Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F15 I1 R4 781 701.7 22683696 98.1% 22252705 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,633,505 A→G *64* (TAA→TAG)  yfgG → YfgG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,633,5050AG100.0% 532.1 / NA 153*64* (TAA→TAG) yfgGYfgG family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/120);  total (33/120)

Only 100 of 153 total aligned reads displayed.

TATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC  >  NZ_CP025268/2633370‑2633637
                                                                                                                                       |                                                                                                                                    
tATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGt                                                                                                                                  >  1:10362606/1‑140 (MQ=255)
 aTCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGtt                                                                                                                                 <  1:8058283/140‑1 (MQ=255)
   cGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTAc                                                                                                                               <  2:2127208/140‑1 (MQ=255)
       cTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTtc                                                                                                                           <  2:8613195/140‑1 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTa                                                                                                                                >  2:2432313/1‑132 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTa                                                                                                                                >  2:2432134/1‑132 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTa                                                                                                                                <  1:2432313/132‑1 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTcc                                                                                                                        >  2:4966770/1‑140 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTcc                                                                                                                        >  1:1616798/1‑140 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTcc                                                                                                                        >  1:727433/1‑140 (MQ=255)
          cTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTcc                                                                                                                        >  1:2493030/1‑140 (MQ=255)
             atcaGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACt                                                                                                                     >  1:4131433/1‑140 (MQ=255)
             atcaGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCAAGATCAGTGCTAAAGAGACGCTCAGCTATCCACCCTCTCAGTCGAATCAGCGGTCGACAGTTAGCTGTTACCTtctcatct                                                                                                                     >  2:9994316/1‑136 (MQ=255)
              tcaGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACtt                                                                                                                    <  1:7757296/140‑1 (MQ=255)
                 gCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTca                                                                                                                 <  2:9451408/140‑1 (MQ=255)
                 gCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTca                                                                                                                 <  2:1898845/140‑1 (MQ=255)
                  cTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTcac                                                                                                                >  1:9350786/1‑140 (MQ=255)
                   tttATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTcaca                                                                                                               <  1:4828028/140‑1 (MQ=255)
                   tttATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTcaca                                                                                                               <  1:4075906/140‑1 (MQ=255)
                     tATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGa                                                                                                             >  2:5969750/1‑140 (MQ=255)
                      aTCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCNCACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCCCCACTNTCACAGaa                                                                                                            <  1:5999411/140‑1 (MQ=255)
                      aTCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGaa                                                                                                            <  1:8929618/140‑1 (MQ=255)
                      aTCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGaa                                                                                                            <  2:6743412/140‑1 (MQ=255)
                      aTCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGaa                                                                                                            <  2:8794339/140‑1 (MQ=255)
                            ttcttTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAAc                                                                                                      >  2:889356/1‑140 (MQ=255)
                                 tGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCAc                                                                                                 >  2:6874164/1‑140 (MQ=255)
                                     cGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCg                                                                                             <  2:7020753/140‑1 (MQ=255)
                                     cGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCg                                                                                             <  1:115753/140‑1 (MQ=255)
                                           aTCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCg                                                                                       <  2:790492/140‑1 (MQ=255)
                                           aTCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCg                                                                                       <  1:2316915/140‑1 (MQ=255)
                                             cTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                     <  1:116871/140‑1 (MQ=255)
                                             cTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                     <  1:10629417/140‑1 (MQ=255)
                                             cTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGa                                                                                     <  2:10876023/140‑1 (MQ=255)
                                              tACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGAt                                                                                    <  1:1441717/140‑1 (MQ=255)
                                                 tCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCt                                                                                 >  1:7257452/1‑140 (MQ=255)
                                                   gtccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCttt                                                                               <  2:6331123/140‑1 (MQ=255)
                                                     ccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                             <  2:3821453/140‑1 (MQ=255)
                                                     ccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                             <  2:5831080/140‑1 (MQ=255)
                                                     ccgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTg                                                                             <  2:1738146/140‑1 (MQ=255)
                                                     ccgtcGGTGCCTGGCAGCAACATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTTACTTCTCCACTTTCACATAACATATCGGCACTTCGCTTTCGGATGCTTTTg                                                                             <  2:11137825/140‑1 (MQ=255)
                                                      cgtcGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGAACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTAGCTGTCGGATGCTATTga                                                                            >  2:4587690/1‑139 (MQ=255)
                                                         cGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGt                                                                         <  2:9752672/140‑1 (MQ=255)
                                                                     gcaCCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCa                                                             >  1:5701374/1‑140 (MQ=255)
                                                                      caCCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCaa                                                            <  2:2336283/140‑1 (MQ=255)
                                                                         cATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGc                                                         <  2:727433/140‑1 (MQ=255)
                                                                          aTCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCg                                                        >  1:3732295/1‑140 (MQ=255)
                                                                          aTCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCg                                                        <  2:9264489/140‑1 (MQ=255)
                                                                          aTCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCg                                                        <  2:9264513/140‑1 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCa                                                                                                  <  1:1762053/97‑1 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCa                                                                                                  >  2:1766017/1‑97 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCa                                                                                                  <  1:1766017/97‑1 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCgg                                                       <  1:496153/140‑1 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCgg                                                       >  1:7096396/1‑140 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCgg                                                       >  1:7916705/1‑140 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCgg                                                       <  2:1678385/140‑1 (MQ=255)
                                                                           tCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCgg                                                       <  2:103135/140‑1 (MQ=255)
                                                                                gCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGat                                                  >  1:4165885/1‑140 (MQ=255)
                                                                                      aagaagCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCtt                                            <  1:2423835/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATTCTTTTGATGTTTGGGATTATCAAAGCGGTAGATATTCTTTCATCTTAAATTTTa                             <  1:4587690/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTTATCTTAAATTTTa                             <  1:9719460/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                             <  1:640424/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                             <  2:1397087/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                             <  2:9123189/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                             <  2:4911999/140‑1 (MQ=255)
                                                                                                     ccACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                             <  2:4656193/140‑1 (MQ=255)
                                                                                                       acacTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAACGCGGCAGATATTCTTTCATCTTAAATTTTACg                           <  1:5165474/140‑1 (MQ=255)
                                                                                                         acTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTc                         <  2:7257452/140‑1 (MQ=255)
                                                                                                         acTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTc                         <  2:3246793/140‑1 (MQ=255)
                                                                                                         acTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTc                         <  1:889356/140‑1 (MQ=255)
                                                                                                         acTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTc                         <  2:10362606/140‑1 (MQ=255)
                                                                                                          ctctCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCt                        <  2:363263/140‑1 (MQ=255)
                                                                                                          ctctCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCt                        <  2:3183928/140‑1 (MQ=255)
                                                                                                              ccGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTAt                    <  1:4691805/140‑1 (MQ=255)
                                                                                                               cGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTa                             >  2:5512492/1‑130 (MQ=255)
                                                                                                               cGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATc                   >  1:839561/1‑140 (MQ=255)
                                                                                                                 tCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCct                 <  2:6867429/140‑1 (MQ=255)
                                                                                                                   gAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATACTTTCATCTTAAATTTTACGTCTTTATCctga               <  2:9597070/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATACCGGCACTTCGCGGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  2:8374151/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  1:10322763/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  1:34922/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  2:9350786/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  2:955331/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  1:4224129/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCctgact             <  1:6939686/140‑1 (MQ=255)
                                                                                                                     aTCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTGATCctgact             <  2:10614605/140‑1 (MQ=255)
                                                                                                                     aTCAACGTTACAACGTTAGCGGTGAGGTTCTCCTCATTCACAGACCTTTACGGCTCTTCGCTGTCGGTTGGTTTTGCTTTTTGGGATTATATATGGGGCTGATTTTCTTTCTTCATAAATTTTACGTCTTTATCctgact             <  2:3567545/140‑1 (MQ=255)
                                                                                                                         ccGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATg         >  1:5469094/1‑140 (MQ=255)
                                                                                                                            gTACAACGTTCGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTT‑GGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  1:5684658/140‑1 (MQ=255)
                                                                                                                             tACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  1:10959842/140‑1 (MQ=255)
                                                                                                                             tACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  1:2260173/140‑1 (MQ=255)
                                                                                                                             tACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  2:1181761/140‑1 (MQ=255)
                                                                                                                             tACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  1:5858568/140‑1 (MQ=255)
                                                                                                                             tACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  1:3846905/140‑1 (MQ=255)
                                                                                                                               cAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATc   <  2:9225627/140‑1 (MQ=255)
                                                                                                                               cAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATc   <  2:2595227/140‑1 (MQ=255)
                                                                                                                               cAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATc   <  1:2119539/140‑1 (MQ=255)
