breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 49,765 | C→T | intergenic (+134/‑58) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
RA | 49,884 | C→T | P21L (CCG→CTG) | folA → | type 3 dihydrofolate reductase |
RA | 214,743 | A→C | Y174D (TAC→GAC) | proS ← | proline‑‑tRNA ligase |
MC JC | 2,399,465 | Δ7 bp | coding (650‑656/939 nt) | lrhA ← | transcriptional regulator LrhA |
RA | 3,754,231 | G→T | R127S (CGC→AGC) | selA ← | L‑seryl‑tRNA(Sec) selenium transferase |
RA | 4,267,454 | C→T | R20C (CGC→TGC) | soxR → | redox‑sensitive transcriptional activator SoxR |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NZ_CP009273 | 356979 | 357570 | 592 | 6 [5] | [5] 7 | lacA | galactoside O‑acetyltransferase |
* | * | ÷ | NZ_CP009273 | 982669 | 982751 | 83 | 6 [5] | [4] 6 | ompF/asnS | porin OmpF/asparagine‑‑tRNA ligase |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP009273 | 360104 = | NA (NA) | 176 (3.780) | 47/168 | 0.0 | NA | intergenic (‑35/+251) | lacZ/BW25113_RS23320 | beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein |
? | NZ_CP009273 | = 360286 | NA (NA) | intergenic (‑217/+69) | lacZ/BW25113_RS23320 | beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein | |||||
* | ? | NZ_CP009273 | 454243 = | 39 (0.840) | 12 (0.270) | 11/162 | 1.5 | 24.2% | intergenic (+87/‑101) | clpX/lon | ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La |
? | NZ_CP009273 | 603467 = | NA (NA) | intergenic (+23/‑54) | hokE/BW25113_RS03025 | type I toxin‑antitoxin system toxin HokE/IS4‑like element IS421 family transposase | |||||
* | ? | NZ_CP009273 | = 454255 | 45 (0.970) | 13 (0.290) | 9/160 | 1.8 | 23.3% | intergenic (+99/‑89) | clpX/lon | ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La |
? | NZ_CP009273 | = 604803 | NA (NA) | intergenic (+170/+112) | BW25113_RS03025/entD | IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD | |||||
* | ? | NZ_CP009273 | 1203246 = | 11 (0.240) | 14 (0.370) | 13/136 | 0.9 | 66.9% | coding (290/630 nt) | BW25113_RS05990 | hypothetical protein |
? | NZ_CP009273 | 1205075 = | 5 (0.130) | coding (40/540 nt) | BW25113_RS06005 | phage tail protein | |||||
* | ? | NZ_CP009273 | = 1203261 | 11 (0.240) | 26 (0.690) | 16/136 | 0.6 | 78.9% | coding (305/630 nt) | BW25113_RS05990 | hypothetical protein |
? | NZ_CP009273 | = 1205058 | 5 (0.130) | coding (57/540 nt) | BW25113_RS06005 | phage tail protein | |||||
* | ? | NZ_CP009273 | = 1391494 | NA (NA) | 31 (0.670) | 18/166 | 0.8 | 100% | intergenic (+145/‑128) | BW25113_RS06980/ynaJ | IS5‑like element IS5 family transposase/DUF2534 family protein |
? | NZ_CP009273 | 1972747 = | 0 (0.000) | intergenic (‑293/‑487) | flhD/uspC | flagellar transcriptional regulator FlhD/universal stress protein UspC | |||||
* | ? | NZ_CP009273 | = 1972751 | 0 (0.000) | 57 (1.220) | 33/168 | 0.2 | 100% | intergenic (‑297/‑483) | flhD/uspC | flagellar transcriptional regulator FlhD/universal stress protein UspC |
? | NZ_CP009273 | = 2283592 | NA (NA) | intergenic (‑68/‑387) | BW25113_RS11435/narP | IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP |