Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 258 495.8 21559102 99.2% 21386629 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,957,532 A→G intergenic (‑354/‑318) PA14_RS22700 ← / → PA14_RS22705 16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,957,5320AG99.5% 354.7 / NA 190intergenic (‑354/‑318)PA14_RS22700/PA14_RS2270516S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base G (90/98);  minor base T (1/0);  total (91/99)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

CGTTATTTTCGAAAATTTTCTTTTCTACTCAACCGCTTGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTAT  >  NC_008463/4957418‑4957678
                                                                                                                  |                                                                                                                                                  
                   cttttctACTCAACCGCTTGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCTCCTCGTTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTg                                                                                              >  2:2732323/1‑150 (MQ=255)
                                      gcgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTc                                                                           >  1:1927833/1‑150 (MQ=9)
                                       cgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTcc                                                                          <  2:8098273/150‑1 (MQ=12)
                                       cgcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTcc                                                                          <  2:8098305/150‑1 (MQ=12)
                                       ccccGACCTACAAACCCCCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACTACCTGCCGGAGCGCTTCACCACTcc                                                                          <  1:5570649/148‑1 (MQ=11)
                                        gcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCt                                                                         <  1:1815816/150‑1 (MQ=12)
                                        gcCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCt                                                                         >  2:803374/1‑150 (MQ=12)
                                           gACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTggg                                                                      <  1:4922271/150‑1 (MQ=12)
                                           gACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGATCAAATTCGCTTTCAAGCACCACGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGAGAACAACCTCACGGAGCGCTTCACCAATCATggg                                                                      >  2:4166857/1‑150 (MQ=11)
                                            aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                     >  2:5434498/1‑150 (MQ=12)
                                            aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                     >  2:5321000/1‑150 (MQ=12)
                                             ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                    >  1:4020092/1‑150 (MQ=12)
                                             ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                    <  1:10689185/150‑1 (MQ=12)
                                              cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                   <  1:331084/150‑1 (MQ=12)
                                              cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                   <  1:1084570/150‑1 (MQ=12)
                                                tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagaga                                                                 >  1:4478492/1‑150 (MQ=12)
                                                tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagaga                                                                 <  1:9205829/150‑1 (MQ=12)
                                                 cAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAATTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCTCCACTCCTGGGAGAGaa                                                                >  2:390280/1‑150 (MQ=11)
                                                  aaaCCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAAt                                                               <  2:5783129/150‑1 (MQ=12)
                                                   aaCCATCATCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATc                                                              <  2:7941271/150‑1 (MQ=255)
                                                    aCCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATcc                                                             >  1:3413582/1‑150 (MQ=12)
                                                     ccATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCg                                                            <  2:58585/150‑1 (MQ=12)
                                                     ccATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCg                                                            <  1:8186179/150‑1 (MQ=12)
                                                      cATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGt                                                           <  1:6097351/150‑1 (MQ=12)
                                                      cATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGt                                                           <  1:9845230/150‑1 (MQ=12)
                                                      cATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGt                                                           >  2:2360447/1‑150 (MQ=12)
                                                       aTCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTa                                                          >  1:10142970/1‑150 (MQ=12)
                                                        tCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaa                                                         <  1:3663181/150‑1 (MQ=12)
                                                         cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCTGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                        <  2:548702/150‑1 (MQ=255)
                                                          ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                       <  1:3908547/150‑1 (MQ=12)
                                                          ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                       >  1:6626417/1‑150 (MQ=12)
                                                          ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                       >  1:7657951/1‑150 (MQ=12)
                                                           tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATc                                                      >  1:6792272/1‑150 (MQ=12)
                                                             cGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCt                                                    >  2:6464771/1‑150 (MQ=12)
                                                             cGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGAACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCt                                                    <  2:4724280/150‑1 (MQ=255)
                                                              gATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCtt                                                   >  2:1411476/1‑150 (MQ=12)
                                                              gATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCtt                                                   >  2:6796606/1‑150 (MQ=12)
                                                               aTCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTg                                                  <  1:1194527/150‑1 (MQ=12)
                                                                tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                 <  2:3441908/150‑1 (MQ=12)
                                                                tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                 >  2:3939963/1‑150 (MQ=12)
                                                                 cAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAt                                                <  2:369790/150‑1 (MQ=12)
                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                               <  2:3161532/150‑1 (MQ=12)
                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                               <  2:1081285/150‑1 (MQ=12)
                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                               >  1:7310963/1‑150 (MQ=12)
                                                                  aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                               <  1:10681075/150‑1 (MQ=12)
                                                                   gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                              <  1:8670384/150‑1 (MQ=12)
                                                                   gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                              >  2:4829697/1‑150 (MQ=12)
                                                                   gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                              <  2:1601722/150‑1 (MQ=12)
                                                                    cGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtttt                                             <  1:1504083/150‑1 (MQ=12)
                                                                       gAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCaa                                          >  1:2241083/1‑150 (MQ=12)
                                                                        aGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAg                                         <  1:4257444/150‑1 (MQ=12)
