Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
AM260479 |
1,464,441 |
Δ37 bp |
coding (125‑161/552 nt) |
h16_A1354 → |
conserved hypothetical protein |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
AM260479 |
= 1464440 | 2 (0.080) | 34 (1.340) |
22/206 |
0.2 |
97.1% |
coding (124/552 nt) |
h16_A1354 |
conserved hypothetical protein |
| ? | AM260479 |
1464478 = |
0 (0.000) | coding (162/552 nt) |
h16_A1354 |
conserved hypothetical protein |
TCATCAGCTGGTGGGCGAACCGTCCAGCAGTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAACTGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > AM260479/1464337‑1464440
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGGCTGAGCAGGCATGGCCGCGCC > AM260479/1464478‑1464605
TCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTAC > 2:307527/1‑133
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAG < 2:96625/134‑1
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTAC > 1:983639/1‑131
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTAC > 1:96625/1‑131
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGAT > 2:810812/1‑127
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGAT < 1:541166/127‑1
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGAT < 1:810812/127‑1
ATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGAT > 2:541166/1‑127
AGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAG < 1:641957/133‑1
GGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCA > 2:1118729/1‑123
GGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCA < 1:1118729/123‑1
GCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGT < 1:680041/117‑1
GCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGT > 2:680041/1‑117
GAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGG < 1:307527/134‑1
agcGTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCC > 2:133270/4‑134
CCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATA < 1:927146/133‑1
CCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATA < 1:133270/133‑1
ACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCA > 2:105335/1‑99
ACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCA < 1:105335/99‑1
GCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTC < 1:747949/131‑1
CCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGAT < 2:983639/134‑1
ATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAA < 1:283077/134‑1
ACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGCGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGGC > 2:206267/1‑134
TGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGGCTGAG > 1:841749/1‑133
TGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGAT < 1:923232/47‑1
TGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGAT > 2:923232/1‑47
AGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCA > 2:62543/1‑49
AGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCA < 1:62543/49‑1
GGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGG < 2:1115350/101‑1
GGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGG > 1:1115350/1‑101
acagccGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGGCTGAGCAGGCATGGCC > 1:467683/7‑134
ccGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGG > 1:695543/3‑114
ccGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGG < 2:695543/112‑1
cGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGGCTGAGCAGGCATGGCCGCGCC < 2:841749/133‑1
TCATCAGCTGGTGGGCGAACCGTCCAGCAGTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAACTGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > AM260479/1464337‑1464440
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGGGCCCGCGCCCGGTCGACTGGCCTGATAATGACGGCTGAGCAGGCATGGCCGCGCC > AM260479/1464478‑1464605
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCCGTGACAGGAGATGCCATGCACGCCTGTGACCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGCAGTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAACTGCTGGTGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGG > AM260479/1464298‑1464550
|
ggctcggagatgtgtataagagacaGCCTGTGACCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCAC < GWNJ‑0478:712:GW2002102894th:2:1112:17019:29735/125‑1 (MQ=60)
CGTGACAGGAGATGCCATGCACGCCTGTGACCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGG < GWNJ‑0478:712:GW2002102894th:2:1208:15633:46764/150‑1 (MQ=60)
CCTGTGACCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACC > GWNJ‑0478:712:GW2002102894th:2:1107:3148:100132/1‑147 (MQ=60)
CCTGTGACCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACC > GWNJ‑0478:712:GW2002102894th:2:2207:11964:82580/1‑147 (MQ=60)
CCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgc < GWNJ‑0478:712:GW2002102894th:2:1214:7686:32376/150‑3 (MQ=60)
CCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgc < GWNJ‑0478:712:GW2002102894th:2:2115:1904:75520/150‑3 (MQ=60)
ttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgc < GWNJ‑0478:712:GW2002102894th:2:2205:20841:32750/78‑3 (MQ=49)
cagGTCATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcat < GWNJ‑0478:712:GW2002102894th:2:2106:5970:69511/147‑5 (MQ=60)
ggagatgtgtataagagacaggtccagcGTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattggg < GWNJ‑0478:712:GW2002102894th:2:1106:6734:51833/122‑9 (MQ=60)
CATCAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattggg < GWNJ‑0478:712:GW2002102894th:2:2215:15980:84991/149‑9 (MQ=60)
ttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagTGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattggg < GWNJ‑0478:712:GW2002102894th:2:1102:18946:97471/79‑9 (MQ=23)
CAGCTGGTGGGCGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattgggtga < GWNJ‑0478:712:GW2002102894th:2:2104:5257:36844/149‑12 (MQ=60)
CGAACCGTCCAGC‑GTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattgggtgagccgccgaatat < GWNJ‑0478:712:GW2002102894th:2:1203:18477:59194/150‑24 (MQ=60)
GCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattgggtgagccgccgaatatgtggcgaatgggcc < GWNJ‑0478:712:GW2002102894th:2:1109:8981:13057/149‑38 (MQ=60)
GCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGgcattgggtgagccgccgaatatgtggcgaatgggcc < GWNJ‑0478:712:GW2002102894th:2:2205:3700:55691/149‑38 (MQ=60)
GCGGCCATGAGCAACCGGTGCAAGGTCGACAGCCGCTGGCGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCtgtctcttatacacatcccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2105:16141:96930/1‑75 (MQ=59)
|
CGCCGTGACAGGAGATGCCATGCACGCCTGTGACCTGTGTCATCAGCTGGTGGGCGAACCGTCCAGCAGTGCCGCCACACGAGCACCTGGCTGCCTCTGGCATTGCGGCCATGAGCAACCGGTGCAAGGTCGACAACTGCTGGTGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGCTGCACGGGCTGCGGCGCCTGGATGTACCAGAGCACGGCATTGGGTGAGCCGCCGAATATGTGGCGAATGG > AM260479/1464298‑1464550
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
