Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R3 779 308.9 10220750 96.7% 9883465 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,411,091 C→T P43S (CCT→TCT)  dusB → tRNA dihydrouridine synthase DusB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,411,0910CT98.0% 843.2 / NA 245P43S (CCT→TCT) dusBtRNA dihydrouridine synthase DusB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (152/87);  minor base G (5/0);  total (158/87)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG  >  NZ_CP025268/3410954‑3411226
                                                                                                                                         |                                                                                                                                       
tttCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCt                                                                                                                                       >  1:2898982/1‑140 (MQ=255)
tttCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCt                                                                                                                                       >  1:3210931/1‑140 (MQ=255)
tttCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCt                                                                                                                                       >  1:2566420/1‑140 (MQ=255)
tttCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCt                                                                                                                                       >  1:2956975/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGTCAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTc                                                                                                                                     >  2:4763059/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGACAATGTGAGCTCAGAAATCGCCTGATCGCAGCGGCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCGACGAGATGGGAGCCGGTGTGACAGTATCCGAGATGGTGTCTTc                                                                                                                                     >  2:52520/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTc                                                                                                                                     >  1:4346340/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTc                                                                                                                                     >  1:3653092/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTc                                                                                                                                     >  1:478179/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTc                                                                                                                                     >  1:3322154/1‑140 (MQ=255)
  tCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATAATGTCTTc                                                                                                                                     >  2:4272602/1‑140 (MQ=255)
     tAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTaa                                                                                                                                  <  2:2385694/140‑1 (MQ=255)
      aaCTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAc                                                                                                                                 >  1:1817427/1‑140 (MQ=255)
      aaCTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAc                                                                                                                                 >  1:2348066/1‑140 (MQ=255)
      aaCTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAc                                                                                                                                 >  2:1535005/1‑140 (MQ=255)
      aaCTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATGGACAGTATCCGAGGTGATGTCTTCTAAc                                                                                                                                 >  1:4618182/1‑140 (MQ=255)
           aTGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCcaca                                                                                                                            >  1:3498190/1‑140 (MQ=255)
             gcgcATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAgg                                                                                                                          >  2:4937502/1‑140 (MQ=255)
                cATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGttt                                                                                                                       >  1:3542076/1‑140 (MQ=255)
                   cGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTggg                                                                                                                    >  2:1889193/1‑140 (MQ=255)
                     gagaaTATCAGCGCAGAAATCGCCTGATCGCAGCGCCCATGGCCGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGGGCCGGAGTGACAGTCTCCGAGATGATGGCTTCTAACCCACAGGTGTGGGaa                                                                                                                  >  2:3470930/4‑140 (MQ=255)
                               gCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                        >  2:436322/1‑140 (MQ=255)
                               gCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAt                                                                                                        >  1:1541759/1‑140 (MQ=255)
                               gCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGATTGGGAAAGCGGCAAAt                                                                                                        >  1:2647789/1‑140 (MQ=255)
                                cTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtc                                                                                                       >  2:2032019/1‑140 (MQ=255)
                                cTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtc                                                                                                       >  1:4605702/1‑140 (MQ=255)
                                  cAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAtctc                                                                                                     >  1:3253494/1‑140 (MQ=255)
                                    gAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGAATATGTCTTCTAACCCACAAGGTTGGGAAAGCAACAAATCTCgg                                                                                                   >  1:1283786/1‑139 (MQ=255)
                                    gAAACCGCCTGATCGCAGCGCCCATGACTGGCATTACAGACAGACCTCTTCGGACGTTGTGCCACGAGATGGGAGCCGCATTGCCAGTACCCGCGCTGATCCCTTCTAAGACACCGGTCTGGGCAAGCTACAAATCTCGt                                                                                                   >  2:4875222/1‑140 (MQ=255)
                                     aaaTCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGtt                                                                                                  >  1:4388548/1‑140 (MQ=255)
                                     aaaTCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTCTGGGAAAGCGACAAATCTCGtt                                                                                                  >  1:2455636/1‑140 (MQ=255)
                                      aaTCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCGACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGttt                                                                                                 <  2:1183654/140‑1 (MQ=255)
                                          gCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACgg                                                                                             >  2:1988815/1‑140 (MQ=255)
                                          gCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAGTGACAGTATCCGAGATGATGTCTTCTAAGCCACAGGTTTGGGAAGGCGACAAAGCTCGttgtagg                                                                                             >  1:135494/1‑135 (MQ=255)
                                               aTCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGt                                                                                        <  2:3184562/140‑1 (MQ=255)
