Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 255 512.9 22386308 99.2% 22207217 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,515,863 T→C intergenic (+617/+267) PA14_RS16005 → / ← PA14_RS16010 AAA family ATPase/DUF4354 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,515,8630TC99.7% 1142.5 / NA 303intergenic (+617/+267)PA14_RS16005/PA14_RS16010AAA family ATPase/DUF4354 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (146/156);  minor base A (1/0);  total (147/156)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

TATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCTTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAA  >  NC_008463/3515716‑3516009
                                                                                                                                                    |                                                                                                                                                   
tataCAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTc                                                                                                                                                   <  2:5048484/150‑1 (MQ=255)
    cAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCTCAccc                                                                                                                                               >  2:10342387/1‑150 (MQ=255)
         cctccctAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCTCACCCCAAcc                                                                                                                                          >  2:10796006/1‑150 (MQ=255)
         cctccctAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCAAACCCCAAcc                                                                                                                                          >  2:9430987/1‑150 (MQ=255)
            ccctAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCctc                                                                                                                                       <  1:401862/150‑1 (MQ=255)
            ccctAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCACACCCCAAcccac                                                                                                                                       >  2:3378184/1‑148 (MQ=255)
              ctAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCtctc                                                                                                                                     <  2:8225739/150‑1 (MQ=255)
                   ccATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCCACCCACTCCCCCa                                                                                                                                >  2:2252015/1‑150 (MQ=255)
                    cATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCTCACCCCAAACCTCTccccccc                                                                                                                               >  1:9680194/1‑146 (MQ=255)
                    cATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCAc                                                                                                                               <  2:10919513/150‑1 (MQ=255)
                     aTAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCACACCCGCAcg                                                                                                                              >  2:8893590/1‑150 (MQ=255)
                      tAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTCACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTG‑GGGCCCAACCCCAAACCACTACCCCAcgc                                                                                                                             >  2:2014979/1‑150 (MQ=255)
                       aGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCGAACCGCAATAGCTGCGGATTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCAcgcg                                                                                                                            <  1:11071082/150‑1 (MQ=255)
                         cccTTCCCCATACCTGAACGGCACACCTCTTGCCACCCGCAAAACCACACCCGCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCggg                                                                                                                          <  1:3607755/150‑1 (MQ=255)
                              ccccATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑Agg                                                                                                                    <  2:9878359/150‑1 (MQ=255)
                              ccccATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑Agg                                                                                                                    <  1:1476333/150‑1 (MQ=255)
                                ccATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑Agggg                                                                                                                  <  1:10917817/150‑1 (MQ=255)
                                 cATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCGCACCCCAAACCACAACCCCACGCGGGCG‑gggggg                                                                                                                 >  1:6897377/1‑150 (MQ=255)
                                 cATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑Aggggg                                                                                                                 <  1:9540472/150‑1 (MQ=255)
                                   tACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCACAACCGCACCCCGGAG‑Tggggggc                                                                                                               >  1:6210850/1‑148 (MQ=255)
                                      cTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCggg                                                                                                            <  2:7922398/150‑1 (MQ=255)
                                       tGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCACACCCCCACCCACTCCCGCACGCGGGGG‑TTGGGGGCgggg                                                                                                           >  1:4870610/1‑149 (MQ=255)
                                        gAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCTCACCCCAACCCGCGCCCGCACCCGGGAG‑TGGGGggcgggcg                                                                                                          >  1:4198483/1‑150 (MQ=255)
                                          ccGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGACTGTGGTGTT‑GGCCCTCACCCCAAACCACAACCGCACCCGGGAG‑AGGGGGCCGGGCGTg                                                                                                        >  2:3971164/2‑150 (MQ=255)
