SRR14920189.3982079/151‑1 (MQ=60)

                                                                                                                                  catCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCCCCAGCATCTGACGCCCGTACCCCCCACGCGCCCCGCCCCCCATCAACGTAACCCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCACGCCATCTGCCcgccc          >  SRR14920189.4057278/4‑146 (MQ=60)

                                                                                                                                  catCCCCCCCCGTACGGTAATCGAGCGGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCCCTCTGCCAGCGC          >  SRR14920189.2215356/4‑151 (MQ=60)

                                                                                                                                   atCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCA         <  SRR14920189.3058401/149‑1 (MQ=60)

                                                                                                                                   atCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCA         >  SRR14920189.882304/3‑151 (MQ=60)

                                                                                                                                     CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATA       >  SRR14920189.2984284/1‑151 (MQ=60)

                                                                                                                                       CCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTAGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAC     >  SRR14920189.4793930/1‑151 (MQ=60)

                                                                                                                                        CCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACC    >  SRR14920189.198839/1‑151 (MQ=60)

                                                                                                                                          CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  SRR14920189.1592920/1‑151 (MQ=60)

                                                                                                                                          CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  SRR14920189.1592455/1‑151 (MQ=60)

                                                                                                                                          CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  SRR14920189.3093328/1‑151 (MQ=60)

                                                                                                                                          CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  SRR14920189.1195692/1‑151 (MQ=60)

                                                                                                                                          CCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  SRR14920189.3663096/1‑151 (MQ=60)

AGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCA  >  NZ_CP025268/3728669‑3728957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`