Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R1	782	316.7	10427156	97.0%	10114341	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	820,054	A→G	226 (TAA→TAG)	bioD →	dethiobiotin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	820,054	0	A	G	100.0%	306.6 / NA	93	226 (TAA→TAG)	bioD	dethiobiotin synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (39/54); total (39/54)

CCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGC  >  NZ_CP025268/819917‑820188

ccGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGcc                                                                                                                                      >  1:1808732/1‑140 (MQ=255)

ccGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGcc                                                                                                                                      >  2:1980151/1‑140 (MQ=255)

ccGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGcc                                                                                                                                      >  2:1352683/1‑140 (MQ=255)

ccGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGGTGTAGcc                                                                                                                                      >  2:17221/1‑140 (MQ=255)

   ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                   <  2:3300198/140‑1 (MQ=255)

   ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                   <  2:3300176/140‑1 (MQ=255)

   ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                   <  2:1996280/140‑1 (MQ=255)

   ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                   <  1:2526347/140‑1 (MQ=255)

   ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                   <  1:2743955/140‑1 (MQ=255)

   ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                   <  1:4573724/140‑1 (MQ=255)

        aCGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTa                                                                                                                              <  2:4151699/140‑1 (MQ=255)

        aCGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTa                                                                                                                              <  1:55275/140‑1 (MQ=255)

          gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                            <  1:613281/140‑1 (MQ=255)

          gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                            <  1:3242483/140‑1 (MQ=255)

          gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                            <  1:2762675/140‑1 (MQ=255)

          gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                            <  1:92419/140‑1 (MQ=255)

          gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                            <  1:1375732/140‑1 (MQ=255)

                cTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTa                                                                                                                      <  1:1045153/140‑1 (MQ=255)

                        atGACCACGCTCCCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCGCCTGGCTTGCAGAAAATCCAGAAAATGCGTCAACCGGAAAGTACATAAACCTGGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                              <  2:1761252/140‑1 (MQ=255)

                        atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                              >  2:2882060/1‑140 (MQ=255)

                        atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                              <  1:242715/140‑1 (MQ=255)

                        atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                              <  2:3619933/140‑1 (MQ=255)

                        atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGACTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                              >  2:2882448/1‑140 (MQ=255)

                         tGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcgag                                                                                                             <  1:1129131/140‑1 (MQ=255)

                           aCCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATACACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcgagcg                                                                                                           >  2:330517/1‑140 (MQ=255)

                             cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGcgagcgag                                                                                                         >  1:4382045/1‑140 (MQ=255)

                             cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGcgagcgag                                                                                                         >  1:3927635/1‑140 (MQ=255)

                             cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGcgagcgag                                                                                                         >  2:3371508/1‑140 (MQ=255)

                             cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGcgagcgag                                                                                                         >  1:1202480/1‑140 (MQ=255)

                               cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTGCATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                       >  2:2790691/1‑140 (MQ=255)

                               cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                       >  2:2527294/1‑140 (MQ=255)

                               cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                       >  1:374106/1‑140 (MQ=255)

                               cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                       >  1:1114479/1‑140 (MQ=255)

                               cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                       >  1:2935045/1‑140 (MQ=255)

                               cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                       >  1:1187756/1‑140 (MQ=255)

                                gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                      <  1:1477866/140‑1 (MQ=255)

                                gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                      <  1:1477667/140‑1 (MQ=255)

                                    aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                  <  1:2188034/140‑1 (MQ=255)

                                    aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                  <  1:2127510/140‑1 (MQ=255)

                                    aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                  <  1:2127453/140‑1 (MQ=255)

                                     cccGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTc                                                                                                 >  1:1278903/1‑140 (MQ=255)

                                     cccGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTc                                                                                                 >  1:863357/1‑140 (MQ=255)

                                          aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGa                                                                                            >  1:1692757/1‑140 (MQ=255)

                                          aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGa                                                                                            >  2:4415919/1‑140 (MQ=255)

                                          aTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGa                                                                                            >  2:2660940/1‑140 (MQ=255)

                                            gATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                          <  1:1683185/140‑1 (MQ=255)

                                            gATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                          <  2:5064649/140‑1 (MQ=255)

                                            gATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGGTGTAGCCATTCTGGATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                          <  1:330517/140‑1 (MQ=255)

                                              ttCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGa                                                                                        >  1:5148309/1‑140 (MQ=255)

