breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 998,193 IS5 (+) +4 bp coding (326‑329/540 nt) elfA → laminin‑binding fimbrin subunit
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
JC JC 1,347,480 IS5 (+) +4 bp coding (1430‑1433/1935 nt) rnb ← ribonuclease II
RA 1,931,977 T→G V366G (GTG→GGG)  purT → phosphoribosylglycinamide formyltransferase 2
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,794,550 T→G W100G (TGG→GGG)  gabP → gamma‑aminobutyrate transporter
MC JC 2,911,491 Δ7,528 bp [relA][gudD] [relA], rlmD, barA, [gudD]
RA 3,369,969 T→A T128S (ACC→TCC)  nanK ← N‑acetylmannosamine kinase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,128,380 C→A G6C (GGC→TGC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,182,583 C→T H447Y (CAC→TAC)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423743–3424234 3424561–3424238 5–819 22 [21] [21] 22 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3762331–3764074 3765204–3764079 6–2874 22 [21] [21] 23 rhsA Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =8 (0.160)25 (0.560) 23/266 1.6 79.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 6 (0.130)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078058 (0.160)27 (0.600) 25/266 1.4 80.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 6 (0.130)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related