Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I1 R1 256 492.8 21425088 99.3% 21275112 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,957,532 A→G intergenic (‑354/‑318) PA14_RS22700 ← / → PA14_RS22705 16S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,957,5320AG99.6% 424.6 / NA 223intergenic (‑354/‑318)PA14_RS22700/PA14_RS2270516S ribosomal RNA/exonuclease SbcCD subunit D C‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (105/117);  minor base T (0/1);  total (105/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

TGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTAT  >  NC_008463/4957455‑4957678
                                                                             |                                                                                                                                                  
    ccGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTg                                                                        >  2:890440/1‑150 (MQ=12)
    ccGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTg                                                                        >  1:3036823/1‑150 (MQ=12)
    ccGACCGTCAAACCAGCTTCCGATCAGCGGGAGGCGCAGTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCCCTCCTg                                                                        >  2:6407128/1‑150 (MQ=11)
      gACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTggg                                                                      >  1:7210627/1‑150 (MQ=12)
      gACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCAACATCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCCAACCGCTGCGGCGAACAACCTGCCGGAGCGCAACACCACTCCTggg                                                                      >  1:642356/1‑150 (MQ=11)
       aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                     >  2:2078988/1‑150 (MQ=12)
       aCCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGga                                                                     <  2:8652193/150‑1 (MQ=12)
        ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                    >  2:1709618/1‑150 (MQ=12)
        ccTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgag                                                                    <  2:790149/150‑1 (MQ=12)
         cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                   >  1:1926997/1‑150 (MQ=12)
         cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                   >  1:5304172/1‑150 (MQ=12)
         cTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgaga                                                                   <  1:3911618/150‑1 (MQ=12)
          ttCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagag                                                                  <  1:10309288/150‑1 (MQ=12)
          ttCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagag                                                                  >  2:3638257/1‑150 (MQ=12)
          ttCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagag                                                                  <  1:5829600/150‑1 (MQ=12)
          ttCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGgagag                                                                  >  1:5732215/1‑150 (MQ=12)
           tCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCTCCACTCCTGGgagaga                                                                 >  2:6558803/1‑150 (MQ=255)
            cAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGaa                                                                >  1:4083562/1‑150 (MQ=12)
             aaaCCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAAt                                                               <  1:904564/150‑1 (MQ=12)
               aCCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATcc                                                             >  1:8403355/1‑150 (MQ=12)
                    cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                        <  2:3140882/150‑1 (MQ=12)
                    cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                        <  2:4401540/150‑1 (MQ=12)
                    cTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTaaa                                                        >  2:4032953/1‑150 (MQ=12)
                     ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                       <  2:6185189/150‑1 (MQ=12)
                     ttCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAAt                                                       <  1:684858/150‑1 (MQ=12)
                      tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATc                                                      <  1:2976853/150‑1 (MQ=12)
                      tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATc                                                      <  2:5329616/150‑1 (MQ=12)
                      tCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAAACCGTAAATc                                                      <  2:10695893/150‑1 (MQ=255)
                       ccGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATcc                                                     <  2:4809667/150‑1 (MQ=12)
                        cGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCt                                                    <  1:2575188/150‑1 (MQ=12)
                         gATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCtt                                                   >  1:8234776/1‑150 (MQ=12)
                          aTCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTg                                                  >  1:4310384/1‑150 (MQ=12)
                          aTCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTg                                                  >  1:3325026/1‑150 (MQ=12)
                          aTCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTg                                                  <  1:7653291/150‑1 (MQ=12)
                           tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                 >  1:6469454/1‑150 (MQ=12)
                           tCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGa                                                 >  2:11570/1‑150 (MQ=12)
                            cAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAt                                                <  2:3430300/150‑1 (MQ=12)
                             aGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                               <  1:9011412/150‑1 (MQ=12)
                             aGCGGGAGGCGCATTCTACAGCGTTCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtt                                               <  2:5540858/150‑1 (MQ=255)
                              gCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAttt                                              <  1:9593442/150‑1 (MQ=12)
                               cGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtttt                                             >  2:2179008/1‑150 (MQ=12)
                               cGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGAtttt                                             <  1:2274013/150‑1 (MQ=12)
                                gggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTc                                            >  2:6874450/1‑150 (MQ=12)
                                gggAGGCGCATTCTACAGCGTTCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTc                                            <  2:3786596/150‑1 (MQ=255)
                                 ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                           >  2:7528607/1‑150 (MQ=12)
                                 ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                           >  1:450452/1‑150 (MQ=12)
                                 ggAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCa                                           >  1:9284517/1‑150 (MQ=12)
                                   aGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGATTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAg                                         <  1:4859774/150‑1 (MQ=255)
                                     gcgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCa                                       >  2:2665038/1‑150 (MQ=12)
                                     gcgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCa                                       <  1:2958969/150‑1 (MQ=12)
