Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 2
2 1 3
3 1 3
4 1 3
5 1 3
6 1 3
7 1 3
8 8 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 45 80
Multiple Base Substitutions 0 0
Deletions 17 40
Insertions 6 12
Mobile Element Insertions 6 10
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 74 142

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 8 3 77.5 2411342 99.2% 2392051 150.4
A1 F50 I2 R1 8 3 75.7 2348453 99.4% 2334362 150.4
A2 F50 I1 R1 15 3 106.9 3315624 99.1% 3285783 150.5
A2 F50 I2 R1 12 3 74.5 2311211 99.4% 2297343 150.4
A2 F50 I3 R1 15 4 77.6 2407323 99.3% 2390471 150.4
A3 F50 I1 R1 10 4 77.4 2402607 99.4% 2388191 150.4
A3 F50 I2 R1 10 3 74.4 2308986 99.3% 2292823 150.4
A3 F50 I3 R1 14 3 71.9 2231302 99.4% 2217914 150.4
A4 F50 I1 R1 13 3 61.7 1918205 99.2% 1902859 150.4
A4 F50 I2 R1 8 3 67.5 2095970 99.3% 2081298 150.4
A4 F50 I3 R1 7 3 94.6 2933770 99.5% 2919101 150.4
A5 F50 I1 R1 10 3 71.6 2231174 98.9% 2206631 150.4
A5 F50 I2 R1 15 5 101.6 3165943 99.0% 3134283 150.4
A5 F50 I3 R1 16 4 99.9 3128093 98.5% 3081171 150.4
A6 F50 I1 R1 9 3 83.8 2597395 97.9% 2542849 150.4
A6 F50 I2 R1 8 3 105.6 3339864 99.2% 3313145 150.4
A6 F50 I3 R1 9 4 107.1 3412234 99.5% 3395172 150.4
A7 F50 I1 R1 12 4 98.8 3067543 99.3% 3046070 150.4
A7 F50 I2 R1 14 3 114.6 3558734 99.4% 3537381 150.4
A7 F50 I3 R1 14 5 93.9 2910166 99.4% 2892705 150.4

Mutation Needle Plot

Mutation Targets

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