Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,111,013 |
+G |
coding (553/1089 nt) |
h16_A1942 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,111,012 | 1 | . | G | 100.0%
| 52.5
/ NA
| 15 | D184E (GAT→GAG) | h16_A1942 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (6/9); total (6/9) |
CCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGC > AM260479/2110900‑2111127
|
ccAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc > 2:824275/1‑134 (MQ=255)
cATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGAcg < 1:1082960/134‑1 (MQ=255)
acaAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGc > 1:477888/1‑134 (MQ=255)
aGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCgg > 1:197247/1‑133 (MQ=255)
cgcATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccg < 2:371332/134‑1 (MQ=255)
aTCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccgccg > 1:1048289/1‑134 (MQ=255)
gCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCcgcg < 1:975744/133‑1 (MQ=255)
tGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCcgccgcgcc < 1:122361/85‑1 (MQ=255)
tGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCcgccgcgcc > 2:122361/1‑85 (MQ=255)
ccGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGcgtgccgcg < 2:477888/134‑1 (MQ=255)
aGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTcgc < 1:824275/134‑1 (MQ=255)
gTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTcgcg < 2:197247/134‑1 (MQ=255)
cgacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcgc < 2:744663/133‑1 (MQ=255)
gtggATGGACTCGACGAGGATGCGTTCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGcgcgcc > 1:201960/1‑93 (MQ=255)
gtggATGGACTCGACGAGGATGCGTTCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGcgcgcc < 2:201960/93‑1 (MQ=255)
|
CCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGC > AM260479/2110900‑2111127
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCATTGGGCAGGCCGCGCGTGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGTGCGAACGGCC > AM260479/2110864‑2111154
|
GCATTGGGCAGGCCGCGCGTGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑C > GWNJ‑0478:712:GW2002102894th:2:2103:6891:43170/1‑150 (MQ=60)
gagatgtgtataagagacaGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GG < GWNJ‑0478:712:GW2002102894th:2:1204:8978:91018/131‑1 (MQ=60)
GCCGCGCGTGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGA‑G < GWNJ‑0478:712:GW2002102894th:2:1103:9291:89021/150‑1 (MQ=60)
GCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGC < GWNJ‑0478:712:GW2002102894th:2:2214:14169:11400/150‑1 (MQ=60)
CATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCT > GWNJ‑0478:712:GW2002102894th:2:1213:3687:25300/1‑150 (MQ=60)
CTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGC > GWNJ‑0478:712:GW2002102894th:2:1113:11908:56588/1‑149 (MQ=60)
gtctcgtgggctcggagatgtgtataagagacagATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCAT < GWNJ‑0478:712:GW2002102894th:2:1108:4480:50859/115‑1 (MQ=60)
ttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCG < GWNJ‑0478:712:GW2002102894th:2:1212:19102:67134/71‑1 (MQ=60)
CAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGC > GWNJ‑0478:712:GW2002102894th:2:2212:9735:34533/1‑150 (MQ=60)
GATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑Gctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttg > GWNJ‑0478:712:GW2002102894th:2:2206:15611:85050/1‑83 (MQ=60)
CGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCG < GWNJ‑0478:712:GW2002102894th:2:2207:16353:36225/149‑1 (MQ=60)
GAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGC < GWNJ‑0478:712:GW2002102894th:2:2114:15498:41794/150‑1 (MQ=60)
ACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGTTCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCtgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1113:6255:80773/1‑124 (MQ=60)
gtgtataagagacagggatgGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGC < GWNJ‑0478:712:GW2002102894th:2:2108:10976:77857/130‑1 (MQ=60)
AACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaa > GWNJ‑0478:712:GW2002102894th:2:1116:18859:54216/1‑82 (MQ=60)
GGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGctgtctcttatacacatctccgagcccacgagacactcgctaatct > GWNJ‑0478:712:GW2002102894th:2:1208:6064:77683/1‑104 (MQ=60)
GTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGTGCGAACGGCC > GWNJ‑0478:712:GW2002102894th:2:1110:16574:64699/1‑150 (MQ=60)
|
GCATTGGGCAGGCCGCGCGTGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGTGCGAACGGCC > AM260479/2110864‑2111154
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
