Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R2 870 341.9 11209456 97.2% 10895591 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,109,323 C→T E107E (GAG→GAA)  csgE ← curli production assembly/transport protein CsgE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,109,3230CT99.6% 842.0 / NA 228E107E (GAG→GAA) csgEcurli production assembly/transport protein CsgE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (102/125);  minor base A (1/0);  total (103/125)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

CGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCT  >  NZ_CP025268/1109188‑1109454
                                                                                                                                       |                                                                                                                                   
cGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGcttctt                                                                                                                                 <  2:1797055/140‑1 (MQ=255)
cGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGcttctt                                                                                                                                 >  2:3717204/1‑140 (MQ=255)
cGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTACGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGcttctt                                                                                                                                 <  2:4374152/140‑1 (MQ=255)
  aaaTAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa                                                                                                                               <  1:110331/140‑1 (MQ=255)
     tAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGtt                                                                                                                            <  1:64977/140‑1 (MQ=255)
          aggAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAAGTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  1:2948840/138‑1 (MQ=255)
          aTGAGTAGACCTACTGCATGTTTGACACCCATTGCAGCCCCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  1:2658145/140‑1 (MQ=255)
          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGACTTCATCATGCGCCCAATCGCCCGTACTTCATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  2:2035077/140‑1 (MQ=255)
          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  2:545217/140‑1 (MQ=255)
          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  2:756667/140‑1 (MQ=255)
          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  2:2260831/140‑1 (MQ=255)
          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       <  2:2827839/140‑1 (MQ=255)
          aTGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAt                                                                                                                       >  1:156256/1‑140 (MQ=255)
           tGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATc                                                                                                                      <  1:4686082/140‑1 (MQ=255)
           tGAGCAGAACTACTGCATGTTTGACACGCATTGCCGCCTCCGAACAATTTTTTATTTAGACTTCATCATGCGCCAAATCGCCCGTCCTTCATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATc                                                                                                                      <  2:1544865/140‑1 (MQ=255)
             ggTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAg                                                                                                                    >  1:721261/2‑140 (MQ=255)
             aGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAg                                                                                                                    >  2:3963269/1‑140 (MQ=255)
                       ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacg                                                                                                          >  1:2566976/1‑140 (MQ=255)
                       ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacg                                                                                                          >  1:4887886/1‑140 (MQ=255)
                       ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacg                                                                                                          >  2:2586403/1‑140 (MQ=255)
                       ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTGATAGTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCGGATTTAGCTTGCGACGATATAGGGCTTCTTCAGATCGAATGAGTGCAAAgacg                                                                                                          >  2:1239543/1‑140 (MQ=255)
                        tGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacga                                                                                                         >  1:2552630/1‑140 (MQ=255)
                         gCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacgac                                                                                                        >  2:5284226/1‑140 (MQ=255)
                         gCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacgac                                                                                                        >  2:5569434/1‑140 (MQ=255)
                         gCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAgacgac                                                                                                        >  2:1627118/1‑140 (MQ=255)
                          cgtGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACCCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAGCAGTGCAAAGACGACa                                                                                                       >  1:4473505/3‑140 (MQ=255)
                           aTGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAg                                                                                                      <  1:1352448/140‑1 (MQ=255)
                               ttGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGtttt                                                                                                  >  1:2761177/1‑140 (MQ=255)
                               ttGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGtttt                                                                                                  >  2:356413/1‑140 (MQ=255)
                               ttGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGcttt                                                                                                  >  2:4233388/1‑140 (MQ=255)
                                tGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTtc                                                                                                 >  1:922829/1‑140 (MQ=255)
                                 gACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATATATCTGGCGACGACGTAGTGCTTCTTCAGTTTGAACAAGTGCACAGACGACacatttgt                                                                                                >  2:5191681/1‑133 (MQ=255)
                                    acGCCTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa                                                                                             <  1:835486/140‑1 (MQ=255)
                                    acGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa                                                                                             <  2:199040/140‑1 (MQ=255)
                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                          <  2:296442/140‑1 (MQ=255)
                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                          <  1:2722787/140‑1 (MQ=255)
                                        aTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATGTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCAAAGtc                                                                                         >  2:789392/1‑140 (MQ=255)
                                          tGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGtctc                                                                                       >  2:1869106/1‑140 (MQ=255)
                                            cAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCtt                                                                                     >  1:1930999/1‑140 (MQ=255)
                                                 tCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCaa                                                                                >  1:5239287/1‑140 (MQ=255)
                                                  ccGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                               <  1:1179972/140‑1 (MQ=255)
                                                  ccGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                               >  1:4288622/1‑140 (MQ=255)
                                                  ccGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                               >  1:2415824/1‑140 (MQ=255)
                                                    gAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACgg                                                                             <  2:2347637/140‑1 (MQ=255)
                                                    gAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACgg                                                                             <  2:4615359/140‑1 (MQ=255)
                                                      aCAATTTTTTATTTAGAATTCATCATGCACCAAATCGCCCGTACTTAATAACGCCTGATTTGTCTGGCGACAATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGaa                                                                           >  2:5146109/1‑140 (MQ=255)
                                                         aTTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATa                                                                        >  2:3662704/1‑140 (MQ=255)
                                                          ttttttATTTAGAATTCATCATGCGCCAAATCGCCTGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATaa                                                                       >  2:263731/1‑140 (MQ=255)
                                                          ttttttATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCATTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATaa                                                                       >  1:2813471/1‑140 (MQ=255)
                                                                atttaGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGt                                                                 <  1:4195768/140‑1 (MQ=255)
                                                                atttaGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGt                                                                 <  1:2865543/140‑1 (MQ=255)
                                                                 tttaGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTc                                                                <  1:2159131/140‑1 (MQ=255)
                                                                   taGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTg                                                              <  1:3404891/140‑1 (MQ=255)
                                                                     gAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGa                                                            <  2:1568678/140‑1 (MQ=255)
                                                                         tcatcaTGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAAt                                                        <  2:389417/140‑1 (MQ=255)
                                                                               tGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTc                                                  <  1:609823/140‑1 (MQ=255)
                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTcc                                                 >  2:3834371/1‑140 (MQ=255)
                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                            <  1:382420/140‑1 (MQ=255)
                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                            <  2:1328831/140‑1 (MQ=255)
                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                            >  2:2163368/1‑140 (MQ=255)
                                                                                     aaaTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                            <  1:756112/140‑1 (MQ=255)
                                                                                       aTCGGCTGTACTTTATAACGCCTAATTTATCGGGCGACGATTTGGTGCTTCTTTAGTTTGAATCAGTGCCAAGACGACAGTTTTATCGAAATCTCTTTTCAACGGAAATAAAAAAGTCTGGAGAATAACGTGCTGATTGa                                          <  2:3585122/140‑1 (MQ=255)
                                                                                       aTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                          <  1:2399089/140‑1 (MQ=255)
                                                                                       aTCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                          <  1:1041690/140‑1 (MQ=255)
                                                                                        tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                         <  1:5471997/140‑1 (MQ=255)
                                                                                        tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                         <  1:1136829/140‑1 (MQ=255)
                                                                                                aCTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                          <  1:3890984/131‑1 (MQ=255)
                                                                                                   taataaCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGa                              <  1:4460327/140‑1 (MQ=255)
                                                                                                    aataaCGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGCAAAATAACGTCCTGATTGACCGTTATAGTGAt                             >  2:2756638/1‑140 (MQ=255)
                                                                                                       aaCGCCTGATTTATCTGGAGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCACAGACCACAGTATTCTCGAAGTCTCTTTTCAACCGAAATACAAAAGTATGGAAAATAACGTCCTGATTGACCGTTATAGTGACCCa                          <  2:3123807/140‑1 (MQ=255)
                                                                                                        aCGACTGATCTATCTGGCGACGACTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGCCGACAGTTCTCTCGAAGTCTCTTCTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAg                         <  2:4281567/140‑1 (MQ=255)
                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                        >  1:3310633/1‑140 (MQ=255)
                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                        >  1:356520/1‑140 (MQ=255)
                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                        >  1:4151806/1‑140 (MQ=255)
                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTGTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGc                        >  1:27898/1‑140 (MQ=255)
                                                                                                         cGCCTGATTTATCTGGAGACGAATTAGTGCTTCTTAAGTTTGAGTCAGTGCAAAGACGACAGATGTTTCGAAGTCTCTTATCAACGGAAATAAAAAAGTCGGGAAAATAACGTCCTGATGAAGGGTTATAGTGATGCAGc                        >  2:4081278/1‑140 (MQ=255)
                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt                      >  1:2443730/1‑140 (MQ=255)
                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt                      >  2:1162263/1‑140 (MQ=255)
                                                                                                            cTGATTTATCTGGCGACGATTTAGTGGTTCTTCAGTTTGAATGAGTGCAAAGACGACAGTGTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCttt                     >  2:3255631/1‑139 (MQ=255)
                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTc                     >  2:4834571/1‑140 (MQ=255)
                                                                                                             ttatgtataTGACGACGATTTAGTGCTTCTTCCGTTTCACTCAGAGCAACGACGACAGTTTTCTCGAAGTCTCTTTTCAACCGAACTACAAAAGTCTGCAGAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                    <  2:1207327/135‑1 (MQ=255)
                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                    <  2:2470825/140‑1 (MQ=255)
                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                    <  2:2415824/140‑1 (MQ=255)
                                                                                                             tGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                    <  1:3963269/140‑1 (MQ=255)
                                                                                                              gATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTccc                   >  1:3724899/1‑140 (MQ=255)
