breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 13,865 C→T A568V (GCG→GTG)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ
RA 64,950 A→G G277G (GGT→GGC polB ← DNA polymerase II
RA 74,829 Δ1 bp coding (652/984 nt) thiB ← thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
RA 93,668 A→G E168G (GAG→GGG)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
RA 97,947 T→C R287R (CGT→CGC murD → UDP‑N‑acetylmuramoyl‑L‑alanine:D‑glutamate ligase
RA 104,664 A→G H228R (CAC→CGC)  ftsA → ATP‑binding cell division FtsK recruitment protein
RA 146,238 A→G K386K (AAA→AAG yadE → putative polysaccharide deacetylase lipoprotein
RA 150,128 A→G S276P (TCT→CCT)  yadC ← putative fimbrial‑like adhesin protein
RA 170,771 G→A R66H (CGC→CAC)  fhuD → iron(3+)‑hydroxamate import ABC transporter periplasmic binding protein
RA 203,087 T→C G176G (GGT→GGC lpxA → UDP‑N‑acetylglucosamine acetyltransferase
RA 233,559 T→C T133A (ACG→GCG)  mltD ← putative membrane‑bound lytic murein transglycosylase D
MC JC 257,908 Δ776 bp [crl] [crl]
RA 272,712 (G)5→6 intergenic (+523/‑135) insN → / → ykfC pseudogene, IS911 transposase A;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/CP4‑6 prophage; conserved protein;Phage or Prophage Related
RA 300,337 C→T D200N (GAT→AAT)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 306,996 G→A N779N (AAC→AAT ecpC ← ECP production outer membrane protein
RA 308,755 C→T S193N (AGC→AAC)  ecpC ← ECP production outer membrane protein
RA 327,536 A→G S400P (TCG→CCG)  betB ← betaine aldehyde dehydrogenase, NAD‑dependent
RA 327,990 T→C E248E (GAA→GAG betB ← betaine aldehyde dehydrogenase, NAD‑dependent
RA 341,378 A→G Q85R (CAG→CGG)  yahJ → putative metallo‑dependent hydrolase domain deaminase
RA 358,347 G→A A115V (GCG→GTG)  cynR ← transcriptional activator of cyn operon; autorepressor
RA 359,143 T→C H115H (CAT→CAC cynT → carbonic anhydrase
RA 373,704 G→A V262M (GTG→ATG)  mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding
RA 396,691 T→C V18A (GTT→GCT)  sbmA → peptide antibiotic transporter
RA 398,614 A→G N248S (AAC→AGC)  yaiW → microcin Bac7 uptake protein; outer membrane surface‑exposed lipoprotein
RA 411,745 A→G L246P (CTA→CCA)  araJ ← L‑arabinose‑inducible putative transporter, MFS family
RA 413,307 T→C D816G (GAC→GGC)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 443,420 A→G G74G (GGT→GGC yajL ← oxidative‑stress‑resistance chaperone
RA 455,731 A→G D200G (GAC→GGC)  tig → peptidyl‑prolyl cis/trans isomerase (trigger factor)
RA 483,728 Δ1 bp coding (676/3150 nt) acrB ← multidrug efflux system protein
RA 485,845 (G)5→6 coding (85/648 nt) acrR → transcriptional repressor
RA 505,708 (A)6→7 coding (795/1653 nt) ushA → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA 514,375 G→A G128D (GGC→GAC)  cueR → copper‑responsive regulon transcriptional regulator
RA 541,599 A→G L345L (TTA→TTG ybbY → putative uracil/xanthine transporter
RA 548,053 A→G E458E (GAA→GAG fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 549,698 A→G G24G (GGA→GGG ylbF → putative anaerobic allantoin catabolic oxamate carbamoyltransferase; DUF2877 family protein
RA 563,150 A→G N272S (AAC→AGC)  sfmH → FimA homolog, function unknown
RA 597,758 (G)5→6 coding (280/1224 nt) cusB → copper/silver efflux system, membrane fusion protein
