Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 255 480.3 21445252 99.4% 21316580 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,947,645 G→C Q153H (CAG→CAC)  PA14_RS22670 → DNA polymerase Y family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,947,6450GC99.7% 1489.3 / NA 374Q153H (CAG→CAC) PA14_RS22670DNA polymerase Y family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (187/186);  minor base A (1/0);  total (188/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

CCCTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGT‑CAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCG  >  NC_008463/4947521‑4947761
                                                                                                                              |                                                                                                                    
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           >  1:5401619/1‑151 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           >  1:6419759/1‑151 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           <  2:9591548/151‑1 (MQ=255)
    gTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgcg                                                                                        >  2:8735802/1‑151 (MQ=255)
      tCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCt                                                                                      >  1:2717683/1‑151 (MQ=255)
       cGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCACGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGACCCCGGTGGCGGCGCGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTg                                                                                     <  2:4228155/151‑1 (MQ=255)
       cGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTg                                                                                     >  2:491214/1‑151 (MQ=255)
       cGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTg                                                                                     <  1:10463140/151‑1 (MQ=255)
       cGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTg                                                                                     >  2:1032345/1‑151 (MQ=255)
         gCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCa                                                                                   <  2:6248148/151‑1 (MQ=255)
         gCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCa                                                                                   >  1:3451058/1‑151 (MQ=255)
          ccccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCaa                                                                                  >  2:5039267/1‑151 (MQ=255)
            ccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCaccg                                                                                >  2:7785879/1‑148 (MQ=255)
            ccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAacg                                                                                >  1:9488630/1‑150 (MQ=255)
            ccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAacg                                                                                >  2:3056718/1‑150 (MQ=255)
              tGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                              <  1:2497906/151‑1 (MQ=255)
              gggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                              <  2:8642029/150‑1 (MQ=255)
              agcccACGCTACGAGGCCCGCGAGCGCAAGGAACTCACCGCGCTGGGCTTCCGCCACCGCCTGCGCCTGCCCCCGCACCCGGTGGCGGCGAGGGTCCAG‑GCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                              <  1:3875028/148‑1 (MQ=255)
               ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                             >  1:2827187/1‑151 (MQ=255)
                   aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                         <  1:7606634/151‑1 (MQ=255)
                   aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                         >  2:8178864/1‑151 (MQ=255)
                   aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCGGGGCGTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                         <  2:9483855/151‑1 (MQ=255)
                      cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                      <  2:4555930/151‑1 (MQ=255)
                      cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                      >  2:5324552/1‑151 (MQ=255)
                      cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                      <  2:10688758/151‑1 (MQ=255)
                      cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                      >  2:1182330/1‑151 (MQ=255)
                          gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCAGCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                  >  1:7851425/1‑151 (MQ=255)
                           aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                 >  2:6722456/1‑151 (MQ=255)
                           aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                 <  2:9612682/151‑1 (MQ=255)
                            ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                >  2:842484/1‑151 (MQ=255)
                             gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                               >  2:3970015/1‑151 (MQ=255)
                             gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                               <  2:9217918/151‑1 (MQ=255)
                              cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTcc                                                              >  1:9216563/1‑151 (MQ=255)
                              cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTcc                                                              <  2:7811453/151‑1 (MQ=255)
                                cGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGc                                                            <  2:2547223/151‑1 (MQ=255)
                                cGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGc                                                            >  2:1916898/1‑151 (MQ=255)
                                cGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGc                                                            <  1:1393074/151‑1 (MQ=255)
                                 gCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCt                                                           <  1:1620969/151‑1 (MQ=255)
                                  cTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTg                                                          >  2:6311336/1‑151 (MQ=255)
                                   ttGCGCAAGGACCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTgg                                                         <  2:5645586/151‑1 (MQ=255)
                                   ttGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTgg                                                         <  2:10120891/151‑1 (MQ=255)