                                                                                                              gATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTccc                   >  1:5408211/1‑140 (MQ=255)
                                                                                                              gATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTccc                   >  1:3205712/1‑140 (MQ=255)
                                                                                                                  tATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATc               <  2:5098736/140‑1 (MQ=255)
                                                                                                                    tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTGCCCATCGt             >  1:215994/1‑140 (MQ=255)
                                                                                                                    gcTGGCGACGATTAAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCACCATCGt             >  2:2872103/2‑140 (MQ=255)
                                                                                                                     cTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTg            >  1:3621691/1‑140 (MQ=255)
                                                                                                                       ggCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACAACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGCCCGTTATAGTGATCCAGCTTCCCCATCGTGCa          <  2:2767537/140‑1 (MQ=255)
                                                                                                                         cgacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACt        <  1:5083639/140‑1 (MQ=255)
                                                                                                                         cgacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACt        <  1:1668338/140‑1 (MQ=255)
                                                                                                                         cgacgaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACt        >  1:87452/1‑140 (MQ=255)
                                                                                                                         cgacgaTATAGTGCTTCCTGAGTTTGAATCAGTGCAAAGACGACAGGTTTATGGAAGTCTCTTTTCAACGGAAAGAGAAAAGCCTGGAAAAGAACGGCCTGACAGACCGTTATAGTGATGCAGCTGCTCCATCGTGCACt        >  2:4860302/1‑140 (MQ=255)
                                                                                                                             gaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAGAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGc    <  1:3686373/140‑1 (MQ=255)
                                                                                                                             gaTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGc    <  2:2114273/140‑1 (MQ=255)
                                                                                                                              aTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                            <  1:5252277/99‑1 (MQ=255)
                                                                                                                              aTTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGcc   >  2:673285/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCT  >  NZ_CP025268/1109188‑1109454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTT  >  NZ_CP025268/1109173‑1109472
                                                                                                                                                      |                                                                                                                                                     
AGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCT                                                                                                                                                       >  SRR14920167.2118714/1‑151 (MQ=60)
  CCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTC                                                                                                                                                     <  SRR14920167.2387368/151‑1 (MQ=60)
  CCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTC                                                                                                                                                     <  SRR14920167.5248462/151‑1 (MQ=60)
     AACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTC                                                                                                                                                  <  SRR14920167.4222312/151‑1 (MQ=60)
         TAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTT                                                                                                                                              <  SRR14920167.3936811/151‑1 (MQ=60)
          AATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTT                                                                                                                                             <  SRR14920167.4747394/151‑1 (MQ=60)
              GCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAT                                                                                                                                         >  SRR14920167.156670/1‑151 (MQ=60)
                 AAATAAGCATGGGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAG                                                                                                                                      >  SRR14920167.722897/1‑151 (MQ=60)
                 AAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAG                                                                                                                                      <  SRR14920167.110640/151‑1 (MQ=60)
                 AAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAG                                                                                                                                      <  SRR14920167.112338/151‑1 (MQ=60)
                 AAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAG                                                                                                                                      >  SRR14920167.5198742/1‑151 (MQ=60)
                    TAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGC                                                                                                                                   <  SRR14920167.65176/151‑1 (MQ=60)
                    TAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGC                                                                                                                                   <  SRR14920167.1374213/151‑1 (MQ=60)
                         ATGAGTAGACCTACTGCATGTTTGACACCCATTGCAGCCCCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGA                                                                                                                              <  SRR14920167.2663616/151‑1 (MQ=60)
                         ATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGA                                                                                                                              <  SRR14920167.86695/151‑1 (MQ=60)
                         ATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGA                                                                                                                              <  SRR14920167.349461/151‑1 (MQ=60)
                         ATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGA                                                                                                                              <  SRR14920167.654887/151‑1 (MQ=60)
                         AGGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAAGTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGA                                                                                                                              <  SRR14920167.2954940/151‑1 (MQ=60)
                          TGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGAC                                                                                                                             <  SRR14920167.4696729/151‑1 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.2089142/1‑151 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.2735080/1‑151 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.2572265/1‑151 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.2572071/1‑151 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.632189/1‑151 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.793887/1‑151 (MQ=60)
