Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R2	870	341.9	11209456	97.2%	10895591	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	400,598	+G	pseudogene (162/260 nt)	iraP →	anti‑adapter protein IraP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	400,594	1	.	G	97.6%	413.3 / 2.1	126	pseudogene (158/260 nt)	iraP	anti‑adapter protein IraP
Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (54/69); total (56/70)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400457‑400728

gttgttCTTTAAGCTTGCCCAAAAAGGAGAAGAGTCCAAAGAACTTTGTGCGCAGGTAGACGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg                                                                                                                                       <  2:553824/140‑1 (MQ=255)

gttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gg                                                                                                                                       <  1:2428298/140‑1 (MQ=255)

  tgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTagaatgg                                                                                                                                      >  1:466324/1‑136 (MQ=255)

    ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gggggc                                                                                                                                   <  1:542771/140‑3 (MQ=255)

     tATTTAAGCTTGCCCAAAACGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gggggcg                                                                                                                                  <  2:3734354/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gggggcg                                                                                                                                  <  1:3851482/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gggggcg                                                                                                                                  <  1:1811911/140‑4 (MQ=255)

     tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gggggcg                                                                                                                                  <  1:867149/140‑4 (MQ=255)

      aTTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑gggggcgc                                                                                                                                 >  1:4498027/1‑136 (MQ=255)

         tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGt                                                                                                                              >  1:1333644/1‑140 (MQ=255)

           aGCTTGCCCAAAAAGAAGACGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTATAGGGGG‑CGCTGTAc                                                                                                                            >  1:666742/1‑140 (MQ=255)

           aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTAc                                                                                                                            <  2:4910892/140‑1 (MQ=255)

            gCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACg                                                                                                                           <  1:2679542/140‑1 (MQ=255)

            gCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACg                                                                                                                           <  2:2883900/140‑1 (MQ=255)

            gCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACg                                                                                                                           <  2:2883979/140‑1 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTa                                                                                                                      >  1:893774/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTa                                                                                                                      >  2:1619884/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTa                                                                                                                      >  1:1408117/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTa                                                                                                                      >  1:3951694/1‑140 (MQ=255)

                 cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTAATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTa                                                                                                                      >  2:5079445/1‑140 (MQ=255)

                 cccAAAAAGAAAAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAACAGGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTa                                                                                                                      >  1:4639291/1‑140 (MQ=255)

                     aaaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCGTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGc                                                                                                                  <  2:2294304/140‑1 (MQ=255)

                      aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGcc                                                                                                                 <  2:3055127/140‑1 (MQ=255)

                       aagaagaagAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGccc                                                                                                                >  2:191661/1‑140 (MQ=255)

                          aagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCCATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGAt                                                                                                             <  1:4659324/140‑1 (MQ=255)

                                 gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  1:1185709/140‑1 (MQ=255)

                                 gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  1:4333603/140‑1 (MQ=255)

                                 gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  1:4333564/140‑1 (MQ=255)

                                 gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  1:4333497/140‑1 (MQ=255)

                                  tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCAt                                                                                                     >  1:3204202/1‑140 (MQ=255)

                                    gAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                   >  1:2538536/1‑140 (MQ=255)

                                          aCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg                                                                                             >  1:3644031/1‑140 (MQ=255)

                                           cTGTGTGCGCAGGTAGAAGGTTTGGAGATTATCGTCACTGCCATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  1:535348/140‑1 (MQ=255)

                                           cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:3141868/140‑1 (MQ=255)

                                           cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:1704864/140‑1 (MQ=255)

                                           cTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                            <  2:1704635/140‑1 (MQ=255)

                                              tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                         >  2:2117088/1‑140 (MQ=255)

                                                tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       <  2:1727485/140‑1 (MQ=255)

                                                tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  1:5328612/1‑140 (MQ=255)

                                                   gcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATGAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACgg                                                                                    >  2:5328218/1‑140 (MQ=255)

                                                   gcAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACgg                                                                                    >  2:3250471/1‑140 (MQ=255)

                                                     aGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAg                                                                                  <  2:3306897/140‑1 (MQ=255)

                                                        tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  1:982623/1‑140 (MQ=255)

