Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	256	492.8	21425088	99.3%	21275112	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	4,947,649	2 bp→GG	coding (463‑464/1416 nt)	PA14_RS22670 →	DNA polymerase Y family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	4,947,649	0	A	G	99.6%	1075.2 / NA	274	N155G (AAC→GGC)	PA14_RS22670	DNA polymerase Y family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (150/123); minor base T (1/0); total (151/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	4,947,650	0	A	G	100.0%	1074.7 / NA	274	N155G (AAC→GGC)	PA14_RS22670	DNA polymerase Y family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (151/123); total (151/123)

Only 100 of 274 total aligned reads displayed.

CCTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGT‑CAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGC  >  NC_008463/4947522‑4947760

||

ccTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGAc                                                                                           <  2:139673/150‑1 (MQ=255)

 cTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCCTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                          >  1:8634859/1‑150 (MQ=255)

 cTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACa                                                                                          <  2:5724546/150‑1 (MQ=255)

   gTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgc                                                                                        <  1:4964366/150‑1 (MQ=255)

   gTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgc                                                                                        >  1:1411592/1‑150 (MQ=255)

   gTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCGGGCCATCGAGGACGAGACAgc                                                                                        <  1:6942062/150‑1 (MQ=255)

    ttCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAgcg                                                                                       >  2:5390311/1‑150 (MQ=255)

       ggCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTg                                                                                    >  2:4441910/1‑150 (MQ=255)

        gCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGc                                                                                   >  1:7395614/1‑150 (MQ=255)

         ccccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCa                                                                                  >  1:1608810/1‑150 (MQ=255)

           ccTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAac                                                                                <  2:2032896/150‑1 (MQ=255)

           ccTGGCCACGCTGCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAac                                                                                <  1:3948956/150‑1 (MQ=255)

            cTGGCCACGCGTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAacg                                                                               >  2:5598967/1‑149 (MQ=255)

              ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                             >  1:3213304/1‑150 (MQ=255)

              ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                             <  1:6278051/150‑1 (MQ=255)

              ggCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGcc                                                                             >  2:9694001/1‑150 (MQ=255)

               gCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCa                                                                            >  2:8577196/1‑150 (MQ=255)

                  aCGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGtt                                                                         <  1:5751804/150‑1 (MQ=255)

                    gCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGt                                                                       <  2:8844811/150‑1 (MQ=255)

                     cTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGACTGGCGCAGAACCCTGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCTCTGCAACGCCAGTTGTc                                                                      >  2:8067794/1‑150 (MQ=255)

                      ttCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                     >  2:7757496/1‑150 (MQ=255)

                      ttCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                     <  1:8712392/150‑1 (MQ=255)

                      ttCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTcc                                                                     >  2:4149164/1‑150 (MQ=255)

                       tCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCg                                                                    >  2:4282061/1‑150 (MQ=255)

                        cGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGCAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGc                                                                   >  2:6500964/1‑150 (MQ=255)

                         gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                  <  2:1290246/150‑1 (MQ=255)

                         gAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGcc                                                                  >  1:10554772/1‑150 (MQ=255)

                          aGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGccc                                                                 >  2:8771044/1‑150 (MQ=255)

                            gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                               >  1:6599944/1‑150 (MQ=255)

                            gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                               >  1:1228041/1‑150 (MQ=255)

                            gCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCtt                                                               >  2:399987/1‑150 (MQ=255)

                             cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                              >  1:1970187/1‑150 (MQ=255)

                             cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                              >  2:4543435/1‑150 (MQ=255)

                             cccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTc                                                              <  2:6997471/150‑1 (MQ=255)

                              ccGCTTGCGCACGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTcc                                                             <  1:846083/150‑1 (MQ=255)

                              ccGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTcc                                                             <  1:4615237/150‑1 (MQ=255)

                                  tggcgcAAGGAACTCACGGCGCTGGGCGTCCGCCCTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTg                                                         <  1:5988323/148‑1 (MQ=255)

                                    gcgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGa                                                       <  1:2215866/150‑1 (MQ=255)

                                     cgcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAc                                                      >  1:5137707/1‑150 (MQ=255)

                                      gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                     <  2:1707840/150‑1 (MQ=255)

                                      gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                     <  1:8367395/150‑1 (MQ=255)

                                      gcAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCT‑GGCGACCAGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAcc                                                     <  2:18481/150‑1 (MQ=255)

                                         aGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGcc                                                  >  2:2690660/1‑150 (MQ=255)

                                          ggAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTACCCAGGACCGCCt                                                 >  2:5986233/1‑150 (MQ=255)

                                           gAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTg                                                >  2:2307511/1‑150 (MQ=255)

                                            aaCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGAAGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTgg                                               <  1:4482081/150‑1 (MQ=255)

                                             aCTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTggg                                              >  1:4221514/1‑150 (MQ=255)

                                              cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                             >  1:3733450/1‑150 (MQ=255)

                                              cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                             >  2:3928932/1‑150 (MQ=255)

                                              cTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGa                                             >  2:7244780/1‑150 (MQ=255)

                                               tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                            >  2:5237555/1‑150 (MQ=255)

                                               tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                            >  1:4337879/1‑150 (MQ=255)

                                               tCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAc                                            >  2:1278154/1‑150 (MQ=255)

                                                cACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGCct                                           >  2:10412496/1‑150 (MQ=255)

                                                cACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAct                                           <  1:1734240/150‑1 (MQ=255)

                                                 aCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActc                                          <  2:10201437/150‑1 (MQ=255)

                                                 aCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActc                                          <  1:4754001/150‑1 (MQ=255)

                                                 aCGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActc                                          >  1:2813765/1‑150 (MQ=255)

                                                  cGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGActct                                         <  1:5319526/150‑1 (MQ=255)

                                                   ggCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACtctc                                        >  2:5929035/1‑150 (MQ=255)

                                                     cgcTGGGCGTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTcgc                                      <  1:9865926/150‑1 (MQ=255)

                                                       cTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCgg                                    >  1:10652710/1‑150 (MQ=255)

                                                        tGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCCGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCggg                                   >  2:6689319/1‑150 (MQ=255)

                                                         gggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                  <  2:4666438/150‑1 (MQ=255)

                                                         gggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                  >  1:4429569/1‑150 (MQ=255)

                                                         gggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                  <  1:1214721/150‑1 (MQ=255)

                                                         gggCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGa                                  >  2:9821295/1‑150 (MQ=255)

                                                           gCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGc                                >  2:354527/1‑150 (MQ=255)

                                                            cTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCt                               <  2:8367766/150‑1 (MQ=255)

                                                             ttCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTg                              <  1:3003328/150‑1 (MQ=255)

                                                             ttCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTg                              <  2:1074598/150‑1 (MQ=255)

                                                               ccGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGc                            >  1:681135/1‑150 (MQ=255)

                                                                 gCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCg                          >  1:969520/1‑150 (MQ=255)

                                                                  ccATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGt                         >  2:7340382/1‑150 (MQ=255)

                                                                    aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGTGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                       >  2:350591/1‑150 (MQ=255)

                                                                    aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                       >  1:10391236/1‑150 (MQ=255)

                                                                    aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                       >  1:185620/1‑150 (MQ=255)

                                                                    aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                       >  2:9461510/1‑150 (MQ=255)

                                                                    aTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCg                       >  1:8197468/1‑150 (MQ=255)

                                                                      cGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGcc                     >  2:7111998/1‑150 (MQ=255)

                                                                        ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                   <  2:5287645/150‑1 (MQ=255)

                                                                        ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                   >  1:4684708/1‑150 (MQ=255)

                                                                        ccTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCt                   <  1:409589/150‑1 (MQ=255)

                                                                         cTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTc                  >  1:9740675/1‑150 (MQ=255)

                                                                             gCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc              <  2:9051565/150‑1 (MQ=255)

                                                                             gCCTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGAc              >  2:9605953/1‑150 (MQ=255)

                                                                              ccTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACg             <  2:2882908/150‑1 (MQ=255)

                                                                              ccTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACg             <  1:9007438/150‑1 (MQ=255)

                                                                               cTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGc            <  1:9987866/150‑1 (MQ=255)

