breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,884 C→T P21L (CCG→CTG)  folA → type 3 dihydrofolate reductase
RA 214,743 A→C Y174D (TAC→GAC)  proS ← proline‑‑tRNA ligase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,754,231 G→T R127S (CGC→AGC)  selA ← L‑seryl‑tRNA(Sec) selenium transferase
RA 4,267,454 C→T R20C (CGC→TGC)  soxR → redox‑sensitive transcriptional activator SoxR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356979 357570 592 6 [5] [5] 7 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 982669 982751 83 6 [5] [4] 6 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)176 (3.780) 47/168 0.0 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 454243 =39 (0.840)12 (0.270) 11/162 1.5 24.2% intergenic (+87/‑101) clpX/lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
?NZ_CP009273 603467 = NA (NA)intergenic (+23/‑54) hokE/BW25113_RS03025 type I toxin‑antitoxin system toxin HokE/IS4‑like element IS421 family transposase
* ? NZ_CP009273 = 45425545 (0.970)13 (0.290) 9/160 1.8 23.3% intergenic (+99/‑89) clpX/lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
?NZ_CP009273 = 604803 NA (NA)intergenic (+170/+112) BW25113_RS03025/entD IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD
* ? NZ_CP009273 1203246 =11 (0.240)14 (0.370) 13/136 0.9 66.9% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 5 (0.130)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326111 (0.240)26 (0.690) 16/136 0.6 78.9% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 5 (0.130)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)31 (0.670) 18/166 0.8 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)57 (1.220) 33/168 0.2 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP