Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 256 507.2 22121016 99.2% 21944047 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,162 N→C intergenic (+23/‑38) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1620NC100.0% 1673.7 / NA 450intergenic (+23/‑38)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  new base C (231/219);  total (231/219)

Only 100 of 454 total aligned reads displayed.

GGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128014‑1128310
                                                                                                                                                    |                                                                                                                                                    
 gggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCgg                                                                                                                                                    <  2:1273847/150‑1 (MQ=255)
   gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                  <  2:6241360/150‑1 (MQ=255)
   gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                  <  1:2961102/150‑1 (MQ=255)
   gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                  >  1:5966686/1‑150 (MQ=255)
    tGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGcc                                                                                                                                                 >  2:9184363/1‑150 (MQ=255)
      gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCgg                                                                                                                                               <  2:1865419/150‑1 (MQ=255)
      gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTAGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGTACCGGGCCgg                                                                                                                                               >  1:7692831/1‑150 (MQ=255)
       aCGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGc                                                                                                                                              >  2:5786657/1‑150 (MQ=255)
          cgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAg                                                                                                                                           <  1:9826514/150‑1 (MQ=255)
              gTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAgg                                                                                                                                       >  2:8914075/1‑150 (MQ=255)
               tACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGa                                                                                                                                      <  2:2262759/150‑1 (MQ=255)
               tACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGa                                                                                                                                      >  1:9740116/1‑150 (MQ=255)
                aCCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAc                                                                                                                                     >  2:5801112/1‑150 (MQ=255)
                aCCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAc                                                                                                                                     >  1:7249490/1‑150 (MQ=255)
                 ccAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACt                                                                                                                                    <  1:4254684/150‑1 (MQ=255)
                   aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTa                                                                                                                                  >  1:4552367/1‑150 (MQ=255)
                   aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTa                                                                                                                                  <  1:5420153/150‑1 (MQ=255)
                    gAACACCTACACCGCGAGCCTCTCCTACACCCACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGACTTaa                                                                                                                                 >  1:2481013/1‑150 (MQ=255)
                    gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTaa                                                                                                                                 >  1:10840566/1‑150 (MQ=255)
                    gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTaa                                                                                                                                 >  1:7175878/1‑150 (MQ=255)
                    gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACATAGTCGCGCACAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTat                                                                                                                                 >  2:1471560/1‑149 (MQ=255)
                     aaCACCTACACCGCTAGCCTCTCCTACACCAACTACTTCGACGGCAAGTACACCACCGTGGACGAACGCGACTTCTTCGCGCAAAGCTGCGGCATTAAATTATGATGTACCCCGGCCCCGTGCGAAACGGGCCGGCGATCAAGACTTaaa                                                                                                                                >  1:811880/1‑149 (MQ=255)
                     aaCACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                                <  2:278191/150‑1 (MQ=255)
                     aaCACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAc                                                                                                                                <  2:3811701/150‑1 (MQ=255)
                         ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAgg                                                                                                                            >  2:10086949/1‑150 (MQ=255)
                         ccTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCGCAGCTTAGGCATGAAATTCAGATGTTAGCCGGCCCCGTGGGAACCGGGCCGGCGAGCAGGTCATAACGAgg                                                                                                                            >  1:5111042/1‑150 (MQ=255)
                          cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGACTTAACGAGga                                                                                                                           >  1:8762386/1‑150 (MQ=255)
                          cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                           <  2:2383344/150‑1 (MQ=255)
                           tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                          <  1:1002647/150‑1 (MQ=255)
                           tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                          <  1:3624348/150‑1 (MQ=255)
                            acacCGCGAGCCTCTCCTACACCAACGTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTAGGCCTGCACTTCTGATGTACGCCGGCCCCGTGCGGACCGGGCCGGGGAGCAGGGCTTAACGAGggcg                                                                                                                         >  1:9344291/1‑150 (MQ=255)
                               ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGacgacg                                                                                                                      <  1:3149414/150‑1 (MQ=255)
                                 gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                                    <  1:9599496/150‑1 (MQ=255)
                                 gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                                    <  1:676333/150‑1 (MQ=255)
                                 gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCGTCGGCATGAACGTCTGAAGTACGCCGGCCCGGTGCGCCCCGGGCCGGCGCGCAGGACTTAACGAGGACGACGAg                                                                                                                    >  2:969360/1‑150 (MQ=255)
                                   gAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                                  <  2:2621859/150‑1 (MQ=255)
                                     gCCTCTCCTACCCCCACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGaa                                                                                                                <  2:4336605/150‑1 (MQ=255)
                                      ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                               <  2:771678/150‑1 (MQ=255)
                                       ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                              <  1:6954719/150‑1 (MQ=255)
                                        tctcCTACACCAACTTCTTCGACGGCAAGTACACCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGa                                                                                                             <  1:1037833/150‑1 (MQ=255)
                                        tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGa                                                                                                             >  2:2936810/1‑150 (MQ=255)
                                         cgccTACACCAACTTCGTCGACCGCAAGGACACCACCGTGGACGACCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGTCGAGCAGGACTTAACGAGGACGACGAGCGAATGaa                                                                                                            <  2:6120501/148‑1 (MQ=255)
                                            cTACACCAACTTCTTCGACGGCAAGTACACCCCCGTGGACGCACGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                         <  1:10576101/150‑1 (MQ=255)
                                            cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                         >  2:727083/1‑150 (MQ=255)
                                            cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaac                                                                                                         <  1:6432872/150‑1 (MQ=255)
                                            cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCGCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGTGCAGGACTTAACGAGGACGACGGGCGAATGAAaac                                                                                                         >  2:5614506/1‑150 (MQ=255)
                                             tACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAaaca                                                                                                        <  1:5268332/150‑1 (MQ=255)
                                                acCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGATGACGACGAGCGAATGTAAACAACg                                                                                                     >  1:582177/1‑150 (MQ=255)
                                                acCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTATGATGTACGCCCGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAAGAGGACGACGAGCGAATGAAAACAACg                                                                                                     >  1:8652008/1‑150 (MQ=255)
                                                  cAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGaa                                                                                                   <  1:10683286/150‑1 (MQ=255)
                                                   aaCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAAGAGGACGACGAGCGAATGAAAACAACGAAg                                                                                                  >  1:1377346/1‑150 (MQ=255)
                                                     cttcttCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAt                                                                                                <  2:4774262/150‑1 (MQ=255)
                                                     cttcttCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAt                                                                                                <  2:10667461/150‑1 (MQ=255)
                                                      ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAtt                                                                                               <  2:5220332/150‑1 (MQ=255)
                                                        cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttct                                                                                             <  1:4043737/150‑1 (MQ=255)
                                                           cGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCGTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGCACCGGGCCGGCGAGCAGGACTTAACGTGGACGACGAGCGAATGAAAACAACGAAGATtcttct                                                                                          >  2:3173133/1‑150 (MQ=255)
                                                             aCGGCAAGTACACCACCGTGGACTATCGCGACTTCGTCGCGCTCAGCTTCGGAATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGc                                                                                        <  1:4676202/150‑1 (MQ=255)
                                                               ggCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGTCGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                      >  2:3039840/1‑150 (MQ=255)
                                                                     tACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGgt                                                                                <  2:8107789/150‑1 (MQ=255)
                                                                      acacCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGGACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGTCGAGCGAATGAAAACAACGTAGATTCTTCTGCATACCGgtg                                                                               >  2:10214342/1‑150 (MQ=255)
                                                                             cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGc                                                                        <  2:1692879/150‑1 (MQ=255)
                                                                               tGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGccc                                                                      <  1:2673178/150‑1 (MQ=255)
                                                                                ggACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCt                                                                     <  2:7403767/150‑1 (MQ=255)
                                                                                 gACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTg                                                                    <  1:1522832/150‑1 (MQ=255)
                                                                                  aCGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGa                                                                   >  2:8253441/1‑150 (MQ=255)
                                                                                    gATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGTTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGc                                                                 <  2:2520700/150‑1 (MQ=255)
                                                                                    gATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGc                                                                 >  2:1762183/1‑150 (MQ=255)
