Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	275	500.0	21685688	99.2%	21512202	151.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,809,318	G→C	intergenic (‑204/+15)	rpsF ← / ← rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,809,318	0	G	C	99.7%	1279.4 / NA	346	intergenic (‑204/+15)	rpsF/rlmB	30S ribosomal protein S6/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (202/142); minor base A (1/0); total (204/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

TGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC  >  NC_008463/5809176‑5809462

tcttggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCa                                                                                                                                         >  1:887526‑M2/124‑151 (MQ=255)

   tggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAcgc                                                                                                                                      >  1:10580410‑M2/121‑151 (MQ=255)

    ggcttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCCCAcgcg                                                                                                                                     >  1:923501‑M2/120‑151 (MQ=255)

      cttgcctggtggagtggcgaactaacgcctgcccgcgcggcacggggctacatactagagaagggcaaccggcgacgcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCt                                                                                                                                   <  1:3939278‑M2/34‑1 (MQ=255)

       ttgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTc                                                                                                                                  <  1:7538913‑M2/35‑1 (MQ=255)

        tgcctggtggagaggcgaacgaccgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                 <  2:440874‑M2/36‑1 (MQ=255)

        tgcctggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattcgagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCa                                                                                                                                 >  1:111907‑M2/116‑151 (MQ=255)

            tggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagacgggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTcc                                                                                                                             >  2:8359064‑M2/112‑151 (MQ=255)

             ggtggagaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCa                                                                                                                            >  1:10042940‑M2/111‑151 (MQ=255)

                 gagaggcgaacgaccgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgg                                                                                                                        <  2:2053381‑M2/45‑1 (MQ=255)

                  agaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCggg                                                                                                                       <  2:6799975‑M2/46‑1 (MQ=255)

                   gaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                      >  1:5488659‑M2/105‑151 (MQ=255)

                   gaggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCgggg                                                                                                                      >  2:4232450‑M2/105‑151 (MQ=255)

                    aggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                     <  2:2767506‑M2/48‑1 (MQ=255)

                    aggcgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGt                                                                                                                     <  2:4013765‑M2/48‑1 (MQ=255)

                       cgaacgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGTGCTCACTCCAGCGGGGTgcg                                                                                                                  <  1:8488074‑M2/51‑1 (MQ=255)

                           cgaacgcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatagttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCCCTCCAGCGGGGTGCGCTgg                                                                                                              >  2:389607‑M2/97‑151 (MQ=255)

                                gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                         >  2:5603020‑M2/92‑151 (MQ=255)

                                gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                         >  1:6005511‑M2/92‑151 (MQ=255)

                                gcctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggag                                                                                                         >  1:1686545‑M2/92‑151 (MQ=255)

                                 cctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggagg                                                                                                        >  2:7401849‑M2/91‑151 (MQ=255)

                                  ctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCggagga                                                                                                       >  1:805088‑M2/90‑151 (MQ=255)

                                  ctgcccgcgcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCGGGcggaggt                                                                                                       >  1:9952916‑M2/90‑150 (MQ=255)

                                          gcggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAAc                                                                                               >  2:9613099‑M2/82‑151 (MQ=255)

                                           cggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACa                                                                                              >  1:5274770‑M2/81‑151 (MQ=255)

                                           cggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGACGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACa                                                                                              >  2:2884692‑M2/81‑151 (MQ=255)

                                            ggcaaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAg                                                                                             >  2:3266007‑M2/80‑151 (MQ=255)

                                              caaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGc                                                                                           <  2:10806077‑M2/74‑1 (MQ=255)

                                               aaggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCa                                                                                          >  2:9098071‑M2/77‑151 (MQ=255)

                                                aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                         <  2:4285065‑M2/76‑1 (MQ=255)

                                                aggggcgacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAg                                                                                         <  1:1802568‑M2/76‑1 (MQ=255)

                                                 ggggcgacattcgagagaagggcaaccggcgaagcaaggaagattggcgaagatttgtacagcggagggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCAGGCGGAGGCTCTCGAACAGGCAGa                                                                                        >  2:486536‑M2/75‑151 (MQ=255)

