Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	253	508.7	22197442	99.2%	22019862	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	2,787,877	C→G	pseudogene (955/3090 nt)	PA14_RS13085 →	CusA/CzcA family heavy metal efflux RND transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	2,787,877	0	C	G	99.2%	1361.6 / 0.8	356	pseudogene (955/3090 nt)	PA14_RS13085	CusA/CzcA family heavy metal efflux RND transporter
Reads supporting (aligned to +/- strand): ref base C (3/0); new base G (172/181); total (175/181)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 356 total aligned reads displayed.

CATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGCAAC  >  NC_008463/2787741‑2788023

cATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaa                                                                                                                                        <  2:8525241/150‑1 (MQ=255)

cATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaa                                                                                                                                        >  2:10319402/1‑150 (MQ=255)

 aTGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaag                                                                                                                                       <  2:10945596/150‑1 (MQ=255)

 aTGCTGATCGGCGAGAACAGACGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaag                                                                                                                                       >  2:601003/1‑150 (MQ=255)

  tGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAGCCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCGACGACCGCTCCACCCTGGTGGACAAGGCCGGGGCCACG‑GTgaagaaga                                                                                                                                      >  2:2132659/1‑150 (MQ=255)

   gCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaagaa                                                                                                                                     >  1:10325519/1‑150 (MQ=255)

   gCTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTgaagaagaa                                                                                                                                     <  1:152991/150‑1 (MQ=255)

    cTGATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAc                                                                                                                                    >  1:9119434/1‑150 (MQ=255)

      gATCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcct                                                                                                                                  <  2:9305996/150‑2 (MQ=255)

       aTCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctg                                                                                                                                 <  2:5099801/150‑3 (MQ=255)

       aTCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctg                                                                                                                                 >  1:8332579/1‑148 (MQ=255)

       aTCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctg                                                                                                                                 >  1:9343629/1‑148 (MQ=255)

        tCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctgg                                                                                                                                <  2:5260065/150‑4 (MQ=255)

        tCGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctgg                                                                                                                                <  1:4272889/150‑4 (MQ=255)

         cGGCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAAcctggt                                                                                                                               >  1:3813363/1‑146 (MQ=255)

          ggCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGACGAAGAA‑CCtggtg                                                                                                                             >  2:6999921/1‑150 (MQ=255)

          ggCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCtggtg                                                                                                                             <  2:10993004/150‑1 (MQ=255)

           gCGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCtggtgg                                                                                                                            >  2:9064947/1‑150 (MQ=255)

            cGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTCCGACCGCACCACCCTG‑GTGGCCAAGGCCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGa                                                                                                                           >  2:10187211/1‑150 (MQ=255)

            cGAGAACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCCCCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGa                                                                                                                           >  1:3369627/1‑150 (MQ=255)

             gagaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGaa                                                                                                                          <  1:4181358/150‑1 (MQ=255)

             gagaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGaa                                                                                                                          <  1:4945948/150‑1 (MQ=255)

             gagaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGaa                                                                                                                          >  2:3477188/1‑150 (MQ=255)

               gaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAgg                                                                                                                        >  1:1109695/1‑150 (MQ=255)

               gaACAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAgg                                                                                                                        <  1:2382875/150‑1 (MQ=255)

                 aCAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcg                                                                                                                      <  1:2827026/150‑1 (MQ=255)

                   aGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcg                                                                                                                    >  2:9488001/1‑150 (MQ=255)

                    gCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcgg                                                                                                                   >  1:6067272/1‑150 (MQ=255)

                    gCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcgg                                                                                                                   >  2:2050948/1‑150 (MQ=255)

                     ccGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcggc                                                                                                                  >  1:8477621/1‑150 (MQ=255)

                      cGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAggcgcggcg                                                                                                                 >  2:5812261/1‑150 (MQ=255)

                         gAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTg                                                                                                              >  1:5102304/1‑150 (MQ=255)

