Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 259 502.6 21734322 99.3% 21582181 151.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,947,654 2 bp→GG coding (468‑469/1416 nt) PA14_RS22670 → DNA polymerase Y family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,947,6540TG100.0% 1134.5 / NA 300I156M (ATT→CTG) PA14_RS22670DNA polymerase Y family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (168/132);  total (168/132)
*NC_0084634,947,6550TG100.0% 1136.2 / NA 300S157A (TCC→GCC) PA14_RS22670DNA polymerase Y family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (168/132);  total (168/132)

Only 100 of 300 total aligned reads displayed.

CCCTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGT‑CAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCG  >  NC_008463/4947521‑4947761
                                                                                                                                       ||                                                                                                          
cccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGAc                                                                                            <  2:10128039/151‑1 (MQ=255)
cccAGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGAc                                                                                            <  2:5897194/151‑1 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           >  1:8665801/1‑151 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           >  1:10228832/1‑151 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           <  2:10824748/151‑1 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           >  2:125213/1‑151 (MQ=255)
 ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                           >  1:4748247/1‑151 (MQ=255)
  cTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAg                                                                                          >  2:9256204/1‑151 (MQ=255)
  cTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAg                                                                                          <  2:9065650/151‑1 (MQ=255)
   tGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgc                                                                                         >  2:2559198/1‑151 (MQ=255)
   tGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgc                                                                                         <  2:5981362/151‑1 (MQ=255)
    gTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgcg                                                                                        >  1:4500882/1‑151 (MQ=255)
       cGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTg                                                                                     <  1:3124110/151‑1 (MQ=255)
        ggCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGc                                                                                    >  2:9012509/1‑151 (MQ=255)
          ccccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCaa                                                                                  >  2:9759575/1‑151 (MQ=255)
           cccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAac                                                                                 >  1:416539/1‑151 (MQ=255)
           cccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAac                                                                                 >  2:1135568/1‑151 (MQ=255)
            ccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAacg                                                                                >  2:2938266/1‑150 (MQ=255)
             cTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTNCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAacgc                                                                               >  1:5682797/1‑149 (MQ=255)
             cTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAacgc                                                                               >  1:6217870/1‑149 (MQ=255)
               ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCACCGCCa                                                                             >  2:8608428/1‑151 (MQ=255)
               ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                             <  1:9390003/151‑1 (MQ=255)
               ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                             >  1:6396214/1‑151 (MQ=255)
                gCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAg                                                                            >  1:3970373/1‑151 (MQ=255)
                 ccACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACCGCGCTGCACCGCCAGt                                                                           >  2:8233178/1‑151 (MQ=255)
                 ccACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGt                                                                           >  1:6211356/1‑151 (MQ=255)
                  cACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTACCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGtt                                                                          <  2:7900017/151‑1 (MQ=255)
                   aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                         <  1:4389440/151‑1 (MQ=255)
                   aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCGGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTg                                                                         <  1:3841434/151‑1 (MQ=255)
                    cGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGt                                                                        >  1:5082328/1‑151 (MQ=255)
                     gCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTc                                                                       >  2:6430079/1‑151 (MQ=255)
                      cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                      <  2:7376278/151‑1 (MQ=255)
                      cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCCGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGGGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                      <  2:1866111/151‑1 (MQ=255)
                         cGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                   <  1:10160988/151‑1 (MQ=255)
                         cGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                   >  1:7787941/1‑151 (MQ=255)
                          gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                  >  2:8114579/1‑151 (MQ=255)
                           aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                 <  2:4352492/151‑1 (MQ=255)
                           aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCt                                                                 <  2:3566960/151‑1 (MQ=255)
                            ggCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                                >  1:1558466/1‑151 (MQ=255)
                             gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                               >  1:6956471/1‑151 (MQ=255)
                               ccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCg                                                             <  1:7366859/151‑1 (MQ=255)
                                 gCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCt                                                           >  2:2134782/1‑151 (MQ=255)
                                 gCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCt                                                           >  1:9948227/1‑151 (MQ=255)
                                  cTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTg                                                          <  1:8377400/151‑1 (MQ=255)
                                  cTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTg                                                          >  2:5088252/1‑151 (MQ=255)
                                     gcgcACGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAc                                                       <  1:5334998/151‑1 (MQ=255)
                                     gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAc                                                       <  2:7789669/151‑1 (MQ=255)
                                     gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAc                                                       <  2:4501747/151‑1 (MQ=255)
                                      cgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                      <  2:10629004/151‑1 (MQ=255)
                                       gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCg                                                     <  2:40784/151‑1 (MQ=255)
                                           ggAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACCGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                 >  2:4833893/1‑151 (MQ=255)
                                            gAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTgg                                                >  2:2607764/1‑151 (MQ=255)
                                             aaCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTggg                                               <  1:3450427/151‑1 (MQ=255)
                                             aaCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGGGGCGGCGAGGGT‑CCTGGCGCAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTggg                                               <  2:1376905/151‑1 (MQ=255)
                                              aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                              >  1:9256436/1‑151 (MQ=255)
                                              aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                              <  2:4735214/151‑1 (MQ=255)
                                              aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                              >  2:7933656/1‑151 (MQ=255)
                                               cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                             <  2:6043198/151‑1 (MQ=255)
                                               cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                             >  1:3563821/1‑151 (MQ=255)
                                                  aCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActct                                          <  2:3915680/151‑1 (MQ=255)
                                                   cGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACtctc                                         <  1:6731771/151‑1 (MQ=255)
                                                      cgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                      >  2:518323/1‑151 (MQ=255)
                                                      cgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                      >  1:2709793/1‑151 (MQ=255)
                                                      cgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                      >  2:3616272/1‑151 (MQ=255)
                                                      cgcTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGAGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgcg                                      >  2:5758375/1‑151 (MQ=255)
                                                        cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCggg                                    <  2:1677578/151‑1 (MQ=255)
                                                        cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCT‑GGCGAAAAGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTAGCggg                                    >  2:8424940/1‑151 (MQ=255)
                                                           ggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGc                                 <  1:8267944/151‑1 (MQ=255)
                                                           ggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGc                                 <  2:10362853/151‑1 (MQ=255)
                                                           ggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGc                                 >  2:2220879/1‑151 (MQ=255)
                                                           cgCTACCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGc                                 <  2:6394943/150‑1 (MQ=255)
                                                            gcgtCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCGGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCt                                <  2:7195048/148‑1 (MQ=255)
                                                            gCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCt                                >  1:2249174/1‑151 (MQ=255)
                                                             cTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTg                               <  2:2829334/151‑1 (MQ=255)
                                                              ttCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTgg                              <  2:5535987/151‑1 (MQ=255)
                                                               tCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                             >  1:8406004/1‑151 (MQ=255)
                                                                  gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                          >  1:8552168/1‑151 (MQ=255)
                                                                   ccATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGCCGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTc                         <  2:272610/151‑1 (MQ=255)
                                                                      tCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGcc                      >  2:9342572/1‑151 (MQ=255)
                                                                        gCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                    <  2:1883535/151‑1 (MQ=255)
                                                                         ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                   <  1:5898710/151‑1 (MQ=255)
                                                                         ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                   <  1:7146537/151‑1 (MQ=255)
                                                                         ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                   <  1:9099349/151‑1 (MQ=255)
                                                                          cTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                  >  2:6667409/1‑151 (MQ=255)
                                                                          cTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                  <  1:1070177/151‑1 (MQ=255)
                                                                          cTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCg                  >  1:1989016/1‑151 (MQ=255)
                                                                           tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                 >  2:3255697/1‑151 (MQ=255)
                                                                           tgtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCgg                 <  2:6867374/151‑1 (MQ=255)
                                                                            gtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                >  1:10015802/1‑151 (MQ=255)
                                                                            gtgCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGa                >  1:7910648/1‑151 (MQ=255)
                                                                             tgCCTGGCGCCGAACCCGGAGGCGGCGAGGGTCCGG‑GCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGCGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc               <  2:8808169/151‑1 (MQ=255)
                                                                                   gcgcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATgg         <  1:8615081/151‑1 (MQ=255)
                                                                                    cgcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATggg        <  2:10266365/151‑1 (MQ=255)
                                                                                    cgcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATggg        <  2:2166690/151‑1 (MQ=255)
                                                                                     gcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGa       >  1:1221387/1‑151 (MQ=255)
                                                                                     gcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGa       <  2:9748074/151‑1 (MQ=255)
                                                                                       cGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACt     >  1:3302987/1‑151 (MQ=255)
                                                                                        gAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTg    >  2:7548974/1‑151 (MQ=255)
                                                                                         aaCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc   <  1:1280312/151‑1 (MQ=255)
                                                                                         aaCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc   <  2:1608004/151‑1 (MQ=255)
                                                                                                                                       ||                                                                                                          
CCCTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGT‑CAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGCG  >  NC_008463/4947521‑4947761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTC  >  NC_008463/4947546‑4947738
                                                                                                             |                                                                                    
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                            <  SRR18135085.10196763/151‑1 (MQ=60)
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                            >  SRR18135085.7002909/1‑151 (MQ=60)
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                            >  SRR18135085.9957206/1‑151 (MQ=60)
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                            >  SRR18135085.7816365/1‑151 (MQ=60)
 GAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCC                                           <  SRR18135085.4726571/151‑1 (MQ=60)
 GAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCC                                           >  SRR18135085.106318/1‑151 (MQ=60)
 GAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCC                                           >  SRR18135085.8676146/1‑151 (MQ=60)
 GAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCAGCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCC                                           >  SRR18135085.4998806/1‑151 (MQ=60)
  AGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCT                                          <  SRR18135085.4334270/151‑1 (MQ=60)
  AGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCT                                          >  SRR18135085.2118274/1‑151 (MQ=60)
   GGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTT                                         >  SRR18135085.1564622/1‑151 (MQ=60)
    GCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTC                                        >  SRR18135085.6982347/1‑151 (MQ=60)
     CCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCC                                       <  SRR18135085.1230123/151‑1 (MQ=60)
      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                      <  SRR18135085.8536517/151‑1 (MQ=60)
      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                      <  SRR18135085.7393972/151‑1 (MQ=60)
      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                      >  SRR18135085.285311/1‑151 (MQ=60)
      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                      >  SRR18135085.3154075/1‑151 (MQ=60)
        GCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCT                                    >  SRR18135085.1222951/1‑151 (MQ=60)
        GCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCT                                    >  SRR18135085.9983262/1‑151 (MQ=60)
         CTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTG                                   <  SRR18135085.8417184/151‑1 (MQ=60)
         CTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTG                                   >  SRR18135085.5030525/1‑151 (MQ=60)
         CTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTG                                   <  SRR18135085.8407669/151‑1 (MQ=60)
          TTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGG                                  <  SRR18135085.3505013/151‑1 (MQ=60)
            GCGCACGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                <  SRR18135085.5355835/151‑1 (MQ=60)
            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                >  SRR18135085.7298238/1‑151 (MQ=60)
             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                               >  SRR18135085.1736507/1‑151 (MQ=60)
               CAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGC                             >  SRR18135085.9509557/1‑151 (MQ=60)
                AAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCC                            >  SRR18135085.3480016/1‑151 (MQ=60)
                AAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCC                            >  SRR18135085.8681934/1‑151 (MQ=60)
                AAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCC                            >  SRR18135085.6239302/1‑151 (MQ=60)
                   GAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGG                         <  SRR18135085.6124466/151‑1 (MQ=60)
                    AACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGG                        <  SRR18135085.3463802/151‑1 (MQ=60)
                    AACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGG                        >  SRR18135085.3383054/1‑151 (MQ=60)
                     ACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGA                       >  SRR18135085.9289473/1‑151 (MQ=60)
                     ACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGA                       >  SRR18135085.9289651/1‑151 (MQ=60)
                      CTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAC                      >  SRR18135085.3577583/1‑151 (MQ=60)
                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                   <  SRR18135085.1312618/151‑1 (MQ=60)
                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                   <  SRR18135085.8515165/151‑1 (MQ=60)
                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                  <  SRR18135085.6757023/151‑1 (MQ=60)
                           GGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCG                 <  SRR18135085.1736342/151‑1 (MQ=60)
                             CGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCG               <  SRR18135085.7934789/151‑1 (MQ=60)
                             CGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCG               >  SRR18135085.2720302/1‑151 (MQ=60)
                                TGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA            >  SRR18135085.1019511/1‑151 (MQ=60)
                                  GGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGC          <  SRR18135085.8297803/151‑1 (MQ=60)
                                  GGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGC          >  SRR18135085.998958/1‑151 (MQ=60)
                                   GCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCT         >  SRR18135085.2257938/1‑151 (MQ=60)
                                   GCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCGGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCT         <  SRR18135085.7825825/151‑1 (MQ=60)
                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC      >  SRR18135085.8436076/1‑151 (MQ=60)
                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC      >  SRR18135085.8436372/1‑151 (MQ=60)
                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC      >  SRR18135085.5277048/1‑151 (MQ=60)
                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC      >  SRR18135085.2327217/1‑151 (MQ=60)
                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC     >  SRR18135085.10100556/1‑151 (MQ=60)
                                        CGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCG    >  SRR18135085.4503162/1‑151 (MQ=60)
                                        CGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCG    >  SRR18135085.3015870/1‑151 (MQ=60)
                                         GCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGT   >  SRR18135085.8113682/1‑151 (MQ=60)
                                         GCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGT   >  SRR18135085.8582987/1‑151 (MQ=60)
                                          CCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTC  >  SRR18135085.6274586/1‑151 (MQ=60)
                                                                                                             |                                                                                    
CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTC  >  NC_008463/4947546‑4947738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