Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA5 ← / → uspC	IS1 family protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	33 (1.700)	21/384	NT	100%	intergenic (‑271/‑267)	insA5/uspC	IS1 family protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	17 (0.870)	13/384	NT	100%	intergenic (‑274/‑264)	insA5/uspC	IS1 family protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCA‑GTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTT  >  NC_000913/2289843‑2290317                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                              tgctgcATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATAACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTATGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGATGATGACTCATGATGCACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATaa                                                                                                                                                                                                           >  1:249909/1‑275 (MQ=14) tgctgcATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCCCGATGACTTCCACCATGTTTTGCCATGTCAGAATCTGCCCCATGCGGGACAAGAAAATCTATTCTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGACGGTACGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGACATCATATCAGGGACTTGTTCGCACCTTCCATaa                                                                                                                                                                                                           >  1:263417/1‑275 (MQ=255)                                                ccGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGCCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAATTTGACGACTCATGATGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGACTTTTTCGCACCTACAATAACGCTTTAGCAACCAGAACAGAAATTGCGGAACGACAAAGAAAAAAAg                                                                                                                                                            >  1:188675/1‑275 (MQ=14)                                                                        aaaCGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGGGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACACTGTTCTATGGCTCCAGATGACAAACATGATCTCATATAAGGGACTTGTTCGCACCTTCCATAACGCTg                                                                                                                                                                                                      >  1:231574/1‑209 (MQ=18)                                                                                                                     cTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTTTTCGCACCTTCCATAACGCTGTCGCGACCAGAACAGATATTGCGGAACGACACAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTACGAAAATAATTTCTTTTAAAAACaatttggttacgaa                                                                                        >  2:250355/1‑261 (MQ=18)                                                                                                                     cTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGAAAAACATGATCTCATATCATGGCCTCGTCAGCACCTTCCATAAAGc                                                                                                                                                                                                        >  1:368862/1‑162 (MQ=11)                                                                                                                         aTGCGGGACAAGAAAATCGCTTTTCTGGTCTGACGGCGGTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACg                                                                                                                                                                                                         <  1:26346/157‑1 (MQ=18)                                                                                                                         aTGCGGGACAAGAAAATCGCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCTGCGAAGGGAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTACAGATGACAAACATTATCTCATATCAGGGACTTGTTCGCACCGTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                                                                                   <  1:250356/259‑1 (MQ=255)                                                                                                                          tGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACa                                                                                                                                                             >  1:270890/1‑200 (MQ=255)                                                                                                                              ggACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAAc                                                                                                                                                         <  2:270889/200‑1 (MQ=255)                                                                                                                                         tctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGc                                                                                                                                                                                                        >  1:140838/1‑142 (MQ=34)                                                                                                                                            ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCATAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                                                                                           <  2:199978/200‑1 (MQ=255)                                                                                                                                            ctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                                                                                           >  1:199979/1‑200 (MQ=255)                                                                                                                                             ttttCTGGTCTGACTGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                                                                                    <  2:140837/142‑1 (MQ=38)                                                                                                                                                                              aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAc                                                                                    >  2:176474/1‑221 (MQ=255)                                                                                                                                                                                tGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTg                                                                                                              >  2:361003/1‑193 (MQ=255)                                                                                                                                                                                  tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCAt                                                                                <  1:176475/221‑1 (MQ=255)                                                                                                                                                                                    ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaa                                                                                                          <  1:361004/193‑1 (MQ=255)                                                                                                                                                                                     gCGACGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGCCTTGTACGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGACAGACCCAGAGAAACAGAACCAGATCGATGCATTGAGCTTTCCAACCATGAAATTAATTGCTTTTAAAATAATTGGGTACAGACAATACCCCTAGCTCACAAACCGGGGTCAGGTTTTTAAAAAATTTTTAaaaaaaagaccacgatttt                       >  2:3695/1‑262 (MQ=21)                                                                                                                                                                                                     gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaatta                                                                                                                     >  1:186134/1‑165 (MQ=255)                                                                                                                                                                                                         cATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTg                                                                                                                 <  2:186133/165‑1 (MQ=255)                                                                                                                                                                                                          atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATTATCTCATATCACTGACTTGTTCGCACCGTCCATAACGCTGTAGCTATCAGAACAGATATTGCGGAACGACAAGGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTATTTGCTg                                                                                                              >  1:247431/1‑167 (MQ=37)                                                                                                                                                                                                                  cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGCTATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCAATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCA‑Gttttt                                                      >  1:34689/1‑214 (MQ=255)                                                                                                                                                                                                                      gTTCTATGGCTCCAGATGACAAACATGATCTCATATCAAGGACTTTTGCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATGGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAAGTAATTGCTGTTAAAAGCAGTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCA‑GTTTTTaaaa                                                  <  2:34689/214‑1 (MQ=255)                                                                                                                                                                                                                                                                      cGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTCAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTTGGTACAGACAATACCCATAGCTCCATACCCGGAGTTA‑GTTTTTAAAAACCTGTTAAAGAAATGCACAAGTAATGTTATTGATTTTTTAGTTGttt  >  1:212988/1‑214 (MQ=255)                                                                                                                                                                                                                                                                             tCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaa                                                                                                          >  1:14506/1‑104 (MQ=255)                                                                                                                                                                                                                                                                             tCCATAACGCTGTAACCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATcc                                                                                                                               >  2:267479/1‑83 (MQ=255)                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                              TGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCA‑GTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTT  >  NC_000913/2289843‑2290317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.