                                           cGGCACACCTATTGCCACCCGCAAAACCACCCCCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAAGGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGc                                                                                                       <  2:7329473/150‑1 (MQ=255)
                                           cGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCTCACCCCAACCCACAACCGCACGCGGGAG‑AGGGGGCCGGGCGTGc                                                                                                       >  2:5622178/1‑150 (MQ=255)
                                             gCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCACACCCCAACCCTCAACCGCACGCGGGAG‑CGGGGGCCGGGCGGGccg                                                                                                     >  2:9819293/1‑149 (MQ=255)
                                              cacaCCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCACACCCCAAACCACTACCCCCCGCCGGAG‑AGGGGGCCGGGCGTggcgg                                                                                                    >  2:2121036/1‑146 (MQ=255)
                                                cacCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTtgt                                                                                                  <  2:8594804/150‑1 (MQ=255)
                                                 acCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAAACCACTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCGtgtg                                                                                                 >  2:6895825/1‑150 (MQ=255)
                                                  ccTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTgg                                                                                                <  1:4561870/150‑1 (MQ=255)
                                                   cTATGGCCACCCGCAAAACCACCCCCCCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGTATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCCCCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTggg                                                                                               <  1:88309/150‑1 (MQ=255)
                                                      ttGCCACCCGCAAAACCACCACCGCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCg                                                                                            <  1:2308800/150‑1 (MQ=255)
                                                      ttGCCACCCGCAAAACCACAACCTCCCCCGCTGCTACCCGCAATAGCTGCGGCGTGAGTGTCGCGGATCGGTTGGTGGAATGTGGTGTT‑GGGCCACACCCCAACCCGCACCCCCACCCGGGGG‑TGGGGGGCCGGCGGGGCGTGTGGGTCg                                                                                            >  2:3724645/1‑150 (MQ=255)
                                                      ttGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCAAACCCCAACCCACAACCGCACCCGGGTG‑ATGGGGGCGGGCGTGGCCTGTtggggg                                                                                            >  1:7251733/1‑147 (MQ=255)
                                                        gCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCGAACCCCCACCCACTCACCCACGCGGGAG‑AGGGGGGCGGGCGGGCCGGTTGGGTCGAg                                                                                          >  2:1755236/1‑150 (MQ=255)
                                                         ccACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCAAAACCCCACGCGGGCG‑AGGGGGCCGGGCGTGGCGTGTggggggggg                                                                                         >  1:190825/1‑144 (MQ=255)
                                                             ccGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCct                                                                                     <  1:11158506/150‑1 (MQ=255)
                                                              cGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCACACCCCAAACCAAGACCCCCCGCCGGGG‑CGGGGGGCGGGCGGGCCGTGTGGGTGGAGGGccgc                                                                                    >  1:6478011/1‑148 (MQ=255)
                                                                cAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCctcgc                                                                                  <  2:5913978/150‑1 (MQ=255)
                                                                 aaaaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAGTGTGGTGTT‑GGGCCGCACCCCGACCCGGTCCCGCACGCGGGTG‑AGGGGGCCGGGGGGGGCATGTGGGGCGGGGGCcgcgcg                                                                                 >  2:7198793/1‑149 (MQ=255)
                                                                 aaaaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCctcgct                                                                                 <  1:7640212/150‑1 (MQ=255)
                                                                 aaaaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCGCAACCCAAACCACACCCGCACGCGGGTG‑TGGGGGCCGGGCGTGGCTTGGGGGGGGAGCGCctcgcg                                                                                 >  2:1620775/1‑149 (MQ=255)
                                                                 aaaaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCACACCCCAACCCACAACCCCACGCCGGAG‑AGGGGGCCGGGCGTGCCGTGGGGGTCGCGCGCcacgct                                                                                 >  2:5759273/1‑150 (MQ=255)
                                                                  aaaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCGCAACCCAAACCACTCCCGCCCGCGGGAG‑TGGGGGCCGGGCGTGCCTTGTGGGGAGAGCGCctcgcgc                                                                                >  2:6694060/1‑148 (MQ=255)
                                                                   aaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCctcgctcg                                                                               <  1:5179799/150‑1 (MQ=255)
                                                                   aaCCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCACACCCCAACCCACACCCCCACCCGGGAG‑CGGGGGCCGGGCGTGCCATGTGGGTCGAGCGCctcgcacg                                                                               >  1:8947635/1‑150 (MQ=255)
                                                                   aaCCACAACCTCCCCCGATGCTAACCGCAATAGCAGAGGCTTGAGTGTCTCGGATCGGTGTTTGGAATGTGGTGTT‑GGCCCACACCCCAACCCACACACACACGCGGGAG‑GTGGGGACGGGGGTGACAGGTGGGGCGAGCGCctcgctcg                                                                               >  2:8353704/1‑150 (MQ=255)
                                                                        caACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCACACCCGCCCGCGGGAG‑AGGGGGGCGGGCGGGGCGTGGGGGTGGAGCGCCACGCGCGGgcgc                                                                          >  2:2338935/1‑150 (MQ=255)