                                                                                                                               cAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTCATCCTGACTGATGTTTATc   <  2:2970057/140‑1 (MQ=255)
                                                                                                                                aaCGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATcc  <  2:1013684/140‑1 (MQ=255)
                                                                                                                                aaCGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATcc  <  2:1013683/140‑1 (MQ=255)
                                                                                                                                    ttAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTa     <  2:9777586/133‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
TATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC  >  NZ_CP025268/2633370‑2633637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAA  >  NZ_CP025268/2633358‑2633655
                                                                                                                                                   |                                                                                                                                                      
TCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGG                                                                                                                                                     <  SRR14920199.6521541/151‑1 (MQ=60)
TCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGG                                                                                                                                                     <  SRR14920199.8257334/151‑1 (MQ=60)
TCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGG                                                                                                                                                     <  SRR14920199.3241350/151‑1 (MQ=60)
 CGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGT                                                                                                                                                    >  SRR14920199.10409743/1‑151 (MQ=60)
     TGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACC                                                                                                                                                <  SRR14920199.6495021/151‑1 (MQ=60)
      GACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCT                                                                                                                                               <  SRR14920199.7794526/151‑1 (MQ=60)
           GTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCC                                                                                                                                          >  SRR14920199.1623804/1‑151 (MQ=60)
           GTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCC                                                                                                                                          >  SRR14920199.5730048/1‑151 (MQ=60)
           GTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCC                                                                                                                                          >  SRR14920199.730669/1‑151 (MQ=60)
           GTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCC                                                                                                                                          >  SRR14920199.2503869/1‑151 (MQ=60)
             ATCGTACTGCTCATCAGCTTTATCTTCTTCTCTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCAC                                                                                                                                        <  SRR14920199.8094653/151‑1 (MQ=60)
              TCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACT                                                                                                                                       >  SRR14920199.4149267/1‑151 (MQ=60)
              TCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACT                                                                                                                                       <  SRR14920199.2442929/151‑1 (MQ=60)
              TCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACT                                                                                                                                       <  SRR14920199.2442749/151‑1 (MQ=60)
                   CTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCAC                                                                                                                                  >  SRR14920199.9393207/1‑151 (MQ=60)
                          TCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACAT                                                                                                                           <  SRR14920199.7792140/151‑1 (MQ=60)
                               TTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGG                                                                                                                      <  SRR14920199.4848818/151‑1 (MQ=60)
                               TTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGG                                                                                                                      <  SRR14920199.4093475/151‑1 (MQ=60)
                                  ATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCNCACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCCCCACTNTCACAGAACATAACGGCAC                                                                                                                   <  SRR14920199.6025635/151‑1 (MQ=60)
                                  ATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCAC                                                                                                                   <  SRR14920199.8970043/151‑1 (MQ=60)
                                        TTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTGACCTGCTCCACTTTCACAGgccataccggcactgcgcg                                                                                                             >  SRR14920199.7245384/1‑132 (MQ=60)
                                            ctcgtgggctcggagatgtgtataagagacagTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCG                                                                                                         <  SRR14920199.1769601/119‑1 (MQ=60)
                                            ctcgtgggctcggagatgtgtataagagacagTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCG                                                                                                         <  SRR14920199.1773582/119‑1 (MQ=60)
                                                 CGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGC                                                                                                    <  SRR14920199.116368/151‑1 (MQ=60)
                                                  GTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCT                                                                                                   >  SRR14920199.7289896/1‑151 (MQ=60)
                                                       ATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGC                                                                                              <  SRR14920199.2327014/151‑1 (MQ=60)
                                                       ATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGC                                                                                              <  SRR14920199.2326986/151‑1 (MQ=60)
                                                         CTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTG                                                                                            <  SRR14920199.117491/151‑1 (MQ=60)
                                                         CTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTG                                                                                            <  SRR14920199.9250764/151‑1 (MQ=60)
                                                         CTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTG                                                                                            <  SRR14920199.10677805/151‑1 (MQ=60)
                                                          TACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGT                                                                                           <  SRR14920199.1447980/151‑1 (MQ=60)
                                                                 CCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGAT                                                                                    <  SRR14920199.10561536/151‑1 (MQ=60)
                                                                 CCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGAT                                                                                    <  SRR14920199.1667189/151‑1 (MQ=60)
