Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
155 |
25.8 |
2285122 |
88.0% |
2010907 |
104.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
978,518 |
A→G |
S117G (AGC→GGC) |
h16_B0862 → |
cytosine deaminase or related metal‑dependent hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 978,518 | 0 | A | G | 100.0%
| 107.7
/ NA
| 29 | S117G (AGC→GGC) | h16_B0862 | cytosine deaminase or related metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/15); total (14/15) |
CCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAAGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCG > AM260480/978414‑978620
|
ccGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAggcagc < 1:1125875/110‑1 (MQ=255)
ccGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAggcagc > 2:1125875/1‑110 (MQ=255)
gCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTgtcg > 2:244612/1‑134 (MQ=255)
gATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAg > 2:862779/1‑109 (MQ=255)
gATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAg > 2:731649/1‑109 (MQ=255)
gATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAg < 1:862779/109‑1 (MQ=255)
gATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAg < 1:731649/109‑1 (MQ=255)
cAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTgcg < 1:244612/134‑1 (MQ=255)
gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg > 2:324229/1‑80 (MQ=255)
gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg < 2:345662/80‑1 (MQ=255)
gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg < 2:462465/80‑1 (MQ=255)
gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg > 1:462465/1‑80 (MQ=255)
gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg > 1:345662/1‑80 (MQ=255)
gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg < 1:324229/80‑1 (MQ=255)
cAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTgcg > 1:420237/1‑123 (MQ=255)
cAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTgcg < 2:420237/123‑1 (MQ=255)
aaGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCa > 1:396818/1‑80 (MQ=255)
aaGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCa < 2:396818/80‑1 (MQ=255)
ggTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTg < 2:235675/61‑1 (MQ=255)
ggTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTg > 1:235675/1‑61 (MQ=255)
gCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCt < 2:960061/103‑1 (MQ=255)
gCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCt > 1:960061/1‑103 (MQ=255)
cGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTcacc < 1:626864/118‑1 (MQ=255)
cGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTcacc > 2:626864/1‑118 (MQ=255)
agaTCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGc < 2:759916/134‑1 (MQ=255)
gcgcGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCacctgt < 2:1139929/47‑1 (MQ=255)
gcgcGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCacctgt > 1:1139929/1‑47 (MQ=255)
ggTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCg < 2:294953/116‑1 (MQ=255)
ggTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCg > 1:294953/1‑116 (MQ=255)
tGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCg > 2:112290/1‑84 (MQ=255)
tGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCg < 1:112290/84‑1 (MQ=255)
|
CCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAAGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCG > AM260480/978414‑978620
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCTGCCGCGCATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAAGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCGGTCGCGCGCGCGGTCGCCGATG > AM260480/978390‑978659
|
aagagacagGCATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCAC < GWNJ‑0478:712:GW2002102894th:2:2216:13482:18922/140‑1 (MQ=60)
ataagagacagGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTAC < GWNJ‑0478:712:GW2002102894th:2:2111:4115:59811/139‑1 (MQ=60)
ataagagacagGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTAC < GWNJ‑0478:712:GW2002102894th:2:2201:8853:52232/139‑1 (MQ=60)
gcgagtgtctcgtgggctcggagatgtgtataagagacagCCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACC < GWNJ‑0478:712:GW2002102894th:2:1202:2245:65987/110‑1 (MQ=60)
CCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCctgtctcttatacacatctccgagcccacgagacactcgc > GWNJ‑0478:712:GW2002102894th:2:1205:5696:96260/1‑110 (MQ=60)
CCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCctgtctcttatacacatctccgagcccacgagacactcgc > GWNJ‑0478:712:GW2002102894th:2:1210:2531:46965/1‑110 (MQ=60)
GACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑AC > GWNJ‑0478:712:GW2002102894th:2:1209:5224:24288/1‑150 (MQ=60)
ACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCtgtctcttatacacatctccgagcccacgagacactcgc > GWNJ‑0478:712:GW2002102894th:2:1206:7730:95937/1‑111 (MQ=60)
GCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCAC < GWNJ‑0478:712:GW2002102894th:2:1115:11417:99336/150‑1 (MQ=60)
GTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGctgtctcttatacacatctccgagcccacgagacactcgctaatatcgtatgcagtctt > GWNJ‑0478:712:GW2002102894th:2:1115:20486:53611/1‑91 (MQ=60)
AGGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCACCGTGGTCGATGCC < GWNJ‑0478:712:GW2002102894th:2:2105:21191:43879/150‑1 (MQ=60)
AGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCtgtctcttatacacat > GWNJ‑0478:712:GW2002102894th:2:2206:6863:43542/1‑134 (MQ=60)
gtgtctcgtgggctcggagatgtgtataagagacagGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCACCGTGGTCGATGCCGGCA < GWNJ‑0478:712:GW2002102894th:2:1106:9103:91845/114‑1 (MQ=60)
ATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaa > GWNJ‑0478:712:GW2002102894th:2:2216:13965:87023/1‑77 (MQ=60)
CTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCTGT > GWNJ‑0478:712:GW2002102894th:2:1201:20652:80169/1‑150 (MQ=60)
GTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGTGCCGGCATCGACCACGGTGGT‑GAAGCCG‑‑CCGCGCAGCGACGCCGGGCTGGATGCGGTCGCGCGCGCG > GWNJ‑0478:712:GW2002102894th:2:2211:2046:69314/1‑150 (MQ=59)
GCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCatcgaccacggtggtgaagccctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgc > GWNJ‑0478:712:GW2002102894th:2:1106:13596:77836/1‑76 (MQ=60)
|
CGCTGCCGCGCATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAAGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGC‑‑GGCGGCTTC‑ACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCGGTCGCGCGCGCGGTCGCCGATG > AM260480/978390‑978659
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