                                                                                     aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCCTACCGGTGTACTGGCCCTGAGcc                                                                >  1:5605039/1‑150 (MQ=255)
                                                                                      tCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTTATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGAAGAGCGAATGAATACAACGAAGATTCTTCTGCATACCGGTGTAATGGCCCTGAGCct                                                               <  2:8592131/150‑1 (MQ=255)
                                                                                         cgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgct                                                            >  1:4198443/1‑150 (MQ=255)
                                                                                          gACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGAGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGCATGAAAACACCGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgctg                                                           <  1:3452644/150‑1 (MQ=255)
                                                                                            cTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGc                                                         <  1:3847041/150‑1 (MQ=255)
                                                                                               cgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTGAACGCGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAc                                                      <  2:3214331/150‑1 (MQ=255)
                                                                                                gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                                     >  1:1656846/1‑150 (MQ=255)
                                                                                                gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                                     >  1:2356041/1‑150 (MQ=255)
                                                                                                gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                                     >  2:8348362/1‑150 (MQ=255)
                                                                                                 tcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAccc                                                    >  1:6118882/1‑150 (MQ=255)
                                                                                                   gcgcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAg                                                  <  2:2090953/150‑1 (MQ=255)
                                                                                                   gcgcTCAGCTTCGGCATGAACTTAAGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAACACAACGAAGAGTCTACTGCATACCGGTGGGCTGGCCCTGAGCCTGCGGGCCACCCAg                                                  >  2:4557707/1‑150 (MQ=255)
                                                                                                       tCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGa                                              <  2:785877/150‑1 (MQ=255)
                                                                                                              cGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcggc                                       <  1:2854659/150‑1 (MQ=255)
                                                                                                                gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGcggcgg                                     <  1:1950391/150‑1 (MQ=255)
                                                                                                                    gaccTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATc                                 >  2:6579385/4‑150 (MQ=255)
                                                                                                                    gAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATc                                 >  2:3558507/1‑150 (MQ=255)
                                                                                                                     aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATcc                                >  1:1807467/1‑150 (MQ=255)
                                                                                                                       cTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgc                              >  1:4737261/1‑150 (MQ=255)
                                                                                                                        ttCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgcc                             >  1:8004512/1‑150 (MQ=255)
                                                                                                                           tGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATTGCGGCGGTATCCGCcgac                          >  1:1141228/1‑150 (MQ=255)
                                                                                                                             aTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacga                        >  2:10839442/1‑150 (MQ=255)
                                                                                                                              tGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAg                       <  1:6216748/150‑1 (MQ=255)
                                                                                                                               gTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAgg                      >  1:3267667/1‑150 (MQ=255)
                                                                                                                               gTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGGATCCGCCGACGAgg                      >  2:10372581/1‑150 (MQ=255)
                                                                                                                                     cGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCa                >  1:8896583/1‑150 (MQ=255)
                                                                                                                                      ggCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGAATCCGCCGACGAGTCGGCCac               >  2:10446767/1‑149 (MQ=255)
                                                                                                                                       gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg              >  1:5534840/1‑150 (MQ=255)
                                                                                                                                             ggcgaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGc        <  1:122374/146‑1 (MQ=255)
                                                                                                                                               cgaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCAc      >  2:6412136/3‑150 (MQ=255)
                                                                                                                                                 aaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCa    >  1:7035896/1‑150 (MQ=255)
                                                                                                                                                   ccGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGc  >  2:6995293/1‑150 (MQ=255)
                                                                                                                                                   ccGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGc  <  1:7295604/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128014‑1128310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A