RA 642,943 A→G S286G (AGC→GGC)  ybdR → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 663,381 C→T A195T (GCG→ACG)  dacA ← D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
RA 686,137 T→C N178S (AAC→AGC)  gltJ ← glutamate/aspartate ABC transporter permease
RA 699,862 A→G A155A (GCT→GCC umpH ← UMP phosphatase
RA 701,461 (G)5→6 coding (134/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
RA 709,902 A→G N38S (AAC→AGC)  chiQ → chitosugar‑induced verified lipoprotein
RA 729,072 A→G intergenic (‑251/‑62) kdpF ← / → ybfA potassium ion accessory transporter subunit/DUF2517 family protein
RA 737,765 A→G pseudogene (941/1137 nt) ybfL → pseudogene, DDE domain transposase family;putative factor; Not classified; putative receptor protein
JC JC 752,861 IS5 (+) +4 bp intergenic (‑66/+321) ybgD ← / ← gltA putative fimbrial‑like adhesin protein/citrate synthase
RA 766,588 C→T A202V (GCT→GTT)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 768,591 T→C V205A (GTG→GCG)  mngB → alpha‑mannosidase
RA 776,165 T→G V106G (GTC→GGC)  tolR → membrane spanning protein in TolA‑TolQ‑TolR complex
RA 777,573 T→C V411A (GTG→GCG)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex
RA 780,929 C→T intergenic (+11/‑139) lysW → / → valZ tRNA‑Lys/tRNA‑Val
RA 787,474 G→A A41V (GCA→GTA)  gpmA ← phosphoglyceromutase 1
RA 798,688 C→T P35S (CCG→TCG)  pgl → 6‑phosphogluconolactonase
RA 811,250 A→G A290A (GCA→GCG bioF → 8‑amino‑7‑oxononanoate synthase
RA 829,895 A→G Y26Y (TAT→TAC ybhG ← putative membrane fusion protein (MFP) component of efflux pump, membrane anchor
RA 832,662 T→C M97V (ATG→GTG)  ybiA ← DUF1768 family protein
RA 846,072 T→C H131R (CAT→CGT)  glnQ ← glutamine transporter subunit
RA 855,061 A→G G228G (GGT→GGC ldtB ← L,D‑transpeptidase linking Lpp to murein
RA 868,945 C→T R476C (CGC→TGC)  gsiA → glutathione ABC transporter ATPase
RA 902,979 C→T V4I (GTT→ATT)  artI ← arginine transporter subunit
RA 904,502 T→C intergenic (‑35/‑91) ybjP ← / → ybjQ lipoprotein/UPF0145 family protein
RA 915,205 C→T intergenic (‑348/+147) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 915,916 A→G G44G (GGT→GGC aqpZ ← aquaporin Z
RA 924,776 A→G T505A (ACG→GCG)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 925,392 A→G D710G (GAC→GGC)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 944,703 G→A G224D (GGT→GAT)  dmsC → dimethyl sulfoxide reductase, anaerobic, subunit C
RA 964,931 A→G E204E (GAA→GAG ycaI → ComEC family inner membrane protein
RA 969,078 T→C I238T (ATT→ACT)  lpxK → lipid A 4'kinase
RA 973,216 C→T L62L (CTG→CTA elyC ← envelope biogenesis factor; DUF218 superfamily protein
RA 982,139 C→T R365* (CGA→TGA)  ldtD → murein L,D‑transpeptidase
RA 1,002,538 A→G V244V (GTA→GTG elfG → putative fimbrial‑like adhesin protein
RA 1,010,983 C→T A340A (GCC→GCT uup → replication regulatory ABC‑F family DNA‑binding ATPase
RA 1,022,227 A→G Y553H (TAT→CAT)  yccS ← putative transporter, FUSC superfamily inner membrane protein
RA 1,028,234 A→G T97A (ACT→GCT)  yccU → putative CoA‑binding protein
RA 1,042,663 A→G R516R (CGT→CGC etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation
RA 1,048,639 G→A P18L (CCC→CTC)  gfcC ← putative O‑antigen capsule production periplasmic protein
RA 1,072,718 A→G V49A (GTC→GCC)  rutB ← ureidoacrylate amidohydrolase