                                                          gAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctgct                                                                             >  1:199758/1‑140 (MQ=255)

                                                          gAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctgct                                                                             >  2:1089390/1‑140 (MQ=255)

                                                             gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGTTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          <  2:2892244/140‑1 (MQ=255)

                                                             gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          <  1:3078537/140‑1 (MQ=255)

                                                             gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          <  2:3251645/140‑1 (MQ=255)

                                                             gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                          <  2:2890770/140‑1 (MQ=255)

                                                               tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        <  2:2012985/140‑1 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  1:2009009/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:5395101/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:1274412/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:1181891/1‑140 (MQ=255)

                                                                 tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGAGTGATCAGGTAGA‑GGGGTCGCTGGACGAGTTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:2662082/1‑140 (MQ=255)

                                                                 tGGAGAATATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATGAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCGGCGCGAt                                                                      >  1:4428325/1‑140 (MQ=255)

                                                                  ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  2:2953648/140‑1 (MQ=255)

                                                                  ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  1:2730041/140‑1 (MQ=255)

                                                                  ggAGAATATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  2:1764958/140‑1 (MQ=255)

                                                                       ttATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa                                                                <  2:3782693/140‑1 (MQ=255)

                                                                       ttATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa                                                                <  2:3782728/140‑1 (MQ=255)

                                                                           cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                            <  2:4755354/140‑1 (MQ=255)

                                                                           cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                            <  1:2146174/140‑1 (MQ=255)

                                                                           cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                            <  1:5353205/140‑1 (MQ=255)

                                                                             tCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag                                                          <  2:4327877/140‑1 (MQ=255)

                                                                               aCTGCAATGCTTCGCAATATGGCGCAAGATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACCTCAAGAAGtt                                                        >  2:4108585/1‑140 (MQ=255)

                                                                                   cAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                    <  2:2876916/140‑1 (MQ=255)

                                                                                   cAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTg                                                    <  2:5033490/140‑1 (MQ=255)

                                                                                      tgctgcGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAg                                                 >  2:5138925/1‑140 (MQ=255)

                                                                                      tGCTCCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAg                                                 >  2:5138699/1‑140 (MQ=255)

                                                                                      tGCTACGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAg                                                 >  2:5139048/1‑140 (MQ=255)

                                                                                        cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               >  1:1621341/1‑140 (MQ=255)

                                                                                        cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               >  2:1132322/1‑140 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  2:5535186/1‑140 (MQ=255)

                                                                                           cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  1:2707238/1‑140 (MQ=255)

                                                                                                    gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTa                                   <  1:4177475/140‑1 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  2:676015/1‑140 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:37205/1‑140 (MQ=255)

                                                                                                     cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:666698/1‑140 (MQ=255)

                                                                                                            tGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           <  2:3435273/140‑1 (MQ=255)

                                                                                                            tGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           <  2:397503/140‑1 (MQ=255)

                                                                                                            tGACCAACAGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           <  2:3435595/140‑1 (MQ=255)

                                                                                                                    aGCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTc                   >  2:5261108/1‑140 (MQ=255)

                                                                                                                     gCGGTTGATTGATCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCa                  <  2:1389850/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAGAGTTAATCTTTTCAACAg              <  2:4884056/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:1303661/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:1619884/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:5534100/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:2200024/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  1:3403159/140‑1 (MQ=255)

                                                                                                                         ttgattgaTCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:290018/140‑1 (MQ=255)

                                                                                                                          tgattgatCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  1:2117088/140‑1 (MQ=255)

                                                                                                                          tgattgatCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  2:672614/140‑1 (MQ=255)

                                                                                                                              tgatCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTc         <  1:5023474/140‑1 (MQ=255)

                                                                                                                                atCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       >  1:3394610/1‑140 (MQ=255)

                                                                                                                                atCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       >  1:3394071/1‑140 (MQ=255)

                                                                                                                                atCAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACCTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAt       >  1:3125206/1‑140 (MQ=255)

                                                                                                                                  cAGGTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaa     >  2:2445099/1‑140 (MQ=255)

                                                                                                                                     gTAGAGGGGG‑CGCTGTCCGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCGGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGGTAATCTTTTCAACAGCTGTCATAGAGt  >  1:4528012/1‑140 (MQ=255)