                                                      gacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                   <  2:8577609‑M2/82‑1 (MQ=255)

                                                      gacattctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCg                                                                                   <  2:6424277‑M2/82‑1 (MQ=255)

                                                       acattctagagaagggcaaccggcgaagcaaggaagattggcgactatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCgg                                                                                  <  2:6090528‑M2/83‑1 (MQ=255)

                                                          ttctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTgg                                                                               >  1:3306970‑M2/66‑151 (MQ=255)

                                                            ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtaccgcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                             <  2:666677‑M2/88‑1 (MQ=255)

                                                            ctagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCg                                                                             <  2:127265‑M2/88‑1 (MQ=255)

                                                             tagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGa                                                                            >  1:7271711‑M2/63‑151 (MQ=255)

                                                             tagagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGa                                                                            >  1:9712594‑M2/63‑151 (MQ=255)

                                                              agagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAc                                                                           >  2:4360545‑M2/62‑151 (MQ=255)

                                                               gagaagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGC‑GGGTCCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGACGCCGGTGGCGAcc                                                                          >  1:2091506‑M2/61‑151 (MQ=255)

                                                                  aagggcaaccggcgaagcaaggaagattggcgaatatttgtccagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgag                                                                       <  2:9163699‑M2/94‑1 (MQ=255)

                                                                  aagggcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCgag                                                                       <  1:5719690‑M2/94‑1 (MQ=255)

                                                                     ggcaaccggcgaagcaaggaagattggcgaatatttggacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACg                                                                    <  2:5166936‑M2/97‑1 (MQ=255)

                                                                      gcaaccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGt                                                                   <  2:3066413‑M2/98‑1 (MQ=255)

                                                                       caaccggcgaagcaaggaagattggcgaatctttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGtt                                                                  <  2:8231303‑M2/99‑1 (MQ=255)

                                                                          ccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAg                                                               <  1:2777701‑M2/102‑1 (MQ=255)

                                                                          ccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAg                                                               >  1:4279527‑M2/50‑151 (MQ=255)

                                                                          ccggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGAGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTTGCGACCGAGACGTTCAg                                                               >  2:1039644‑M2/50‑151 (MQ=255)

                                                                           cggcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCCGCCCCCCACCCGCTCACGCCAGCGCGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACCTTCAgg                                                              >  1:4718431‑M2/49‑151 (MQ=255)

                                                                            ggcgaagcaaggaagattggcgaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCCGACGCCGGTGGCGACCGAGCCGTTCAGgc                                                             >  2:8398495‑M2/48‑151 (MQ=255)

                                                                             gcgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgct                                                            <  1:2686110‑M2/105‑1 (MQ=255)

                                                                              cgaagcaaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGgctg                                                           >  2:8078406‑M2/46‑151 (MQ=255)

                                                                                    aaggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACg                                                     <  2:1226140‑M2/112‑1 (MQ=255)

                                                                                      ggaagattggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGAGCGAGACGTTCAGGCTGCTGACGCt                                                   >  2:3528076‑M2/38‑151 (MQ=255)

                                                                                            ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCAGCCGGc                                             >  1:1366630‑M2/32‑151 (MQ=255)

                                                                                            ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAAGCTGCCGGc                                             >  1:6379627‑M2/32‑151 (MQ=255)

                                                                                            ttggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                             >  1:7192556‑M2/32‑151 (MQ=255)

                                                                                            ttggcgaatattagtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGc                                             <  1:4113565‑M2/120‑1 (MQ=255)

                                                                                              ggcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCa                                           >  2:8978609‑M2/30‑151 (MQ=255)

                                                                                               gcgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAt                                          >  1:8406897‑M2/29‑151 (MQ=255)

                                                                                                cgaatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATc                                         >  2:1119276‑M2/28‑151 (MQ=255)

                                                                                                 gaatatttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCg                                        <  1:2332987‑M2/125‑1 (MQ=255)

                                                                                                  aatatttgtacagcggatggaatggCGCGGGGCCGCGCCC‑CGGCGGCCCCGCACGCGCACACACCAGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGCGACGTTCAGGCTGCTGACGCTGCCGGCCCTCgg                                       >  1:3035641‑M2/26‑151 (MQ=255)

                                                                                                  aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                       >  2:5842240‑M2/26‑151 (MQ=255)

                                                                                                  aatatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCgg                                       >  2:10576700‑M2/26‑151 (MQ=255)

                                                                                                   atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                      <  1:9572715‑M2/127‑1 (MQ=255)

                                                                                                   atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGc                                      <  1:5306269‑M2/127‑1 (MQ=255)

                                                                                                   atatttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCGGCTGACGCTGCCGGCCATCGGc                                      >  2:2218278‑M2/25‑151 (MQ=255)

                                                                                                     atttgtacagcggatggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGCCGCTGCCGGCCATCGGCaa                                    <  2:2454678‑M2/129‑1 (MQ=255)

                                                                                                     atttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGGCaa                                    >  2:10184611‑M2/23‑151 (MQ=255)

                                                                                                     atttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCaa                                    <  2:8674682‑M2/129‑1 (MQ=255)

                                                                                                      tttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAc                                   >  2:6339390‑M2/22‑151 (MQ=255)

                                                                                                       ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAcc                                  <  1:3829847‑M2/131‑1 (MQ=255)

                                                                                                       ttgtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAAcc                                  <  1:9784327‑M2/131‑1 (MQ=255)

                                                                                                         gtacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTg                                >  2:5468544‑M2/19‑151 (MQ=255)

                                                                                                          tacagcggatggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTgg                               <  1:4377193‑M2/134‑1 (MQ=255)

                                                                                                                  atggactggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                       <  2:9222544‑M2/142‑1 (MQ=255)

                                                                                                                  atggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCAGGCGGAGGATATCGAACAGGCAGAAGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAg                       >  1:5257911‑M2/10‑151 (MQ=255)

                                                                                                                   tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                      <  1:2875453‑M2/143‑1 (MQ=255)

                                                                                                                   tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGATCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                      >  2:1748576‑M2/9‑151 (MQ=255)

                                                                                                                   tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGAAGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGt                      >  2:4106505‑M2/9‑151 (MQ=255)

                                                                                                                   tggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCAAGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCGGGCGAGGTagg                      >  1:5644599‑M2/9‑150 (MQ=255)

                                                                                                                    ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGCGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGTAGTc                     >  2:7649466‑M2/8‑151 (MQ=255)

                                                                                                                    ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGCACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTc                     >  2:3132323‑M2/8‑151 (MQ=255)

                                                                                                                    ggaatggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGTGACGTTCAGGCTGCTGACGCTGCCGGCCAACGGCAACCTGGCGAGGAAGTc                     >  1:10270801‑M2/8‑151 (MQ=255)

                                                                                                                      cagggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGCCCAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGc                   <  2:7038005‑M2/146‑1 (MQ=255)

                                                                                                                       atggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCa                  <  1:6913560‑M2/147‑1 (MQ=255)

                                                                                                                         ggCGCGGGG‑CCGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGt                <  2:2390061‑M2/149‑1 (MQ=255)

                                                                                                                            gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGACGTCGCAGTGcg             >  2:9758167/1‑150 (MQ=255)

                                                                                                                            gcgGGGCCGCG‑CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGGTCAGGCTGCTGACGCTGCCGGCCATCGGCACCCTGGCGAGGAAGTCGCAGTGCt             >  2:8208696/1‑151 (MQ=255)

                                                                                                                                 ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg         <  2:7965434/144‑1 (MQ=255)

                                                                                                                                 ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg         <  2:264867/144‑1 (MQ=255)

                                                                                                                                 ggccgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTcgcg         <  1:7818015/144‑1 (MQ=255)

                                                                                                                                    cgcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGt      >  2:4646599/5‑151 (MQ=255)

                                                                                                                                     gcgccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTc     <  1:6281390/148‑1 (MQ=255)

                                                                                                                                      cgccGCGGCGGCCCCGCACGCGCTAACTCCAGAGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGACGGCCATCGGCAACCTGGCGAGGAAGACGCAGTGCTCGCGGGTCa    >  2:6220099/3‑151 (MQ=255)

                                                                                                                                        ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGCTCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAGCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGc  >  2:10483871/1‑151 (MQ=255)

                                                                                                                                        ccGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGc  >  1:6742320/1‑151 (MQ=255)

TGGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCCCCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC  >  NC_008463/5809176‑5809462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCC‑CCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGC  >  NC_008463/5809177‑5809468

GGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCG                                                                                                                                           >  SRR18135068.10236064/1‑151 (MQ=60)

 GCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGC                                                                                                                                          >  SRR18135068.5692013/1‑151 (MQ=60)

  CTTGCCTGGTGGAGTGGCGAACTAACGCCTGCCCGCGCGGCACGGGGCTACATACTAGAGAAGGGCAACCGGCGACGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCT                                                                                                                                         <  SRR18135068.3958895/151‑1 (MQ=60)

   TTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGACGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTC                                                                                                                                        <  SRR18135068.2123063/151‑1 (MQ=60)

   TTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTC                                                                                                                                        <  SRR18135068.7575132/151‑1 (MQ=60)

    TGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCA                                                                                                                                       >  SRR18135068.8318197/1‑151 (MQ=60)

    TGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCGAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCA                                                                                                                                       >  SRR18135068.112513/1‑151 (MQ=60)

      CCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACT                                                                                                                                     >  SRR18135068.8507941/1‑151 (MQ=60)

       CTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTC                                                                                                                                    >  SRR18135068.7872692/1‑151 (MQ=60)

         GGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCA                                                                                                                                  >  SRR18135068.10090007/1‑151 (MQ=60)

         GGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCA                                                                                                                                  >  SRR18135068.527876/1‑151 (MQ=60)

         GGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCGCACTCCA                                                                                                                                  >  SRR18135068.8381744/1‑151 (MQ=60)

          GTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAG                                                                                                                                 >  SRR18135068.6041669/1‑151 (MQ=60)

          GTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAG                                                                                                                                 <  SRR18135068.10730279/151‑1 (MQ=60)

           TGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGC                                                                                                                                >  SRR18135068.3876411/1‑151 (MQ=60)

           TGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGC                                                                                                                                >  SRR18135068.3876445/1‑151 (MQ=60)

               GAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGG                                                                                                                            >  SRR18135068.5516777/1‑151 (MQ=60)

               GAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGG                                                                                                                            >  SRR18135068.5516032/1‑151 (MQ=60)

                AGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGT                                                                                                                           >  SRR18135068.4184672/1‑151 (MQ=60)

                  GCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGC                                                                                                                         <  SRR18135068.5897763/151‑1 (MQ=60)

                   CGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGTGCTCACTCCAGCGGGGTGCG                                                                                                                        <  SRR18135068.8528408/151‑1 (MQ=60)

                   CGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCG                                                                                                                        >  SRR18135068.10362323/1‑151 (MQ=60)

                    GAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGC                                                                                                                       >  SRR18135068.3729565/1‑151 (MQ=60)

                     AACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCT                                                                                                                      <  SRR18135068.2081964/151‑1 (MQ=60)

                     AACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCT                                                                                                                      >  SRR18135068.6317772/1‑151 (MQ=60)

                      ACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTG                                                                                                                     >  SRR18135068.1990041/1‑151 (MQ=60)

                       CGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAAGATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGG                                                                                                                    >  SRR18135068.4511114/1‑151 (MQ=60)

                        GACCGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATAGTTGTACAGCGGAGGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGG                                                                                                                   >  SRR18135068.4564326/1‑151 (MQ=60)

                        GAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGC                                                                                                                   >  SRR18135068.9502443/1‑151 (MQ=60)

                          ACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGG                                                                                                                 >  SRR18135068.6820647/1‑151 (MQ=60)

                           CGCCTGCCCGCGCGGCAAGGGGCGACATGCTAGAGAAGGGCAACCGGCGACGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGA                                                                                                                <  SRR18135068.5633899/151‑1 (MQ=60)

                            GCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGTATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAG                                                                                                               >  SRR18135068.6035237/1‑151 (MQ=60)

                            GCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAG                                                                                                               >  SRR18135068.6035245/1‑151 (MQ=60)

                            GCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAG                                                                                                               >  SRR18135068.9727332/1‑151 (MQ=60)

                            GCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGAGGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAG                                                                                                               >  SRR18135068.1695075/1‑151 (MQ=60)

                             CCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGCAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTCCAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGG                                                                                                              <  SRR18135068.10713893/151‑1 (MQ=60)

                             CCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGG                                                                                                              <  SRR18135068.5410414/151‑1 (MQ=60)

                              CTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGA                                                                                                             <  SRR18135068.5535578/151‑1 (MQ=60)

                              CTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGA                                                                                                             >  SRR18135068.2605579/1‑151 (MQ=60)

                              CTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGA                                                                                                             >  SRR18135068.808481/1‑151 (MQ=60)

                              CTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGA                                                                                                             >  SRR18135068.809263/1‑151 (MQ=60)

                              CTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGA                                                                                                             >  SRR18135068.1360554/1‑151 (MQ=60)

                              CTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCGGGCGGAGGT                                                                                                             >  SRR18135068.9999528/1‑151 (MQ=60)

                               TGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGAT                                                                                                            <  SRR18135068.8476499/151‑1 (MQ=60)

                                       CGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACA                                                                                                    >  SRR18135068.5300961/1‑151 (MQ=60)

                                       CGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACA                                                                                                    >  SRR18135068.5301045/1‑151 (MQ=60)

                                       CGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGATCA                                                                                                    >  SRR18135068.1247257/1‑151 (MQ=60)

                                          CAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGC                                                                                                 <  SRR18135068.6237531/151‑1 (MQ=60)

                                           AAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCA                                                                                                <  SRR18135068.3993528/151‑1 (MQ=60)

                                           AAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCA                                                                                                >  SRR18135068.5320901/1‑151 (MQ=60)

                                           AAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCACACACCAGCGGGGTGCGCTGGCGGAGGATATCGAACAGGCA                                                                                                >  SRR18135068.5318923/1‑151 (MQ=60)

                                            AGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAG                                                                                               <  SRR18135068.1811617/151‑1 (MQ=60)

                                            AGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAG                                                                                               >  SRR18135068.2800568/1‑151 (MQ=60)

                                                GCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGC                                                                                           >  SRR18135068.1837667/1‑151 (MQ=60)

                                                  GACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCG                                                                                         >  SRR18135068.3102010/1‑151 (MQ=60)

                                                     ATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGTTTGGCGAATATTTGTACAGCGGAGGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATATCGAACAGGCAGACGCCGGTG                                                                                      <  SRR18135068.1592405/151‑1 (MQ=60)

                                                     ATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTG                                                                                      <  SRR18135068.9583292/151‑1 (MQ=60)

                                                      TTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGG                                                                                     >  SRR18135068.3323456/1‑151 (MQ=60)

                                                         TAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGA                                                                                  >  SRR18135068.9758218/1‑151 (MQ=60)

                                                         TAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGA                                                                                  >  SRR18135068.9764271/1‑151 (MQ=60)

                                                         TAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGA                                                                                  >  SRR18135068.7306710/1‑151 (MQ=60)

                                                           GAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGC‑GGGTC‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGACGCCGGTGGCGACC                                                                                >  SRR18135068.2102135/1‑151 (MQ=60)

                                                            AGACGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCG                                                                               <  SRR18135068.10853817/151‑1 (MQ=60)

                                                            AGAAGGGCAACCGGCGAAGCAAGGAAGTTGGGCGAATTTTTGTACAGCGTATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCG                                                                               <  SRR18135068.10606326/151‑1 (MQ=60)

