Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	253	508.7	22197442	99.2%	22019862	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,787,871	Δ1 bp	pseudogene (949/3090 nt)	PA14_RS13085 →	CusA/CzcA family heavy metal efflux RND transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,787,871	0	A	.	99.1%	1596.5 / 8.3	333	pseudogene (949/3090 nt)	PA14_RS13085	CusA/CzcA family heavy metal efflux RND transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (155/172); minor base G (2/1); total (160/173)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 333 total aligned reads displayed.

CATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACC  >  NC_008463/2787741‑2788002

cATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaa                                                                                                                   >  2:3970921/1‑150 (MQ=255)

 aTGCTGATCGGCGAGAACAGACGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaag                                                                                                                  >  2:601003/1‑150 (MQ=255)

   gCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGGCCGGGGCCACG‑GTgaagaagaa                                                                                                                >  2:10657631/1‑150 (MQ=255)

   gCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaagaa                                                                                                                >  1:10325519/1‑150 (MQ=255)

    cTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAc                                                                                                               >  1:9883121/1‑150 (MQ=255)

     tGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcc                                                                                                              <  1:2477465/150‑1 (MQ=255)

      gATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGCCCC‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGGGAAGAAGAAcct                                                                                                             >  2:10704258/1‑149 (MQ=255)

      gATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcct                                                                                                             <  2:9305996/150‑2 (MQ=255)

       aTCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctg                                                                                                            <  2:5099801/150‑3 (MQ=255)

       aTCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctg                                                                                                            >  1:8332579/1‑148 (MQ=255)

       aTCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctg                                                                                                            >  1:2775946/1‑148 (MQ=255)

        tCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctgg                                                                                                           <  2:5260065/150‑4 (MQ=255)

        tCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctgg                                                                                                           <  1:4272889/150‑4 (MQ=255)

         cGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctggt                                                                                                          >  1:3813363/1‑146 (MQ=255)

          ggCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGACGAAGAA‑CCtggtg                                                                                                        >  2:6999921/1‑150 (MQ=255)

           gCGAGCACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCGGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCtggtgg                                                                                                       <  2:10253197/150‑1 (MQ=255)

           gCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCtggtgg                                                                                                       >  2:9064947/1‑150 (MQ=255)

           gCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCtggtgg                                                                                                       <  1:4821117/150‑1 (MQ=255)

             gagaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGaa                                                                                                     >  2:3477188/1‑150 (MQ=255)

             gagaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGaa                                                                                                     <  1:4181358/150‑1 (MQ=255)

             gagaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGaa                                                                                                     <  1:4945948/150‑1 (MQ=255)

              agaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAg                                                                                                    <  2:2683686/150‑1 (MQ=255)

              agaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAg                                                                                                    <  1:1956203/150‑1 (MQ=255)

               gaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAgg                                                                                                   <  1:8588916/150‑1 (MQ=255)

                aaCAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggc                                                                                                  >  1:7136115/1‑150 (MQ=255)

                aaCAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggc                                                                                                  >  1:7039838/1‑150 (MQ=255)

                aaCAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggc                                                                                                  <  2:8562398/150‑1 (MQ=255)

                 aCAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCAGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcg                                                                                                 >  1:1632541/1‑150 (MQ=255)

                  cAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgc                                                                                                <  2:8170025/150‑1 (MQ=255)

                    gCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcgg                                                                                              <  1:3513085/150‑1 (MQ=255)

                    gCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcgg                                                                                              <  1:8761186/150‑1 (MQ=255)

                    gCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcgg                                                                                              >  1:6067272/1‑150 (MQ=255)

                       gggAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTTGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcggcgc                                                                                           <  2:6725355/150‑1 (MQ=255)

                       gggAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcggcgc                                                                                           >  1:3214548/1‑150 (MQ=255)

                        ggAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCt                                                                                          <  1:2962831/150‑1 (MQ=255)

                         gAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTg                                                                                         >  1:5102304/1‑150 (MQ=255)

                             tGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGa                                                                                     <  2:1203154/150‑1 (MQ=255)

                             tGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGa                                                                                     <  1:9784845/150‑1 (MQ=255)

                               gCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATc                                                                                   >  2:6158471/1‑150 (MQ=255)

                                 ccAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATcgc                                                                                 <  1:5497261/150‑1 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCACCCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGAgg                                                                               <  2:4892286/148‑1 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                               >  2:3233522/3‑150 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                               <  1:5351945/148‑1 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                               >  1:10586916/3‑150 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGGGATCGCgg                                                                               >  1:10850318/3‑150 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCGGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                               >  2:8268643/3‑150 (MQ=255)

                                    ggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGt                                                                              >  1:2368868/2‑150 (MQ=255)

                                     gcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTg                                                                             <  1:9143285/150‑1 (MQ=255)

                                      cgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGACGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGc                                                                            <  2:6606439/150‑1 (MQ=255)

                                            gCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTcc                                                                      <  1:3046150/150‑1 (MQ=255)

                                             ccAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCt                                                                     <  1:9421723/150‑1 (MQ=255)

                                             ccAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCt                                                                     >  2:4392952/1‑150 (MQ=255)

                                             ccAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCt                                                                     <  1:788034/150‑1 (MQ=255)

                                             ccAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCt                                                                     <  2:10147005/150‑1 (MQ=255)

                                              cAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTg                                                                    <  2:6357566/150‑1 (MQ=255)

                                                gcgcCTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGtt                                                                  >  2:5441594/1‑150 (MQ=255)

                                                   ccTGGAGGAGATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCt                                                               >  1:9481085/1‑150 (MQ=255)

                                                       gaggagATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGc                                                           >  1:7241600/1‑150 (MQ=255)

                                                         ggagATCCACCGGACCCTGCCCC‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCaa                                                         <  2:10535464/150‑1 (MQ=255)

                                                         ggagATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCaa                                                         >  1:900938/1‑150 (MQ=255)

                                                         ggagATCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCaa                                                         <  2:4925140/150‑1 (MQ=255)

                                                           agaTCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACa                                                       >  2:2078775/1‑150 (MQ=255)

                                                             aTCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATc                                                     <  2:5281796/150‑1 (MQ=255)

                                                             aTCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATc                                                     <  2:962146/150‑1 (MQ=255)

                                                              tCAACCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATcc                                                    <  1:1834735/150‑1 (MQ=255)

                                                                aaCCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCcgc                                                  <  1:9525145/150‑1 (MQ=255)

                                                                 accgctcccTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCcgcg                                                 <  2:1556605/144‑1 (MQ=255)

                                                                 aCCGGACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCcgcg                                                 >  2:1796233/1‑150 (MQ=255)

                                                                    ggACCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCgcgg                                              <  2:1899816/150‑1 (MQ=255)

                                                                      aCCCTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCgcggcg                                            >  2:2465474/1‑150 (MQ=255)

                                                                      aCCCTG‑CCC‑ATGGGGGTCAAGGCGATCACCGTCTACGAACGCACCACCATGGTGGTCAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCgcggcg                                            >  2:7273108/1‑150 (MQ=255)

                                                                         cTG‑CCCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTg                                         >  2:4566802/1‑150 (MQ=255)

                                                                             cccaa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATc                                      <  1:6357046/146‑1 (MQ=255)

                                                                             cccaa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATc                                      <  1:6138033/146‑1 (MQ=255)

                                                                              ccaa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCa                                     <  2:2403416/147‑1 (MQ=255)

                                                                               caa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                    <  1:1499773/148‑1 (MQ=255)

                                                                               caa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                    <  2:2525748/148‑1 (MQ=255)

                                                                               caa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                    <  2:5819066/148‑1 (MQ=255)

                                                                               caa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                    >  1:9705964/3‑150 (MQ=255)

                                                                               caa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                    <  1:9076936/148‑1 (MQ=255)

                                                                                   gggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAGCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGc                                 >  2:654021/1‑150 (MQ=255)

