Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 259 502.6 21734322 99.3% 21582181 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,545,663 T→C I527I (ATT→ATC)  tssI → type VI secretion system tip protein TssI/VgrG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,545,6630TC99.5% 777.1 / NA 208I527I (ATT→ATC) tssItype VI secretion system tip protein TssI/VgrG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (86/121);  minor base A (0/1);  total (86/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTC  >  NC_008463/2545588‑2545810
                                                                           |                                                                                                                                                   
 gCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATg                                                                         <  2:4688401/151‑1 (MQ=255)
  ccGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGc                                                                        <  2:8555993/151‑1 (MQ=255)
   cGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGcc                                                                       <  2:4885905/151‑1 (MQ=255)
     agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa                                                                     >  2:7177879/1‑151 (MQ=255)
     agagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCaa                                                                     <  1:3047338/151‑1 (MQ=255)
      gagGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAc                                                                    >  2:3294632/1‑151 (MQ=255)
       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                                                   >  1:5784136/1‑151 (MQ=255)
       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                                                   >  1:5785150/1‑151 (MQ=255)
       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                                                   >  2:2003603/1‑151 (MQ=255)
       agGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAAcc                                                                   <  1:6665097/151‑1 (MQ=255)
        ggAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCg                                                                  >  2:9482832/1‑151 (MQ=255)
         gAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgc                                                                 <  2:7796801/151‑1 (MQ=255)
           aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcag                                                               >  2:2739714/1‑151 (MQ=255)
            ccAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagc                                                              >  1:3291789/1‑151 (MQ=255)
             cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca                                                             >  2:4617425/1‑151 (MQ=255)
             cAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCgcagca                                                             <  1:9406403/151‑1 (MQ=255)
              aGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCaa                                                            >  1:1233948/1‑151 (MQ=255)
               ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAg                                                           <  1:10403667/151‑1 (MQ=255)
                 aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc                                                         <  2:3105245/151‑1 (MQ=255)
                 aCATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGAGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGAc                                                         >  2:241621/1‑151 (MQ=14)
                  cATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACg                                                        >  1:4928350/1‑151 (MQ=255)
                   atcgAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACgg                                                       >  1:457724/4‑151 (MQ=255)
                   atcgAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACgg                                                       <  1:8712736/148‑1 (MQ=255)
                    tcgAGGTCGAGAACGACGAGAGCCCCTGGGTCGGCCACGACCGGACCAAGAACATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACCGCACCGATACCATCACCGTGCATGCCTACAGCAGCAAGACGGt                                                      >  2:5226434/3‑151 (MQ=14)
                     cgAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTc                                                     <  1:5059348/150‑1 (MQ=255)
                      gAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCg                                                    >  2:5746709/1‑151 (MQ=255)
                      gAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCg                                                    <  2:6030843/151‑1 (MQ=255)
                      gAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCg                                                    >  1:7138705/1‑151 (MQ=255)
                       aGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGa                                                   >  1:10517755/1‑151 (MQ=255)
                        ggTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCTCCGTGCATGCCAACCGCAGCAAGACGGTCGAc                                                  <  2:4427306/151‑1 (MQ=255)
                        ggTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAc                                                  <  2:308495/151‑1 (MQ=255)
                        ggTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAc                                                  <  1:8030780/151‑1 (MQ=255)
                        ggTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAc                                                  <  1:7555934/151‑1 (MQ=255)
                         gtcgagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAcc                                                 <  2:8986939/148‑1 (MQ=255)
                         gtcgagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCAGGCCAACCGCAGCAAGACGGTCGAcc                                                 >  2:8527772/4‑151 (MQ=255)
                          tcgagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCg                                                <  2:3465019/149‑1 (MQ=255)
                            gagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa                                              >  2:9219430/1‑151 (MQ=255)
                            gagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa                                              >  2:3236295/1‑151 (MQ=255)
                            gagaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCa                                              <  2:9841784/151‑1 (MQ=255)
                             agaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCaa                                             <  2:5423885/151‑1 (MQ=255)
                              gaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAAc                                            <  1:9580242/151‑1 (MQ=255)
                               aaCGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACg                                           <  2:403093/151‑1 (MQ=255)
                                acgacgAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACga                                          >  2:9131427/1‑151 (MQ=255)
                                acgacgAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACga                                          >  1:4374805/1‑151 (MQ=255)
                                 cgacgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgag                                         >  1:10190242/1‑151 (MQ=255)