                                    tGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGa                                                        >  1:5047216/1‑151 (MQ=255)
                                    tGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGa                                                        >  1:8450505/1‑151 (MQ=255)
                                     gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAc                                                       <  2:7165007/151‑1 (MQ=255)
                                     gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAc                                                       >  1:6937319/1‑151 (MQ=255)
                                      cgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                      <  1:10240568/151‑1 (MQ=255)
                                      cgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                      <  2:2837803/151‑1 (MQ=255)
                                      cgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                      <  1:7713817/151‑1 (MQ=255)
                                       gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCg                                                     >  2:6634850/1‑151 (MQ=255)
                                         aaGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGcc                                                   <  1:5985127/151‑1 (MQ=255)
                                         aaGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGcc                                                   <  1:4824215/151‑1 (MQ=255)
                                         aaGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGcc                                                   >  2:3414967/1‑151 (MQ=255)
                                           ggAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                 <  1:6645812/151‑1 (MQ=255)
                                           ggAACTCACGGCGCTGGGCGTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGCGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                 <  2:9677876/151‑1 (MQ=255)
                                             aaCTCACGGCGCTGGGCGTCCGCCATCGCCGGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTggg                                               <  2:4716230/151‑1 (MQ=255)
                                              aCTCTCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                              <  2:1382772/151‑1 (MQ=255)
                                              aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                              >  2:9871716/1‑151 (MQ=255)
                                               cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                             <  2:761926/151‑1 (MQ=255)
                                                tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAct                                            >  1:10101180/1‑151 (MQ=255)
                                                 cACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActc                                           <  1:10559714/151‑1 (MQ=255)
                                                   cGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACtctc                                         <  1:1515323/151‑1 (MQ=255)
                                                   cGGCGCTGGGCTTCCGCCAGCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACtctc                                         <  2:4094800/151‑1 (MQ=255)
                                                    ggCGCTGGGCGTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcg                                        <  2:3653557/151‑1 (MQ=255)
                                                     gcgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgc                                       <  2:7642264/151‑1 (MQ=255)
                                                     gcgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgc                                       >  2:4497164/1‑151 (MQ=255)
                                                        cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCggg                                    <  2:9924971/151‑1 (MQ=255)
                                                        cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCggg                                    >  2:8871394/1‑151 (MQ=255)
                                                         tGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGAACGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                   >  2:5804322/1‑151 (MQ=255)
                                                             cTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTg                               >  1:3970416/1‑151 (MQ=255)
                                                             cTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTg                               <  1:3684388/151‑1 (MQ=255)
                                                              ttCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTgg                              >  1:6869209/1‑151 (MQ=255)
                                                              ttCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTgg                              >  2:7406720/1‑151 (MQ=255)
                                                               tCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                             <  2:3566163/151‑1 (MQ=255)
                                                               tCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                             >  2:8503897/1‑151 (MQ=255)
                                                                ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGCGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                            <  2:3222364/151‑1 (MQ=255)
                                                                ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                            >  2:7293150/1‑151 (MQ=255)
                                                                ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                            <  1:7354595/151‑1 (MQ=255)
                                                                ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                            <  1:9907605/151‑1 (MQ=255)
                                                                ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                            <  2:2011893/151‑1 (MQ=255)
                                                                ccGCCATCGCCTGTGCCAGGCGCCGACCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGTACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGcc                            <  2:7783382/151‑1 (MQ=255)
                                                                 cGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCCCGACGGCCTGGCCAACGAGGACGAGACAGCGCTGCAACGCCAGTTGGCCGCCCTTCAGCGGGACCGCCTGGGACTCTCGCGGGAGCTGGCCg                           >  2:5563519/1‑151 (MQ=255)
                                                                 cGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCg                           <  1:1955218/151‑1 (MQ=255)
