Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA5 ← / → uspC	IS1 family protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	33 (1.700)	21/384	NT	100%	intergenic (‑271/‑267)	insA5/uspC	IS1 family protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	17 (0.870)	13/384	NT	100%	intergenic (‑274/‑264)	insA5/uspC	IS1 family protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

CGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTT  >  NC_000913/2101540‑2101996                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        cGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGaa                                                                                                                                                                                                                                              >  2:59859/1‑221 (MQ=255)     tGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAg                                                                                                                                                                                                                                          <  1:59859/221‑1 (MQ=255)                            tCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGATTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCTTAAATGCCtt                                                                                                                                                             >  1:72396/1‑275 (MQ=255)                                    cagcccgGGTAGCCCTCCAGCACAGGTCAAAAACTTCACAGTGAATAAAGTAAACCTGGACGAAGCCATTTACACGGACAAAAGCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTAACAGAATAGGTACTGTCCTTAGGAATTTTTGTTTTATTAAGTAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATGAGc                                                                                                                                                     <  2:72396/275‑1 (MQ=25)                                                     aaGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCggg                                                                                                                                                                                                                                      >  1:247689/1‑177 (MQ=255)                                                         acaGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                                                                                                                                  <  2:247688/177‑1 (MQ=255)                                                                       tCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGATTTTTTGTTTTATTAAGGAAGGTGCGCATAAGCGGGGAAAGTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTa                                                                                                                  >  1:339847/1‑275 (MQ=255)                                                                       tCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGTAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                                                                                                                                   >  1:45017/1‑226 (MQ=255)                                                                            gTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCCGAATAGATACTTTCTTTCGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGAGGGGAAATTCTTATCGGCTGACTCAGTAATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCATAGAATCAGCCTATTAAGACCGTTTCTTCGCCTTTTAAGGCGTGTTcccc                                                                                                             >  1:115682/1‑275 (MQ=25)                                                                                       aaCCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGa                                                                                                                                                                                                                    >  1:52550/1‑161 (MQ=255)                                                                                       aaCCTTGATCGAAGCAATTTACACGCACAAAATCTGAAAAACGTTCCAGCATAAATGATCCTGCGCACCAACCAACCACCGTATCAGAATAGATACTTTCATTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTAATTTCCTTTCTTCATGTTTGAGCCGATTTTTTCTccag                                                                                                                                                                       >  2:54458/1‑204 (MQ=37)                                                                                         ccTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGGAGGTGCGAATAAGCGGGGAAAttcttc                                                                                                                                                                                                                            >  2:346038/1‑151 (MQ=255)                                                                                           ttGATCGAATCCAGTTACACGCACAAAATCTGAAAAACGTACCAGTAGAAATGGTTCTGCCCACCAATCAACAACCGGATCATAATAGATACTTTATTTAGTAATTTTTGTTTTATTAAGGAAGGTGCGAATAATCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                                                                                                                                   <  1:54458/206‑1 (MQ=25)                                                                                           ttGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                                                                                                                                <  2:52550/161‑1 (MQ=255)                                                                                             gATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGGAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                                                                                                                                        <  1:346039/151‑1 (MQ=255)                                                                                             gATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGtcatttcatt                                                                                                                                                                                                     >  1:395087/1‑170 (MQ=255)                                                                                              aTCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCAATGCCTTGAAt                                                                                                                                                         >  1:284993/1‑213 (MQ=255)                                                                                                 gAAGCCATTTAACCGCTCCAAAACTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTGGGTTTATTAAGGACGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCGGTCATTTCATttctt                                                                                                                                                                                                 <  2:395086/170‑1 (MQ=37)                                                                                                  aaGCCATTGAACCGAACAAAATCGGAAAAACGTACCAGCCTAAATGTGCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAATGTGCGAATAAGCGGGTAAATTCTGCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTTAGCCGATTTTTTCTCCCGTTAATGCCTTGAATCAGc                                                                                                                                                     <  2:284992/213‑1 (MQ=25)                                                                                                                       ccaaaaaaaCTTACCAGCCGAAAGTATCCTAGGAACCAATCAACAACCGTAGCAGAATAGGTACTTTCTTTACGAATTTTTGTGTTATTAAGGAAGGTGCGAATGAGGGCGGAAATTCTGCTCTGCTGACCCAGTCATTTCATTTCTGCATGTTTTAGCCGATTTTTTCTCCCGTAAATGCCTCGAATCAGCCTAGTCATACCGTGTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGGCCg                                                                  <  2:339846/271‑1 (MQ=17)                                                                                                                           aaaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACt                                                                                                                                                                                                                  >  1:265006/1‑127 (MQ=255)                                                                                                                               aCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTGTCTTTAGGAATTTTTGTTTGATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGt                                                                                                                                                                                                              <  2:265005/127‑1 (MQ=255)                                                                                                                                                  ccTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACt                                                                                                                                                                                                                  >  1:153274/1‑104 (MQ=255)                                                                                                                                                      cgcACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGt                                                                                                                                                                                                              <  2:153273/104‑1 (MQ=255)                                                                                                                                                          ccAATCAACAACCCTATCAGAATAGATACTTTCTTTAGGAATTTTAGTTTTATTAAGGGAGGTGCAAATAAGCGGGGAAAGTCTTCGCGGCTAACTCAGTCATTTCATTTCTTCATGTTAGAGCCGATTTTTTCTCCAGTAAATGCCTTGAATCAGc                                                                                                                                                     <  1:372955/157‑1 (MQ=18)                                                                                                                                                             aTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATCAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtt                                                                                                                                                                                 >  2:186183/1‑126 (MQ=255)                                                                                                                                                             aTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTg                                                                                   >  1:67986/1‑220 (MQ=255)                                                                                                                                                                 acaacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                                                                                                             <  1:186184/126‑1 (MQ=255)                                                                                                                                                                 acaacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGt                                                                               <  2:67986/220‑1 (MQ=255)                                                                                                                                                                     ccGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGAGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcatt                                                                                                                                                                                                     >  2:39356/1‑98 (MQ=255)                                                                                                                                                                        tATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTAATAAGGACGGTGCGAATAAGCGGGGGAATTCTTTTCGGCTGACTCAGGTAATTCATTTCTTCATGTGTGAGCGGATATTTTCTCCCTTAAATGCCTTGAATCAGTCTATGTAGACCGTTTGTTCGCCATTTAAGGCTTTATCCCCAGtttt                                                                                                       >  2:82154/1‑189 (MQ=255)                                                                                                                                                                         aTCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                                                                                                                                 <  1:39356/98‑1 (MQ=255)                                                                                                                                                                         aTCAGAATAGATACTTGCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGATATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                                                                                                                     <  1:1590/142‑1 (MQ=255)                                                                                                                                                                            aGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTGCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTGCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                                                   <  1:82154/189‑1 (MQ=255)                                                                                                                                                                            aGAATAGATACTTTCTTTAGGAATGTTTGTCGTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCTCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                                                   <  1:394903/189‑1 (MQ=255)                                                                                                                                                                                tAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                                                                                                                          >  2:53245/1‑130 (MQ=255)                                                                                                                                                                                    tACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                                                                                                                                      <  1:53245/130‑1 (MQ=255)                                                                                                                                                                                        ttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                                                                                                                                   >  2:46480/1‑113 (MQ=255)                                                                                                                                                                                        ttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGtttt                                                                                                       >  2:309059/1‑173 (MQ=255)                                                                                                                                                                                        ttctttAGGAATTTTTGTTTTATCAAGGAAGCTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTGCGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCTTTTAAGGCTTTCTCCCCAGCTCTTCGTGAGATCTCGCCCCCTGACGTCCTATTTGGTCCGCCCGAAACAGGTTGGCTAGACTGAATAACATCGCCAGTTGGTTAACGTTTTTCATCACccccct  >  2:293315/1‑272 (MQ=11)                                                                                                                                                                                        ttctttAGGAATTTTTGTTTTACTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGc                                                  >  2:221124/1‑226 (MQ=255)                                                                                                                                                                                            ttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGcc                                                                                                                                                               <  1:46480/113‑1 (MQ=255)                                                                                                                                                                                            ttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                                                   <  1:309060/173‑1 (MQ=255)                                                                                                                                                                                            ttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGc                                              <  1:221125/226‑1 (MQ=39)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                        CGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACCCCTT  >  NC_000913/2101540‑2101996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.