Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,302,920 |
+C |
coding (1199/1251 nt) |
dfp → |
phosphopantothenoylcysteine synthetase/decarboxylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,302,918 | 1 | . | C | 100.0%
| 121.9
/ NA
| 32 | R399R (CGG→CGC) | dfp | phosphopantothenoylcysteine synthetase/decarboxylase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (15/17); total (15/17) |
GGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCC > AM260479/3302795‑3303046
|
ggACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATggc > 2:1131647/1‑134 (MQ=255)
gacAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATggcgggc > 1:1019428/1‑134 (MQ=255)
acAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATggcgggcg > 2:773389/1‑134 (MQ=255)
aCGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGgcgcgc < 2:915162/134‑1 (MQ=255)
cGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCgg > 1:727718/1‑133 (MQ=255)
gTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGcgcgcggcagcc < 1:773389/133‑7 (MQ=255)
tGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCa < 1:123622/133‑1 (MQ=255)
tGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCa > 2:123622/1‑133 (MQ=255)
gACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc < 2:1019428/133‑1 (MQ=255)
gccgGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGc > 1:449106/1‑133 (MQ=255)
ccgGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCa < 2:449106/134‑1 (MQ=255)
ccGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc > 2:769232/1‑102 (MQ=255)
ccGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc < 1:769232/102‑1 (MQ=255)
ccGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTccc > 1:575183/1‑134 (MQ=255)
tCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTc < 1:1131647/132‑1 (MQ=255)
gcgcCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCa < 2:575183/134‑1 (MQ=255)
gcCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGtt > 1:551895/1‑100 (MQ=255)
gcCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGtt < 2:551895/100‑1 (MQ=255)
aGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCAc < 1:1134346/134‑1 (MQ=255)
gATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCaa < 1:253159/130‑1 (MQ=255)
gATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCaa > 2:253159/1‑130 (MQ=255)
cGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTccccc < 2:443541/81‑1 (MQ=255)
cGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTccccc > 1:443541/1‑81 (MQ=255)
ggCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc < 2:444648/45‑1 (MQ=38)
ggCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATgcgc > 1:444648/1‑45 (MQ=38)
cacaCGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTg > 2:610642/1‑46 (MQ=38)
cacaCGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTg < 1:610642/46‑1 (MQ=38)
cacaCGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTAt > 2:419238/1‑54 (MQ=39)
cacaCGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTAt < 1:419238/54‑1 (MQ=39)
cacaCGGCCCTGATGGCGGGCGAGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATgcgcg > 2:480639/1‑134 (MQ=255)
cacGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGcc < 2:727718/134‑1 (MQ=255)
cacGGCCCTGATGGCGGGCGAGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGcc < 1:480639/134‑1 (MQ=255)
|
GGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCC > AM260479/3302795‑3303046
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCCGCC > AM260479/3302784‑3303069
|
ACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCG > GWNJ‑0478:712:GW2002102894th:2:2211:7878:81997/1‑150 (MQ=60)
GGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGT > GWNJ‑0478:712:GW2002102894th:2:2111:17579:39044/1‑149 (MQ=60)
CGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGgcagccgcgtcatgccggcgg < GWNJ‑0478:712:GW2002102894th:2:2113:1814:82456/149‑22 (MQ=60)
GTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCC > GWNJ‑0478:712:GW2002102894th:2:1211:8423:76768/1‑149 (MQ=60)
GCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACC < GWNJ‑0478:712:GW2002102894th:2:1108:6782:58247/150‑1 (MQ=60)
gatgtgtataagagacagCGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTC < GWNJ‑0478:712:GW2002102894th:2:2113:10120:61112/132‑1 (MQ=60)
tttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagaggtgtataagagacagGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTG < GWNJ‑0478:712:GW2002102894th:2:2102:16531:42479/76‑1 (MQ=60)
caagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATT < GWNJ‑0478:712:GW2002102894th:2:1209:13574:18817/84‑1 (MQ=60)
ccaccagctgGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCC > GWNJ‑0478:712:GW2002102894th:2:2101:3597:9954/11‑150 (MQ=60)
caagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaggcCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGAC < GWNJ‑0478:712:GW2002102894th:2:1202:8418:45027/81‑1 (MQ=60)
agattagcgagtgtctcgtgggctcggagatgtgtataagagacagGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCC < GWNJ‑0478:712:GW2002102894th:2:1103:17095:25347/104‑1 (MQ=60)
AGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCctgtctcttatacacatctc > GWNJ‑0478:712:GW2002102894th:2:1214:15951:84118/1‑130 (MQ=60)
GTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCAC < GWNJ‑0478:712:GW2002102894th:2:2216:9285:46228/148‑1 (MQ=60)
CAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCG < GWNJ‑0478:712:GW2002102894th:2:2216:2110:58858/150‑1 (MQ=60)
gtgggctcggagatgtgtataagagacaGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGG < GWNJ‑0478:712:GW2002102894th:2:2112:19152:8714/122‑1 (MQ=60)
GCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGG < GWNJ‑0478:712:GW2002102894th:2:1114:5964:42574/150‑1 (MQ=60)
CGGCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCtgtctcttatacacatctccgagcccacgagacactcg > GWNJ‑0478:712:GW2002102894th:2:1211:6593:48693/1‑112 (MQ=60)
GCCAGCGCCTGCCCGGGCGCACACGGCCCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1211:7103:54297/1‑76 (MQ=60)
ACACGGCCCTGATGGCGGGCGAGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCC < GWNJ‑0478:712:GW2002102894th:2:1213:11875:38938/150‑1 (MQ=60)
GC‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCC > GWNJ‑0478:712:GW2002102894th:2:2112:19597:18488/1‑150 (MQ=60)
GC‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCC > GWNJ‑0478:712:GW2002102894th:2:1108:15216:71949/1‑150 (MQ=60)
CTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATctgtctcttatacacatc > GWNJ‑0478:712:GW2002102894th:2:1104:11527:49487/1‑132 (MQ=60)
|
ACCTTCGGCCTGGACGACAACGAAATCGTGCTGTTCGACGCCGCCGGCATGACGCGGCTGCCGCGCGCCGACAAGCTGTCACTGGCGCGCCAGCTGGTGGCGGCGATCGGCCAGCGCCTGCCCGGGCGCACACGG‑CCTGATGGCGGGCGCGCGTCCGGTATCATGCGCGGCTGCCACCTATTCCGTTGAACCGATTTCCCCCAGGATTCCCATGACCCAGCCACTGAACCCAACCGTCGAAATCAAGGTGCTCGATGCGCGCCTGAATGAATGGGGCCTGCCCGCC > AM260479/3302784‑3303069
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