RA 1,119,351 C→T G33R (GGA→AGA)  yceO ← uncharacterized protein
RA 1,148,867 A→G T37A (ACC→GCC)  fabH → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
RA 1,151,418 A→G intergenic (+14/‑197) fabG → / → acpP 3‑oxoacyl‑[acyl‑carrier‑protein] reductase/acyl carrier protein (ACP)
RA 1,174,596 A→G S190S (AGT→AGC ycfT ← inner membrane protein
RA 1,192,714 A→G C354R (TGC→CGC)  mnmA ← tRNA(Gln,Lys,Glu) U34 2‑thiouridylase
RA 1,212,088 (C)8→9 intergenic (‑85/+615) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
RA 1,217,003 A→G intergenic (+7/‑325) ymgC → / → ycgG Blue light, low temperature and stress induced protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 1,225,048 A→G V105A (GTT→GCT)  minD ← inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
RA 1,232,002 C→T R273W (CGG→TGG)  umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity
RA 1,237,152 T→C A30A (GCA→GCG ycgB ← SpoVR family stationary phase protein
RA 1,238,031 T→C I154T (ATC→ACC)  dadA → D‑amino acid dehydrogenase
RA 1,260,849 G→A intergenic (‑46/+79) dauA ← / ← prs C4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase
RA 1,265,331 A→G D107G (GAC→GGC)  prfA → peptide chain release factor RF‑1
RA 1,266,451 A→G T120A (ACC→GCC)  prmC → RF‑1 and RF‑2 N5‑glutamine methyltransferase
RA 1,302,933 C→T A12V (GCG→GTG)  oppB → oligopeptide ABC transporter permease
RA 1,313,733 A→G intergenic (‑70/‑287) yciC ← / → ompW UPF0259 family inner membrane protein/outer membrane protein W
RA 1,318,202 T→C T72A (ACG→GCG)  trpB ← tryptophan synthase, beta subunit
RA 1,333,046 A→G T667A (ACG→GCG)  topA → DNA topoisomerase I, omega subunit
RA 1,336,703 T→C G291G (GGT→GGC acnA → aconitate hydratase 1; aconitase A
RA 1,351,196 (G)7→8 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,369,520 T→C V43A (GTT→GCT)  pspD → peripheral inner membrane phage‑shock protein
RA 1,373,362 T→C N47N (AAT→AAC ycjO → putative sugar ABC transporter permease
RA 1,381,934 A→G intergenic (+32/‑13) ycjV → / → ompG pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G
RA 1,417,189 A→G A66A (GCT→GCC recE ← Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease
RA 1,420,861 A→G K60E (AAA→GAA)  ydaT → Rac prophage; uncharacterized protein
RA 1,430,519 T→C S491P (TCA→CCA)  stfR → Rac prophage; putative tail fiber protein
RA 1,473,811 A→G pseudogene (3295/3497 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,474,495 A→G D92G (GAC→GGC)  ydbC → putative NAD(P)‑binding oxidoreductase
RA 1,486,239 T→C L1060P (CTG→CCG)  hrpA → putative ATP‑dependent helicase
RA 1,526,410 C→T T55I (ACA→ATA)  yncG → glutathione S‑transferase homolog
RA 1,559,761 T→C S50S (TCA→TCG ddpC ← D,D‑dipeptide ABC transporter permease
RA 1,582,048 A→G G159G (GGT→GGC ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase
RA 1,583,067 A→G C207C (TGT→TGC ydeO ← UV‑inducible global regulator, EvgA‑, GadE‑dependent
RA 1,586,995 C→T G247D (GGT→GAT)  ydeQ ← putative fimbrial‑like adhesin protein
RA 1,594,256 (C)8→9 pseudogene (3732/3861 nt) yneO ← pseudogene, AidA homolog
RA 1,604,698 A→G T218A (ACG→GCG)  lsrD → autoinducer 2 import system permease protein
RA 1,608,413 T→C T204A (ACG→GCG)  yneE ← bestrophin family putative inner membrane protein
RA 1,609,216 A→G intergenic (‑194/+13) yneE ← / ← uxaB bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent
RA 1,612,510 C→T G248R (GGG→AGG)  glsB ← glutaminase 2
RA 1,618,274 C→T G212D (GGC→GAC)  marC ← UPF0056 family inner membrane protein
RA 1,618,445 A→G V155A (GTA→GCA)  marC ← UPF0056 family inner membrane protein
RA 1,621,321 C→T intergenic (‑184/‑11) eamA ← / → ydeE cysteine and O‑acetyl‑L‑serine efflux system/putative transporter
RA 1,632,369 T→C intergenic (‑84/‑703) ydfJ ← / → ydfK pseudogene, MFS transporter family; interrupted by Qin prophage;Phage or Prophage Related; putative transport protein/cold shock protein, function unknown, Qin prophage
RA 1,644,276 A→G V10A (GTG→GCG)  ydfU ← Qin prophage; uncharacterized protein
RA 1,659,314 A→G T416A (ACC→GCC)  ynfE → putative selenate reductase, periplasmic
RA 1,663,917 G→A V103V (GTG→GTA ynfH → oxidoreductase, membrane subunit
RA 1,680,390 A→G G278G (GGA→GGG ydgI → putative arginine/ornithine antiporter transporter
RA 1,711,501 A→G N225S (AAC→AGC)  rsxE → SoxR iron‑sulfur cluster reduction factor component; electron transport inner membrane NADH‑quinone reductase
RA 1,727,494 (G)6→7 coding (836/1098 nt) nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
RA 1,751,240 T→C E162G (GAA→GGA)  ydhW ← FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
RA 1,781,206 G→A G269D (GGC→GAC)  ydiR → putative electron transfer flavoprotein, FAD‑binding subunit
RA 1,781,351 A→G intergenic (+12/‑44) ydiR → / → ydiS putative electron transfer flavoprotein, FAD‑binding subunit/putative oxidoreductase
RA 1,790,475 T→C Y257C (TAC→TGC)  ydiU ← UPF0061 family protein
RA 1,802,726 A→G intergenic (‑156/‑368) thrS ← / → arpB threonyl‑tRNA synthetase/pseudogene, ankyrin repeats
RA 1,806,485 (G)5→4 coding (116/930 nt) pfkB → 6‑phosphofructokinase II
RA 1,812,657 (T)7→8 coding (476/804 nt) ydjO ← uncharacterized protein
RA 1,821,936 A→T D107E (GAT→GAA osmE ← osmotically‑inducible lipoprotein
RA 1,824,020 T→C L169P (CTG→CCG)  cho → endonuclease of nucleotide excision repair
RA 1,829,705 T→C I10M (ATA→ATG astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,859,148 A→G F361S (TTC→TCC)  ydjK ← putative MFS sugar transporter, membrane protein
RA 1,905,571 T→C intergenic (+312/‑117) yobD → / → mntP UPF0266 family inner membrane protein/putative Mn(2+) efflux pump, mntR‑regulated
RA 1,908,423 C→T Q42Q (CAG→CAA yebO ← putative inner membrane protein
RA 1,938,397 G→A R250H (CGT→CAT)  pykA → pyruvate kinase II
RA 1,941,228 A→G G136G (GGT→GGC mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein
RA 1,953,303 T→C M201T (ATG→ACG)  cmoA → carboxy‑SAM synthase
RA 1,967,611 (C)5→6 coding (891/1050 nt) cheB ← fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,009,257 T→C G327G (GGT→GGC yedE → UPF0394 family sulphur transport domain‑containing inner membrane protein
RA 2,010,754 T→C V52A (GTA→GCA)  yedL → GNAT family putative N‑acetyltransferase
RA 2,059,573 G→A R40C (CGC→TGC)  yeeO ← putative multdrug exporter, MATE family
RA 2,077,754 G→A A62A (GCG→GCA cbtA → CP4‑44 prophage; toxin of the YeeV‑YeeU toxin‑antitoxin system
RA 2,082,465 G→A S28L (TCA→TTA)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
RA 2,104,750 T→C H112R (CAC→CGC)  wbbJ ← putative lipopolysaccharide biosynthesis O‑acetyl transferase
RA 2,152,439 C→T G12R (GGA→AGA)  yegK ← ser/thr phosphatase‑related protein