                                                                             tCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCt                                                                     <  1:259504/150‑1 (MQ=255)
                                                                             tCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTG‑GGGCCACAACCCAACCCAAACCCCCCCGCCGGGG‑TGGGGGGCGGGGGGGGCGTGTTGGGGGAGGGCCGCGCGCGGGGGCAGccg                                                                     >  1:655637/1‑149 (MQ=255)
                                                                             tCCCCCGCTGCTAACCGCAATAGCTGCGGCTGGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCCACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCt                                                                     <  2:1352458/150‑1 (MQ=255)
                                                                               ccccGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTgg                                                                   <  1:8094470/150‑1 (MQ=255)
                                                                               ccccGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCAAACCCCAACCCTCAACCCCACGCGGGGG‑GGGGGGGCGGGCGGGCCGTGTTGGGGGGGCGCCGCGCGCCGGCGCAGCCCgg                                                                   >  2:5108880/1‑150 (MQ=255)
                                                                                cccGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGa                                                                  <  1:7287441/150‑1 (MQ=255)
                                                                                 cctctgctAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGaa                                                                 <  2:10519401/147‑1 (MQ=255)
                                                                                   gctgctAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCACACCCCAAACCACAACCCCCCGCGGGCGTGGGGGGCGGGGGGTGCGGGGTGGG‑GGAGGGCCAAGCTCGGGCGCCGCCAGGAAcc                                                               >  2:2815608/1‑149 (MQ=255)
                                                                                     tgctAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑TGCCCACACCCCAAACCCAACCCCCACGCGGGGG‑ACGGGGGCCGGCGGGGCGGGTGGGGCGAGCGCCACCCGAGGGCGCAGCCAGGGACgct                                                             >  1:1163778/1‑148 (MQ=255)
                                                                                     tgctAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGAt                                                             <  2:7541078/150‑1 (MQ=255)
                                                                                     tgctAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCAACCCAACCCTCACCCCCACGCGGGAG‑AGGGGGGCGGGCGTGGCGTGTGGGTCGAGCGCCACGCTCGGGGGCAGCCGGGAACGAt                                                             >  2:1156857/1‑150 (MQ=255)
                                                                                       ctAACCGCAAGAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGt                                                           <  2:2726226/150‑1 (MQ=255)
                                                                                        tAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCAAACCCCAACCCGCACCCCCACGCGGGAG‑AGGGGGGCGGGCGTGCCGTGGGGGTCGAGCGCCGCCCACCGGCGCCGCCAGGTACGAttta                                                          >  2:6013260/1‑147 (MQ=255)
                                                                                          aCCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCACACCCCAACCCACACCCGCCAGCGGGAG‑GGGGGGCCGGGGGTGCCGGGTGGGTCGCGCGGCGCGCGCGGGGGCCGCCCGGCACCCTGTcgc                                                        >  2:6154543/1‑148 (MQ=255)
                                                                                           ccGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTAc                                                       <  2:9066590/150‑1 (MQ=255)
                                                                                              cAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCACAACCCCAACCACACCCCCCCCCCGGGGG‑GGGGGGCCGGG‑GGGGCGTGGGGGTGGAGCGCCGCGCTCGGGCGCAGCCAGGGACCATGtattacca                                                    >  1:5821061/1‑143 (MQ=255)
                                                                                                 tAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCACCCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTccc                                                 <  1:7046953/150‑1 (MQ=255)
                                                                                                  aGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTcccc                                                <  1:7559513/150‑1 (MQ=255)
                                                                                                  aGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTcccc                                                <  1:8001137/150‑1 (MQ=255)
                                                                                                   gCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCt                                               <  1:3212387/150‑1 (MQ=255)
                                                                                                   gCTGCGGCTTGAGTGTCGCGGATCGGTTTTGGGAATGGGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCt                                               <  2:5666546/150‑1 (MQ=255)
                                                                                                    cTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCAAAACCCAAACCACAGCCCCAACCCGGAG‑ATGGGGCCGGGCGTGGCGAGTTGGTCGCGGGCCACCCTCGGGCGCACCCAGGAACACTGTCGAACCAccccaa                                              >  2:4741812/1‑148 (MQ=255)
                                                                                                         gCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCCCACCCCCAACCACAACCCCCCCCCGGAG‑AGGGGGGCCGGCGGGGCGTGTGGGGGGCGCGCCACGCGCGGGGGCCGCCCGGGACCATTTGTAACCACCCCCAGGccc                                         >  1:6032628/1‑150 (MQ=255)
                                                                                                              agggtCGCGGATCGGATTTTGGACTGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGCGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCCAGGCCCCGGGt                                    <  2:5207891/147‑1 (MQ=255)
                                                                                                               gtgtCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCACACCCCAACCCACTACCGCCCGCGGGAG‑AGGGGGCCGGGCGTGGCATGTGGGTCGAGCGCCACGCACGGGCGCAGCCTGGAACGATGTCAACCCTCCCCTAGGCCCCgggga                                   >  2:7055397/1‑148 (MQ=255)