                                                            AGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCG                                                                               >  SRR18135068.1698750/1‑151 (MQ=60)

                                                            AGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCG                                                                               >  SRR18135068.3387030/1‑151 (MQ=60)

                                                            AGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCG                                                                               >  SRR18135068.1694342/1‑151 (MQ=60)

                                                              AAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAG                                                                             <  SRR18135068.5748029/151‑1 (MQ=60)

                                                              AAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAG                                                                             <  SRR18135068.5748157/151‑1 (MQ=60)

                                                               AGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGA                                                                            <  SRR18135068.2593653/151‑1 (MQ=60)

                                                               AGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGA                                                                            >  SRR18135068.741062/1‑151 (MQ=60)

                                                                GGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGAC                                                                           >  SRR18135068.4332670/1‑151 (MQ=60)

                                                                    AACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATATCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTC                                                                       >  SRR18135068.2219681/1‑151 (MQ=60)

                                                                     ACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCCCGCGCTCACTCCAGCGGGGTGCGCTGGCGGTGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCA                                                                      >  SRR18135068.7322555/1‑151 (MQ=60)

                                                                      CCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACCGCGGAGGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAG                                                                     <  SRR18135068.5491092/151‑1 (MQ=60)

                                                                      CCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAG                                                                     <  SRR18135068.2791613/151‑1 (MQ=60)

                                                                      CCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAG                                                                     <  SRR18135068.2791609/151‑1 (MQ=60)

                                                                      CCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAG                                                                     <  SRR18135068.2788468/151‑1 (MQ=60)

                                                                      CCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAG                                                                     >  SRR18135068.4300781/1‑151 (MQ=60)

                                                                       CGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCCGCCCCCCACCCGCTCACGCCAGCGCGGTGCGCAGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACCTTCAGG                                                                    >  SRR18135068.4741946/1‑151 (MQ=60)

                                                                        GGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGC                                                                   <  SRR18135068.4311493/151‑1 (MQ=60)

                                                                         GCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCT                                                                  <  SRR18135068.2699511/151‑1 (MQ=60)

                                                                          CGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGGGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTG                                                                 >  SRR18135068.295209/1‑151 (MQ=60)

                                                                          CGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTG                                                                 >  SRR18135068.295164/1‑151 (MQ=60)

                                                                          CGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTG                                                                 >  SRR18135068.1418794/1‑151 (MQ=60)

                                                                            AAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCT                                                               >  SRR18135068.9796279/1‑151 (MQ=60)

                                                                            AAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACCCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGTACAGGCCGACGCCGGTGGCGACCGAGACGTTCAGGCTGCG                                                               >  SRR18135068.640104/1‑151 (MQ=60)

                                                                             AGCAAGGAAGATTGGCGAATTTTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTG                                                              <  SRR18135068.3136867/151‑1 (MQ=60)

                                                                             AGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCCGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTGAGGCTGCGG                                                              >  SRR18135068.4018144/1‑151 (MQ=60)

                                                                                AAGGACGATTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCCCTCCAGCGGGGTGCGCTGGCGGAGGATCTCGACCAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACG                                                           <  SRR18135068.700254/151‑1 (MQ=60)

                                                                                     AGATTGGCGAATATTTGTACAGCGGATGGCATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCC                                                      <  SRR18135068.6142865/151‑1 (MQ=60)

                                                                                        TTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGC                                                   >  SRR18135068.7227755/1‑151 (MQ=60)

                                                                                        TTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCAGCCGGC                                                   >  SRR18135068.1373694/1‑151 (MQ=60)

                                                                                        TTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGAAGCTGCCGGC                                                   >  SRR18135068.6410884/1‑151 (MQ=60)

                                                                                        TTGGCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACACCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGC                                                   >  SRR18135068.7227199/1‑151 (MQ=60)

                                                                                        TTGGCGAATATTAGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGC                                                   <  SRR18135068.4134018/151‑1 (MQ=60)