RA 2,158,566 T→C S61P (TCT→CCT)  mdtC → multidrug efflux system, subunit C
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,182,939 T→C D238G (GAC→GGC)  yegX ← putative family 25 glycosyl hydrolase
RA 2,209,276 G→A pseudogene (177/2001 nt) yehQ → pseudogene
RA 2,209,868 A→G pseudogene (769/2001 nt) yehQ → pseudogene
RA 2,236,037 G→A G258D (GGT→GAT)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
RA 2,240,292 G→A A19A (GCC→GCT mglB ← methyl‑galactoside transporter subunit
RA 2,243,790 T→C intergenic (‑138/‑120) folE ← / → yeiG GTP cyclohydrolase I/S‑formylglutathione hydrolase
RA 2,246,914 T→C intergenic (‑145/+149) cirA ← / ← lysP colicin IA outer membrane receptor and translocator; ferric iron‑catecholate transporter/lysine transporter
RA 2,251,116 G→A D93N (GAT→AAT)  nfo → endonuclease IV with intrinsic 3'‑5' exonuclease activity
RA 2,265,230 A→G G22G (GGT→GGC yeiW ← UPF0153 cysteine cluster protein
RA 2,268,435 G→A A203T (GCC→ACC)  yeiR → Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
RA 2,270,278 T→C R100R (CGT→CGC mepS → murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase
RA 2,276,922 T→C M208T (ATG→ACG)  yejF → microcin C ABC transporter ATPase
RA 2,281,016 C→T Q129* (CAG→TAG)  yejH → putative ATP‑dependent DNA or RNA helicase
RA 2,304,235 A→G K111E (AAG→GAG)  eco → ecotin, a serine protease inhibitor
RA 2,326,925 (C)6→7 coding (817/1185 nt) atoB → acetyl‑CoA acetyltransferase
RA 2,348,587 C→T A25V (GCG→GTG)  yfaE → ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance
RA 2,398,634 T→C Q511R (CAG→CGG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,401,795 G→A R86R (CGC→CGT nuoE ← NADH:ubiquinone oxidoreductase, chain E
RA 2,420,100 G→A A129V (GCG→GTG)  yfcF ← glutathione S‑transferase
RA 2,437,826 A→G M9T (ATG→ACG)  pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,445,152 G→A R137C (CGC→TGC)  yfcA ← TauE/TSUP family inner membrane protein
RA 2,459,668 T→C T268A (ACG→GCG)  fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
RA 2,478,163 T→C V106A (GTG→GCG)  dsdX → D‑serine transporter
RA 2,478,551 A→G I235M (ATA→ATG dsdX → D‑serine transporter
RA 2,488,952 T→C S73G (AGT→GGT)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,502,083 A→G R801R (CGT→CGC fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,502,713 C→T L591L (CTG→CTA fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,527,658 T→C V238A (GTT→GCT)  yfeH → putative inorganic ion transporter
RA 2,528,926 A→G R417R (CGT→CGC ligA ← DNA ligase, NAD(+)‑dependent
RA 2,558,660 A→G L7S (TTG→TCG)  eutB ← ethanolamine ammonia‑lyase, large subunit, heavy chain
RA 2,567,770 G→A A113V (GCG→GTG)  eutH ← ethanolamine transporter
RA 2,576,775 T→C N535D (AAC→GAC)  maeB ← malic enzyme: putative oxidoreductase/phosphotransacetylase
RA 2,579,823 A→G N63S (AAC→AGC)  tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,581,825 T→C Q318R (CAG→CGG)  ypfG ← DUF1176 family protein
RA 2,602,723 G→A R302R (CGG→CGA hyfB → hydrogenase 4, membrane subunit
RA 2,607,998 T→C V361A (GTA→GCA)  hyfF → hydrogenase 4, membrane subunit
RA 2,627,887 (G)5→6 coding (1052/2244 nt) yfgF ← cyclic‑di‑GMP phosphodiesterase, anaerobic
RA 2,642,084 G→A A254V (GCG→GTG)  rodZ ← MreB assembly cytoskeletal protein