                                 cgacgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgag                                         >  2:8249780/1‑151 (MQ=255)
                                  gacgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACgaga                                        <  2:1269790/151‑1 (MQ=255)
                                    cgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACg                                      <  2:3796532/151‑1 (MQ=255)
                                    cgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACg                                      <  2:5730369/151‑1 (MQ=255)
                                     gagaGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACgg                                     >  2:1529204/1‑151 (MQ=255)
                                       gagCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTc                                   <  1:9877784/151‑1 (MQ=255)
                                       gagCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTc                                   <  2:10532376/151‑1 (MQ=255)
                                       gagCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTc                                   >  2:5438953/1‑151 (MQ=255)
                                        agCCACTGGGTCGGCCACGACCGGACCAAGACCACCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCa                                  <  1:8478511/151‑1 (MQ=255)
                                          ccactgggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAgg                                <  1:257932/146‑1 (MQ=255)
                                           cactgggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGa                               >  1:5900660/5‑151 (MQ=255)
                                           cactgggTCGGCCACGACCGGACCAAGACAATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGa                               >  2:118294/5‑151 (MQ=255)
                                             ctgggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATc                             <  2:5125294/149‑1 (MQ=255)
                                             ctgggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATc                             <  1:2017881/149‑1 (MQ=255)
                                              tgggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCg                            <  2:1789328/150‑1 (MQ=255)
                                               gggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCgg                           <  1:698076/151‑1 (MQ=255)
                                               gggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCgg                           <  1:4392072/151‑1 (MQ=255)
                                               gggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCgg                           <  2:10170624/151‑1 (MQ=255)
                                                ggTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGc                          <  2:967419/151‑1 (MQ=255)
                                                 gTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCa                         <  1:10598380/151‑1 (MQ=255)
                                                  tCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAt                        <  2:8642326/151‑1 (MQ=255)
                                                  tCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAt                        <  1:2974891/151‑1 (MQ=255)
                                                  tCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAt                        >  1:2414779/1‑151 (MQ=255)
                                                   cGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATg                       <  2:7646813/151‑1 (MQ=255)
                                                       cACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACa                   <  1:3234713/151‑1 (MQ=255)
                                                       cACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACa                   <  2:4276288/151‑1 (MQ=255)
                                                         cGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAg                 >  1:10329156/1‑151 (MQ=255)
                                                          gACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACaata                >  1:3671047/1‑149 (MQ=255)
                                                          gACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGa                >  2:6322169/1‑151 (MQ=255)
                                                          gACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGa                >  1:7453969/1‑151 (MQ=255)
                                                          gACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGa                >  1:2636400/1‑151 (MQ=255)
                                                           aCCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTTGACAACAACGAGACCATCACCGTGAATGACAACCGCAGCAAGACGGTCGACCGCACCGAGACGGTCTGGATCGGCATGAACACGAc               >  2:1883966/1‑151 (MQ=21)
                                                             cGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACgg             <  1:9697063/151‑1 (MQ=255)
                                                             cGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACgg             >  1:5284439/1‑151 (MQ=255)
                                                               gACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGaa           <  1:38909/151‑1 (MQ=255)
                                                               gACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGaa           <  2:5582420/151‑1 (MQ=255)
                                                               gACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGaa           <  1:9758426/151‑1 (MQ=255)
                                                                aCCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGaaa          <  2:5321885/151‑1 (MQ=255)
                                                                aCCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGaaa          <  1:2802060/151‑1 (MQ=255)
                                                                aCCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGaaa          >  1:2227597/1‑151 (MQ=255)
                                                                  cAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAAcc        >  1:2184066/1‑151 (MQ=255)
                                                                  cAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAAcc        >  2:6422572/1‑151 (MQ=255)
                                                                  cAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAAcc        >  1:9598246/1‑151 (MQ=255)
                                                                  cAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAAcc        <  2:10797944/151‑1 (MQ=255)
                                                                   aaGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCa       >  1:9044866/1‑151 (MQ=255)
                                                                   aaGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCa       >  2:298690/1‑151 (MQ=255)
                                                                   aaGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCa       >  1:6173761/1‑151 (MQ=255)
                                                                     gACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATc     <  2:7219986/151‑1 (MQ=255)
                                                                     gACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATc     <  2:10592017/151‑1 (MQ=255)
                                                                      aCCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATcc    >  1:1791012/1‑151 (MQ=255)
