Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
3,615,722 |
+A |
intergenic (+48/‑141) |
prfA → / → h16_A3341 |
protein chain release factor A (RF‑1)/Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 3,615,720 | 1 | . | A | 100.0%
| 121.4
/ NA
| 32 | intergenic (+46/‑143) | prfA/h16_A3341 | protein chain release factor A (RF‑1)/Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (16/16); total (16/16) |
ATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCG > AM260479/3615600‑3615840
|
aTGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGa > 1:860571/1‑133 (MQ=255)
gAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCttt > 1:405733/1‑134 (MQ=255)
tCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCtt < 1:1050981/134‑1 (MQ=255)
cGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCtt < 2:506002/133‑1 (MQ=255)
cGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCtt > 1:506002/1‑133 (MQ=255)
aCTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTaaa < 1:295592/134‑1 (MQ=255)
gAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAAc < 2:860571/134‑1 (MQ=255)
aaCACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGaaa > 2:167314/1‑132 (MQ=255)
ccAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCAc < 2:405733/134‑1 (MQ=255)
cTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTa > 1:999158/1‑103 (MQ=255)
cTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTa < 2:999158/103‑1 (MQ=255)
gCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCtt > 1:415429/1‑92 (MQ=255)
gCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCtt < 2:415429/92‑1 (MQ=255)
gCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGt > 2:271405/1‑132 (MQ=255)
cTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 1:167314/134‑1 (MQ=255)
tgcACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 1:481975/1‑129 (MQ=255)
tgcACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 2:481975/129‑1 (MQ=255)
caAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAAc > 1:953599/1‑134 (MQ=255)
caAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAAc > 2:314902/1‑134 (MQ=255)
aaaTGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACg > 2:180511/1‑133 (MQ=255)
gTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATgg > 1:927463/1‑134 (MQ=255)
tGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt > 1:660515/1‑68 (MQ=255)
tGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCtt < 2:660515/68‑1 (MQ=255)
cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCtt < 1:339775/56‑1 (MQ=255)
cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCtt > 2:339775/1‑56 (MQ=255)
cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg > 2:431276/1‑89 (MQ=255)
cAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAg < 1:431276/89‑1 (MQ=255)
cGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCggg < 1:314902/134‑1 (MQ=255)
gCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTa < 1:271405/134‑1 (MQ=255)
tCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTa > 2:649939/1‑130 (MQ=255)
tCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTa < 1:649939/130‑1 (MQ=255)
ccGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCg < 2:953599/134‑1 (MQ=255)
|
ATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCG > AM260479/3615600‑3615840
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAACATACCTAGG > AM260479/3615585‑3615854
|
ATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATC > GWNJ‑0478:712:GW2002102894th:2:1107:13361:90042/1‑150 (MQ=60)
ATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGAT > GWNJ‑0478:712:GW2002102894th:2:2201:12373:37560/1‑149 (MQ=60)
GACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTG > GWNJ‑0478:712:GW2002102894th:2:1208:12026:46249/1‑150 (MQ=60)
TGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGT > GWNJ‑0478:712:GW2002102894th:2:1212:14514:65472/1‑150 (MQ=60)
agcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAAC < GWNJ‑0478:712:GW2002102894th:2:1205:7856:61693/86‑1 (MQ=60)
GTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAAC < GWNJ‑0478:712:GW2002102894th:2:2212:5831:48490/150‑1 (MQ=60)
CACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCAC < GWNJ‑0478:712:GW2002102894th:2:1201:19525:84318/150‑1 (MQ=60)
CTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTctgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:2208:3976:70981/1‑133 (MQ=60)
aggttatctattgtgtttttcaagcagaagacggcatacgagattagcgagtgtctcgtggggctcggagatgtgtataagagacagccgcgtaAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGT < GWNJ‑0478:712:GW2002102894th:2:2103:7532:82502/56‑1 (MQ=60)
ACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAActgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:1208:17720:97566/1‑122 (MQ=60)
GGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCT > GWNJ‑0478:712:GW2002102894th:2:1213:5036:45528/1‑150 (MQ=60)
GCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAAT < GWNJ‑0478:712:GW2002102894th:2:1111:11993:96752/150‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagCCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAAT < GWNJ‑0478:712:GW2002102894th:2:1209:6721:84358/119‑1 (MQ=60)
aagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagcccgaacaccgcgtaAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCG < GWNJ‑0478:712:GW2002102894th:2:1211:5188:42224/76‑1 (MQ=60)
GAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACG > GWNJ‑0478:712:GW2002102894th:2:2206:20568:5949/1‑150 (MQ=60)
GCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGT > GWNJ‑0478:712:GW2002102894th:2:2205:14159:57403/1‑150 (MQ=60)
CACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTActgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatg > GWNJ‑0478:712:GW2002102894th:2:2107:10158:51426/1‑98 (MQ=60)
cgtgggctcggagatgtgtataagagacaGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGG < GWNJ‑0478:712:GW2002102894th:2:1113:8860:22804/118‑1 (MQ=60)
CCGGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGA < GWNJ‑0478:712:GW2002102894th:2:1202:7655:6283/150‑1 (MQ=60)
aagagacagGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGA < GWNJ‑0478:712:GW2002102894th:2:1112:8202:68542/140‑1 (MQ=60)
GGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAAC < GWNJ‑0478:712:GW2002102894th:2:1116:12957:34660/150‑1 (MQ=60)
GGCCTTCCGCCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAAC < GWNJ‑0478:712:GW2002102894th:2:2104:15061:87177/150‑1 (MQ=60)
CCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaaacacaaaac > GWNJ‑0478:712:GW2002102894th:2:2114:19441:83688/1‑62 (MQ=60)
CCCCGAACACCGCGTAAAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAACATACCTAGG > GWNJ‑0478:712:GW2002102894th:2:1110:20176:21840/1‑150 (MQ=60)
|
ATCATGGACGGCGACATGGATGAGCTGCTGTCGGCACTGGCTTCGGAACACCAGGCTGATCAGCTTGCTGCACTTGGCGAAGAGTCGTAAGCACCACAAAATGTCGTGCAACCGGCCTTCCGCCCCGAACA‑CGCGT‑AAGTCGCCGATATCTTTGTATTTCTTGTAAATGCTTGAAACAGCACTGGTAGGGGTAAGGCGGCTGTCTATAATCGATTTCGACCGGCAAACGGAGAATGGTGCCGGGTAGTCACTCCCGAGAACATACCTAGG > AM260479/3615585‑3615854
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