RA 2,678,816 T→C E184E (GAA→GAG yphF ← putative sugar ABC transporter periplasmic binding protein
RA 2,682,797 T→C intergenic (‑52/‑66) yphG ← / → yphH DUF4380 domain‑containing TPR repeat protein/putative DNA‑binding transcriptional regulator
RA 2,706,632 T→C T165A (ACC→GCC)  lepA ← back‑translocating elongation factor EF4, GTPase
RA 2,707,777 C→T V9I (GTC→ATC)  rseC ← SoxR iron‑sulfur cluster reduction factor component
RA 2,734,543 (C)8→9 coding (492/732 nt) yfiH ← UPF0124 family protein
RA 2,735,653 A→G L120P (CTT→CCT)  rluD ← 23S rRNA pseudouridine(1911,1915,1917) synthase
RA 2,740,507 T→C Q215R (CAG→CGG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
RA 2,740,735 A→G L139P (CTG→CCG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
RA 2,747,358 T→C K146K (AAA→AAG ffh ← Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
RA 2,775,699 T→C pseudogene (155/180 nt) ypjI → pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
RA 2,807,897 A→G T256A (ACC→GCC)  proX → glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
RA 2,811,185 T→C L139P (CTC→CCC)  mprA → transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 2,813,385 A→G N257S (AAC→AGC)  emrB → multidrug efflux system protein
RA 2,817,743 T→C intergenic (‑240/+41) yqaB ← / ← argQ fructose‑1‑P and 6‑phosphogluconate phosphatase/tRNA‑Arg
RA 2,817,861 A→G intergenic (‑1/+198) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,819,577 A→G V812A (GTT→GCT)  alaS ← alanyl‑tRNA synthetase
RA 2,862,791 T→C Y153H (TAT→CAT)  ygbK → FliA‑regulated DUF1537 family protein
RA 2,862,827 C→T R165C (CGC→TGC)  ygbK → FliA‑regulated DUF1537 family protein
RA 2,873,099 T→C D80G (GAC→GGC)  ygbE ← DUF3561 family inner membrane protein
RA 2,899,237 T→C E61G (GAG→GGG)  ygcU ← putative FAD‑linked oxidoreductase
MC JC 2,911,491 Δ7,528 bp [relA][gudD] [relA], rlmD, barA, [gudD]
RA 2,923,308 T→C G159G (GGA→GGG truC ← tRNA(Ile1,Asp) pseudouridine(65) synthase
RA 2,942,711 A→G intergenic (‑144/+207) gcvA ← / ← ygdI glycine cleavage system transcriptional activator; autorepressor/DUF903 family verified lipoprotein
RA 2,955,448 T→C T186A (ACC→GCC)  recB ← exonuclease V (RecBCD complex), beta subunit
RA 2,986,699 T→C L285L (TTG→CTG)  yqeG → putative transporter
RA 2,989,983 A→G A126A (GCT→GCC yqeK ← uncharacterized protein
RA 2,994,461 (T)8→7 pseudogene (632/633 nt) ygeK ← pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator
RA 3,000,899 A→G E185E (GAA→GAG xdhA → xanthine dehydrogenase, molybdenum binding subunit
RA 3,012,391 T→C *311Q (TAA→CAA)  yqeA → putative amino acid kinase
RA 3,014,746 T→C E104E (GAA→GAG yqeC ← putative selenium‑dependent hydroxylase accessory protein
RA 3,016,166 A→G Y36C (TAC→TGC)  ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,019,115 T→C V1019A (GTC→GCC)  ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,039,247 A→G V166A (GTG→GCG)  xerD ← site‑specific tyrosine recombinase
RA 3,040,408 C→T intergenic (+32/+8) fldB → / ← cptA flavodoxin 2/toxin of CptAB toxin‑antitoxin pair
RA 3,049,805 (T)5→6 coding (863/1095 nt) gcvT ← aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
RA 3,083,334 T→C Y155C (TAT→TGT)  speB ← agmatinase
RA 3,108,362 G→A A265V (GCG→GTG)  speC ← ornithine decarboxylase, constitutive
RA 3,113,403 A→G C25C (TGT→TGC yghG ← secretin (GspDbeta) OM localization lipoprotein pilotin