                                                                                   gggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGc                                 <  1:2614765/150‑1 (MQ=255)

                                                                                   gggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGc                                 >  2:3722360/1‑150 (MQ=255)

                                                                                   gggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGc                                 <  1:3977331/150‑1 (MQ=255)

                                                                                      ggTCACGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGACGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGccac                              <  2:9698926/150‑1 (MQ=255)

                                                                                       gtcaaggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGccacc                             <  2:2249224/144‑1 (MQ=255)

                                                                                        tcaaggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGccacca                            <  1:8673614/145‑1 (MQ=255)

                                                                                          aaggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACcatc                          >  1:2189639/4‑150 (MQ=255)

                                                                                            ggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACcatcat                        <  2:9201151/149‑1 (MQ=255)

                                                                                               gATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATccc                     >  2:4127699/1‑150 (MQ=255)

                                                                                                  cACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCAGTTCCTCGGCACCATCCGCGCGGCGCTGAGCACCGCCACCATCATCCCGCt                  >  2:8082455/1‑150 (MQ=255)

                                                                                                      gTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCg              <  2:1440317/150‑1 (MQ=255)

                                                                                                       tCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCGTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGa             <  2:1097085/150‑1 (MQ=255)

                                                                                                        cTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCGGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGAt            <  2:2947484/150‑1 (MQ=255)

                                                                                                            gACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTg        <  1:4595973/150‑1 (MQ=255)

                                                                                                             aCCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGt       >  1:8396425/1‑150 (MQ=255)

                                                                                                              ccGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGtt      <  1:10493004/150‑1 (MQ=255)

                                                                                                               cGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGCTCGCGGTGCGCTGCGTGGTGCTCGGCTCCAGCCGCGCGGCGCTGATCAGCGCCACCATCATCCCGCTGTCGAGGCTGttc     >  2:3261409/1‑150 (MQ=255)

                                                                                                                  accaccCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGttcacc  <  1:10113389/150‑1 (MQ=255)

                                                                                                                  accaccCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGttcacc  >  2:2033350/1‑150 (MQ=255)

CATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTG‑CCC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACC  >  NC_008463/2787741‑2788002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCG  >  NC_008463/2787759‑2787990

CAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCG                                                                                   >  SRR18135100.6136097/1‑151 (MQ=60)

 AGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGG                                                                                  >  SRR18135100.6095967/1‑151 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                 <  SRR18135100.3529831/151‑1 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                 >  SRR18135100.8515576/1‑151 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                 >  SRR18135100.8515895/1‑151 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                 <  SRR18135100.8800528/151‑1 (MQ=60)

    CGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGC                                                                               >  SRR18135100.3229921/1‑151 (MQ=60)

    CGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGC                                                                               >  SRR18135100.4321652/1‑151 (MQ=60)

     GGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCT                                                                              <  SRR18135100.8919688/151‑1 (MQ=60)

     GGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCT                                                                              <  SRR18135100.4179578/151‑1 (MQ=60)

      GGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTG                                                                             <  SRR18135100.2977046/151‑1 (MQ=60)

      GGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTG                                                                             >  SRR18135100.5126793/1‑151 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                            >  SRR18135100.8990207/1‑151 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                            <  SRR18135100.2234300/151‑1 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                            <  SRR18135100.2189884/151‑1 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTG‑GTGGCCAAGGCCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                            >  SRR18135100.145295/1‑151 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                        <  SRR18135100.9828246/151‑1 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                        <  SRR18135100.1787854/151‑1 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                        >  SRR18135100.5078128/1‑151 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                        >  SRR18135100.912069/1‑151 (MQ=60)

            GGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATC                                                                       <  SRR18135100.10817091/151‑1 (MQ=60)

             GCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCG                                                                      >  SRR18135100.9529489/1‑151 (MQ=60)

               CCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCG                                                                    <  SRR18135100.5523526/151‑1 (MQ=60)

               CCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCG                                                                    >  SRR18135100.2728863/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGG                                                                   >  SRR18135100.10633579/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGG                                                                   >  SRR18135100.10633600/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGG                                                                   >  SRR18135100.8973389/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGGGATCGCGG                                                                   >  SRR18135100.10897992/1‑151 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                  <  SRR18135100.5377556/151‑1 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                  <  SRR18135100.9104402/151‑1 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                  >  SRR18135100.2380262/1‑151 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                  >  SRR18135100.4255577/1‑151 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                  <  SRR18135100.4040210/151‑1 (MQ=60)

                  GGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTG                                                                 >  SRR18135100.10159071/1‑151 (MQ=60)

                   GCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGC                                                                <  SRR18135100.8770918/151‑1 (MQ=60)

                   GCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGC                                                                <  SRR18135100.9184042/151‑1 (MQ=60)

                    CGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCT                                                               <  SRR18135100.3644921/151‑1 (MQ=60)

                     GGTCGGCCAGCGCCTGGAGGAGTTCAACCGGACCCTGCACA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCTCCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTC                                                              <  SRR18135100.255352/151‑1 (MQ=54)

                     GGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTC                                                              <  SRR18135100.6324738/151‑1 (MQ=60)

                     GGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGAGGCGCTGGTGTTCGCGGTGCTC                                                              >  SRR18135100.9214229/1‑151 (MQ=60)

                        CGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTC                                                           <  SRR18135100.1877859/151‑1 (MQ=60)

                          GCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCT                                                         >  SRR18135100.4290127/1‑151 (MQ=60)

                          GCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCT                                                         <  SRR18135100.3060549/151‑1 (MQ=60)

                          GCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCT                                                         <  SRR18135100.3060745/151‑1 (MQ=60)

                           CCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTG                                                        <  SRR18135100.9463673/151‑1 (MQ=60)

                           CCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTG                                                        <  SRR18135100.791888/151‑1 (MQ=60)

                               CGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCC                                                    >  SRR18135100.2724934/1‑151 (MQ=60)

                                GCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCT                                                   >  SRR18135100.9523280/1‑151 (MQ=60)

                                GCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCT                                                   <  SRR18135100.9925699/151‑1 (MQ=60)

                                GCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGCTCCT                                                   >  SRR18135100.10638006/1‑151 (MQ=60)

                                 CCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTC                                                  <  SRR18135100.1849473/151‑1 (MQ=60)

                                 CCTGGAGGAGATCAACCGGACCCTGC‑CC‑ATGGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTC                                                  >  SRR18135100.9938047/1‑151 (MQ=60)

                                  CTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCG                                                 >  SRR18135100.10480397/1‑151 (MQ=60)

                                  CTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCG                                                 <  SRR18135100.7844671/151‑1 (MQ=60)

                                   TGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGG                                                <  SRR18135100.8115445/151‑1 (MQ=60)

                                    GGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGC                                               >  SRR18135100.7274776/1‑151 (MQ=60)

                                    GGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAG‑ACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGGTCCTCGGC                                               <  SRR18135100.374592/151‑1 (MQ=60)

                                      AGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAA                                             >  SRR18135100.905291/1‑151 (MQ=60)

                                      AGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAA                                             >  SRR18135100.9534212/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGGGCTTCCTGTGCCTCGTCAAC                                            >  SRR18135100.5146289/1‑151 (MQ=54)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAC                                            >  SRR18135100.9470278/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAC                                            >  SRR18135100.9487606/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAC                                            >  SRR18135100.5462872/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGGTCCTCGGCAAC                                            <  SRR18135100.7250807/151‑1 (MQ=60)

                                        GAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACA                                           >  SRR18135100.1823512/1‑151 (MQ=60)

                                         AGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCacttcgaggtgcgcggccacag                                          >  SRR18135100.3763417/1‑129 (MQ=58)

                                          GATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATC                                         <  SRR18135100.4341373/151‑1 (MQ=60)

                                           ATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCC                                        <  SRR18135100.5608797/151‑1 (MQ=60)

                                           ATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCC                                        <  SRR18135100.49853/151‑1 (MQ=60)

                                            TCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCG                                       <  SRR18135100.3020095/151‑1 (MQ=60)

                                            TCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCG                                       <  SRR18135100.1843563/151‑1 (MQ=60)

                                             CAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGAGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGC                                      <  SRR18135100.872910/151‑1 (MQ=60)

                                              AACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCG                                     <  SRR18135100.9567514/151‑1 (MQ=60)

                                                 CGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGG                                  >  SRR18135100.8371906/1‑151 (MQ=60)

                                                   GACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCG                                <  SRR18135100.7153977/151‑1 (MQ=60)

                                                    ACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGC                               >  SRR18135100.1600266/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                             >  SRR18135100.2239593/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                             <  SRR18135100.10547243/151‑1 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                             >  SRR18135100.7198392/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                             >  SRR18135100.2125836/1‑151 (MQ=60)

                                                         GC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATC                          <  SRR18135100.2384556/151‑1 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                         <  SRR18135100.6386776/151‑1 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                         >  SRR18135100.478798/1‑151 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                         >  SRR18135100.3993066/1‑151 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                         <  SRR18135100.6166995/151‑1 (MQ=60)

                                                          C‑CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAC                        >  SRR18135100.9749064/1‑151 (MQ=60)

                                                          C‑CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAC                        <  SRR18135100.5236389/151‑1 (MQ=60)

                                                            CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACC                       <  SRR18135100.9117463/151‑1 (MQ=60)

                                                            CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACC                       <  SRR18135100.1507119/151‑1 (MQ=60)

                                                             A‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCG                      <  SRR18135100.555728/151‑1 (MQ=60)

                                                             A‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCG                      <  SRR18135100.4857702/151‑1 (MQ=60)

                                                               A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGC                     >  SRR18135100.5673130/1‑151 (MQ=60)

                                                               A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCGGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGC                     >  SRR18135100.1734160/1‑151 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                    <  SRR18135100.2627253/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                    <  SRR18135100.6330823/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                    <  SRR18135100.3983870/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                    <  SRR18135100.8696343/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                    <  SRR18135100.3996428/151‑1 (MQ=60)

                                                                   GGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCGTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCAC                  >  SRR18135100.3142194/1‑151 (MQ=60)

                                                                    GGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACC                 <  SRR18135100.8359916/151‑1 (MQ=60)

                                                                    GGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACC                 >  SRR18135100.8690969/1‑151 (MQ=60)

                                                                     GTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCA                >  SRR18135100.9906506/1‑151 (MQ=60)

                                                                      tcaagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCAT               <  SRR18135100.8712656/146‑1 (MQ=60)

                                                                       caagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATC              >  SRR18135100.10042500/5‑151 (MQ=60)

                                                                       caagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATC              >  SRR18135100.2200249/5‑151 (MQ=60)

                                                                        aagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCA             >  SRR18135100.107269/4‑151 (MQ=60)

                                                                         agGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCAT            >  SRR18135100.3249343/3‑151 (MQ=60)

                                                                          GGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATC           >  SRR18135100.3127759/1‑151 (MQ=60)

                                                                          GGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATC           <  SRR18135100.3948117/151‑1 (MQ=60)

                                                                           GCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCA          >  SRR18135100.978358/1‑151 (MQ=60)

                                                                            CGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCC         <  SRR18135100.9721541/151‑1 (MQ=60)

                                                                            CGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCC         <  SRR18135100.4775469/151‑1 (MQ=60)

                                                                             GATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCCTCATCCCG        >  SRR18135100.9143522/1‑151 (MQ=60)

                                                                             GATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCG        >  SRR18135100.7277507/1‑151 (MQ=60)

                                                                               TCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCT      <  SRR18135100.4343354/151‑1 (MQ=60)

                                                                                 ACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGT    <  SRR18135100.540796/151‑1 (MQ=60)

                                                                                  CCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTC   <  SRR18135100.2232249/151‑1 (MQ=60)

                                                                                  CCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTC   <  SRR18135100.6124030/151‑1 (MQ=60)

                                                                                   CGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCG  >  SRR18135100.1041513/1‑151 (MQ=60)

CAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCG  >  NC_008463/2787759‑2787990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`