                                                                                                                                     gTAGAGGGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGt  >  1:5012733/1‑140 (MQ=255)

GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGG‑CGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400457‑400728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACT  >  NZ_CP025268/400452‑400744

GCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGC                                                                                                                                                 >  SRR14920167.4508110/1‑151 (MQ=60)

   GAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGT                                                                                                                                              >  SRR14920167.1336359/1‑151 (MQ=60)

     GTTGTTATTTAAGCTTGCCCAAAAAGAAGACGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTATAGGGGGCGCTGTAC                                                                                                                                            >  SRR14920167.668237/1‑151 (MQ=60)

     GTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTAC                                                                                                                                            <  SRR14920167.2433336/151‑1 (MQ=60)

        GTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAG                                                                                                                                         >  SRR14920167.277176/1‑151 (MQ=60)

         TTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGG                                                                                                                                        <  SRR14920167.544011/151‑1 (MQ=60)

         TTATTCAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGG                                                                                                                                        <  SRR14920167.3781940/151‑1 (MQ=60)

          TATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGT                                                                                                                                       <  SRR14920167.3859722/151‑1 (MQ=60)

          TATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGT                                                                                                                                       <  SRR14920167.1815643/151‑1 (MQ=60)

          TATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGT                                                                                                                                       <  SRR14920167.869019/151‑1 (MQ=60)

           ATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTA                                                                                                                                      >  SRR14920167.3960097/1‑151 (MQ=60)

           ATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTA                                                                                                                                      >  SRR14920167.1410940/1‑151 (MQ=60)

           ATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTA                                                                                                                                      >  SRR14920167.895692/1‑151 (MQ=60)

           ATTTAAGCTTGCCCAAAAAGAAAAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAACAGGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTA                                                                                                                                      >  SRR14920167.4649721/1‑151 (MQ=60)

                AGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCC                                                                                                                                 <  SRR14920167.3593292/151‑1 (MQ=60)

                 GCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCC                                                                                                                                <  SRR14920167.2685081/151‑1 (MQ=60)

                            AAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCAT                                                                                                                     >  SRR14920167.3211162/1‑151 (MQ=60)

                              GAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTC                                                                                                                   >  SRR14920167.2543776/1‑151 (MQ=60)

                               AAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCCATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCC                                                                                                                  <  SRR14920167.4669852/151‑1 (MQ=60)

                                    GAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACG                                                                                                             >  SRR14920167.3651953/1‑151 (MQ=60)

                                    GAGTAGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGACATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACG                                                                                                             <  SRR14920167.2337590/151‑1 (MQ=60)

                                      GTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGAC                                                                                                           <  SRR14920167.1188113/151‑1 (MQ=60)

                                      GTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGAC                                                                                                           <  SRR14920167.4343266/151‑1 (MQ=60)

                                      GTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGAC                                                                                                           <  SRR14920167.4343227/151‑1 (MQ=60)

                                      GTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGAC                                                                                                           <  SRR14920167.4343160/151‑1 (MQ=60)

                                          AAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATA                                                                                                       >  SRR14920167.5340636/1‑151 (MQ=60)

                                                CTGTGTGCGCAGGTAGAAGGTTTGGAGATTATCGTCACTGCCATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGC                                                                                                 <  SRR14920167.536563/151‑1 (MQ=60)

                                                CTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGC                                                                                                 <  SRR14920167.1764883/151‑1 (MQ=60)

                                                CTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGC                                                                                                 <  SRR14920167.536690/151‑1 (MQ=60)

                                                  GTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTG                                                                                               >  SRR14920167.984677/1‑151 (MQ=60)

                                                    GTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCT                                                                                             >  SRR14920167.200262/1‑151 (MQ=60)

                                                     TGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTG                                                                                            <  SRR14920167.4738669/151‑1 (MQ=60)

                                                          AGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGA                                                                                       <  SRR14920167.1386369/151‑1 (MQ=60)

                                                          AGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTGGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGA                                                                                       <  SRR14920167.4189689/151‑1 (MQ=60)

                                                           GGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATTAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCGGCGCGAT                                                                                      >  SRR14920167.4437875/1‑151 (MQ=60)