                                               aTCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGt                                                                                        >  1:3774271/1‑140 (MQ=255)
                                               aTCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGt                                                                                        >  2:4321496/1‑140 (MQ=255)
                                                 cGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACgg                                                                                             >  2:224956/1‑133 (MQ=255)
                                                 cGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACgg                                                                                             <  1:224956/133‑1 (MQ=255)
                                                      cgcCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACAtt                                                                                 >  2:1779775/1‑140 (MQ=255)
                                                           aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATtgatga                                                                            <  1:56853/140‑1 (MQ=255)
                                                           aTGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAGTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATttatga                                                                            >  2:2110424/1‑140 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAACTCTCGTTTACGGATGGTGCACATTGATGAActc                                                                        <  2:2455676/140‑3 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:4739750/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:4388548/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:2080205/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:3774271/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:2768670/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:92605/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:336025/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:4193041/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:2598401/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:2528137/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCGCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:1918035/140‑1 (MQ=255)
                                                               ctggcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTAGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:4023876/140‑1 (MQ=255)
                                                               ctggcATGACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:4059205/140‑1 (MQ=255)
                                                               ctcgcATTACAGGCAGACCTTTTCGGAAGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACCGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:3750771/137‑1 (MQ=255)
                                                               ctcgcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  1:4371089/137‑1 (MQ=255)
                                                                  gcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGt                                                                     >  2:2943219/1‑140 (MQ=255)
                                                                  gcATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAACTCGTTTACGGATGGTGCACATTGATGAACCCGGt                                                                     >  1:2510596/1‑140 (MQ=255)
                                                                   cATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTa                                                                    >  2:3693853/1‑140 (MQ=255)
                                                                   cATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTa                                                                    >  2:286677/1‑140 (MQ=255)
                                                                             agacCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGt                                                          >  2:4588995/1‑140 (MQ=255)
                                                                             agacCTTTTCGGACGTTGTGCTACGAGAGGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATTAACCCGGTATTCGCACCGt                                                          >  2:1077781/1‑140 (MQ=255)
                                                                             agacCTTTTCGGACGTTGGGTTACGATCTGCGAGCCGGATTGTCAGCTGCCGAGCTGTTGTCTTCTAACCCACAGGTGTGTGAACCCGACAAATCCCGCTTACGGATCGTGCCCAGTTACGAACCCCGTCTCCGcggcca                                                          >  2:3252363/1‑135 (MQ=255)
                                                                               actttttCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCCTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGCTTACGGATGGTGCGCATTGATGAACCCGGTATTTGCACGGTGc                                                        >  1:4839051/4‑140 (MQ=255)
                                                                               acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCTACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAGCGTGc                                                        >  1:14984/1‑140 (MQ=255)
                                                                               acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACCGGTTTGGGAAGGCGACACATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGc                                                        >  2:544254/1‑140 (MQ=255)
                                                                               acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGc                                                        >  1:3401804/1‑140 (MQ=255)
                                                                               acCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGc                                                        >  2:2341753/1‑140 (MQ=255)
                                                                                ccTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCcac                                                                                                                             <  2:3564948/70‑1 (MQ=255)
                                                                                  ttttCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCaaa                                                     <  2:4067497/140‑1 (MQ=255)
                                                                                            ttGTGCTACGAGATGGGAGCCGGATTGACAGTAGCCGAGATGATGTCTTCTATCCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGAGGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAg                                           <  2:4073243/140‑1 (MQ=255)
                                                                                                gCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGAt                                       <  1:3712836/140‑1 (MQ=255)
                                                                                                     gagaTGGGAGCCGGCTGGACAGTATCCGAGATGATGTCGTCTAACCCACAGGTGTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCgaa                                  >  2:4448725/1‑140 (MQ=255)
                                                                                                     gagaTGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAAccc                                                                        <  2:1418354/102‑1 (MQ=255)
                                                                                                      agaTGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCgaaa                                 >  2:3826851/1‑140 (MQ=255)
                                                                                                         tGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCCCAGGTTTGGGAAAGCGACAAATCTCGTTTACGGAGGGTGCACACTGAGGAACGCGGTATTCGCACGGTGCAAAGTGTTGGTAGCGATCCggaagaa                              >  2:3972318/1‑140 (MQ=255)
                                                                                                         tGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCgaaagaa                              >  2:1717958/1‑140 (MQ=255)
                                                                                                           ggAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTCCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAAt                            >  1:2845896/1‑140 (MQ=255)
