Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 255 480.3 21445252 99.4% 21316580 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,205,906 Δ1 bp intergenic (‑11/+186) PA14_RS28345 ← / ← PA14_RS28350 type VI secretion system tip protein VgrG/Hcp family type VI secretion system effector

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,205,9030C.100.0% 427.4 / NA 265intergenic (‑8/+189)PA14_RS28345/PA14_RS28350type VI secretion system tip protein VgrG/Hcp family type VI secretion system effector
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (129/136);  total (129/136)

Only 100 of 286 total aligned reads displayed.

ACGGGGCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTCCCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTT  >  NC_008463/6205753‑6206019
                                                                                                                                                      |                                                                                                                    
aCGGGGCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTc                                                                                                                      <  2:460274/151‑1 (MQ=31)
  ggggCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTccc                                                                                                                    >  1:9796689/1‑151 (MQ=31)
  ggggCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTccc                                                                                                                    <  1:3363732/151‑1 (MQ=31)
   gggCGTTCGCCGTCCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTccct                                                                                                                   <  2:9035602/151‑2 (MQ=14)
   gggCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTccct                                                                                                                   <  2:10118632/151‑2 (MQ=14)
   gggCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCGGGTTGGCTGCTGCGAACATCCGTTGTccct                                                                                                                   >  1:2645978/1‑150 (MQ=14)
     gCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTccctca                                                                                                                 <  2:1544986/151‑4 (MQ=1)
                         ggTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattc                                                                                            <  2:3456964/151‑1 (MQ=11)
                            cGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattcgat                                                                                         >  1:8271182/1‑151 (MQ=11)
                            cGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattcgat                                                                                         <  2:6561968/151‑1 (MQ=11)
                             gAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattcgatt                                                                                        >  1:10483991/1‑151 (MQ=11)
                             gAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattcgatt                                                                                        <  1:50123/151‑1 (MQ=11)
                             gAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattcgatt                                                                                        >  1:9155594/1‑151 (MQ=11)
                             gAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACcgattcgatt                                                                                        <  1:7198125/151‑1 (MQ=11)
                              aaGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTg                                                                                       <  1:9621085/151‑1 (MQ=11)
                                ggCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTc                                                                                     <  1:8552654/151‑1 (MQ=11)
                                 gCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcc                                                                                    >  1:8895913/1‑151 (MQ=11)
                                  cGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcct                                                                                   >  2:7128080/1‑151 (MQ=11)
                                  cGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcct                                                                                   <  2:8654280/151‑1 (MQ=11)
                                  cGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcct                                                                                   <  2:8945326/151‑1 (MQ=11)
                                  cGAACGGCTGGCTGATGGCTTCGCGGCCGCGGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcct                                                                                   >  2:7550603/1‑151 (MQ=2)
                                   gAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcctg                                                                                  >  2:7224473/1‑151 (MQ=11)
                                   gAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcctg                                                                                  >  2:5054539/1‑151 (MQ=11)
                                   gAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcctg                                                                                  >  1:4547135/1‑151 (MQ=11)
                                       ggctggctGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcctgcctg                                                                              >  2:4276640/1‑151 (MQ=11)
                                       ggctggctGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTcctgcctg                                                                              <  1:1329392/151‑1 (MQ=11)
                                        gctggctgATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTgg                                                                             <  1:2540729/151‑1 (MQ=11)
                                           ggctgATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGgcgc                                                                          >  1:3914391/1‑151 (MQ=11)
                                             ctgATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCgg                                                                        <  1:1720367/151‑1 (MQ=11)
                                              tgATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCggg                                                                       >  2:1003618/1‑151 (MQ=11)
                                               gATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGc                                                                      <  2:5310270/151‑1 (MQ=11)
                                               gATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGc                                                                      <  1:8153458/151‑1 (MQ=11)
                                                agggCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCg                                                                     <  2:8743704/149‑1 (MQ=11)
                                                   gCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCgaga                                                                  <  2:2538393/151‑1 (MQ=11)
                                                     ttCGCGGCCGCTGCAGGAGAGCACCTGCAGGGCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACa                                                                <  2:9758699/151‑1 (MQ=2)
                                                     ttCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACa                                                                <  2:6971221/151‑1 (MQ=11)
                                                     ttCGCGGCCGCGGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAACGGGTCTGGTTGGCTGCTGCGACCATCCGTTGT‑CCCTCACTGGCGCAGGCGCCCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACa                                                                <  2:5469891/151‑1 (MQ=11)
                                                       cgcgGCCGCTGACGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAg                                                              <  1:9060272/151‑1 (MQ=2)
                                                       cgcgGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGATGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAg                                                              <  1:7074338/151‑1 (MQ=2)
                                                        gcgGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAgg                                                             <  1:7575235/151‑1 (MQ=11)
                                                        gcgGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAgg                                                             <  2:273626/151‑1 (MQ=11)
                                                          ggCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgc                                                           >  1:5501493/1‑151 (MQ=11)
                                                          ggCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgc                                                           >  2:8151030/1‑151 (MQ=11)
                                                          ggCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgc                                                           >  1:634865/1‑151 (MQ=11)
                                                          ggCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACGGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgc                                                           >  1:4707365/1‑151 (MQ=2)
                                                           gCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGTGGCTGAAATGGGTGTGGTTGGCTGCTGCGAACAGCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgcg                                                          >  2:4179028/1‑151 (MQ=2)
                                                           gCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgcg                                                          >  1:191069/1‑151 (MQ=11)
                                                            ccGCTGAAGGAGAGGACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgcgc                                                         <  2:9132186/151‑1 (MQ=2)