                                               tCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                       <  1:166495/140‑1 (MQ=255)

                                               tCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                       <  2:4686329/140‑1 (MQ=255)

                                                cccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                                      <  1:3741516/140‑1 (MQ=255)

                                                               ggAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAAtt                                                                       <  2:456548/140‑1 (MQ=255)

                                                                gagagcTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                                      <  2:4568028/140‑1 (MQ=255)

                                                                gagagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTTACCAATTa                                                                      <  2:3678040/140‑1 (MQ=255)

                                                                 agagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGGAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCTAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaa                                                                     >  1:53431/1‑140 (MQ=255)

                                                                 agagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTACCCAATTaa                                                                     >  1:53565/1‑140 (MQ=255)

                                                                 agagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGATTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaa                                                                     >  1:3710811/1‑140 (MQ=255)

                                                                  gagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTCGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTaaa                                                                    <  1:2847765/140‑1 (MQ=255)

                                                                   agaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAt                                                                   >  1:3181209/1‑140 (MQ=255)

                                                                    gaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCGTCGATTGACTGCAATTTAACCAATTAAAtt                                                                  >  1:2606031/1‑140 (MQ=255)

                                                                    gaTCCCCTGGCTAGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAtt                                                                  >  2:5135529/1‑140 (MQ=255)

                                                                         ccTGGCTTGCAGAAACTCCAGCAAATGCGGCAACCGGAAAGTACATAAACCTCGCCTTGGTGCAGCCATTCTGTATTTGGATAAATTGCGAGCGAGCTCGCGTGTTCGATTAACTTCAATTTAACCCATTAAATTCTaaa                                                             >  2:3865902/1‑140 (MQ=255)

                                                                                  cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACg                                                    >  1:1767866/1‑140 (MQ=255)

                                                                                   aGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGGTGTAGCCATTCTGTATTTGGGTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGa                                                   >  2:798186/1‑140 (MQ=255)

                                                                                       aaTCCATAATATGCGGCAACCGCAAAGTACCTAAACCTTGACTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGTTTCTTGGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGaaaaa                                               <  2:4076648/140‑1 (MQ=255)

                                                                                       aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGaaaaa                                               <  1:1568678/140‑1 (MQ=255)

                                                                                       aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGaaaaa                                               <  1:1569058/140‑1 (MQ=255)

                                                                                                aaTGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACt                                      <  1:251429/140‑1 (MQ=255)

                                                                                                aaTGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACt                                      <  1:1039962/140‑1 (MQ=255)

                                                                                                   gCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTcc                                   <  2:4857404/140‑1 (MQ=255)

                                                                                                   gCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATCTTACTTcc                                   <  1:1061268/140‑1 (MQ=255)

                                                                                                      gCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGcc                                <  2:2273840/140‑1 (MQ=255)

                                                                                                       cAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCt                               <  1:2011944/140‑1 (MQ=255)

                                                                                                        aaCCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTc                              >  2:4425236/1‑140 (MQ=255)

                                                                                                        aaCCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTACGCCTc                              >  2:3360956/1‑140 (MQ=255)

                                                                                                         aCCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCa                             <  1:4268983/140‑1 (MQ=255)

                                                                                                         aCCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCa                             <  1:345102/140‑1 (MQ=255)

                                                                                                             gAAACTAAAGAAACCGTGTCTTGGTGTAGCCATTCTGTATTTGTCTAAACTGCGATCTAGATCGCGTCTTCGATTGACTGCTATTTAACCCACTAAATTCTAGAATAATCTCTAAAAAAATTTTACTTCCGCCTCCTgcg                         <  2:3992144/140‑1 (MQ=255)

                                                                                                               aaGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                       <  2:4372550/140‑1 (MQ=255)

                                                                                                               aaGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                       <  1:988715/140‑1 (MQ=255)

                                                                                                               aaGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                       <  2:2994617/140‑1 (MQ=255)

                                                                                                               aaGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggc                       <  2:1187756/140‑1 (MQ=255)

                                                                                                                aGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggcg                      <  1:3871882/140‑1 (MQ=255)

                                                                                                                    cATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAAtg                  >  1:1501298/1‑140 (MQ=255)

                                                                                                                          ccttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGaa            <  1:4994922/140‑1 (MQ=255)

                                                                                                                          ccttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGaa            <  2:757004/140‑1 (MQ=255)