                                                           GGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCTTGACGACGATACGGAGCTGCTGCGCGAT                                                                                      >  SRR14920167.2093503/1‑151 (MQ=60)

                                                           GGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAT                                                                                      >  SRR14920167.860718/1‑151 (MQ=60)

                                                           GGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAT                                                                                      >  SRR14920167.2013192/1‑151 (MQ=60)

                                                           GGTAGAAGCTTTGGAGAATATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATGAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCGGCGCGAT                                                                                      >  SRR14920167.4438259/1‑151 (MQ=60)

                                                                  GCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAA                                                                               <  SRR14920167.3084909/151‑1 (MQ=60)

                                                                       GGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAG                                                                          <  SRR14920167.2735674/151‑1 (MQ=60)

                                                                                CGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAG                                                                 <  SRR14920167.5365259/151‑1 (MQ=60)

                                                                                CGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAG                                                                 <  SRR14920167.2150698/151‑1 (MQ=60)

                                                                                  TCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCA                                                               >  SRR14920167.1624651/1‑151 (MQ=60)

                                                                                     CTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCC                                                            >  SRR14920167.2712829/1‑151 (MQ=60)

                                                                                          gtctcgtgggctcggagatgtgtataagagacagGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTC                                                       <  SRR14920167.3255791/117‑1 (MQ=60)

                                                                                             CTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGT                                                    <  SRR14920167.5178230/151‑1 (MQ=60)

                                                                                               TCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAA                                                  >  SRR14920167.668193/1‑151 (MQ=60)

                                                                                               TCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAA                                                  >  SRR14920167.37317/1‑151 (MQ=60)

                                                                                                    ATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGT                                             <  SRR14920167.4637491/151‑1 (MQ=60)

                                                                                                    ATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGT                                             <  SRR14920167.2608842/151‑1 (MQ=60)

                                                                                                         GCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATC                                        <  SRR14920167.4186783/151‑1 (MQ=60)

                                                                                                                          GCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAT                       >  SRR14920167.3401608/1‑151 (MQ=60)

                                                                                                                          GCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAT                       >  SRR14920167.3402149/1‑151 (MQ=60)

                                                                                                                          GCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACCTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCAT                       >  SRR14920167.3131988/1‑151 (MQ=60)

                                                                                                                              TTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAG                   <  SRR14920167.1306314/151‑1 (MQ=60)

                                                                                                                              TTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAG                   <  SRR14920167.1623191/151‑1 (MQ=60)

                                                                                                                              TTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAG                   <  SRR14920167.2204690/151‑1 (MQ=60)

                                                                                                                              TTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAG                   <  SRR14920167.3410708/151‑1 (MQ=60)

                                                                                                                               TGATTGATCAGGTAGAGGGGGCGCTGTCCGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCGGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGGTAATCTTTTCAACAGCTGTCATAGAGT                  >  SRR14920167.4538186/1‑151 (MQ=60)

                                                                                                                               TGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT                  >  SRR14920167.5024230/1‑151 (MQ=60)

                                                                                                                               TGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT                  <  SRR14920167.2121544/151‑1 (MQ=60)

                                                                                                                                   TGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTC              <  SRR14920167.5034981/151‑1 (MQ=60)

                                                                                                                                     ATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCAC            >  SRR14920167.302411/1‑151 (MQ=60)

                                                                                                                                     ATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCAC            >  SRR14920167.1839954/1‑151 (MQ=60)

                                                                                                                                         GGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTAACTGCC        >  SRR14920167.2590560/1‑151 (MQ=60)

                                                                                                                                          GTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCG       >  SRR14920167.4593424/1‑151 (MQ=60)

                                                                                                                                              G‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTTACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACT  <  SRR14920167.5150667/151‑1 (MQ=60)

                                                                                                                                              G‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTCACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACT  <  SRR14920167.5150791/151‑1 (MQ=60)

                                                                                                                                              G‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTCACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACT  <  SRR14920167.5150440/151‑1 (MQ=60)

                                                                                                                                              G‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTCACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACT  <  SRR14920167.5150077/151‑1 (MQ=60)

GCTGAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGTTGTCACGGCCGAGACT  >  NZ_CP025268/400452‑400744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`