                         gAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTg                                                                                                              <  1:2223537/150‑1 (MQ=255)

                            gTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCCCCCTGGTGGTCAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTg                                                                                                           <  2:1830150/150‑1 (MQ=255)

                             tGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGa                                                                                                          <  1:1779259/150‑1 (MQ=255)

                             tGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGa                                                                                                          <  1:9784845/150‑1 (MQ=255)

                             tGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGa                                                                                                          <  2:1203154/150‑1 (MQ=255)

                                cccAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATcg                                                                                                       <  2:2276194/150‑1 (MQ=255)

                                  caggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATcgcg                                                                                                     >  1:2715860/4‑150 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCACCCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGAgg                                                                                                    <  2:4892286/148‑1 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                                                    >  1:8933373/3‑150 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                                                    <  1:9063922/148‑1 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                                                    <  1:5351945/148‑1 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                                                    >  2:3233522/3‑150 (MQ=255)

                                   aggcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCGGGTGGAAGGCGCGGCGCTGGTGATCGCgg                                                                                                    >  2:8268643/3‑150 (MQ=255)

                                    cgcgGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTTCGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCAGGTGGAAGGCGCGGCGCTGGTGATCGCGGt                                                                                                   <  2:6163813/150‑1 (MQ=255)

                                       ggTCGGCCAGCGCCTGGAGGAGTTCAACCGGACCCTGCACA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCTCCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGct                                                                                                <  1:253969/150‑1 (MQ=255)

                                         tcggCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGctct                                                                                              <  2:2908391/147‑1 (MQ=255)

                                            gCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTcc                                                                                           <  1:3045955/150‑1 (MQ=255)

                                            gCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTcc                                                                                           <  2:1981180/150‑1 (MQ=255)

                                             ccAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCt                                                                                          <  1:788034/150‑1 (MQ=255)

                                              cAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTg                                                                                         <  2:6357566/150‑1 (MQ=255)

                                                gcgcCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGtt                                                                                       <  2:502724/150‑1 (MQ=255)

                                                   ccTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCt                                                                                    <  1:1840614/150‑1 (MQ=255)

                                                   ccTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGCTCCt                                                                                    >  1:10591305/1‑150 (MQ=255)

                                                    cTGGAGGAGATCAACCGGACCCTGC‑CC‑ATGGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTc                                                                                   >  1:9894261/1‑150 (MQ=255)

                                                       gaggagATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGc                                                                                >  1:7241600/1‑150 (MQ=255)

                                                          gagaTCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAc                                                                             >  1:9445551/1‑150 (MQ=255)

                                                             aTCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATc                                                                          <  2:5281796/150‑1 (MQ=255)

                                                               cAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGAGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCcg                                                                        <  1:868703/150‑1 (MQ=255)

                                                                aaCCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCcgc                                                                       <  1:9525145/150‑1 (MQ=255)

                                                                     gACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCgcggc                                                                  <  1:7121222/150‑1 (MQ=255)

                                                                       cccTGC‑CC‑ACGGGGGTCAAGGCGCTCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGACGAAG‑ACCCTGGCGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACCTCCGCGCGgcgc                                                                <  2:1722304/150‑1 (MQ=255)

                                                                        ccTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCt                                                               <  1:10500920/150‑1 (MQ=255)

                                                                              ccaa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCa                                                          >  2:6725928/4‑150 (MQ=255)

                                                                              ccaa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCa                                                          >  1:476356/4‑150 (MQ=255)

                                                                              ccaa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCa                                                          >  1:3973981/4‑150 (MQ=255)

                                                                               caa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                                         >  2:8109289/3‑150 (MQ=255)

                                                                                cacgggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAc                                                         <  2:2519701/147‑1 (MQ=255)

                                                                                aa‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAcc                                                        <  1:552942/149‑1 (MQ=255)

                                                                                   gggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGc                                                      <  1:6301276/150‑1 (MQ=255)

                                                                                   gggggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGc                                                      >  2:3722360/1‑150 (MQ=255)

                                                                                      ggTCACGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGACGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGccac                                                   <  2:9698926/150‑1 (MQ=255)

                                                                                      ggTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCGTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGccac                                                   >  1:3127224/1‑150 (MQ=255)

                                                                                       gtcaaggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGccacc                                                  >  1:8652018/7‑150 (MQ=255)

                                                                                         caaggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACcat                                                <  2:10661065/146‑1 (MQ=255)

                                                                                         caaggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACcat                                                <  2:6643154/146‑1 (MQ=255)

                                                                                            ggCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACcatcat                                             <  2:9201151/149‑1 (MQ=255)

                                                                                              cGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATcc                                           <  1:9678558/150‑1 (MQ=255)

                                                                                               gATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATccc                                          >  2:4127699/1‑150 (MQ=255)

                                                                                                    ccGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGt                                     <  2:4665613/150‑1 (MQ=255)

                                                                                                    ccGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCGGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCGTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGt                                     <  2:7519471/150‑1 (MQ=255)

                                                                                                    ccGTCTACGACCGCACCACCCTGGTGGAC‑ACGGCCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGt                                     <  2:3995951/150‑1 (MQ=255)

                                                                                                      gTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCg                                   <  2:1440317/150‑1 (MQ=255)

                                                                                                        cTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGAt                                 <  2:6556814/150‑1 (MQ=255)

                                                                                                         tACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATg                                >  2:2638050/1‑150 (MQ=255)

                                                                                                           cGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCt                              <  1:656946/150‑1 (MQ=255)

                                                                                                              ccGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGtt                           >  2:10587108/1‑150 (MQ=255)

                                                                                                               cGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGCTCGCGGTGCGCTGCGTGGTGCTCGGCTCCAGCCGCGCGGCGCTGATCAGCGCCACCATCATCCCGCTGTCGAGGCTGttc                          >  2:3261409/1‑150 (MQ=255)

                                                                                                                gCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGttca                         >  2:2454534/1‑150 (MQ=255)

                                                                                                                  accaccCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGttcacc                       <  1:10113389/150‑1 (MQ=255)

                                                                                                                                 caaggccgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGt         <  1:1996922/143‑1 (MQ=255)

                                                                                                                                   aggccgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCg       >  2:5453226/6‑150 (MQ=255)

                                                                                                                                    ggccgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCgg      >  1:6164169/5‑150 (MQ=255)

                                                                                                                                    ggccgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCgg      >  1:4512830/5‑150 (MQ=255)

                                                                                                                                     gccgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGc     >  2:72814/4‑150 (MQ=255)

                                                                                                                                      ccgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGCa    >  1:8474947/3‑150 (MQ=255)

                                                                                                                                       cgTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCACTACCAGGTAATCGGCTACAACCGCGCGTCGCGGCTCAACGCAACAAAAAACCACGTGTCTAGGCTGTAAACGTTAACAGGGATGGTCGGCaa   >  1:9738223/2‑150 (MQ=255)

                                                                                                                                        gTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGCAAc  >  2:4492939/1‑150 (MQ=255)

                                                                                                                                        gTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCCGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTACACCTTCACCGGGATGGTCGGCAAc  <  1:2185618/150‑1 (MQ=255)

CATGCTGATCGGCGAGAACAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGCAAC  >  NC_008463/2787741‑2788023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGCAACA  >  NC_008463/2787759‑2788024

CAGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCG                                                                                                                     >  SRR18135100.6136097/1‑151 (MQ=60)

 AGCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGG                                                                                                                    >  SRR18135100.6095967/1‑151 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                                                   <  SRR18135100.3529831/151‑1 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                                                   >  SRR18135100.8515576/1‑151 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                                                   >  SRR18135100.8515895/1‑151 (MQ=60)

  GCCGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGC                                                                                                                   <  SRR18135100.8800528/151‑1 (MQ=60)

    CGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGC                                                                                                                 >  SRR18135100.3229921/1‑151 (MQ=60)

    CGGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGC                                                                                                                 >  SRR18135100.4321652/1‑151 (MQ=60)

     GGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCT                                                                                                                <  SRR18135100.4179578/151‑1 (MQ=60)

     GGGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCT                                                                                                                <  SRR18135100.8919688/151‑1 (MQ=60)

      GGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTG                                                                                                               <  SRR18135100.2977046/151‑1 (MQ=60)

      GGAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTG                                                                                                               >  SRR18135100.5126793/1‑151 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                                                              >  SRR18135100.8990207/1‑151 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                                                              <  SRR18135100.2189884/151‑1 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                                                              <  SRR18135100.2234300/151‑1 (MQ=60)

       GAAGTGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTG‑GTGGCCAAGGCCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGG                                                                                                              >  SRR18135100.145295/1‑151 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                                                          <  SRR18135100.1787854/151‑1 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                                                          >  SRR18135100.5078128/1‑151 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                                                          <  SRR18135100.9828246/151‑1 (MQ=60)

           TGGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGAT                                                                                                          >  SRR18135100.912069/1‑151 (MQ=60)

            GGCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATC                                                                                                         <  SRR18135100.10817091/151‑1 (MQ=60)

             GCCCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCG                                                                                                        >  SRR18135100.9529489/1‑151 (MQ=60)

               CCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCG                                                                                                      <  SRR18135100.5523526/151‑1 (MQ=60)

               CCAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCG                                                                                                      >  SRR18135100.2728863/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGG                                                                                                     >  SRR18135100.8973389/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGG                                                                                                     >  SRR18135100.10633600/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGG                                                                                                     >  SRR18135100.10633579/1‑151 (MQ=60)

                CAGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGGGATCGCGG                                                                                                     >  SRR18135100.10897992/1‑151 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                                                    >  SRR18135100.2380262/1‑151 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                                                    <  SRR18135100.4040210/151‑1 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                                                    >  SRR18135100.4255577/1‑151 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                                                    <  SRR18135100.9104402/151‑1 (MQ=60)

                 AGGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGT                                                                                                    <  SRR18135100.5377556/151‑1 (MQ=60)

                  GGCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTG                                                                                                   >  SRR18135100.10159071/1‑151 (MQ=60)

                   GCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGC                                                                                                  <  SRR18135100.8770918/151‑1 (MQ=60)

                   GCGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGC                                                                                                  <  SRR18135100.9184042/151‑1 (MQ=60)

                    CGGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCT                                                                                                 <  SRR18135100.3644921/151‑1 (MQ=60)

                     GGTCGGCCAGCGCCTGGAGGAGTTCAACCGGACCCTGCACA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCTCCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTC                                                                                                <  SRR18135100.255352/151‑1 (MQ=54)

                     GGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTC                                                                                                <  SRR18135100.6324738/151‑1 (MQ=60)

                     GGTCGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGAGGCGCTGGTGTTCGCGGTGCTC                                                                                                >  SRR18135100.9214229/1‑151 (MQ=60)

                        CGGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTC                                                                                             <  SRR18135100.1877859/151‑1 (MQ=60)

                          GCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCT                                                                                           >  SRR18135100.4290127/1‑151 (MQ=60)

                          GCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCT                                                                                           <  SRR18135100.3060745/151‑1 (MQ=60)

                          GCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCT                                                                                           <  SRR18135100.3060549/151‑1 (MQ=60)

                           CCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTG                                                                                          <  SRR18135100.9463673/151‑1 (MQ=60)

                           CCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTG                                                                                          <  SRR18135100.791888/151‑1 (MQ=60)

                               CGCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCC                                                                                      >  SRR18135100.2724934/1‑151 (MQ=60)

                                GCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCT                                                                                     >  SRR18135100.9523280/1‑151 (MQ=60)

                                GCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCT                                                                                     <  SRR18135100.9925699/151‑1 (MQ=60)

                                GCCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGCTCCT                                                                                     >  SRR18135100.10638006/1‑151 (MQ=60)

                                 CCTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTC                                                                                    <  SRR18135100.1849473/151‑1 (MQ=60)

                                 CCTGGAGGAGATCAACCGGACCCTGC‑CC‑ATGGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTC                                                                                    >  SRR18135100.9938047/1‑151 (MQ=60)

                                  CTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCG                                                                                   <  SRR18135100.7844671/151‑1 (MQ=60)

                                  CTGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCG                                                                                   >  SRR18135100.10480397/1‑151 (MQ=60)

                                   TGGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGG                                                                                  <  SRR18135100.8115445/151‑1 (MQ=60)

                                    GGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGC                                                                                 >  SRR18135100.7274776/1‑151 (MQ=60)

                                    GGAGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAG‑ACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGGTCCTCGGC                                                                                 <  SRR18135100.374592/151‑1 (MQ=60)

                                      AGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAA                                                                               >  SRR18135100.905291/1‑151 (MQ=60)

                                      AGGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAA                                                                               >  SRR18135100.9534212/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGGGCTTCCTGTGCCTCGTCAAC                                                                              >  SRR18135100.5146289/1‑151 (MQ=54)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAC                                                                              >  SRR18135100.9487606/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAC                                                                              >  SRR18135100.5462872/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAAC                                                                              >  SRR18135100.9470278/1‑151 (MQ=60)

                                       GGAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGGTCCTCGGCAAC                                                                              <  SRR18135100.7250807/151‑1 (MQ=60)

                                        GAGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACA                                                                             >  SRR18135100.1823512/1‑151 (MQ=60)

                                         AGATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCacttcgaggtgcgcggccacag                                                                            >  SRR18135100.3763417/1‑129 (MQ=58)

                                          GATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATC                                                                           <  SRR18135100.4341373/151‑1 (MQ=60)

                                           ATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACCAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCC                                                                          <  SRR18135100.5608797/151‑1 (MQ=60)

                                           ATCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCC                                                                          <  SRR18135100.49853/151‑1 (MQ=60)

                                            TCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCG                                                                         <  SRR18135100.3020095/151‑1 (MQ=60)

                                            TCAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCG                                                                         <  SRR18135100.1843563/151‑1 (MQ=60)

                                             CAACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGAGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGC                                                                        <  SRR18135100.872910/151‑1 (MQ=60)

                                              AACCGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCG                                                                       <  SRR18135100.9567514/151‑1 (MQ=60)

                                                 CGGACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGG                                                                    >  SRR18135100.8371906/1‑151 (MQ=60)

                                                   GACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCG                                                                  <  SRR18135100.7153977/151‑1 (MQ=60)

                                                    ACCCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGC                                                                 >  SRR18135100.1600266/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                                                               >  SRR18135100.2125836/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                                                               >  SRR18135100.2239593/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                                                               >  SRR18135100.7198392/1‑151 (MQ=60)

                                                      CCTGC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTG                                                               <  SRR18135100.10547243/151‑1 (MQ=60)

                                                         GC‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATC                                                            <  SRR18135100.2384556/151‑1 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                                                           <  SRR18135100.6386776/151‑1 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                                                           >  SRR18135100.3993066/1‑151 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                                                           <  SRR18135100.6166995/151‑1 (MQ=60)

                                                          C‑CCAA‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCA                                                           >  SRR18135100.478798/1‑151 (MQ=60)

                                                          C‑CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAC                                                          <  SRR18135100.5236389/151‑1 (MQ=60)

                                                          C‑CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCAC                                                          >  SRR18135100.9749064/1‑151 (MQ=60)

                                                            CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACC                                                         <  SRR18135100.1507119/151‑1 (MQ=60)

