New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 254128217 (0.820)3 (0.150) 3/168 1.9 16.8% coding (1251/1425 nt) murP PTS N‑acetylmuramic acid transporter subunit IIBC
?NZ_CP009273 2541287 = 13 (0.630)coding (1256/1425 nt) murP PTS N‑acetylmuramic acid transporter subunit IIBC
Rejected: Frequency below/above cutoff threshold.

GCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/2541195‑2541282
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCA  >  NZ_CP009273/2541287‑2541352
                                                                                                                                                          
GCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTTGTGGGGTCTGCCGGt                                                                  >  1:399385/1‑89
                                    CGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCG                              >  1:237948/1‑90
                                                                CTGATAGCCTGGTGGGGTCTGCCGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCA  <  2:237948/90‑1
                                                                                                                                                          
GCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/2541195‑2541282
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCA  >  NZ_CP009273/2541287‑2541352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.