                                                                      aCCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATcc    >  1:7875080/1‑151 (MQ=255)
                                                                      aCCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATcc    >  2:655980/1‑151 (MQ=255)
                                                                       ccATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCt   <  2:3641847/151‑1 (MQ=255)
                                                                        cATCGACCACGACGAGACGGTGCATGTGAAGCACTATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTc  <  2:3431846/151‑1 (MQ=255)
                                                                        cATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTc  <  1:4636824/151‑1 (MQ=255)
                                                                           |                                                                                                                                                   
TGCCGAGAGGAACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTC  >  NC_008463/2545588‑2545810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTCAT  >  NC_008463/2545599‑2545812
                                                                |                                                                                                                                                     
ACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAG                                                                 <  SRR18135085.3295822/151‑1 (MQ=0)
 CCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGC                                                                >  SRR18135085.3304583/1‑151 (MQ=1)
 CCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGC                                                                <  SRR18135085.10631483/151‑1 (MQ=1)
  CAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCA                                                               <  SRR18135085.9439884/151‑1 (MQ=3)
  CAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCA                                                               <  SRR18135085.5005200/151‑1 (MQ=3)
   AGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAA                                                              >  SRR18135085.1238894/1‑151 (MQ=5)
    GGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAG                                                             <  SRR18135085.10440499/151‑1 (MQ=6)
    GGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAG                                                             <  SRR18135085.10440199/151‑1 (MQ=6)
       CATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACG                                                          >  SRR18135085.4947824/1‑151 (MQ=17)
       CATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACG                                                          >  SRR18135085.4947606/1‑151 (MQ=17)
        ATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGG                                                         <  SRR18135085.8744041/151‑1 (MQ=21)
        ATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGG                                                         >  SRR18135085.459690/1‑151 (MQ=21)
         TCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGT                                                        <  SRR18135085.262172/151‑1 (MQ=25)
         TCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGT                                                        <  SRR18135085.1809751/151‑1 (MQ=25)
          CGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTC                                                       <  SRR18135085.5079123/151‑1 (MQ=29)
          CGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTC                                                       <  SRR18135085.79908/151‑1 (MQ=29)
           GAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCG                                                      <  SRR18135085.3504996/151‑1 (MQ=33)
           GAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCG                                                      >  SRR18135085.7165090/1‑151 (MQ=33)
           GAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCG                                                      >  SRR18135085.7165091/1‑151 (MQ=33)
            AGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGA                                                     <  SRR18135085.4194824/151‑1 (MQ=35)
            AGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGA                                                     >  SRR18135085.10554669/1‑151 (MQ=35)
             GGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAC                                                    <  SRR18135085.7583657/151‑1 (MQ=39)
             GGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGAC                                                    <  SRR18135085.8059879/151‑1 (MQ=39)
              GTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACC                                                   <  SRR18135085.9810740/151‑1 (MQ=43)
                CGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGC                                                 >  SRR18135085.8342163/1‑151 (MQ=51)
                 GAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCA                                                >  SRR18135085.7839690/1‑151 (MQ=56)
                   GAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAAC                                              <  SRR18135085.9614242/151‑1 (MQ=60)
                    AACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACG                                             >  SRR18135085.9657114/1‑151 (MQ=60)
                    AACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACG                                             <  SRR18135085.5877822/151‑1 (MQ=60)
                     ACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGA                                            >  SRR18135085.4391813/1‑151 (MQ=60)
                      CGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAG                                           <  SRR18135085.4597409/151‑1 (MQ=60)
                      CGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAG                                           >  SRR18135085.10226092/1‑151 (MQ=60)
                        ACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGAC                                         <  SRR18135085.1166156/151‑1 (MQ=60)
                          GAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGG                                       >  SRR18135085.6149539/1‑151 (MQ=60)
                           AGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGT                                      <  SRR18135085.7414184/151‑1 (MQ=60)
                            GAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTC                                     <  SRR18135085.9912611/151‑1 (MQ=60)
                             AGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCA                                    >  SRR18135085.4502112/1‑151 (MQ=60)
                             AGCCACTGGGTCGGCCACGACCGGACCAAGACCACCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCA                                    <  SRR18135085.8509093/151‑1 (MQ=60)
                              GCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAG                                   <  SRR18135085.8807625/151‑1 (MQ=60)
                               ccactGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGG                                  <  SRR18135085.246377/146‑1 (MQ=60)
                               ccactGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGG                                  <  SRR18135085.259098/146‑1 (MQ=60)
                                cactGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGA                                 >  SRR18135085.5923410/5‑151 (MQ=60)
                                cactGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGA                                 >  SRR18135085.8476322/5‑151 (MQ=60)