                                                            CA‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACC                                                         <  SRR18135100.9117463/151‑1 (MQ=60)

                                                             A‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCG                                                        <  SRR18135100.4857702/151‑1 (MQ=60)

                                                             A‑A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCG                                                        <  SRR18135100.555728/151‑1 (MQ=60)

                                                               A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGC                                                       >  SRR18135100.5673130/1‑151 (MQ=60)

                                                               A‑GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCGGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGC                                                       >  SRR18135100.1734160/1‑151 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                                                      <  SRR18135100.8696343/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                                                      <  SRR18135100.3996428/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                                                      <  SRR18135100.3983870/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                                                      <  SRR18135100.2627253/151‑1 (MQ=60)

                                                                 GGGGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCC                                                      <  SRR18135100.6330823/151‑1 (MQ=60)

                                                                   GGGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCGTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCAC                                                    >  SRR18135100.3142194/1‑151 (MQ=60)

                                                                    GGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACC                                                   <  SRR18135100.8359916/151‑1 (MQ=60)

                                                                    GGTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACC                                                   >  SRR18135100.8690969/1‑151 (MQ=60)

                                                                     GTCAAGGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCA                                                  >  SRR18135100.9906506/1‑151 (MQ=60)

                                                                      tcaagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCAT                                                 <  SRR18135100.8712656/146‑1 (MQ=60)

                                                                       caagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATC                                                >  SRR18135100.10042500/5‑151 (MQ=60)

                                                                       caagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATC                                                >  SRR18135100.2200249/5‑151 (MQ=60)

                                                                        aagGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCA                                               >  SRR18135100.107269/4‑151 (MQ=60)

                                                                         agGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCAT                                              >  SRR18135100.3249343/3‑151 (MQ=60)

                                                                          GGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATC                                             <  SRR18135100.3948117/151‑1 (MQ=60)

                                                                          GGCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATC                                             >  SRR18135100.3127759/1‑151 (MQ=60)

                                                                           GCGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCA                                            >  SRR18135100.978358/1‑151 (MQ=60)

                                                                            CGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCC                                           <  SRR18135100.4775469/151‑1 (MQ=60)

                                                                            CGATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCC                                           <  SRR18135100.9721541/151‑1 (MQ=60)

                                                                             GATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCCTCATCCCG                                          >  SRR18135100.9143522/1‑151 (MQ=60)

                                                                             GATCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCG                                          >  SRR18135100.7277507/1‑151 (MQ=60)

                                                                               TCACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCT                                        <  SRR18135100.4343354/151‑1 (MQ=60)

                                                                                 ACCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGT                                      <  SRR18135100.540796/151‑1 (MQ=60)

                                                                                  CCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTC                                     <  SRR18135100.2232249/151‑1 (MQ=60)

                                                                                  CCGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTC                                     <  SRR18135100.6124030/151‑1 (MQ=60)

                                                                                   CGTCTACGACCGCACCACCCTGGTGGACAAGG‑CCGTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCG                                    >  SRR18135100.1041513/1‑151 (MQ=60)

                                                                                                                      GTGGCCACGGTGAAGAAGAA‑CCTGGTGGAAGGCGCGGCGCCGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTACACCTTCACCGGGATGGTCGGCAACA  <  SRR18135100.2196210/151‑1 (MQ=60)

CAGCCGGGAAGTGGCCCACGCGGTCTGCCAGCGCCTGGAGGAGATCAACCGGACCCTGC‑CC‑A‑GGGGGTCACTGCGATCACCGTCTACGACCGCACCACCCTGCATGACTAGGACAGTGCCCAC‑GTGAAGAAGAACCCTGGTGGAAGGCGCGGCGCTGGTGATCGCGGTGCTCTTCCTGTTCCTCGGCAACATCCGCGCGGCGCTGATCACCGCCACCATCATCCCGCTGTCGATGCTGTTCACCTTCACCGGGATGGTCGGCAACA  >  NC_008463/2787759‑2788024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`