RA 3,118,599 C→T V172I (GTT→ATT)  sslE ← putative secreted and surface‑associated lipoprotein mucinase
RA 3,130,088 T→G pseudogene (56/1101 nt) yghO ← pseudogene, IS‑interrupted
RA 3,213,331 T→C T95T (ACT→ACC rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,219,596 A→G N35D (AAC→GAC)  patA → putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
RA 3,227,104 G→A V291M (GTG→ATG)  ygjI → putative transporter
RA 3,236,954 G→A A139T (GCC→ACC)  ygjQ → DUF218 superfamily protein
RA 3,243,428 T→C E438E (GAA→GAG uxaC ← uronate isomerase
RA 3,246,757 C→T R36C (CGC→TGC)  exuR → hexuronate regulon transcriptional repressor; autorepressor
RA 3,257,613 G→A A133V (GCC→GTC)  yhaO ← putative transporter
RA 3,286,334 T→C L22P (CTT→CCT)  agaI → galactosamine‑6‑phosphate isomerase
RA 3,288,276 A→G N63D (AAT→GAT)  yraI → putative periplasmic pilin chaperone
RA 3,296,292 C→T R162C (CGC→TGC)  diaA → DnaA initiator‑associating factor for replication initiation
RA 3,304,806 G→C L338V (CTG→GTG)  mtr ← tryptophan transporter of high affinity
RA 3,311,736 G→A intergenic (‑52/+97) rpsO ← / ← truB 30S ribosomal subunit protein S15/tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
RA 3,320,094 T→C D507G (GAC→GGC)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 3,363,151 T→C G115G (GGT→GGC yhcD → putative outer membrane fimbrial subunit usher protein
RA 3,369,969 T→A T128S (ACC→TCC)  nanK ← N‑acetylmannosamine kinase
RA 3,376,341 +G coding (436/498 nt) sspB ← ClpXP protease specificity enhancing factor
RA 3,379,933 T→C D22G (GAC→GGC)  zapE ← divisome ATPase
RA 3,434,764 T→C L184S (TTG→TCG)  fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
RA 3,508,245 T→C T116A (ACG→GCG)  php ← phosphotriesterase homology protein
RA 3,554,511 T→C L476P (CTG→CCG)  malT → mal regulon transcriptional activator
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,636,796 C→A Q205H (CAG→CAT yhiN ← putative oxidoreductase
RA 3,687,764 T→C A470A (GCA→GCG bcsC ← cellulose synthase subunit
RA 3,699,320 T→C A369A (GCT→GCC bcsG → DUF3260 family cellulose production inner membrane protein
RA 3,785,668 C→T L137L (CTG→TTG)  gpmM → phosphoglycero mutase III, cofactor‑independent
RA 3,857,022 (C)5→4 coding (1383/1494 nt) yidJ ← sulfatase/phosphatase superfamily protein
RA 3,873,439 G→A D58D (GAC→GAT dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
RA 3,880,206 T→C D339G (GAC→GGC)  recF ← gap repair protein
RA 3,883,255 C→T D159N (GAT→AAT)  dnaA ← chromosomal replication initiator protein DnaA, DNA‑binding transcriptional dual regulator
RA 3,887,033 G→A G69D (GGC→GAC)  mnmE → tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
RA 3,913,871 T→C T444A (ACT→GCT)  glmU ← fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA 3,922,656 T→C intergenic (‑216/+401) atpI ← / ← rsmG ATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
RA 3,925,611 T→C D8G (GAC→GGC)  mnmG ← 5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
RA 3,930,513 T→C Q194R (CAG→CGG)  ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone
RA 3,951,228 A→G pseudogene (669/984 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 3,961,726 C→T R350R (CGC→CGT rep → DNA helicase and single‑stranded DNA‑dependent ATPase