                                                                                                              gCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGc                         >  1:4429866/1‑140 (MQ=255)
                                                                                                                 ggATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGa                      >  1:2053130/1‑140 (MQ=255)
                                                                                                                   aTTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATg                    <  1:1020106/140‑1 (MQ=255)
                                                                                                                   aTTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATg                    <  1:3177427/140‑1 (MQ=255)
                                                                                                                      gACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcag                 >  1:1468554/1‑140 (MQ=255)
                                                                                                                       aCAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagc                <  2:787416/140‑1 (MQ=255)
                                                                                                                        cAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagca               <  2:271109/140‑1 (MQ=255)
                                                                                                                        cAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATgcagca               <  1:3517944/140‑1 (MQ=255)
                                                                                                                            aTCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTa           >  1:5076666/1‑140 (MQ=255)
                                                                                                                             tCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTAt          >  1:3873048/1‑140 (MQ=255)
                                                                                                                             tCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTAt          >  1:3953492/1‑140 (MQ=255)
                                                                                                                               cGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCAAATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTa        <  2:2153704/140‑1 (MQ=255)
                                                                                                                                 cgatgatGTCTTATAACCCCCACGTTCGGGACATCGACCAATCCCGTTTACGGATGGAGCACATTGATGAACCCGGTATGCGCACCGTGCAAAATACGGCTAACGATCCGAAAGAAATGGAAGATGCGGCACGTATTCAc      >  2:4584549/2‑140 (MQ=255)
                                                                                                                                 agaTGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAAc      >  2:609322/1‑140 (MQ=255)
                                                                                                                                     gatgTCTTCTAACCCACCGGTTTGGGAAAGCGACAAATCTCGTTTCCGTATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTgg  >  1:3824963/1‑140 (MQ=255)
                                                                                                                                     gatgTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTAtt         <  2:3037244/133‑1 (MQ=255)
                                                                                                                                     gatgTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTAtt         >  1:3037244/1‑133 (MQ=255)
                                                                                                                                     gatgTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTgg  >  1:2382838/1‑140 (MQ=255)
                                                                                                                                     gatgTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTgg  >  1:172077/1‑140 (MQ=255)
                                                                                                                                     gatgTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAACGATCCGAAAGAAATGTCAGATGCAGCACGTATTAACGTgg  >  1:1638879/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG  >  NZ_CP025268/3410954‑3411226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGA  >  NZ_CP025268/3410943‑3411241
                                                                                                                                                    |                                                                                                                                                      
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.2905164/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.3217856/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.4733466/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.2571615/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.4405539/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.2520559/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.2963260/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGGCT                                                                                                                                                      >  SRR14920166.532366/1‑151 (MQ=60)
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGACGGATTGACAGTATCCGAGATGATGTCT                                                                                                                                                      >  SRR14920166.1627316/1‑151 (MQ=60)
  GTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTC                                                                                                                                                    >  SRR14920166.3329269/1‑151 (MQ=60)
  GTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTC                                                                                                                                                    >  SRR14920166.479273/1‑151 (MQ=60)
  GTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTC                                                                                                                                                    >  SRR14920166.3660903/1‑151 (MQ=60)
  GTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTC                                                                                                                                                    >  SRR14920166.4355827/1‑151 (MQ=60)
  GTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTC                                                                                                                                                    >  SRR14920166.4270546/1‑151 (MQ=60)
  GTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTC                                                                                                                                                    >  SRR14920166.2564194/1‑151 (MQ=60)
    ATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTA                                                                                                                                                  >  SRR14920166.2372701/1‑151 (MQ=60)
      GGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAC                                                                                                                                                >  SRR14920166.2352846/1‑151 (MQ=60)
      GGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAC                                                                                                                                                >  SRR14920166.1821159/1‑151 (MQ=60)
      GGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAC                                                                                                                                                >  SRR14920166.1821749/1‑151 (MQ=60)
      GGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAAC                                                                                                                                                >  SRR14920166.2078048/1‑151 (MQ=60)
      GGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATGGACAGTATCCGAGGTGATGTCTTCTAAC                                                                                                                                                >  SRR14920166.4628335/1‑151 (MQ=60)
       GTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACC                                                                                                                                               <  SRR14920166.2434373/151‑1 (MQ=60)
        TCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCC                                                                                                                                              <  SRR14920166.298863/151‑1 (MQ=60)
          ATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATCTCTTCTAACCCAC                                                                                                                                            >  SRR14920166.5009921/1‑151 (MQ=60)
           TTTCTTCACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTTACAGTATCCGAGATGATGGCTTCTTACCCACA                                                                                                                                           >  SRR14920166.3541296/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCTGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATCGACGGTATCCGAGATGAGGTCTTCTAACCCCCA                                                                                                                                           >  SRR14920166.3520414/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.2969596/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.2234719/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.3191451/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.3505566/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.3168090/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.4051087/1‑151 (MQ=60)
           TTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAGTGACAGTATCCGAGATGATGTCTTCTAACCCACA                                                                                                                                           >  SRR14920166.4923309/1‑151 (MQ=60)
                TAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTT                                                                                                                                      >  SRR14920166.1085646/1‑151 (MQ=60)
                TAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTT                                                                                                                                      >  SRR14920166.3549513/1‑151 (MQ=60)
                    TCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGA                                                                                                                                  <  SRR14920166.3916297/151‑1 (MQ=60)
                    TCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGA                                                                                                                                  <  SRR14920166.2536950/151‑1 (MQ=60)
                     CATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAA                                                                                                                                 <  SRR14920166.2303076/151‑1 (MQ=60)
                        GCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCTGAGATGATGTCTTCTAACCCACAGGGTTGGGAAAGA                                                                                                                              >  SRR14920166.3501220/1‑151 (MQ=60)
                          GCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGA                                                                                                                            >  SRR14920166.3271854/1‑151 (MQ=60)
                           CATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGCTGTCTTCTAACCCACAGGTTTGGGAAAGCGAC                                                                                                                           >  SRR14920166.2471649/1‑151 (MQ=60)
                           CATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTCACCCACAGGTTTGGGAAAGCGAC                                                                                                                           >  SRR14920166.1795668/1‑151 (MQ=60)
                           CATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGAC                                                                                                                           >  SRR14920166.2471639/1‑151 (MQ=60)
                               GGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAT                                                                                                                       >  SRR14920166.1673514/1‑151 (MQ=60)
                               GGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAT                                                                                                                       >  SRR14920166.1544895/1‑151 (MQ=60)
                               GGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGGTAGAGAAAGCGACAAAT                                                                                                                       >  SRR14920166.3959469/1‑151 (MQ=60)
                               GGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGATTGGGAAAGCGGCAAAT                                                                                                                       >  SRR14920166.2653144/1‑151 (MQ=60)
                                GACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATC                                                                                                                      >  SRR14920166.3798966/1‑151 (MQ=60)
                                GACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATC                                                                                                                      >  SRR14920166.4615837/1‑151 (MQ=60)
                                GACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGGCTTCTAACCCACAGGGTTGGGAAAGCGACAAATC                                                                                                                      >  SRR14920166.3240657/1‑151 (MQ=60)
                                 ACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCT                                                                                                                     <  SRR14920166.2901255/151‑1 (MQ=60)
                                  CAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTC                                                                                                                    >  SRR14920166.3260503/1‑151 (MQ=60)
                                  CAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATCGACAGTATCCGAGATGATGTCTTTTAACCCACAGGTTTGGGAAAGGGACAAATCTC                                                                                                                    >  SRR14920166.4563186/1‑151 (MQ=60)
                                    ATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGAATATGTCTTCTAACCCACAAGGTTGGGAAAGCAACAAATCTCGG                                                                                                                  >  SRR14920166.1286395/1‑151 (MQ=60)
                                     TATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGAGAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTT                                                                                                                 >  SRR14920166.4398090/1‑151 (MQ=60)
                                     TATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTCTGGGAAAGCGACAAATCTCGTT                                                                                                                 >  SRR14920166.2460630/1‑151 (MQ=60)
                                          GCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGG                                                                                                            <  SRR14920166.437339/151‑1 (MQ=60)
                                          GCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGG                                                                                                            <  SRR14920166.4744185/151‑1 (MQ=60)
                                          GCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGAGTGACAGTATCCGAGATGATGTCTTCTAAGCCACAGGTTTGGGAAGGCGACAAAGCTCGTTgtagg                                                                                                            >  SRR14920166.135833/1‑146 (MQ=60)