                                                                  gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                          <  2:10379342/151‑1 (MQ=255)
                                                                  gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                          >  2:6342331/1‑151 (MQ=255)
                                                                      tCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGcc                      <  1:10336796/151‑1 (MQ=255)
                                                                       cGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGccc                     <  1:4641503/151‑1 (MQ=255)
                                                                        gCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                    <  2:2332461/151‑1 (MQ=255)
                                                                         ccTGGGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCAGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                   <  2:10613047/151‑1 (MQ=255)
                                                                           tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                 >  1:8215438/1‑151 (MQ=255)
                                                                             tgCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc               <  1:1157393/151‑1 (MQ=255)
                                                                              gCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACg              >  1:8939533/1‑151 (MQ=255)
                                                                               ccTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGc             <  1:5774471/151‑1 (MQ=255)
                                                                                cTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCa            <  2:5442342/151‑1 (MQ=255)
                                                                                cTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCa            >  1:8620610/1‑151 (MQ=255)
                                                                                 tGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCAt           >  2:3015374/1‑151 (MQ=255)
                                                                                    cgcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATggg        >  2:6912894/1‑151 (MQ=255)
                                                                                        gAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTg    >  1:5135009/1‑151 (MQ=255)
                                                                                         aaCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc   <  1:9359273/151‑1 (MQ=255)
                                                                                         aaCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc   >  2:5490681/1‑151 (MQ=255)
                                                                                          aCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgcg  <  1:430470/151‑1 (MQ=255)
                                                                                                                              |                                                                                                                    
CCCTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGT‑CAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCG  >  NC_008463/4947521‑4947761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  NC_008463/4947546‑4947739
                                                                                                    |                                                                                              
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                             >  SRR18135083.6604606/1‑151 (MQ=60)
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                             >  SRR18135083.3766063/1‑151 (MQ=60)
 GAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCC                                            >  SRR18135083.9309035/1‑151 (MQ=60)
  CGGCCCGCTTGCGCAAGGAACTCCCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCT                                           <  SRR18135083.4671121/151‑1 (MQ=60)
   GGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGNCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTT                                          >  SRR18135083.5659247/1‑151 (MQ=60)
    GCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTC                                         >  SRR18135083.8591983/1‑151 (MQ=60)
     CCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCC                                        >  SRR18135083.9253425/1‑151 (MQ=60)
     CCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCC                                        <  SRR18135083.4872510/151‑1 (MQ=60)
      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                       <  SRR18135083.4584663/151‑1 (MQ=60)
      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                       <  SRR18135083.4589818/151‑1 (MQ=60)
       CGCTTGCGCAAGGACCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑ACCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGC                                      <  SRR18135083.397699/151‑1 (MQ=60)
       CGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGC                                      >  SRR18135083.10019013/1‑151 (MQ=60)
       CGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGC                                      <  SRR18135083.1399278/151‑1 (MQ=60)
        GCTTGCGCAAGTAACTCACGGCGCTTGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTACCGCT                                     >  SRR18135083.5385416/1‑151 (MQ=56)
        GCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCT                                     <  SRR18135083.1627996/151‑1 (MQ=60)
        GCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTGCCGCT                                     >  SRR18135083.5385424/1‑151 (MQ=60)
         CTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTG                                    >  SRR18135083.10200304/1‑151 (MQ=60)
          TTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGG                                   >  SRR18135083.7653593/1‑151 (MQ=60)
           TGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGA                                  >  SRR18135083.8484415/1‑151 (MQ=60)
           TGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGA                                  >  SRR18135083.5068648/1‑151 (MQ=60)
           TGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGA                                  <  SRR18135083.9568860/151‑1 (MQ=60)
            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                 >  SRR18135083.6965768/1‑151 (MQ=60)
            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                 >  SRR18135083.6965795/1‑151 (MQ=60)
             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135083.10281132/151‑1 (MQ=60)
             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135083.6534475/151‑1 (MQ=60)
             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135083.8747240/151‑1 (MQ=60)