                           GAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACG                                                                                                                            >  SRR14920167.4898990/1‑151 (MQ=60)
                            AGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGA                                                                                                                           >  SRR14920167.2557896/1‑151 (MQ=60)
                              TAGAACTACTGCGTGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACCCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAAGCAGTGCAAAGACGACA                                                                                                                         >  SRR14920167.4483548/1‑151 (MQ=60)
                              TAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACA                                                                                                                         >  SRR14920167.4458258/1‑151 (MQ=60)
                                GAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGT                                                                                                                       >  SRR14920167.913485/1‑151 (MQ=60)
                                GAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGT                                                                                                                       <  SRR14920167.2491275/151‑1 (MQ=60)
                                   CTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTT                                                                                                                    >  SRR14920167.2766866/1‑151 (MQ=60)
                                    TACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTC                                                                                                                   >  SRR14920167.924792/1‑151 (MQ=60)
                                          ATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAG                                                                                                             <  SRR14920167.4014988/151‑1 (MQ=60)
                                          ATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAG                                                                                                             <  SRR14920167.1355183/151‑1 (MQ=60)
                                             TTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCTACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCT                                                                                                          >  SRR14920167.4656144/1‑151 (MQ=60)
                                             TTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCCACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCT                                                                                                          >  SRR14920167.4656107/1‑151 (MQ=60)
                                                GACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTT                                                                                                       >  SRR14920167.1934996/1‑151 (MQ=60)
                                                   ACGCCTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTC                                                                                                    <  SRR14920167.837316/151‑1 (MQ=60)
                                                     GCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAA                                                                                                  >  SRR14920167.5251234/1‑151 (MQ=60)
                                                     GCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAA                                                                                                  >  SRR14920167.5251190/1‑151 (MQ=60)
                                                      CATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAC                                                                                                 >  SRR14920167.2420840/1‑151 (MQ=60)
                                                      CATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAC                                                                                                 <  SRR14920167.2728408/151‑1 (MQ=60)
                                                      CATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAC                                                                                                 >  SRR14920167.4298207/1‑151 (MQ=60)
                                                              CCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCATTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAA                                                                                         >  SRR14920167.2819279/1‑151 (MQ=60)
                                                                TCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAA                                                                                       <  SRR14920167.2437619/151‑1 (MQ=60)
                                                                 CCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAA                                                                                      <  SRR14920167.5425694/151‑1 (MQ=60)
                                                                 CCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAA                                                                                      <  SRR14920167.5426856/151‑1 (MQ=60)
                                                                 CCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAA                                                                                      <  SRR14920167.1182363/151‑1 (MQ=60)
                                                                               ATTTAGAATTCATCATGCGCCACATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAA                                                                        <  SRR14920167.3725253/151‑1 (MQ=60)
                                                                               ATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAA                                                                        <  SRR14920167.2871439/151‑1 (MQ=60)
                                                                               ATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAA                                                                        <  SRR14920167.264783/151‑1 (MQ=60)
                                                                               ATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAA                                                                        <  SRR14920167.4205108/151‑1 (MQ=60)
                                                                                TTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAAC                                                                       <  SRR14920167.2801467/151‑1 (MQ=60)
                                                                                TTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAAC                                                                       <  SRR14920167.2164005/151‑1 (MQ=60)
                                                                                TTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAAC                                                                       <  SRR14920167.2163685/151‑1 (MQ=60)
                                                                                TTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAAC                                                                       <  SRR14920167.374025/151‑1 (MQ=60)
                                                                                  TAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGT                                                                     <  SRR14920167.3412443/151‑1 (MQ=60)
                                                                                    GAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCC                                                                   >  SRR14920167.3693437/1‑151 (MQ=60)
                                                                                       TTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGA                                                                >  SRR14920167.3298689/1‑151 (MQ=60)