                                          GCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGAGGGGTGCCGGATGGACAGTATCCGTGATGATGTCTTCTAACCCACAGGTGTGGGAAAGGGACAAAGCTAGTTTACGG                                                                                                            >  SRR14920166.1490884/1‑151 (MQ=60)
                                               GAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGT                                                                                                       >  SRR14920166.3782542/1‑151 (MQ=60)
                                               GAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGT                                                                                                       >  SRR14920166.3782525/1‑151 (MQ=60)
                                                   TCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCAGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCGC                                                                                                   >  SRR14920166.1531351/1‑151 (MQ=60)
                                                     GCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACAT                                                                                                 <  SRR14920166.225509/151‑1 (MQ=60)
                                                      CCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATT                                                                                                <  SRR14920166.171597/151‑1 (MQ=60)
                                                       CTGATCGCAGCGCCCACGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGACATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTG                                                                                               <  SRR14920166.4081509/151‑1 (MQ=60)
                                                        TGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACCGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGA                                                                                              >  SRR14920166.3661701/1‑151 (MQ=60)
                                                           TCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGA                                                                                           <  SRR14920166.1303097/151‑1 (MQ=60)
                                                           TCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGGTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGA                                                                                           >  SRR14920166.2792109/1‑151 (MQ=60)
                                                            CGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGGTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAA                                                                                          >  SRR14920166.1885178/1‑151 (MQ=60)
                                                                  GCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAAACTCGTTTACGGATGGTGCACATTGATGAACCCGGT                                                                                    >  SRR14920166.2515691/1‑151 (MQ=60)
                                                                      ATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTC                                                                                <  SRR14920166.57016/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.462206/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.4202176/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.3785615/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.387616/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.4166373/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.2045518/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.2696343/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.2084509/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.92849/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.2774254/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.2533264/151‑1 (MQ=60)
                                                                          CTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCGCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.1922002/151‑1 (MQ=60)
                                                                          CTGGCATGACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.4068079/151‑1 (MQ=60)
                                                                          CTCGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.4380606/151‑1 (MQ=60)
                                                                          CTCGCATTACAGACAGACCTGTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.4381027/151‑1 (MQ=60)
                                                                          CTAGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAC                                                                            <  SRR14920166.4380607/151‑1 (MQ=60)
                                                                             GCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGAATGGTGCTCATGGATGAACCCGGTATTCGCACCGG                                                                         >  SRR14920166.1537743/1‑151 (MQ=60)
                                                                              CATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCTGATTGACAGTATCCGAGATGATTTCTTTTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTG                                                                        >  SRR14920166.4937174/1‑151 (MQ=60)
                                                                               ATTACAGACAGACTTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCCTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGCTTACGGATGGTGCGCATTGATGAACCCGGTATTTGCACGGTGC                                                                       >  SRR14920166.4849503/1‑151 (MQ=60)
                                                                               ATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCTACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCAGCGTGC                                                                       >  SRR14920166.15043/1‑151 (MQ=60)
                                                                               ATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGC                                                                       >  SRR14920166.3669952/1‑151 (MQ=60)
                                                                               ATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGC                                                                       >  SRR14920166.2381992/1‑151 (MQ=60)
                                                                               ATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGC                                                                       >  SRR14920166.3409037/1‑151 (MQ=60)
                                                                               ATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGC                                                                       >  SRR14920166.2942985/1‑151 (MQ=60)
                                                                                TTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAActgtctcttatacacatctccgagcccacgagacatgcgcagatcgcgtatgccgtctt                                                                      >  SRR14920166.3572427/1‑92 (MQ=60)
                                                                                   CAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAAT                                                                   <  SRR14920166.847183/151‑1 (MQ=60)
                                                                                     GACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTG                                                                 <  SRR14920166.3087385/151‑1 (MQ=60)
                                                                                                     CGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCtgtctcttatacacatctccgagccc                                                 >  SRR14920166.1421232/1‑125 (MQ=60)
                                                                                                      GTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAA                                                >  SRR14920166.2937744/1‑151 (MQ=60)