             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135083.1550166/151‑1 (MQ=60)
             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135083.7745068/151‑1 (MQ=60)
              GCAAGGAACTCACGGTGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCG                               >  SRR18135083.5153542/1‑151 (MQ=60)
              GCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCG                               <  SRR18135083.7930512/151‑1 (MQ=60)
                AAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCC                             >  SRR18135083.9103678/1‑151 (MQ=60)
                AAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCC                             <  SRR18135083.4844698/151‑1 (MQ=60)
                AAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCC                             <  SRR18135083.6010167/151‑1 (MQ=60)
                 AGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCT                            >  SRR18135083.9111755/1‑151 (MQ=60)
                 AGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCT                            <  SRR18135083.5859751/151‑1 (MQ=60)
                  GGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTG                           <  SRR18135083.7155754/151‑1 (MQ=60)
                  GGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTG                           <  SRR18135083.6673199/151‑1 (MQ=60)
                  GGAACTCACGGCGCTGGGCTTCAGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTG                           <  SRR18135083.3064044/151‑1 (MQ=60)
                    AACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGG                         >  SRR18135083.5142398/1‑151 (MQ=60)
                     ACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGA                        >  SRR18135083.6523159/1‑151 (MQ=60)
                      CTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAC                       >  SRR18135083.6040382/1‑151 (MQ=60)
                       TCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACT                      <  SRR18135083.7081229/151‑1 (MQ=60)
                       TCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACT                      >  SRR18135083.10141247/1‑151 (MQ=60)
                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCGGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     >  SRR18135083.1058463/1‑151 (MQ=60)
                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     <  SRR18135083.1495098/151‑1 (MQ=60)
                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     <  SRR18135083.10601356/151‑1 (MQ=60)
                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                    <  SRR18135083.8498532/151‑1 (MQ=60)
                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                    >  SRR18135083.2701829/1‑151 (MQ=60)
                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                    >  SRR18135083.3378118/1‑151 (MQ=60)
                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   <  SRR18135083.1515196/151‑1 (MQ=60)
                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   <  SRR18135083.1521966/151‑1 (MQ=60)
                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   >  SRR18135083.6957806/1‑151 (MQ=60)
                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   <  SRR18135083.4657745/151‑1 (MQ=60)
                           GGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCG                  <  SRR18135083.7785688/151‑1 (MQ=60)
                           GGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCG                  <  SRR18135083.10387593/151‑1 (MQ=60)
                            GCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGC                 <  SRR18135083.1079433/151‑1 (MQ=60)
                             CGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCG                <  SRR18135083.3725219/151‑1 (MQ=60)
                              GCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGG               <  SRR18135083.10368699/151‑1 (MQ=60)
                                TGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA             >  SRR18135083.10376721/1‑151 (MQ=60)
                                TGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA             <  SRR18135083.2786839/151‑1 (MQ=60)
                                TGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA             >  SRR18135083.2341989/1‑151 (MQ=60)
                                TGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA             >  SRR18135083.10377361/1‑151 (MQ=60)
                                  GGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGC           >  SRR18135083.3216/1‑151 (MQ=60)
                                  GGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGC           <  SRR18135083.5884822/151‑1 (MQ=60)
                                   GCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCT          <  SRR18135083.10761474/151‑1 (MQ=60)
                                    CTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTG         <  SRR18135083.3699950/151‑1 (MQ=60)
                                    CTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTG         >  SRR18135083.3987217/1‑151 (MQ=60)
                                     TTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGG        >  SRR18135083.6897427/1‑151 (MQ=60)
                                     TTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGG        >  SRR18135083.6274781/1‑151 (MQ=60)
                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      <  SRR18135083.9946909/151‑1 (MQ=60)
                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      >  SRR18135083.1861178/1‑151 (MQ=60)
                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      <  SRR18135083.7384612/151‑1 (MQ=60)
                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      <  SRR18135083.6077263/151‑1 (MQ=60)
                                        CGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCG     <  SRR18135083.8662991/151‑1 (MQ=60)
                                        CGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCG     <  SRR18135083.1963720/151‑1 (MQ=60)
                                         GCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGT    >  SRR18135083.7970838/1‑151 (MQ=60)
                                          CCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTC   <  SRR18135083.4003741/151‑1 (MQ=60)
                                          CCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCAAGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCATGGGACTCTCGCGGGAGCTGGCCGTC   >  SRR18135083.5108119/1‑151 (MQ=60)
                                           CATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  <  SRR18135083.4734120/151‑1 (MQ=60)
                                                                                                    |                                                                                              
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  NC_008463/4947546‑4947739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