New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	4398991 =	52 (2.350)	3 (0.170)	3/140	1.6	7.3%	intergenic (+79/‑101)	rnr/rlmB	ribonuclease R/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
	?	NZ_CP009273	4399052 =	34 (1.870)					intergenic (+140/‑40)	rnr/rlmB	ribonuclease R/23S rRNA (guanosine(2251)‑2'‑O)‑methyltransferase RlmB
Rejected: Frequency below/above cutoff threshold.

TACTCGTTGTTTACGTTTTGCCTGATGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGGATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP009273/4399086‑4398991 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atgtaggccggataaACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCT  >  NZ_CP009273/4399052‑4399135                                                                                                                                                                                      TACTCGTTGTTTACGTTTTGCCTGATGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCC                                                                                            <  1:429332/90‑1   CTCGTTGTTTACGTTTTGCCTGATGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGG                                                                                          >  1:292751/1‑90     CGTTGTTTACGTTTTGCCTGATGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGGAT                                                                                        >  1:489871/1‑90        TGTTTACGTTTTGCCTGATGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGGATAAA                                                                                     <  1:127287/90‑1                          TGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGGATAAACGTAGTGCATCAGGCAAA                                                                   <  2:192270/90‑1                                      ATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACG                                                       <  1:188637/90‑1                                                                                   TGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAG          <  1:138021/90‑1                                                                                   TGTAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAG          <  1:623170/90‑1                                                                                     TAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGC        <  2:604016/90‑1                                                                                     TAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGC        <  2:348920/90‑1                                                                                     TAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGC        <  2:243396/90‑1                                                                                     TAGGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGC        <  2:119248/90‑1                                                                                     TAGGCCGGATAAACGGAGTGCATCAGGCAAACCGTAAACAACGACTACATTAATGAGCGAAATGATTTACGGCATCCACGAAGTGCAGGC        <  2:107490/90‑1                                                                                       GGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCGGTGCAGGCCC      <  1:114822/90‑1                                                                                       GGCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCC      >  1:229450/1‑90                                                                                        GCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCT     >  1:599139/1‑90                                                                                        GCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCT     >  1:593689/1‑90                                                                                        GCCGGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCT     >  1:185006/1‑90                                                                                           GGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCT  >  1:207334/1‑90                                                                                           GGATAAACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCT  >  2:549534/1‑90                                                                                                                                                                                      TACTCGTTGTTTACGTTTTGCCTGATGCACTACGTTTATCCGGCCTACATGATCTCTGCAATATATTGAATTTGCGTGATTATGTAGGCCGGATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP009273/4399086‑4398991 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atgtaggccggataaACGTAGTGCATCAGGCAAAACGTAAACAACGAGTACATTAATGAGCGAAATGATTTACGGCATCCACGCAGTGCAGGCCCTGCT  >  NZ_CP009273/4399052‑4399135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.