                                                                                                         GTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGCGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAA                                             >  SRR14920166.3354703/1‑151 (MQ=60)
                                                                                                           GCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAAT                                           >  SRR14920166.4843410/1‑151 (MQ=60)
                                                                                                           GCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAAT                                           <  SRR14920166.3720747/151‑1 (MQ=60)
                                                                                                           GCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTCCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAAT                                           >  SRR14920166.2851937/1‑151 (MQ=60)
                                                                                                              ACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGC                                        >  SRR14920166.4439583/1‑151 (MQ=60)
                                                                                                               CGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCA                                       <  SRR14920166.4770606/151‑1 (MQ=60)
                                                                                                                 AGATGGGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGA                                     >  SRR14920166.2057399/1‑151 (MQ=60)
                                                                                                                      GGAGCCGGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAG                                >  SRR14920166.1471532/1‑151 (MQ=60)
                                                                                                                         GCCGGATTGACAGTATCCGAGATGATGTCTGCTAACCCACAGGTTTGGGAACGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCAC                             <  SRR14920166.2427298/151‑1 (MQ=60)
                                                                                                                            GGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTA                          >  SRR14920166.2711044/1‑151 (MQ=60)
                                                                                                                            GGATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTA                          >  SRR14920166.5087475/1‑151 (MQ=60)
                                                                                                                             GATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTAT                         >  SRR14920166.3962131/1‑151 (MQ=60)
                                                                                                                             GATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTAT                         >  SRR14920166.3881516/1‑151 (MQ=60)
                                                                                                                              ATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATT                        <  SRR14920166.1022257/151‑1 (MQ=60)
                                                                                                                              ATTGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATT                        <  SRR14920166.3184306/151‑1 (MQ=60)
                                                                                                                              ATGGACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATT                        <  SRR14920166.4446672/151‑1 (MQ=60)
                                                                                                                                 GACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAAC                     <  SRR14920166.3527699/151‑1 (MQ=60)
                                                                                                                                 GACAGTATCCGAGATGATGTCTTCTAACCCACAGGTGTGGGAAAGCGACAAATCTCGTGTACGGATGGTGCACAGTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAAC                     >  SRR14920166.3344500/1‑151 (MQ=60)
                                                                                                                                  ACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGACAGAAATGGCAGATGCAGCACGTATTAACG                    >  SRR14920166.2589360/1‑151 (MQ=60)
                                                                                                                                  ACAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACG                    <  SRR14920166.1470125/151‑1 (MQ=60)
                                                                                                                                   CAGTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGT                   <  SRR14920166.3525339/151‑1 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACCGGTTTGGGAAAGCGACAAATCTCGTTTCCGTATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.3833326/1‑151 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.3243197/1‑151 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.2197718/1‑151 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.2387674/1‑151 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.172506/1‑151 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.3043724/1‑151 (MQ=60)
                                                                                                                                     GTATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAACGATCCGAAAGAAATGTCAGATGCAGCACGTATTAACGTGG                 >  SRR14920166.1642228/1‑151 (MQ=60)
                                                                                                                                      TATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCGGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTctgtctct                >  SRR14920166.276835/1‑143 (MQ=60)
                                                                                                                                       ATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAA               >  SRR14920166.2382940/1‑151 (MQ=60)
                                                                                                                                       ATCCGAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAA               >  SRR14920166.1264855/1‑151 (MQ=60)
                                                                                                                                           GAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATTGCAGATGCAGCACGTATTAACGTGGAAAGCG           >  SRR14920166.4134254/1‑151 (MQ=60)
                                                                                                                                           GAGATGATGTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCG           >  SRR14920166.4348988/1‑151 (MQ=60)
                                                                                                                                                   GTCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAG   >  SRR14920166.324762/1‑151 (MQ=60)
                                                                                                                                                    TCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGA  <  SRR14920166.3688759/151‑1 (MQ=60)
                                                                                                                                                    TCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGA  <  SRR14920166.4492848/151‑1 (MQ=60)
                                                                                                                                                    TCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGA  <  SRR14920166.4492585/151‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                      
CAGTATGGTCATTTCTTAACTCATGCGCATCGGACAATATCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACAGACAGACCTTTTCGGACGTTGTGCTACGAGATGGGAGCCGGATTGACAGTATCCGAGATGATGCCTTCTAACCCACAGGTTTGGGAAAGCGACAAATCTCGTTTACGGATGGTGCACATTGATGAACCCGGTATTCGCACCGTGCAAATTGCTGGTAGCGATCCGAAAGAAATGGCAGATGCAGCACGTATTAACGTGGAAAGCGGTGCCCAGA  >  NZ_CP025268/3410943‑3411241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