                                                                                                                            ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAtt          >  2:1116228/1‑140 (MQ=255)

                                                                                                                            ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAtt          >  1:3129642/1‑140 (MQ=255)

                                                                                                                            ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAAGCACGAAAAAAATTTTACTTCCGCCTCATGCTGCGAATGTGGGAAtt          >  1:1893533/1‑140 (MQ=255)

                                                                                                                               ccttgTTGTAGCCCTTCTGTATTTGGTTAAATTGCCAGCGAGATCGCGTCTTCGATTGACTGCCATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGcc       <  1:2850654/140‑1 (MQ=255)

                                                                                                                               ccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGcc       <  2:4869793/140‑1 (MQ=255)

                                                                                                                                    ttgtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAggc  >  1:3336441/1‑140 (MQ=255)

CCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGC  >  NZ_CP025268/819917‑820188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAT  >  NZ_CP025268/819905‑820198

GATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGC                                                                                                                                                 <  SRR14920191.3810569/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.3311259/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.2148107/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.2148534/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.2446276/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.2631158/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                >  SRR14920191.1813640/1‑151 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                >  SRR14920191.1813622/1‑151 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.3027388/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.3390974/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.4388029/151‑1 (MQ=60)

 ATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAAGCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCC                                                                                                                                                <  SRR14920191.4489116/151‑1 (MQ=60)

     TACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTC                                                                                                                                            <  SRR14920191.2050833/151‑1 (MQ=60)

          CTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAT                                                                                                                                       <  SRR14920191.338644/151‑1 (MQ=60)

          CTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAT                                                                                                                                       <  SRR14920191.4895020/151‑1 (MQ=60)

          CTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAT                                                                                                                                       <  SRR14920191.4933474/151‑1 (MQ=60)

          CTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAT                                                                                                                                       <  SRR14920191.1974733/151‑1 (MQ=60)

               GGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGT                                                                                                                                  <  SRR14920191.2751278/151‑1 (MQ=60)

               GGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGT                                                                                                                                  <  SRR14920191.4587156/151‑1 (MQ=60)

               GGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGT                                                                                                                                  <  SRR14920191.2533095/151‑1 (MQ=60)

                    ACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAAT                                                                                                                             <  SRR14920191.55474/151‑1 (MQ=60)

                      GTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTG                                                                                                                           <  SRR14920191.92720/151‑1 (MQ=60)

                      GTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTG                                                                                                                           <  SRR14920191.615037/151‑1 (MQ=60)

                      GTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTG                                                                                                                           <  SRR14920191.2770053/151‑1 (MQ=60)

                      GTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTG                                                                                                                           <  SRR14920191.3251736/151‑1 (MQ=60)

                      GTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTG                                                                                                                           <  SRR14920191.1379433/151‑1 (MQ=60)

                            CTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCG                                                                                                                     <  SRR14920191.1047953/151‑1 (MQ=60)

                              GAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAG                                                                                                                   >  SRR14920191.1205690/1‑151 (MQ=60)

                              GAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAG                                                                                                                   >  SRR14920191.4394819/1‑151 (MQ=60)

                              GAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAG                                                                                                                   >  SRR14920191.3938931/1‑151 (MQ=60)

                                ATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAT                                                                                                                 >  SRR14920191.375185/1‑151 (MQ=60)

                                ATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAT                                                                                                                 >  SRR14920191.2943363/1‑151 (MQ=60)

                                ATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAT                                                                                                                 >  SRR14920191.1190931/1‑151 (MQ=60)

                                ATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAT                                                                                                                 >  SRR14920191.1117469/1‑151 (MQ=60)

                                    ATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCG                                                                                                             <  SRR14920191.243442/151‑1 (MQ=60)

                                     TGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGT                                                                                                            <  SRR14920191.1132159/151‑1 (MQ=60)

                                      GACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTC                                                                                                           >  SRR14920191.1282279/1‑151 (MQ=60)

                                      GACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTC                                                                                                           >  SRR14920191.865760/1‑151 (MQ=60)

                                           CGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGA                                                                                                      >  SRR14920191.1697355/1‑151 (MQ=60)

                                            GCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAT                                                                                                     <  SRR14920191.1481647/151‑1 (MQ=60)

                                            GCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAT                                                                                                     <  SRR14920191.1481846/151‑1 (MQ=60)

                                               CACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGA                                                                                                  >  SRR14920191.5163089/1‑151 (MQ=60)