                                                                                                               gtgtCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCAAAACCCAAACCACTACCGCAACCGGGAG‑AGGGGGGCGGGAGTGGCGTTTGGGTGGATCGCCTCGCTCGGGCGCATACGGGCACGATGTGTAACCTCGCCTATGCGCCgggga                                   >  2:5854980/1‑148 (MQ=255)
                                                                                                                tgtCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTAt                                  <  2:10006981/150‑1 (MQ=255)
                                                                                                                tgtCGCGGATCGGTTTTTGGAATGGGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTAt                                  <  2:8097109/150‑1 (MQ=255)
                                                                                                                 gtCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGGCCGCACCCCAAACCAAACCCCCCCGCGGGCG‑AGGGGGGCGGGGGTGGCGAGTGGGTGGAGCGCCCCGCGGGGGGGCCGCCTGGTACCATGTGTAACCACCCCCAAGCCCCcgggtga                                 >  2:1926036/1‑146 (MQ=255)
                                                                                                                 gtCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAAACCAAACCCGCACCCGGGAG‑ACGGGGGCGGGCGTTCCGTGTGGGTCGAGCGCCGCGCTCGGGCGCCGCCTGGTACCATGTCTACCCTCCCCAAGGCCCCGGGTATc                                 >  2:10351739/1‑150 (MQ=255)
                                                                                                                  tCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCCCCCCTAGGCCCCGGGTATCt                                <  2:9631998/150‑1 (MQ=255)
                                                                                                                    gcgGATCGGTTTTTGGAATGTGGTGTT‑GGCCCTCAACCCAAACCACACCCGCACCCGGGAT‑AGGGGGCCGGGCGTGCCTTGTGGGTGGAGCGCCACGCGCGGGCGCAGCCTGGAACGATTTATACCCACCCCTAGGCCCCGGGTATCTgg                              >  2:4284081/1‑150 (MQ=255)
                                                                                                                        aTCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAAtt                          <  1:3309253/150‑1 (MQ=255)
                                                                                                                        aTCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAAtt                          <  2:305102/150‑1 (MQ=255)
                                                                                                                        aTCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAAtt                          <  1:8243194/150‑1 (MQ=255)
                                                                                                                         tCGGTTTTTGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTc                         <  1:2164778/150‑1 (MQ=255)
                                                                                                                                ttGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGccccc                  <  2:2566002/150‑1 (MQ=255)
                                                                                                                                ttGGAATGTGGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGccccc                  <  1:9319770/150‑1 (MQ=255)
                                                                                                                                      tgtgGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCTGGGTATCTGGAATTCGGCCcccagccca            <  2:6229223/150‑1 (MQ=255)
                                                                                                                                      tgtgGTGTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCcccagccca            <  1:2081979/150‑1 (MQ=255)
                                                                                                                                       gtggtgTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCcccagcccag           <  1:1376779/150‑1 (MQ=255)
                                                                                                                                        tggtgTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAgg          <  2:2396329/150‑1 (MQ=255)
                                                                                                                                        tggtgTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAgg          <  1:10061709/150‑1 (MQ=255)
                                                                                                                                         ggtgTT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGc         <  1:10710799/150‑1 (MQ=255)
                                                                                                                                            gtT‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGGCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCacc      >  2:457102/1‑148 (MQ=255)
                                                                                                                                             tt‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAAcc     <  1:6956336/150‑1 (MQ=255)
                                                                                                                                             tt‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAAcc     >  1:2882872/1‑150 (MQ=255)
                                                                                                                                             tt‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAAcc     <  2:9138331/150‑1 (MQ=255)
                                                                                                                                              t‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCa    <  2:10196462/150‑1 (MQ=255)
                                                                                                                                              t‑GGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCGTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCACCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCa    >  1:5630294/1‑150 (MQ=255)
                                                                                                                                                 gCCCTCACCCCCACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCaaa  <  2:4423981/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTT‑GGCCTTCACCCCAACCCTCTCCCGCACGCGGGAG‑AGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAA  >  NC_008463/3515716‑3516009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCTTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAAAGCA  >  NC_008463/3515714‑3516013