                                                                        aGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAg                                         <  1:5897126/150‑1 (MQ=12)
                                                                         ggCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGACTCAGTAAATCCTTGATTTTCAAGc                                        >  2:9684516/1‑150 (MQ=11)
                                                                         ggCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGc                                        <  2:8185561/150‑1 (MQ=12)
                                                                         ggCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGc                                        >  1:6072159/1‑150 (MQ=12)
                                                                         ggCGCATTCTACAGCGTTCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGc                                        <  2:10556520/150‑1 (MQ=255)
                                                                          gcgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCa                                       <  1:8619328/150‑1 (MQ=12)
                                                                           cgcATTCTACAGCGTTCAACTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTGTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAt                                      <  2:9902683/150‑1 (MQ=11)
                                                                           cgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAt                                      <  1:190758/150‑1 (MQ=12)
                                                                           cgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAt                                      >  1:9460478/1‑150 (MQ=12)
                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                     <  1:6375024/150‑1 (MQ=12)
                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                     <  1:1441583/150‑1 (MQ=12)
                                                                            gcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAtt                                     >  1:5044511/1‑150 (MQ=12)
                                                                              aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                   <  1:5814471/150‑1 (MQ=12)
                                                                               ttCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTAc                                  >  1:3358981/1‑150 (MQ=12)
                                                                                tCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCTAGCATTTAct                                 >  2:6076060/1‑150 (MQ=255)
                                                                                 cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                <  1:2395096/150‑1 (MQ=12)
                                                                                    cAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtc                             <  1:9670187/150‑1 (MQ=12)
                                                                                    cAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtc                             >  1:3124619/1‑150 (MQ=12)
                                                                                    cAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtc                             <  1:8765247/150‑1 (MQ=12)
                                                                                    cAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtc                             <  2:2269661/150‑1 (MQ=12)
                                                                                      gCGTTCACATTCGCTGACAAGCACCTCGGAGACGCTTCGTATCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAGCCTTGATTTTCAAGCATTTACTCTtcgt                           <  2:10259616/150‑1 (MQ=11)
                                                                                       cGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                          <  2:7476397/150‑1 (MQ=12)
                                                                                       cGTTCAAATTCACTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                          <  1:649572/150‑1 (MQ=255)
                                                                                         ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        <  2:4603211/150‑1 (MQ=12)
                                                                                         ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        <  2:1566971/150‑1 (MQ=12)
                                                                                          tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                       <  1:6302660/150‑1 (MQ=12)
                                                                                          tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                       <  1:100997/150‑1 (MQ=12)
                                                                                          tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATATACTCTtcgtcgtc                       >  2:8957239/1‑150 (MQ=255)
                                                                                             aaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGcc                    <  2:5147907/150‑1 (MQ=12)
                                                                                              aTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCg                   >  2:3133598/1‑150 (MQ=12)
                                                                                                tCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCggg                 <  2:4530931/150‑1 (MQ=12)
                                                                                                tCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCggg                 <  1:970357/150‑1 (MQ=12)
                                                                                                tCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTCCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCggg                 >  2:8928595/1‑150 (MQ=255)
                                                                                                 cGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGa                <  2:8513446/150‑1 (MQ=12)
                                                                                                 cGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGa                >  2:10547795/1‑150 (MQ=12)
                                                                                                    tGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAACTCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtg             >  2:2783425/1‑150 (MQ=255)
                                                                                                     gTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtgg            >  1:10187006/1‑150 (MQ=12)
                                                                                                     gTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtgg            <  2:9070853/150‑1 (MQ=12)
                                                                                                       cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtggtg          <  2:5395210/150‑1 (MQ=12)
                                                                                                       cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtggtg          <  2:8150260/150‑1 (MQ=12)
                                                                                                       cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAAACGCTGCGGCGAACAACCTGCAGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAGTTACTCTTCGTCGTCGCCGGGAgtggtg          >  2:3776822/1‑150 (MQ=11)
                                                                                                         aGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcg        >  1:2361751/1‑150 (MQ=12)
                                                                                                          gCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcgc       >  2:1136335/1‑150 (MQ=12)
                                                                                                             ccTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAtt    <  2:5958598/150‑1 (MQ=12)
                                                                                                              cTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATta   <  1:5017745/150‑1 (MQ=12)
                                                                                                              cTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCGGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGGGCGCAGta   >  2:2165354/1‑150 (MQ=11)
                                                                                                               tCGGTGTAGCGTCGTTTCGAATCCCTGTCGGGACCGAACCGCTGCGGCGAACCACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat  <  2:10359740/150‑1 (MQ=11)
                                                                                                               tCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat  >  2:7604685/1‑150 (MQ=12)
                                                                                                               tCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat  >  2:4745145/1‑150 (MQ=12)
                                                                                                                  |                                                                                                                                                  
CGTTATTTTCGAAAATTTTCTTTTCTACTCAACCGCTTGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTAT  >  NC_008463/4957418‑4957678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A