                                                ACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAC                                                                                                 <  SRR14920191.2133290/151‑1 (MQ=60)

                                                ACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAC                                                                                                 <  SRR14920191.2133231/151‑1 (MQ=60)

                                                ACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAC                                                                                                 <  SRR14920191.2193966/151‑1 (MQ=60)

                                                        GATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTT                                                                                         <  SRR14920191.1687741/151‑1 (MQ=60)

                                                        GATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGGTGTAGCCATTCTGGATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTT                                                                                         <  SRR14920191.331491/151‑1 (MQ=60)

                                                           TCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAAC                                                                                      <  SRR14920191.166999/151‑1 (MQ=60)

                                                            CCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACC                                                                                     <  SRR14920191.3752029/151‑1 (MQ=60)

                                                                  CCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGGAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCTAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAA                                                                               >  SRR14920191.53623/1‑151 (MQ=60)

                                                                  CCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTACCCAATTAA                                                                               >  SRR14920191.53759/1‑151 (MQ=60)

                                                                  CCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGATTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAA                                                                               >  SRR14920191.3721214/1‑151 (MQ=60)

                                                                    GCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAT                                                                             >  SRR14920191.3190328/1‑151 (MQ=60)

                                                                     CTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCGTCGATTGACTGCAATTTAACCAATTAAATT                                                                            >  SRR14920191.2612970/1‑151 (MQ=60)

                                                                              GAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTCGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAA                                                                   <  SRR14920191.2855397/151‑1 (MQ=60)

                                                                                   CCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACG                                                              >  SRR14920191.1772666/1‑151 (MQ=60)

                                                                                                   AATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTC                                              <  SRR14920191.1572908/151‑1 (MQ=60)

                                                                                                   AATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTC                                              <  SRR14920191.1573288/151‑1 (MQ=60)

                                                                                                            AATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATG                                     <  SRR14920191.1042753/151‑1 (MQ=60)

                                                                                                            AATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATG                                     <  SRR14920191.252175/151‑1 (MQ=60)

                                                                                                               GCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATCTTACTTCCGCCTCATGCGG                                  <  SRR14920191.1064107/151‑1 (MQ=60)

                                                                                                                   CAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAA                              <  SRR14920191.2017430/151‑1 (MQ=60)

                                                                                                                     ACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATG                            <  SRR14920191.346110/151‑1 (MQ=60)

                                                                                                                     ACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATG                            >  SRR14920191.1505354/1‑151 (MQ=60)

                                                                                                                     ACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATG                            <  SRR14920191.4281348/151‑1 (MQ=60)

                                                                                                                           AAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAA                      <  SRR14920191.991389/151‑1 (MQ=60)

                                                                                                                            AGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAT                     <  SRR14920191.3883066/151‑1 (MQ=60)

                                                                                                                             GTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATT                    >  SRR14920191.3138676/1‑151 (MQ=60)

                                                                                                                             GTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAAGCACGAAAAAAATTTTACTTCCGCCTCATGCTGCGAATGTGGGAATT                    >  SRR14920191.1898652/1‑151 (MQ=60)

                                                                                                                                     ACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGC            >  SRR14920191.3345884/1‑151 (MQ=60)

                                                                                                                                      CCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCG           <  SRR14920191.5009390/151‑1 (MQ=60)

                                                                                                                                           CCTTGTTGTAGCCCTTCTGTATTTGGTTAAATTGCCAGCGAGATCGCGTCTTCGATTGACTGCCATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGG      <  SRR14920191.2858293/151‑1 (MQ=60)

                                                                                                                                               GTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCtgtctcttatacacatctccgagcccacgagaccgatcagtatctcgtatgccgtcttctgcttgaaaaaatgtgtcgtaaattgtggtttt  >  SRR14920191.2894003/1‑59 (MQ=60)

                                                                                                                                               GTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCtgtctcttatacacatctccgagcccacgagaccgatcagtatctcgtatgccgtcttctgcttgaaaaaagtgatgggacgcgggaggttg  >  SRR14920191.2894184/1‑59 (MQ=60)

                                                                                                                                               GTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAT  >  SRR14920191.1883753/1‑151 (MQ=60)

                                                                                                                                               GTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAT  >  SRR14920191.2011825/1‑151 (MQ=60)

GATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGGCGGGGGAT  >  NZ_CP025268/819905‑820198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`