                                                                 CCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGAT                                                                                    <  SRR14920199.11341133/151‑1 (MQ=60)
                                                                      GGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCA                                                                               >  SRR14920199.5726126/1‑151 (MQ=60)
                                                                           CTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCG                                                                          >  SRR14920199.3748390/1‑151 (MQ=60)
                                                                            TGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGG                                                                         >  SRR14920199.7952377/1‑151 (MQ=60)
                                                                            TGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGG                                                                         >  SRR14920199.7127993/1‑151 (MQ=60)
                                                                                 GCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGAT                                                                    >  SRR14920199.4183850/1‑151 (MQ=60)
                                                                                 GCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGAT                                                                    >  SRR14920199.4183843/1‑151 (MQ=60)
                                                                                       TCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTT                                                              <  SRR14920199.498428/151‑1 (MQ=60)
                                                                                                  AAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAAT                                                   <  SRR14920199.2434417/151‑1 (MQ=60)
                                                                                                                TCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATC                                     >  SRR14920199.1239902/1‑151 (MQ=60)
                                                                                                                TCCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATC                                     >  SRR14920199.843270/1‑151 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATTCTTTTGATGTTTGGGATTATCAAAGCGGTAGATATTCTTTCATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.4607471/151‑1 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTTATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.9763531/151‑1 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.6904691/151‑1 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.5536420/151‑1 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.3898514/151‑1 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.6297355/151‑1 (MQ=60)
                                                                                                                 CCACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCC                                    <  SRR14920199.643299/151‑1 (MQ=60)
                                                                                                                   ACACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAACGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTG                                  <  SRR14920199.5187828/151‑1 (MQ=60)
                                                                                                                     ACTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGAC                                <  SRR14920199.893284/151‑1 (MQ=60)
                                                                                                                      CTCTCCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACT                               >  SRR14920199.502108/1‑151 (MQ=60)
                                                                                                                          CCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATG                           <  SRR14920199.4712038/151‑1 (MQ=60)
                                                                                                                          CCGTCGAATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATG                           >  SRR14920199.5492836/1‑151 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.7369240/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.190739/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.10369730/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.3039113/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.35104/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.6970510/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.35131/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.4242326/151‑1 (MQ=60)
                                                                                                                                 ATCACCGGTACAACGTTAGCGGTTACCTTCTCCACTATCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCC                    <  SRR14920199.4816875/151‑1 (MQ=60)
                                                                                                                                     CCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTT                <  SRR14920199.887308/151‑1 (MQ=60)
                                                                                                                                     CCGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTT                >  SRR14920199.9821948/1‑151 (MQ=60)
                                                                                                                                      CGGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTT               <  SRR14920199.8065728/151‑1 (MQ=60)
                                                                                                                                       GGTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTG              >  SRR14920199.9427923/1‑151 (MQ=60)
                                                                                                                                        GTACAACGTTCGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTT‑GGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGC            <  SRR14920199.5709334/151‑1 (MQ=60)
                                                                                                                                        GTACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGG             >  SRR14920199.9322945/1‑151 (MQ=60)
                                                                                                                                         TACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGC            <  SRR14920199.5884024/151‑1 (MQ=60)
                                                                                                                                         TACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGC            <  SRR14920199.3863465/151‑1 (MQ=60)
                                                                                                                                         TACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGC            <  SRR14920199.2270043/151‑1 (MQ=60)
                                                                                                                                         TACAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGC            <  SRR14920199.11009986/151‑1 (MQ=60)
                                                                                                                                           CAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTG          <  SRR14920199.4550889/151‑1 (MQ=60)
                                                                                                                                           CAACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTG          <  SRR14920199.2128762/151‑1 (MQ=60)
                                                                                                                                             ACGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCctgtctcttatacacatctccgagccca        >  SRR14920199.2456878/1‑123 (MQ=60)
                                                                                                                                              CGTTAGCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCctgtctcttatac       >  SRR14920199.9491765/1‑138 (MQ=60)
                                                                                                                                                   GCGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAA  <  SRR14920199.4051926/151‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                      
TCGCATGACCCGTATCGTACTGCTCATCAGCTTTATCTTCTTCTTTGGCCGTTTTATCTACTCGTCCGTCGGTGCCTGGCAGCACCATCAGAGCAAAAAAGAAGCTCAGCAATCCACACTCTCCGTCGAATCACCGGTACAACGTTAACGGTTACCTTCTCCACTTTCACAGAACATAACGGCACTTCGCTGTCGGATGCTTTTGCTGTTTGGGATTATCAAAGCGGCAGATATTCTTTCATCTTAAATTTTACGTCTTTATCCTGACTGATGTTTATCCTGTTTGGCTGCGAAATAA  >  NZ_CP025268/2633358‑2633655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