                                                                                          GGCGAATATTTGTACAGCGGATGGAA‑GGGCGC‑‑‑GGGGC‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCA                                                 <  SRR18135068.505678/151‑1 (MQ=60)

                                                                                           GCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAT                                                <  SRR18135068.3778064/151‑1 (MQ=60)

                                                                                           GCGAATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCGCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAT                                                >  SRR18135068.8446865/1‑151 (MQ=60)

                                                                                             GAATATTTGTACAGCGGATGGACTG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCG                                              <  SRR18135068.2344764/151‑1 (MQ=60)

                                                                                              AATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGGCCGCGCCCC‑‑GGCGGCCCCGCACGCGCACACACCAGCGGGGTGCGCGGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGCGACGTTCAGGCTGCTGACGCTGCCGGCCCTCGG                                             >  SRR18135068.3050805/1‑151 (MQ=60)

                                                                                              AATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGG                                             >  SRR18135068.7877484/1‑151 (MQ=60)

                                                                                               ATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGC                                            <  SRR18135068.9617758/151‑1 (MQ=60)

                                                                                               ATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGC                                            <  SRR18135068.5332694/151‑1 (MQ=60)

                                                                                               ATATTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGC                                            <  SRR18135068.7275464/151‑1 (MQ=60)

                                                                                                  TTTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCAACGGCAAC                                         >  SRR18135068.1750702/1‑151 (MQ=60)

                                                                                                   TTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACC                                        <  SRR18135068.3848934/151‑1 (MQ=60)

                                                                                                   TTGTACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACC                                        <  SRR18135068.9830243/151‑1 (MQ=60)

                                                                                                      TACAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGG                                     <  SRR18135068.4398878/151‑1 (MQ=60)

                                                                                                        CAGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCG                                   >  SRR18135068.9882963/1‑151 (MQ=60)

                                                                                                         AGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATATCGAACAGGCAGACGCCGGTGGCGACCGCGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGA                                  >  SRR18135068.7786918/1‑151 (MQ=60)

                                                                                                         AGCGGATGGAATG‑‑‑GCGCGGGG‑C‑CGCGCCGCGGCGGCCCCGCACGCGCACACTACAGCGGGGTGCGCTGGCGGAGGATATCGAAAAGGCAGACGCCGGTGGCGACCGATACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGA                                  >  SRR18135068.7786525/1‑151 (MQ=60)

                                                                                                                                        CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC        >  SRR18135068.10173906/1‑151 (MQ=60)

                                                                                                                                        CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC        >  SRR18135068.8514989/1‑151 (MQ=60)

                                                                                                                                        CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC        >  SRR18135068.6775201/1‑151 (MQ=60)

                                                                                                                                        CCGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGC        >  SRR18135068.664926/1‑151 (MQ=60)

                                                                                                                                         CGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCC       >  SRR18135068.7538196/1‑151 (MQ=60)

                                                                                                                                         CGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCC       >  SRR18135068.7530374/1‑151 (MQ=60)

                                                                                                                                         CGCGGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCC       >  SRR18135068.7530112/1‑151 (MQ=60)

                                                                                                                                            GGCGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGC    >  SRR18135068.8150398/1‑151 (MQ=60)

                                                                                                                                              CGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTCGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGC  >  SRR18135068.537538/1‑151 (MQ=60)

GGCTTGCCTGGTGGAGAGGCGAACGAACGCCTGCCCGCGCGGCAAGGGGCGACATTCTAGAGAAGGGCAACCGGCGAAGCAAGGAAGATTGGCGAATATTTGTACAGCGGATGGAATGGGCGCGCGGGGCC‑CCCCCCGGGGGGGCCCCGCACGCGCTCACTCCAGCGGGGTGCGCTGGCGGAGGATCTTGAACAGGCAGACGCCGGTGGCGACCGAGACGTTCAGGCTGCTGACGCTGCCGGCCATCGGCAACCTGGCGAGGAAGTCGCAGTGCTCGCGGGTCAGCCGGCGC  >  NC_008463/5809177‑5809468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`