Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 2
2 1 3
3 1 3
4 1 3
5 1 3
6 1 1
7 1 3
8 1 2
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 419 832
Multiple Base Substitutions 1 1
Deletions 19 64
Insertions 22 44
Mobile Element Insertions 12 26
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 473 967

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 14 5 46.8 1456587 99.0% 1442021 150.2
A1 F50 I2 R1 14 6 50.4 1586623 98.1% 1556477 150.0
A2 F50 I1 R1 14 5 56.9 1764756 99.2% 1750637 150.2
A2 F50 I2 R1 12 4 73.2 2275784 99.4% 2262129 150.1
A2 F50 I3 R1 12 5 64.6 2041532 97.6% 1992535 150.0
A3 F50 I1 R1 300 5 54.7 1718999 98.4% 1691495 149.9
A3 F50 I2 R1 257 6 49.9 1554294 99.0% 1538751 150.0
A3 F50 I3 R1 283 5 58.3 1819865 98.8% 1798026 150.1
A4 F50 I1 R1 14 4 47.9 1492993 99.0% 1478063 150.2
A4 F50 I2 R1 11 4 44.6 1622634 97.7% 1585313 150.1
A4 F50 I3 R1 9 4 53.9 1676598 99.1% 1661508 150.2
A5 F50 I1 R1 12 4 45.0 1637969 98.8% 1618313 150.2
A5 F50 I2 R1 13 4 61.7 1951856 97.4% 1901107 150.0
A5 F50 I3 R1 9 4 69.6 2172233 98.7% 2143993 150.2
A6 F50 I1 R1 12 6 52.1 1626478 98.8% 1606960 150.1
A7 F50 I1 R1 16 5 44.5 1394873 98.4% 1372555 150.1
A7 F50 I2 R1 14 5 51.3 1601205 98.8% 1581990 150.1
A7 F50 I3 R1 16 5 56.1 1756558 98.6% 1731966 150.0
A8 F50 I1 R1 11 3 53.7 1782821 99.2% 1768558 150.1
A8 F50 I2 R1 12 3 72.3 2250417 99.2% 2232413 150.1

Mutation Needle Plot

Mutation Targets

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