breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC 83,679 (TAG)4→3 coding (28‑30/87 nt) leuL ← leu operon leader peptide
MC JC 257,908 Δ776 bp [crl] [crl]
RA 701,233 Δ1 bp coding (362/1221 nt) nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,025,435 T→A intergenic (+222/‑76) yodD → / → yedP uncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,807,248 +T coding (117/993 nt) proX → glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
RA 3,318,960 C→A A108A (GCC→GCA argG → argininosuccinate synthetase
RA 3,400,195 G→A A298V (GCA→GTA)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,473,612 A→C L157* (TTA→TGA)  rpsG ← 30S ribosomal subunit protein S7
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,823,811 G→A R471H (CGT→CAT)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423704–3424234 3424598–3424238 5–895 69 [67] [68] 69 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =21 (0.180)81 (0.800) 67/266 0.9 81.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.170)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780521 (0.180)102 (1.000) 80/266 0.3 85.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.170)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)125 (1.130) 98/290 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein