breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
RA 859,587 G→A A164T (GCA→ACA)  fsa → fructose‑6‑phosphate aldolase
MC JC 1,613,500 Δ4 bp coding (124‑127/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 190955 190997 43 3 [1] [1] 2 ispC 1‑deoxy‑D‑xylulose‑5‑phosphate reductoisomerase
* * ÷ NZ_CP009273 356981 357567 587 3 [0] [0] 2 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 434598 434645 48 2 [1] [1] 2 dxs 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
* * ÷ NZ_CP009273 772202 772283 82 2 [1] [1] 2 tolA cell envelope integrity protein TolA
* * ÷ NZ_CP009273 849325 849354 30 2 [1] [1] 2 ybiR anion transporter
* * ÷ NZ_CP009273 2616965 2616970 6 2 [0] [1] 3 ppk1 polyphosphate kinase 1
* * ÷ NZ_CP009273 3132288 3132298 11 2 [1] [0] 6 yghU glutathione‑dependent disulfide‑bond oxidoreductase
* * ÷ NZ_CP009273 3569081 3569146 66 2 [1] [1] 2 gntU gluconate transporter
* * ÷ NZ_CP009273 3626675 3637014 10340 8 [0] [1] 2 [BW25113_RS18065]–[prlC] [BW25113_RS18065],yhiM,yhiN,pitA,uspB,uspA,BW25113_RS26225,BW25113_RS26230,dtpB,rsmJ,[prlC]
* * ÷ NZ_CP009273 3637103 3646518–3645391 8289–9416 2 [1] [1] 2 [prlC]–[BW25113_RS18160] [prlC],rlmJ,gorA,dinQ,arsR,arsB,arsC,BW25113_RS18155,[BW25113_RS18160]
* * ÷ NZ_CP009273 3682703 3682741 39 3 [1] [1] 2 bcsZ cellulose synthase complex periplasmic endoglucanase BcsZ
* * ÷ NZ_CP009273 3708051 3708071 21 3 [1] [1] 3 bisC biotin sulfoxide reductase
* * ÷ NZ_CP009273 3732688 3732691 4 2 [1] [0] 2 malS alpha‑amylase
* * ÷ NZ_CP009273 4049872 4049874 3 2 [0] [0] 3 glnL/glnA nitrogen regulation protein NR(II)/glutamate‑‑ammonia ligase
* * ÷ NZ_CP009273 4113111 4113112 2 2 [0] [1] 2 ftsN cell division protein FtsN

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)59 (4.060) 34/170 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 1391494NA (NA)9 (0.630) 7/168 0.7 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)21 (1.440) 14/170 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 = 20007839 (0.620)3 (0.230) 3/154 1.3 24.8% coding (1147/1488 nt) amyA alpha‑amylase
?NZ_CP009273 = 2000836 10 (0.760)coding (1200/1488 nt) amyA alpha‑amylase
* ? NZ_CP009273 = 36266740 (0.000)8 (0.550) 8/170 0.6 100% pseudogene (1145/1607 nt) BW25113_RS18065 YhiJ/YhiL family protein
?NZ_CP009273 3645396 = NA (NA)pseudogene (746/747 nt) BW25113_RS18155 hypothetical protein