                                                                                       TTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGA                                                                >  SRR14920167.860227/1‑151 (MQ=60)
                                                                                        TCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAT                                                               <  SRR14920167.686664/151‑1 (MQ=60)
                                                                                         CATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATT                                                              >  SRR14920167.747431/1‑151 (MQ=60)
                                                                                         CATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATT                                                              >  SRR14920167.1076964/1‑151 (MQ=60)
                                                                                              TGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCG                                                         <  SRR14920167.611210/151‑1 (MQ=60)
                                                                                                 GCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTA                                                      >  SRR14920167.3710108/1‑151 (MQ=60)
                                                                                                    cgtgggctcggagatgtgtataagagacagATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.5264202/121‑1 (MQ=60)
                                                                                                    AAATCGGCCGTACTTCATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGCAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.3875674/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTACTAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.3099604/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTACTAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTGTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTATTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.3119979/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.5581823/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.2940920/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.383336/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.4203166/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.1760915/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.2707417/151‑1 (MQ=60)
                                                                                                    AAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAG                                                   <  SRR14920167.757809/151‑1 (MQ=60)
                                                                                                      ATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTG                                                 <  SRR14920167.2404073/151‑1 (MQ=60)
                                                                                                      ATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTG                                                 <  SRR14920167.4206625/151‑1 (MQ=60)
                                                                                                      ATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTG                                                 <  SRR14920167.357283/151‑1 (MQ=60)
                                                                                                      ATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTG                                                 <  SRR14920167.1043827/151‑1 (MQ=60)
                                                                                                      ATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTGTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTG                                                 <  SRR14920167.3899282/151‑1 (MQ=60)
                                                                                                      ATCGCCCGTACTTAAGAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTG                                                 <  SRR14920167.5035945/151‑1 (MQ=60)
                                                                                                       TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGA                                                <  SRR14920167.1139143/151‑1 (MQ=60)
                                                                                                       TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGA                                                <  SRR14920167.4810797/151‑1 (MQ=60)
                                                                                                       TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGA                                                <  SRR14920167.5484257/151‑1 (MQ=60)
                                                                                                             GTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGC                                          >  SRR14920167.2505697/1‑151 (MQ=60)
                                                                                                             GTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGC                                          >  SRR14920167.4161078/1‑151 (MQ=60)
                                                                                                             GTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGC                                          >  SRR14920167.3317777/1‑151 (MQ=60)
                                                                                                             GTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGC                                          >  SRR14920167.357390/1‑151 (MQ=60)
                                                                                                             GTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGC                                          >  SRR14920167.2867097/1‑151 (MQ=60)
                                                                                                             GTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTGTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGC                                          >  SRR14920167.27996/1‑151 (MQ=60)
                                                                                                              TACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCT                                         >  SRR14920167.951633/1‑151 (MQ=60)
                                                                                                              TACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCT                                         >  SRR14920167.3589201/1‑151 (MQ=60)
                                                                                                               ACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTT                                        >  SRR14920167.4208338/1‑151 (MQ=60)
                                                                                                               ACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTT                                        >  SRR14920167.2448797/1‑151 (MQ=60)
                                                                                                                CTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTC                                       <  SRR14920167.1146489/151‑1 (MQ=60)
                                                                                                                CTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTC                                       >  SRR14920167.2792663/1‑151 (MQ=60)
                                                                                                                  TAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCC                                     <  SRR14920167.4470856/151‑1 (MQ=60)
                                                                                                                  TAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCC                                     <  SRR14920167.4470340/151‑1 (MQ=60)
                                                                                                                  TAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCC                                     >  SRR14920167.3212675/1‑151 (MQ=60)
