breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,807,248 +T coding (117/993 nt) proX → glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
RA 3,318,960 C→A A108A (GCC→GCA argG → argininosuccinate synthetase
RA 3,400,195 G→A A298V (GCA→GTA)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,823,811 G→A R471H (CGT→CAT)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423729–3424234 3424580–3424238 5–852 57 [56] [56] 60 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 683271 =113 (1.150)112 (1.140) 98/298 0.0 65.1% coding (123/1671 nt) hscC Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
?NC_000913 = 1907451 7 (0.070)coding (141/144 nt) yobF DUF2527 family heat‑induced protein
* ? NC_000913 1207790 =11 (0.110)67 (0.770) 57/266 1.0 83.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.190)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780511 (0.110)75 (0.860) 68/266 0.4 84.8% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.190)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1907448 =5 (0.050)119 (1.250) 83/290 0.1 96.0% coding (144/144 nt) yobF DUF2527 family heat‑induced protein
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region