RA 3,967,714 C→T intergenic (+38/‑202) rho → / → wecA transcription termination factor/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
RA 3,969,685 A→G I219V (ATC→GTC)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,970,062 C→T R344R (CGC→CGT wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,991,678 T→C S176P (TCC→CCC)  cyaA → adenylate cyclase
RA 3,998,071 G→T G30V (GGC→GTC)  uvrD → DNA‑dependent ATPase I and helicase II
RA 4,009,349 C→T C60C (TGC→TGT pldB → lysophospholipase L2
RA 4,086,550 T→C S179P (TCT→CCT)  fdhD → formate dehydrogenase formation protein
RA 4,128,316 A→G V27A (GTT→GCT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,182,890 A→G D549G (GAC→GGC)  rpoB → RNA polymerase, beta subunit
RA 4,184,036 T→C V931A (GTT→GCT)  rpoB → RNA polymerase, beta subunit
RA 4,184,514 C→T N1090N (AAC→AAT rpoB → RNA polymerase, beta subunit
RA 4,203,428 A→G S37G (AGC→GGC)  zraR → fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
RA 4,221,741 C→T R249R (CGG→CGA arpA ← ankyrin repeat protein
RA 4,234,063 T→C R102R (CGT→CGC pgi → glucosephosphate isomerase
RA 4,273,906 T→C intergenic (‑35/‑219) uvrA ← / → ssb ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC/single‑stranded DNA‑binding protein
RA 4,286,249 T→C T375A (ACC→GCC)  acs ← acetyl‑CoA synthetase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,316,378 A→G S281P (TCC→CCC)  phnM ← ribophosphonate triphosphate hydrolase
RA 4,358,354 A→G T88T (ACT→ACC cadA ← lysine decarboxylase, acid‑inducible
RA 4,379,942 A→G R192R (CGT→CGC frdB ← fumarate reductase (anaerobic), Fe‑S subunit
MC JC 4,398,054 Δ7 bp coding (643‑649/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,403,176 C→T P380L (CCG→CTG)  hflK → modulator for HflB protease specific for phage lambda cII repressor
RA 4,457,566 C→T A100A (GCG→GCA yjgA ← ribosome‑associated UPF0307 family protein
RA 4,485,806 Δ1 bp coding (146/1512 nt) pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,511,541 (G)5→6 coding (874/957 nt) fecD ← ferric citrate ABC transporter permease
RA 4,513,844 T→C A155A (GCA→GCG fecB ← ferric citrate ABC transporter periplasmic binding protein
RA 4,517,263 T→C Y152C (TAC→TGC)  fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,550,166 A→G T377T (ACT→ACC gntP ← fructuronate transporter
RA 4,554,471 T→C intergenic (+110/‑105) uxuB → / → uxuR D‑mannonate oxidoreductase, NAD‑dependent/fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
RA 4,586,594 (G)6→5 coding (168/3513 nt) hsdR ← endonuclease R Type I restriction enzyme
RA 4,606,094 (G)8→7 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,608,413 C→T R77C (CGT→TGT)  rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase
RA 4,622,019 C→T P84L (CCG→CTG)  yjjJ → putative protein kinase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423733–3424233 3424559–3424238 6–827 24 [23] [23] 24 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =13 (0.240)33 (0.680) 32/266 0.9 72.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 13 (0.270)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780513 (0.240)38 (0.780) 37/266 0.5 75.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 13 (0.270)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)35 (0.660) 31/290 1.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein