breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 91 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,594,1610CT68.9% 10.7 / 37.6 45S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092734,594,1670CT64.4% ‑0.8 / 39.1 45S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273822,9550TG60.7% 19.9 / 30.8 28T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP009273585,3900TG60.0% ‑6.5 / 14.4 15T341P (ACC→CCC) nfrAbacteriophage adsorption protein NfrA
*NZ_CP0092731,673,5610AC60.0% 10.2 / 15.5 20Y293S (TAC→TCC) ydgHDUF1471 family protein YdgH
*NZ_CP0092732,738,4480AC60.0% 3.6 / 21.4 26V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
*NZ_CP009273595,0320TG57.1% ‑4.9 / 23.4 21V288G (GTG→GGG) cusACu(+)/Ag(+) efflux RND transporter permease subunit CusA
*NZ_CP0092732,621,9840AC57.1% 15.8 / 25.1 21V105G (GTC→GGC) pdeFcyclic‑guanylate‑specific phosphodiesterase PdeF
*NZ_CP0092734,186,0090TG57.1% 11.5 / 16.4 14T7P (ACC→CCC) thiCphosphomethylpyrimidine synthase ThiC
*NZ_CP0092732,334,2240TA56.0% 4.3 / 26.4 25D1142V (GAT→GTT) yfaLAIDA‑I family autotransporter adhesin YfaL/EhaC
*NZ_CP0092732,336,7850AC55.2% 31.5 / 40.4 29G288G (GGT→GGGyfaLAIDA‑I family autotransporter adhesin YfaL/EhaC
*NZ_CP0092734,561,0000AC55.2% 27.3 / 38.2 30V131G (GTG→GGG) yjiRPLP‑dependent aminotransferase family protein
*NZ_CP009273822,9630TG54.8% 16.6 / 43.0 31D492A (GAC→GCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP00927316,7340AC54.5% 19.7 / 28.8 22intergenic (+177/+17)BW25113_RS00080/mokCIS4‑like element IS421 family transposase/type I toxin‑antitoxin system toxin MokC
*NZ_CP009273353,6010CA54.5% 14.9 / 38.5 33L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP0092731,213,8700AC54.2% 23.5 / 30.1 24T363P (ACC→CCC) pdeGcyclic diguanylate phosphodiesterase
*NZ_CP0092731,819,3180GA54.2% 7.3 / 30.9 24intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy
*NZ_CP0092733,273,8300AC54.2% 21.2 / 29.6 24T85T (ACA→ACCagaVPTS N‑acetylgalactosamine transporter subunit IIB
*NZ_CP0092733,924,0780TG54.2% 19.6 / 28.8 24T126P (ACC→CCC) ravAATPase RavA
*NZ_CP009273276,8000AC53.8% 67.8 / 79.4 52Y299D (TAT→GAT) yagAIS481‑like element ISEc19 family transposase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 3499137 =45 (1.220)3 (0.090) 3/154 3.0 6.8% coding (243/1086 nt) yhfS aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
?NZ_CP009273 3499152 = 41 (1.210)coding (228/1086 nt) yhfS aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme