Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 256 507.2 22121016 99.2% 21944047 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,028,197 Δ1 bp intergenic (‑119/+18) PA14_RS22975 ← / ← PA14_RS22980 Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,028,1950G.99.4% 2289.3 / 10.2 473intergenic (‑117/+20)PA14_RS22975/PA14_RS22980Nramp family divalent metal transporter/ribbon‑helix‑helix domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base . (241/225);  total (245/228)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 499 total aligned reads displayed.

TGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCC  >  NC_008463/5028045‑5028338
                                                                                                                                                      |                                                                                                                                               
tGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCg                                                                                                                                                 <  1:3677959/150‑1 (MQ=255)
   gcAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtc                                                                                                                                              <  1:7555804/150‑3 (MQ=255)
    cAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcg                                                                                                                                             <  2:8613417/150‑4 (MQ=255)
     aGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgc                                                                                                                                            <  1:47586/150‑5 (MQ=255)
     aGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgc                                                                                                                                            <  1:234669/150‑5 (MQ=255)
       cggcgAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCggtcgcgt                                                                                                                                          >  1:3480614/1‑144 (MQ=255)
                    gccggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTccctcccctc                                                                                                                            <  2:1572610/150‑1 (MQ=255)
                       ggcACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAgg                                                                                                                         >  1:1933967/1‑150 (MQ=255)
                         cACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGcc                                                                                                                       >  1:7902767/1‑150 (MQ=255)
                          aCCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCt                                                                                                                      <  2:4599282/150‑1 (MQ=255)
                           ccGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTg                                                                                                                     >  2:6875223/1‑150 (MQ=255)
                             gCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                                                                                                   <  1:9089738/150‑1 (MQ=255)
                             gCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                                                                                                   <  1:5970509/150‑1 (MQ=255)
                             gCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                                                                                                   <  2:1118184/150‑1 (MQ=255)
                             gCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTggg                                                                                                                   >  1:2055270/1‑150 (MQ=255)
                                   cTGTGGCTGACCGCCGTGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCTGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCt                                                                                                             <  1:4297607/150‑1 (MQ=255)
                                     gtgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTGCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGGCTTCCCTCCCCTCCGGCCGGGGCGCGCTgg                                                                                                           >  1:9799341/1‑150 (MQ=255)
                                      tgGCTGACCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGc                                                                                                          <  2:5705239/150‑1 (MQ=255)
                                            aCCGCCGAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCGCCCCTCAGGCCTGGGCGCGCTGGCCGACCa                                                                                                    <  2:7468439/150‑1 (MQ=255)
                                               gccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGCGGAAGTGCGACCAGCCTAAACGTTGCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCGGGGCGCGCTGGCCGACCGGGc                                                                                                 >  1:1513054/1‑150 (MQ=255)
                                               gccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGc                                                                                                 >  2:6854047/1‑150 (MQ=255)
                                               gccgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGc                                                                                                 <  1:617404/150‑1 (MQ=255)
                                                 cgAGGGACAGATCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTc                                                                                               <  2:7171958/150‑1 (MQ=255)
                                                 cgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTc                                                                                               <  2:5444524/150‑1 (MQ=255)
                                                 cgAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTc                                                                                               >  2:3454828/1‑150 (MQ=255)
                                                  gAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCa                                                                                              <  1:10726756/150‑1 (MQ=255)
                                                  gAGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCAAAACGATTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCa                                                                                              >  1:7816592/1‑150 (MQ=255)
                                                   aGGGACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGACGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAg                                                                                             >  2:5928945/1‑150 (MQ=255)
                                                     ggACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCt                                                                                           <  1:4570518/150‑1 (MQ=255)
                                                     ggACAGAACGGCGCGGAGG‑CCGTGGTGGGCAGGGAGTAGTTCACGCGGCGACGCCGGATCGGGGAGGAAGAGCGACCAGCCTAAACGTTGCACCCCGGG‑CGCCTCTTCCCTCCCCTCCCGCCAGGGCGCGCTGGCCGACCAGGCTCAGCt                                                                                           <  2:10461927/150‑1 (MQ=255)
                                                      gACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                                                                                          >  1:7738840/1‑150 (MQ=255)
                                                      gACAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTc                                                                                          >  2:8447174/1‑150 (MQ=255)
                                                       aCAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCg                                                                                         >  2:8858231/1‑150 (MQ=255)
                                                       aCAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCg                                                                                         <  2:7926600/150‑1 (MQ=255)
                                                        cAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCgg                                                                                        >  2:9527656/1‑150 (MQ=255)
                                                        cAGAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCgg                                                                                        <  2:7107784/150‑1 (MQ=255)
                                                          gAACGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCg                                                                                      >  1:8638864/1‑150 (MQ=255)
                                                           aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGa                                                                                     >  2:2771120/1‑150 (MQ=255)
                                                           aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGa                                                                                     >  2:448241/1‑150 (MQ=255)
                                                           aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGa                                                                                     >  1:1102096/1‑150 (MQ=255)
                                                           aaCGGCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCNTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGa                                                                                     <  1:5785771/150‑1 (MQ=255)
                                                              ggCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCt                                                                                  <  2:1241406/150‑1 (MQ=255)
                                                              ggCGCGGTGG‑CCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCt                                                                                  <  2:480027/150‑1 (MQ=255)
                                                                     gtggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgc                                                                            >  1:5461406/5‑150 (MQ=255)
                                                                      tggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTcgcg                                                                           >  2:10252357/4‑150 (MQ=255)
                                                                       ggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCgcgc                                                                          <  1:10143035/148‑1 (MQ=255)
                                                                       ggccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCgcgc                                                                          >  1:1707075/3‑150 (MQ=255)
                                                                        gccGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgcc                                                                         <  2:8081922/149‑1 (MQ=255)
                                                                          cGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgccgc                                                                       <  1:8948761/150‑1 (MQ=255)
                                                                          cGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGcgccgc                                                                       <  1:3085901/150‑1 (MQ=255)
                                                                            tggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTg                                                                     <  1:6800046/150‑1 (MQ=255)
                                                                            tggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTg                                                                     >  2:2428942/1‑150 (MQ=255)
                                                                            tggtggGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTg                                                                     <  1:3910676/150‑1 (MQ=255)
                                                                                gggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGcc                                                                 <  2:2248856/150‑1 (MQ=255)
                                                                                 ggCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCa                                                                <  1:2588419/150‑1 (MQ=255)
                                                                                    aGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGt                                                             >  1:10350012/1‑150 (MQ=255)
                                                                                      ggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAg                                                           >  2:8322687/1‑150 (MQ=255)
                                                                                      ggAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAg                                                           >  1:8540841/1‑150 (MQ=255)
                                                                                       gAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGc                                                          <  1:7429377/150‑1 (MQ=255)
                                                                                       gAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGc                                                          <  1:2877553/150‑1 (MQ=255)
                                                                                         gTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTNGCgg                                                        <  1:10893317/150‑1 (MQ=255)
                                                                                         gTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCgg                                                        <  1:5394144/150‑1 (MQ=255)
                                                                                          tATTTCCCGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCggg                                                       <  2:10923823/150‑1 (MQ=255)
                                                                                              tCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCa                                                   >  1:5066525/1‑150 (MQ=255)
                                                                                              tCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCa                                                   >  2:7294172/1‑150 (MQ=255)
                                                                                                  gAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTg                                               <  2:10104437/150‑1 (MQ=255)
                                                                                                  gAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTg                                               >  2:6657666/1‑150 (MQ=255)
                                                                                                   aGGCGACGCCGGATCGGGGCGGAAGGGCGACCAGCCGAACCGTGGCACCCC‑GGTCGCGTCTTCCCTCCCCACAGGCCGGGGCGCGCTGCCCGACCAGGCTCAGCCCGGCGACCTTCTCGCGCCGCTGCCCCAGGGAGCGGGTGCAACTGa                                              <  2:4321518/150‑1 (MQ=255)