                                                                                                                                                     |                                                                                                                                                      
CGTATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCT                                                                                                                                                       <  SRR18135095.1358123/151‑1 (MQ=60)
CGTATACAGCCCCTCCCGAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCT                                                                                                                                                       <  SRR18135095.1556034/151‑1 (MQ=60)
 GTATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTC                                                                                                                                                      >  SRR18135095.9734339/1‑151 (MQ=60)
 GTATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTC                                                                                                                                                      <  SRR18135095.8360947/151‑1 (MQ=60)
    TACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACC                                                                                                                                                   <  SRR18135095.3699146/151‑1 (MQ=60)
      CAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCC                                                                                                                                                 <  SRR18135095.8209306/151‑1 (MQ=60)
          CCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCGCACCCCAACC                                                                                                                                             >  SRR18135095.5751902/1‑151 (MQ=60)
            CTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCT                                                                                                                                           <  SRR18135095.20358/151‑1 (MQ=60)
              CCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCT                                                                                                                                         <  SRR18135095.390855/151‑1 (MQ=60)
              CCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCT                                                                                                                                         <  SRR18135095.403983/151‑1 (MQ=60)
               CCTAAGCCATAGCCCTTCCCCATAACTGAACGGCACACCTATTGCCACCCGCAAAACCACATCCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTC                                                                                                                                        <  SRR18135095.5114822/151‑1 (MQ=60)
                     CCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCTCACCCCAAACCTCTCCCcccc                                                                                                                                  >  SRR18135095.9723753/1‑147 (MQ=60)
                     CCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCAC                                                                                                                                  <  SRR18135095.2150308/151‑1 (MQ=60)
                      CATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGTATGTGGTGTTTGGCCTCACCCCAACCCCCTCCCCCACG                                                                                                                                 >  SRR18135095.10947088/1‑151 (MQ=60)
                       ATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCACACCCCCACCCACTCCCGCACCC                                                                                                                                >  SRR18135095.10419896/1‑151 (MQ=60)
                         AGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCGAACCGCAATAGCTGCGGATTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGG                                                                                                                              <  SRR18135095.11120260/151‑1 (MQ=60)
                          GCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGG                                                                                                                             <  SRR18135095.7891360/151‑1 (MQ=60)
                           CCCTTCCCCATACCTGAACGGCACACCTCTTGCCACCCGCAAAACCACACCCGCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGA                                                                                                                            <  SRR18135095.3624960/151‑1 (MQ=60)
                            CCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAG                                                                                                                           <  SRR18135095.11030845/151‑1 (MQ=60)
                              TTCCCCATACCTGAACGGCACACCTCTTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAG                                                                                                                         <  SRR18135095.6710625/151‑1 (MQ=60)
                                CCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGG                                                                                                                       <  SRR18135095.1483555/151‑1 (MQ=60)
                                  CCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGG                                                                                                                     <  SRR18135095.10966425/151‑1 (MQ=60)
                                   CCTACCTGAACCGCACACCTATTGCCACCCTCAACACCACAACCTCCCCCGCTGATAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATAGGTTTTTTGAATGTGGTGTTGGCCCCCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGC                                                                                                                    <  SRR18135095.9583401/151‑1 (MQ=60)
                                   CATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGC                                                                                                                    <  SRR18135095.9583471/151‑1 (MQ=60)
                                    ATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCacaaccgcaccccggagtggggggc                                                                                                                   >  SRR18135095.6240369/1‑126 (MQ=60)
                                     TACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCGCACCCCAAACCACACCCGCACGCCGGGGAGGGGGGCG                                                                                                                  >  SRR18135095.8091708/1‑151 (MQ=60)
                                        CTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCACACCCCCACCCACTCCCGCACGCGGGggttgggggcgggg                                                                                                               >  SRR18135095.4894129/1‑137 (MQ=60)
                                         TGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCTCACCCCAACCCGCGCCCGCACCCGGGAGTGGGGGGCGGGCG                                                                                                              >  SRR18135095.4218723/1‑151 (MQ=60)
                                         TGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCGCACCCCAAACCACTCCCGCACGCGGGGGAGGGGGGCGGGGG                                                                                                              >  SRR18135095.6375601/1‑151 (MQ=60)
                                                    CCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGG                                                                                                   <  SRR18135095.4583758/151‑1 (MQ=60)
                                                     CTATGGCCACCCGCAAAACCACCCCCCCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGTATCGGTTTTTGGAATGTGGTGTTGGCCCTCCCCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGT                                                                                                  <  SRR18135095.88804/151‑1 (MQ=60)
                                                        TTGCCACCCGCAAAACCACCACCGCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGA                                                                                               <  SRR18135095.2319960/151‑1 (MQ=60)
                                                          GCCACCCGCAAAACCCCCACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGACC                                                                                             <  SRR18135095.524326/151‑1 (MQ=60)
                                                          GCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCAAAACCCCACGCGGGCGAGGGGGCCGGGCGTGgcgtgtggggggggg                                                                                             >  SRR18135095.191928/1‑136 (MQ=60)
                                                               CCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTC                                                                                        <  SRR18135095.2305686/151‑1 (MQ=60)
                                                               CCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTC                                                                                        <  SRR18135095.7428668/151‑1 (MQ=60)
                                                               CCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTC                                                                                        <  SRR18135095.11208022/151‑1 (MQ=60)
                                                                  CAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCACACCCCAACCCACACCCGCCCGCGGGTGAGGGGGGCGGGGGTGCCgtggggggcggggggcgcggg                                                                                     >  SRR18135095.9750097/1‑130 (MQ=60)
                                                                   AAAACCACACCCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTC                                                                                    <  SRR18135095.7675371/151‑1 (MQ=60)
                                                                   AAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTC                                                                                    <  SRR18135095.7675378/151‑1 (MQ=60)
                                                                    AAACCACCCCCACCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCACACCCCACCCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCG                                                                                   <  SRR18135095.6577975/151‑1 (MQ=60)
                                                                    AAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCACTCCCCCCCGCGGGAGAGGGGGCCGGGCGTGGCGTGTGGGTGGTGCGCCACGCTCG                                                                                   >  SRR18135095.8988131/1‑151 (MQ=60)
                                                                    AAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCACACCCCAACCCACACCCCCACCCGGGAGCGGGGGCCGGGCGTGCCATGTGGGTCGAGCGCCTCGCACG                                                                                   >  SRR18135095.8988093/1‑151 (MQ=60)
                                                                     AACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCACCCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGG                                                                                  <  SRR18135095.7051528/151‑1 (MQ=60)
                                                                     AACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGG                                                                                  <  SRR18135095.5204758/151‑1 (MQ=60)
                                                                       CCACACCCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGC                                                                                <  SRR18135095.3250787/151‑1 (MQ=60)
                                                                       CCACAACCTCCCCCGCTGCTAACCGCAATCGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGC                                                                                <  SRR18135095.3260625/151‑1 (MQ=60)
                                                                       CCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGC                                                                                <  SRR18135095.97168/151‑1 (MQ=60)
                                                                        CACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCTCACCCCAACCCAAACCCCCACCCCGGAGAGGGGGCCGGGCGTGCCGTGGGGGTCGCGCGCCacccaagggcg                                                                               >  SRR18135095.3187293/1‑140 (MQ=60)
                                                                             CCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCC                                                                          <  SRR18135095.7315868/151‑1 (MQ=60)
                                                                              CTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCCCACCCCCAACCAATCCCCCACCCGGGGGAGGGGGCCGGGCGTGGCTTGTGGGTCGAGCGCCTCGCTCGGGGGCAGCCA                                                                         >  SRR18135095.6942789/1‑151 (MQ=60)
                                                                               TCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTG                                                                        <  SRR18135095.260946/151‑1 (MQ=60)
                                                                               ACCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTG                                                                        <  SRR18135095.8154308/151‑1 (MQ=60)
                                                                                 CCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGA                                                                      <  SRR18135095.1280715/151‑1 (MQ=60)
                                                                                 CCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGA                                                                      <  SRR18135095.8131408/151‑1 (MQ=60)
                                                                                  CCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAA                                                                     <  SRR18135095.7321135/151‑1 (MQ=60)
                                                                                    CGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACG                                                                   <  SRR18135095.6914978/151‑1 (MQ=60)
                                                                                       TGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCTCACCCCCACCCGCTCCCCCCCGCGGGGGGGGGGGGCGGGCGTGgctgggggggggggcgccccgggggggggccgccggggacgctg                                                                >  SRR18135095.1682481/1‑107 (MQ=60)
                                                                                          TAACCGCAATAGCTGCGGCTTGTGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACACTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCT                                                             <  SRR18135095.11232789/151‑1 (MQ=60)
                                                                                            ACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCcacccaaacccccccgcgggggtcgggggcgcgcgggcggtgtggggggagcgcctcgcgcgggcgccgccgggaaagatgggtaa                                                           >  SRR18135095.3524092/1‑65 (MQ=60)
                                                                                              CGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCACCCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCC                                                         <  SRR18135095.5890133/151‑1 (MQ=60)
                                                                                                CAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGGCCACACCCCACCACCCACCCGCACGCCGGCGAGGGGGCCGGGCGTGgcgtggggggggggcgcctcgggcgggggccgcctggtccggtgtggacccac                                                       >  SRR18135095.2558496/1‑98 (MQ=60)
                                                                                                   TAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCACCCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCC                                                    <  SRR18135095.7079778/151‑1 (MQ=60)
                                                                                                   TAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCC                                                    <  SRR18135095.8556580/151‑1 (MQ=60)
                                                                                                    AGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCT                                                   <  SRR18135095.8037732/151‑1 (MQ=60)
                                                                                                    AGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCT                                                   <  SRR18135095.7621626/151‑1 (MQ=60)
                                                                                                    AGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCT                                                   <  SRR18135095.7594341/151‑1 (MQ=60)
                                                                                                     GCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTA                                                  <  SRR18135095.3227800/151‑1 (MQ=60)
                                                                                                      CTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAG                                                 <  SRR18135095.6162294/151‑1 (MQ=60)
                                                                                                       TGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTTGCCCGCACCCCAACCCcaacccccacgccggggaagggggcgggcggtgcatgtggggagaaggcccaccgcgggcggcgcccgggaacagttataaccacaccagag                                                >  SRR18135095.10949315/1‑59 (MQ=60)
                                                                                                                AGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGTGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTA                                       <  SRR18135095.11206307/151‑1 (MQ=60)
                                                                                                                AGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTA                                       <  SRR18135095.11218170/151‑1 (MQ=60)
                                                                                                                 GTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTAT                                      <  SRR18135095.9655141/151‑1 (MQ=60)
                                                                                                                   GTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCT                                    <  SRR18135095.1474158/151‑1 (MQ=60)
                                                                                                                      GCGGATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGA                                 <  SRR18135095.2350226/151‑1 (MQ=60)
                                                                                                                         GATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATT                              <  SRR18135095.3445057/151‑1 (MQ=60)
                                                                                                                          ATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTC                             <  SRR18135095.8280732/151‑1 (MQ=60)
                                                                                                                          ATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTC                             <  SRR18135095.7363698/151‑1 (MQ=60)