                                                                                                                  TAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCC                                     >  SRR14920167.3732962/1‑151 (MQ=60)
                                                                                                                  TAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCC                                     >  SRR14920167.5420384/1‑151 (MQ=60)
                                                                                                                   AATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCC                                    <  SRR14920167.2408003/151‑1 (MQ=60)
                                                                                                                    ATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCA                                   >  SRR14920167.2257549/1‑151 (MQ=60)
                                                                                                                     TAACGCCTGAGTGATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAATTCTCTTTTCAACGGAAATAAAAAAGTATGGAAAATAACGTCCTGATTGACCGTTATAGTAATCCAGCTTCCCCAT                                  <  SRR14920167.4088485/151‑1 (MQ=60)
                                                                                                                        CGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTGCCCATCGT                               >  SRR14920167.216536/1‑151 (MQ=60)
                                                                                                                         GCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTG                              >  SRR14920167.3629579/1‑151 (MQ=60)
                                                                                                                            TGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCAC                           <  SRR14920167.1245778/151‑1 (MQ=60)
                                                                                                                            TGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCAC                           <  SRR14920167.3349959/151‑1 (MQ=60)
                                                                                                                            TGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCAC                           <  SRR14920167.3971688/151‑1 (MQ=60)
                                                                                                                            TGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCAC                           <  SRR14920167.3971632/151‑1 (MQ=60)
                                                                                                                             GATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACT                          <  SRR14920167.226089/151‑1 (MQ=60)
                                                                                                                             GATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACT                          >  SRR14920167.87704/1‑151 (MQ=60)
                                                                                                                              ATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTG                         >  SRR14920167.2815899/1‑151 (MQ=60)
                                                                                                                               TTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGG                        >  SRR14920167.3922366/1‑151 (MQ=60)
                                                                                                                                 TATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGC                      <  SRR14920167.1323852/151‑1 (MQ=60)
                                                                                                                                      GGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTC                 <  SRR14920167.1629892/151‑1 (MQ=60)
                                                                                                                                      GGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTC                 <  SRR14920167.5375143/151‑1 (MQ=60)
                                                                                                                                      GGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTC                 <  SRR14920167.2722443/151‑1 (MQ=60)
                                                                                                                                        CGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACGGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCAT               >  SRR14920167.5118299/1‑151 (MQ=60)
                                                                                                                                        CGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCAT               <  SRR14920167.5095272/151‑1 (MQ=60)
                                                                                                                                        CGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCAT               <  SRR14920167.1671754/151‑1 (MQ=60)
                                                                                                                                            GATTTAGTGCTTCTTCAGTTTGAATCAGTGCAGAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAAT           <  SRR14920167.3694361/151‑1 (MQ=60)
                                                                                                                                            GATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTGTAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAAT           >  SRR14920167.5114713/1‑151 (MQ=60)
                                                                                                                                            GATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAAT           >  SRR14920167.62628/1‑151 (MQ=60)
                                                                                                                                            GATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAAT           >  SRR14920167.5228917/1‑151 (MQ=60)
                                                                                                                                             ATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATC          >  SRR14920167.2332303/1‑151 (MQ=60)
                                                                                                                                             ATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATC          >  SRR14920167.5319163/1‑151 (MQ=60)
                                                                                                                                             ATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATC          >  SRR14920167.5312557/1‑151 (MQ=60)
                                                                                                                                             ATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATC          >  SRR14920167.4469865/1‑151 (MQ=60)
                                                                                                                                             ATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATC          >  SRR14920167.3169852/1‑151 (MQ=60)
                                                                                                                                             ATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACACTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATC          <  SRR14920167.5150028/151‑1 (MQ=60)
                                                                                                                                              TTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCG         <  SRR14920167.1793217/151‑1 (MQ=60)
                                                                                                                                                    GCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGT   <  SRR14920167.4639660/151‑1 (MQ=60)
                                                                                                                                                     CTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTT  <  SRR14920167.3207462/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                     
AGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTT  >  NZ_CP025268/1109173‑1109472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