                                                             cGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgcgcg                                                        >  2:5870352/1‑151 (MQ=11)
                                                              gCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGgcgcgc                                                       <  1:7882931/151‑1 (MQ=11)
                                                               cTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCa                                                      >  2:3839863/1‑151 (MQ=11)
                                                                 gAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAgg                                                    <  2:896320/151‑1 (MQ=11)
                                                                  aaGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGc                                                   >  2:8355857/1‑151 (MQ=11)
                                                                  aaGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGc                                                   <  2:9390279/151‑1 (MQ=11)
                                                                       gagCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGAt                                              >  2:1688093/1‑151 (MQ=11)
                                                                        agCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATc                                             <  1:94143/151‑1 (MQ=11)
                                                                        agCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATc                                             <  1:8801644/151‑1 (MQ=11)
                                                                        agCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATc                                             <  2:1133480/151‑1 (MQ=11)
                                                                           aCCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGa                                          <  1:4181172/151‑1 (MQ=11)
                                                                           aCCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGa                                          >  1:7392559/1‑151 (MQ=11)
                                                                            ccTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGaa                                         <  2:7858907/151‑1 (MQ=11)
                                                                             cTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAg                                        <  2:8714639/151‑1 (MQ=11)
                                                                              tGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGc                                       >  1:3077052/1‑151 (MQ=11)
                                                                               gCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCt                                      <  1:3430811/151‑1 (MQ=11)
                                                                               gCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCt                                      <  1:3188980/151‑1 (MQ=11)
                                                                                cAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTg                                     >  2:4828251/1‑151 (MQ=11)
                                                                                 aGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGc                                    >  2:10616930/1‑151 (MQ=11)
                                                                                 aGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGc                                    >  1:2338813/1‑151 (MQ=11)
                                                                                     cGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTac                                <  1:8228526/151‑1 (MQ=11)
                                                                                     cGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTac                                >  1:6102834/1‑151 (MQ=11)
                                                                                      gAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTaca                               <  1:6278212/151‑1 (MQ=11)
                                                                                      gAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTaca                               <  2:772039/151‑1 (MQ=11)
                                                                                      gAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTaca                               >  2:6722129/1‑151 (MQ=11)
                                                                                        gggCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTacaac                             <  2:5753966/151‑1 (MQ=11)
                                                                                        gggCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGGTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTacaac                             >  2:7924792/1‑151 (MQ=2)
                                                                                         ggCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTacaaca                            >  1:1505083/1‑151 (MQ=11)
                                                                                          gCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTAcaacaa                           <  2:3223505/151‑1 (MQ=11)
                                                                                            gAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAg                         >  2:2894680/1‑151 (MQ=11)
                                                                                            gAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAg                         >  1:2209839/1‑151 (MQ=11)
                                                                                              ggCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCg                       <  2:204437/151‑1 (MQ=11)
                                                                                                ccGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGc                     >  2:3682479/1‑151 (MQ=11)
                                                                                                  gTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCAt                   >  1:9906747/1‑151 (MQ=11)
                                                                                                  gTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCAt                   >  1:5917605/1‑151 (MQ=11)
                                                                                                      aTGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGAc               <  1:7546043/151‑1 (MQ=11)
                                                                                                       tGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGAcc              >  2:6425363/1‑151 (MQ=11)
                                                                                                       tGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGAcc              <  1:4894038/151‑1 (MQ=11)
                                                                                                        ggCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGAccc             <  1:3965366/151‑1 (MQ=11)
                                                                                                         gCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCg            <  1:1014736/151‑1 (MQ=11)
                                                                                                           gAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGgc          <  1:5147183/151‑1 (MQ=11)
                                                                                                            aGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGgcg         >  2:6194725/1‑151 (MQ=11)
                                                                                                            aGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGgcg         >  1:2573887/1‑151 (MQ=11)
                                                                                                             ggCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGgcgc        >  2:9259810/1‑151 (MQ=11)
                                                                                                             ggCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGgcgc        <  1:1844890/151‑1 (MQ=11)
                                                                                                             ggCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGgcgc        <  1:6654164/151‑1 (MQ=11)
                                                                                                               cTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCgg      <  2:6863218/151‑1 (MQ=11)
                                                                                                               cTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCgg      <  1:2845910/151‑1 (MQ=11)
                                                                                                               cTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCgg      <  1:4411954/151‑1 (MQ=11)
                                                                                                                tGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCggg     >  1:9291269/1‑151 (MQ=11)
                                                                                                                 gAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGc    <  2:9522603/151‑1 (MQ=11)
                                                                                                                   aaTGGGTCTGGTTGGCTGCTGCGAACATCCGTTGT‑CCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCtt  >  2:1898034/1‑151 (MQ=1)
                                                                                                                                                      |                                                                                                                    
ACGGGGCGTTCGCCGACCAGCTCCAGGTCGAAGGCGAACGGCTGGCTGATGGCTTCGCGGCCGCTGAAGGAGAGCACCTGCAGGTCGAGGGCGAGGCCGTCGATGGCGAGGCTGAAATGGGTCTGGTTGGCTGCTGCGAACATCCGTTGTCCCCTCACTGGCGCAGGCGACCGATTCGATTGTCCTGCCTGGCGCGGGCGAGACAAGGGCGCGCAGGCTCGATCGGAAGCTGCCTACAACAAAGCGGCATCGACCCGGCGCGGGCTT  >  NC_008463/6205753‑6206019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A