                                                                                                   aGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGa                                              >  1:1004214/1‑150 (MQ=255)
                                                                                                    ggCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGac                                             <  2:9771685/150‑1 (MQ=255)
                                                                                                    ggCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGac                                             >  2:3710325/1‑150 (MQ=255)
                                                                                                     gCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGaca                                            <  2:2834584/150‑1 (MQ=255)
                                                                                                        aCGCCGGATCGGGGAGGAAGTGCGACCAGCCTATACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGTCCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGGAGCGGGTGCAACTGACACGc                                         >  2:10779297/1‑150 (MQ=255)
                                                                                                        aCGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGc                                         <  1:5262943/150‑1 (MQ=255)
                                                                                                          gCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCGCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCGGCGCCAGGTAGCGGGTGCAACTGACACGCag                                       >  1:1062017/1‑150 (MQ=255)
                                                                                                           ccGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTGCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCagg                                      <  1:4080066/150‑1 (MQ=255)
                                                                                                              gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                   <  2:4097107/150‑1 (MQ=255)
                                                                                                              gATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaag                                   <  1:10655025/150‑1 (MQ=255)
                                                                                                               aTCGGGGAGGTTTTGCGACCAGCTTCAACGTTTCACCCC‑GGTCGGTTCTTCCCTCCCCTCAGGCCTGGGAGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCATCTGAAACGCaggaagg                                  <  2:2715904/150‑1 (MQ=255)
                                                                                                               aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagg                                  <  1:9537093/150‑1 (MQ=255)
                                                                                                               aTCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCaggaagg                                  <  1:4185375/150‑1 (MQ=255)
                                                                                                                  ggggAGGAAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAaggagg                               <  1:8876868/150‑1 (MQ=255)
                                                                                                                       ggaAGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAg                          >  2:2056275/1‑150 (MQ=255)
                                                                                                                        gcaGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGt                         <  1:3262973/148‑1 (MQ=255)
                                                                                                                          aGTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCGTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTg                       <  2:9306837/150‑1 (MQ=255)
                                                                                                                           gTGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGa                      <  2:5846790/150‑1 (MQ=255)
                                                                                                                            tGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAg                     >  2:6291090/1‑150 (MQ=255)
                                                                                                                            tGCGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAg                     <  1:6544664/150‑1 (MQ=255)
                                                                                                                              cGACCAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCa                   >  1:8131996/1‑150 (MQ=255)
                                                                                                                              cGACCAGCCGAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGTGCa                   <  2:3089004/150‑1 (MQ=255)
                                                                                                                                 ccAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                >  1:2729242/1‑150 (MQ=255)
                                                                                                                                 ccAGCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCt                <  2:5251452/150‑1 (MQ=255)
                                                                                                                                    gCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc             <  2:6415073/150‑1 (MQ=255)
                                                                                                                                    gCCTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgc             <  2:8201524/150‑1 (MQ=255)
                                                                                                                                     ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc            <  1:7010182/150‑1 (MQ=255)
                                                                                                                                     ccTAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCGCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgcc            >  1:843302/1‑150 (MQ=255)
                                                                                                                                       tAAACGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTcgccgc          <  2:7766194/150‑1 (MQ=255)
                                                                                                                                          aCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt       >  2:5028782/1‑150 (MQ=255)
                                                                                                                                          aCGTTTCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTGCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACGGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGAt       >  2:9081650/1‑150 (MQ=255)
                                                                                                                                              ttCACCCC‑GGTCGCGTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTAAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTc   >  1:5736983/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
TGCGCAGCGGCGAGGCGTCGGCCGGCACCGCCACGCTGTGGCTGACCGCCGAGGGACAGAACGGCGCGGTGGCCCGTGGTGGGCAGGGAGTATTTCACGAGGCGACGCCGGATCGGGGAGGAAGTGCGACCAGCCTAAACGTTTCACCCCGGGTGGGTTCTTCCCTCCCCTCAGGCCTGGGCGCGCTGGCCGACCAGGCTCAGCTCGGCGACCTTCTCGCGCCGCTGCGCCAGGTAGCGGGTGCAACTGACACGCAGGAAGGAGGCGAAGTTGAGCACCTCGCCGCGATAGTCC  >  NC_008463/5028045‑5028338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A