                                     gcgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCa                                       >  2:7012730/1‑150 (MQ=12)
                                      cgcATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCATCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCAt                                      <  2:3264282/150‑1 (MQ=255)
                                         aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                   <  1:4281448/150‑1 (MQ=12)
                                         aTTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTa                                   <  1:6948905/150‑1 (MQ=12)
                                           tCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTAct                                 <  2:251778/150‑1 (MQ=12)
                                            cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                <  1:9438570/150‑1 (MQ=12)
                                            cTACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActc                                <  1:467873/150‑1 (MQ=12)
                                             tACAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTActct                               <  2:2292807/150‑1 (MQ=12)
                                              aCAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCtt                              <  1:2044436/150‑1 (MQ=12)
                                              aCAGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTGGATTTTCAAGCATTTACTCtt                              >  1:10448602/1‑150 (MQ=255)
                                               cAGCGTTCATATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGTATCCCTTTCGGGACCGAAGCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGTGAGAGAATCCGTTAATCCTTGATTTTCTTGCATTTACTCTtc                             <  1:9892853/150‑1 (MQ=11)
                                                aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                            <  1:10136686/150‑1 (MQ=12)
                                                aGCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcg                            >  1:2245634/1‑150 (MQ=12)
                                                 gCGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgt                           <  2:4746766/150‑1 (MQ=12)
                                                  cGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                          <  2:1430372/150‑1 (MQ=12)
                                                  cGTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtc                          <  1:134722/150‑1 (MQ=12)
                                                   gTTCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcg                         >  2:5015113/1‑150 (MQ=12)
                                                    ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        <  1:3914043/150‑1 (MQ=12)
                                                    ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        <  1:4613154/150‑1 (MQ=12)
                                                    ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        >  2:9189989/1‑150 (MQ=12)
                                                    ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        >  1:8076130/1‑150 (MQ=12)
                                                    ttCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        <  2:1640562/150‑1 (MQ=12)
                                                    ttCAAAGTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgt                        <  2:8499078/150‑1 (MQ=255)
                                                     tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                       >  2:3799120/1‑150 (MQ=12)
                                                     tCAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtc                       >  1:3397414/1‑150 (MQ=12)
                                                      cAAATTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTtcgtcgtcg                      <  1:7114945/150‑1 (MQ=12)
                                                       caagtCGCGGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGc                     <  2:10711205/146‑1 (MQ=11)
                                                        aaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGcc                    >  2:5379251/1‑150 (MQ=12)
                                                        aaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGcc                    >  1:1709112/1‑150 (MQ=12)
                                                        aaTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGcc                    >  2:4187102/1‑150 (MQ=12)
                                                         aTTCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCg                   >  2:7832055/1‑150 (MQ=12)
                                                          ttCGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCgg                  >  2:3194811/1‑150 (MQ=12)
                                                            cGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGa                >  2:7218707/1‑150 (MQ=12)
                                                            cGCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGa                >  1:10614797/1‑150 (MQ=12)
                                                             gCTGTCAAGCCCCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGCGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAg               >  2:10390349/1‑150 (MQ=11)
                                                             gCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAg               <  1:11570/150‑1 (MQ=12)
                                                             gCTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAg               >  1:7686377/1‑150 (MQ=12)
                                                              cTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgt              >  1:6260433/1‑150 (MQ=12)
                                                              cTGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgt              <  2:717719/150‑1 (MQ=12)
                                                               tGTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtg             >  1:9560514/1‑150 (MQ=12)
                                                                gTCAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtgg            <  1:1041868/150‑1 (MQ=12)
                                                                  cAAGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAgtggtg          >  2:2876282/1‑150 (MQ=12)
                                                                   aaGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgc         >  2:9392730/1‑150 (MQ=12)
                                                                    aGCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcg        >  2:8163441/1‑150 (MQ=12)
                                                                     gCACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTgcgc       <  2:1609962/150‑1 (MQ=12)
                                                                      cACCTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCa      <  1:7358969/150‑1 (MQ=12)
                                                                        ccTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAtt    <  1:1809722/150‑1 (MQ=12)
                                                                         cTCGTTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGATCCGCTGCGGCGAACAACCTGCCGGATCGCTTCACCACTCCTGGGAGAGAATCAGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCAttt   <  1:1441493/150‑2 (MQ=11)
                                                                         cTCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATta   <  2:5226566/150‑1 (MQ=12)
                                                                          tCGGTGAAGCTTCGTTTCGAATCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATtat  <  1:868896/150‑1 (MQ=12)
                                                                             |                                                                                                                                                  
TGCGCCGACCTTCAAACCATCTTCCGATCAGCGGGAGGCGCATTCTACAGCGTTCAAATTCGCTGTCAAGCACCTCGATGAAGCTTCGTTTCGAGCCCCTTTCGGGACCGAACCGCTGCGGCGAACAACCTGCCGGAGCGCTTCACCACTCCTGGGAGAGAATCCGTAAATCCTTGATTTTCAAGCATTTACTCTTCGTCGTCGCCGGGAGTGGTGCGCATTAT  >  NC_008463/4957455‑4957678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A