                                                                               cTGGCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGc            >  2:1143988/1‑150 (MQ=255)

                                                                                tGGCGCCGAACCCGGTGGCGGCGAGGGTCCTT‑GCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCa           >  2:755083/1‑150 (MQ=255)

                                                                                 ggCGCCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCAt          <  2:3045872/150‑1 (MQ=255)

                                                                                   cgcCGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATgg        <  1:9352280/150‑1 (MQ=255)

                                                                                      cGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGAc     <  2:3134151/150‑1 (MQ=255)

                                                                                      cGAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGAc     >  1:7673998/1‑150 (MQ=255)

                                                                                       gAACCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACt    <  1:2615382/150‑1 (MQ=255)

                                                                                         aCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc  >  1:9807381/1‑150 (MQ=255)

                                                                                         aCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc  <  2:2217667/150‑1 (MQ=255)

                                                                                         aCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc  >  2:3807662/1‑150 (MQ=255)

                                                                                         aCCCGGTGGCGGCGAGGGT‑CCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTgc  <  1:9517694/150‑1 (MQ=255)

||

CCTGTTCGGCCCCTGGCCACGCTTCGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGT‑CAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCGCCCTCGGACGCATGGGACTGC  >  NC_008463/4947522‑4947760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  NC_008463/4947546‑4947739

CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                             >  SRR18135075.10596782/1‑151 (MQ=60)

CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                             >  SRR18135075.3156169/1‑151 (MQ=60)

CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCC                                             >  SRR18135075.903654/1‑151 (MQ=60)

 GAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCC                                            >  SRR18135075.1467035/1‑151 (MQ=60)

  AGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCT                                           >  SRR18135075.7716457/1‑151 (MQ=60)

   GGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTT                                          >  SRR18135075.289156/1‑151 (MQ=60)

   GGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTT                                          >  SRR18135075.289138/1‑151 (MQ=60)

   GGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTT                                          >  SRR18135075.1233722/1‑151 (MQ=60)

   GGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTT                                          >  SRR18135075.6627613/1‑151 (MQ=60)

    GCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTC                                         >  SRR18135075.4248554/1‑151 (MQ=60)

    GCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTC                                         >  SRR18135075.1978948/1‑151 (MQ=60)

      CCGCTTGCGCACGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                       <  SRR18135075.849913/151‑1 (MQ=60)

      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                       <  SRR18135075.3717674/151‑1 (MQ=60)

      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                       <  SRR18135075.6034812/151‑1 (MQ=60)

      CCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCG                                       <  SRR18135075.4635220/151‑1 (MQ=60)

          TGGCGCAAGGAACTCACGGCGCTGGGCGTCCGCCCTCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGG                                   <  SRR18135075.6013893/151‑1 (MQ=59)

          CTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGG                                   <  SRR18135075.6768571/151‑1 (MQ=60)

            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                 <  SRR18135075.2225671/151‑1 (MQ=60)

            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                 <  SRR18135075.5032689/151‑1 (MQ=60)

            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                 <  SRR18135075.9747620/151‑1 (MQ=60)

            GCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGAC                                 >  SRR18135075.5159927/1‑151 (MQ=60)

             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135075.2205638/151‑1 (MQ=60)

             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                >  SRR18135075.275031/1‑151 (MQ=60)

             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                <  SRR18135075.6574317/151‑1 (MQ=60)

             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                >  SRR18135075.9184156/1‑151 (MQ=60)

             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                >  SRR18135075.6844550/1‑151 (MQ=60)

             CGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACC                                >  SRR18135075.6844344/1‑151 (MQ=60)

              GCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCG                               <  SRR18135075.8401390/151‑1 (MQ=60)

              GCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGGGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCG                               <  SRR18135075.330092/151‑1 (MQ=60)

                    AACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGG                         <  SRR18135075.9878055/151‑1 (MQ=60)

                    AACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGG                         >  SRR18135075.4239795/1‑151 (MQ=60)

                    AACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGAAGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGG                         <  SRR18135075.4501442/151‑1 (MQ=60)

                     ACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGA                        >  SRR18135075.3749634/1‑151 (MQ=60)

                     ACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGA                        >  SRR18135075.1355728/1‑151 (MQ=60)

                     ACACACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGA                        <  SRR18135075.9753816/151‑1 (MQ=60)

                      CTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAC                       >  SRR18135075.4356592/1‑151 (MQ=60)

                      CTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGAC                       >  SRR18135075.4356605/1‑151 (MQ=60)

                       TCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACT                      <  SRR18135075.590377/151‑1 (MQ=60)

                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     >  SRR18135075.2826060/1‑151 (MQ=60)

                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     <  SRR18135075.1741913/151‑1 (MQ=60)

                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     <  SRR18135075.1732232/151‑1 (MQ=60)

                        CACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTC                     >  SRR18135075.9377271/1‑151 (MQ=60)

                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                    <  SRR18135075.4773290/151‑1 (MQ=60)

                         ACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCT                    <  SRR18135075.4774618/151‑1 (MQ=60)

                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   <  SRR18135075.9934809/151‑1 (MQ=60)

                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   <  SRR18135075.5342539/151‑1 (MQ=60)

                          CGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTC                   <  SRR18135075.1951432/151‑1 (MQ=60)

                           GGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCG                  >  SRR18135075.9567341/1‑151 (MQ=60)

                            GCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCACCGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGC                 >  SRR18135075.5722691/1‑151 (MQ=60)

                             CGCTGGGCGTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCG                <  SRR18135075.9905406/151‑1 (MQ=60)

                              GCTGTGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGG               >  SRR18135075.10482060/1‑151 (MQ=60)

                              GCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGG               <  SRR18135075.5391835/151‑1 (MQ=60)

                              GCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGG               >  SRR18135075.4608020/1‑151 (MQ=60)

                              GCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGG               >  SRR18135075.4608019/1‑151 (MQ=60)

                              GCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGG               >  SRR18135075.10695074/1‑151 (MQ=60)

                                TGGGCTTCCGCCATCGCCTGTGCTTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCATTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA             <  SRR18135075.210020/151‑1 (MQ=60)

                                TGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGA             >  SRR18135075.4448660/1‑151 (MQ=60)

                                 GGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAG            >  SRR18135075.10705136/1‑151 (MQ=60)

                                 GGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAG            <  SRR18135075.1220479/151‑1 (MQ=60)

                                 GGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAG            <  SRR18135075.1220354/151‑1 (MQ=60)

                                     TTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGG        <  SRR18135075.3016470/151‑1 (MQ=60)

                                     TTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGG        <  SRR18135075.3016471/151‑1 (MQ=60)

                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC       >  SRR18135075.468408/1‑151 (MQ=60)

                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC       >  SRR18135075.9796331/1‑151 (MQ=60)

                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC       >  SRR18135075.680840/1‑151 (MQ=60)

                                      TCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGC       >  SRR18135075.684264/1‑151 (MQ=60)

                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      <  SRR18135075.2232554/151‑1 (MQ=60)

                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      >  SRR18135075.8713307/1‑151 (MQ=60)

                                       CCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCC      <  SRR18135075.7270106/151‑1 (MQ=60)

                                        CGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCG     >  SRR18135075.973946/1‑151 (MQ=60)

                                          CCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTC   <  SRR18135075.8494269/151‑1 (MQ=60)

                                          CCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTC   >  SRR18135075.8370845/1‑151 (MQ=60)

                                           CATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  SRR18135075.186518/1‑151 (MQ=60)

                                           CATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  SRR18135075.8230797/1‑151 (MQ=60)

                                           CATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAGGGTCCTGGCGAAC‑AGCCACGACGGCCTGGCCATCGAGGACGAGACAGCGCTGCAACGCCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  SRR18135075.10432724/1‑151 (MQ=60)

CGAGGCCCGCTTGCGCAAGGAACTCACGGCGCTGGGCTTCCGCCATCGCCTGTGCCTGGCGCCGAACCCGGTGGCGGCGAAGGTCAT‑GCGCACTACACAGGACAACATTTCCATCGAGGACGAGACAGCGCTGCAACACCAGTTGTCCGCCCTTCCGCTGGACCGCCTGGGACTCTCGCGGGAGCTGGCCGTCG  >  NC_008463/4947546‑4947739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`