                                  ctGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATC                               >  SRR18135085.8621478/3‑151 (MQ=60)
                                  ctGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATC                               <  SRR18135085.6349624/149‑1 (MQ=60)
                                  ctGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATC                               <  SRR18135085.2025707/149‑1 (MQ=60)
                                   TGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCG                              <  SRR18135085.2151131/151‑1 (MQ=60)
                                    GGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGG                             <  SRR18135085.4409114/151‑1 (MQ=60)
                                    GGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGG                             <  SRR18135085.700921/151‑1 (MQ=60)
                                    GGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGG                             <  SRR18135085.4409133/151‑1 (MQ=60)
                                     GGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGC                            <  SRR18135085.7528426/151‑1 (MQ=60)
                                      GTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCA                           <  SRR18135085.10635532/151‑1 (MQ=60)
                                       TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAT                          >  SRR18135085.2424145/1‑151 (MQ=60)
                                       TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAT                          <  SRR18135085.5644039/151‑1 (MQ=60)
                                       TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAT                          <  SRR18135085.2986536/151‑1 (MQ=60)
                                       TCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCAT                          <  SRR18135085.2986550/151‑1 (MQ=60)
                                        CGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATG                         <  SRR18135085.6801097/151‑1 (MQ=60)
                                        CGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATG                         >  SRR18135085.9469957/1‑151 (MQ=60)
                                          GCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAA                       <  SRR18135085.2913467/151‑1 (MQ=60)
                                           CCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAAC                      >  SRR18135085.1388394/1‑151 (MQ=60)
                                            CACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACA                     <  SRR18135085.3247306/151‑1 (MQ=60)
                                            CACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACA                     >  SRR18135085.9235295/1‑151 (MQ=60)
                                              CGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAG                   >  SRR18135085.10365436/1‑151 (MQ=60)
                                              CGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAG                   >  SRR18135085.10365464/1‑151 (MQ=60)
                                              CGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAG                   <  SRR18135085.5297437/151‑1 (MQ=60)
                                               GACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAATA                  >  SRR18135085.3685322/1‑151 (MQ=60)
                                               GACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGA                  >  SRR18135085.7481347/1‑151 (MQ=60)
                                               GACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGA                  >  SRR18135085.2646614/1‑151 (MQ=60)
                                               GACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGA                  >  SRR18135085.3792840/1‑151 (MQ=60)
                                                ACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGAC                 <  SRR18135085.4817645/151‑1 (MQ=60)
                                                  CGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGG               >  SRR18135085.5305081/1‑151 (MQ=60)
                                                  CGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGG               <  SRR18135085.9731405/151‑1 (MQ=60)
                                                  CGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGG               <  SRR18135085.9731401/151‑1 (MQ=60)
                                                    GACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAA             <  SRR18135085.39123/151‑1 (MQ=60)
                                                    GACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAA             <  SRR18135085.9792907/151‑1 (MQ=60)
                                                     ACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAA            <  SRR18135085.2812945/151‑1 (MQ=60)
                                                     ACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAA            >  SRR18135085.2236283/1‑151 (MQ=60)
                                                      CCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAAC           <  SRR18135085.22442/151‑1 (MQ=60)
                                                       CAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACC          >  SRR18135085.2192586/1‑151 (MQ=60)
                                                       CAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACC          >  SRR18135085.9632291/1‑151 (MQ=60)
                                                        AAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCA         >  SRR18135085.279318/1‑151 (MQ=60)
                                                        AAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCA         >  SRR18135085.9077135/1‑151 (MQ=60)
                                                        AAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCA         >  SRR18135085.280433/1‑151 (MQ=60)
                                                        AAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCA         >  SRR18135085.6197262/1‑151 (MQ=60)
                                                          GACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATC       >  SRR18135085.2227684/1‑151 (MQ=60)
                                                           ACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCC      >  SRR18135085.7903750/1‑151 (MQ=60)
                                                           ACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCC      >  SRR18135085.1797911/1‑151 (MQ=60)
                                                             CATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTC    <  SRR18135085.4654908/151‑1 (MQ=60)
                                                              ATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTCA   <  SRR18135085.6879006/151‑1 (MQ=60)
                                                              ATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTCA   >  SRR18135085.7860601/1‑151 (MQ=60)
                                                               TCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTCAT  >  SRR18135085.2640971/1‑151 (MQ=60)
                                                                |                                                                                                                                                     
ACCAGGACATTGAGGTTGAGAACGACGAGAGCCATCGGGTCGGCCACGACCGGACCAAGACCATTGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCACCGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTCAT  >  NC_008463/2545599‑2545812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