                                                                                                                          ATCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTC                             <  SRR18135095.3325116/151‑1 (MQ=60)
                                                                                                                           TCGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCG                            <  SRR18135095.2175268/151‑1 (MQ=60)
                                                                                                                            CGGTTTTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGG                           <  SRR18135095.315710/151‑1 (MQ=60)
                                                                                                                                 TTTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCC                      <  SRR18135095.7657537/151‑1 (MQ=60)
                                                                                                                                  TTGGAATGTGGTGTTGGCCCTCCCCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGACCGATGTCTACCCTCCCCTCGGCCCCGGGTATCTGGAATTCGGCCCCCA                     <  SRR18135095.4285914/151‑1 (MQ=60)
                                                                                                                                  TTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCA                     <  SRR18135095.3100926/151‑1 (MQ=60)
                                                                                                                                  TTGGAATGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCA                     <  SRR18135095.9361704/151‑1 (MQ=60)
                                                                                                                                        TGTGGTGTTGGCCCTCACCCCACCCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAG               <  SRR18135095.4188882/151‑1 (MQ=60)
                                                                                                                                        TGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAG               <  SRR18135095.2092069/151‑1 (MQ=60)
                                                                                                                                        TGTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAG               <  SRR18135095.592544/151‑1 (MQ=60)
                                                                                                                                         GTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGG              <  SRR18135095.1383549/151‑1 (MQ=60)
                                                                                                                                         GTGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGG              <  SRR18135095.2029425/151‑1 (MQ=60)
                                                                                                                                          TGGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGC             <  SRR18135095.10106756/151‑1 (MQ=60)
                                                                                                                                           GGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCA            <  SRR18135095.10758652/151‑1 (MQ=60)
                                                                                                                                           GGTGTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCA            <  SRR18135095.4605328/151‑1 (MQ=60)
                                                                                                                                              GTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACC         >  SRR18135095.2895850/1‑151 (MQ=60)
                                                                                                                                              GTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACC         >  SRR18135095.2896709/1‑151 (MQ=60)
                                                                                                                                              GTTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACC         <  SRR18135095.11130166/151‑1 (MQ=60)
                                                                                                                                               TTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCA        <  SRR18135095.6988801/151‑1 (MQ=60)
                                                                                                                                               TTGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCGTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCACCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCA        >  SRR18135095.5657342/1‑151 (MQ=60)
                                                                                                                                                TGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAA       >  SRR18135095.4404299/1‑151 (MQ=60)
                                                                                                                                                TGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAA       >  SRR18135095.8041613/1‑151 (MQ=60)
                                                                                                                                                TGGCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAA       <  SRR18135095.7044359/151‑1 (MQ=60)
                                                                                                                                                  GCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAAA     <  SRR18135095.9271811/151‑1 (MQ=60)
                                                                                                                                                  GCCCTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAAA     >  SRR18135095.10100616/1‑151 (MQ=60)
                                                                                                                                                     CTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAAAGCA  <  SRR18135095.3144383/151‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
CGTATACAGCCCCTCCCTAAGCCATAGCCCTTCCCCATACCTGAACGGCACACCTATTGCCACCCGCAAAACCACAACCTCCCCCGCTGCTAACCGCAATAGCTGCGGCTTGAGTGTCGCGGATCGGTTTTTGGAATGTGGTGTTGGCCTTCACCCCAACCCTCTCCCGCACGCGGGAGAGGGGGCCGGGCGTGCCTTGTGGGTCGAGCGCCTCGCTCGGGCGCAGCCTGGAACGATGTCTACCCTCCCCTAGGCCCCGGGTATCTGGAATTCGGCCCCCAGCCCAGGCAACCAAAAGCA  >  NC_008463/3515714‑3516013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