New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2887966 =45 (1.360)4 (0.130) 4/246 3.5 8.3% coding (347/735 nt) cysH phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
?NC_000913 2888001 = 45 (1.430)coding (312/735 nt) cysH phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGTTTGCGCATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTT  >  NC_000913/2887823‑2888113
                                                                                                                                               |                                                                                                                                                   
gACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGTTTGCGCATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCt                                                                                                                                                >  8:105715/1‑149 (MQ=255)
                           cAGCAAACCAGGTTTGCGCATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCt                                                                                                                                            <  4:49649/126‑1 (MQ=255)
                           cAGCAAACCAGGTTTGCGCATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCt                                                                                                                                            >  3:49649/1‑126 (MQ=255)
                                aaCCAGGTTTGCGCATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTa                                                                                                                >  2:7675/1‑149 (MQ=255)
                                            gcATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCttt                                                                                                                             >  4:16323/1‑124 (MQ=255)
                                            gcATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCttt                                                                                                                             <  3:16323/124‑1 (MQ=255)
                                            gcATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGtt                                                                                                    >  8:137832/1‑149 (MQ=255)
                                            gcATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGtt                                                                                                    <  7:137832/149‑1 (MQ=255)
                                             cATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTgc                                                                                                                                  >  6:79972/1‑118 (MQ=255)
                                             cATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTgc                                                                                                                                  <  5:79972/118‑1 (MQ=255)
                                              aTTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGa                                                                                                  <  7:105715/149‑1 (MQ=255)
                                                  aGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCtt                                                                                              <  1:7675/149‑1 (MQ=255)
                                                    ttCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCtt                                                                                              >  6:89582/1‑147 (MQ=255)
                                                    ttCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCtt                                                                                              >  6:91786/1‑147 (MQ=255)
                                                    ttCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGa                                                                                            <  5:91786/149‑1 (MQ=255)
                                                    ttCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGa                                                                                            <  5:89582/149‑1 (MQ=255)
                                                           agagCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGtt                                                                                                    >  4:75190/1‑134 (MQ=255)
                                                           agagCCCGGGTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGtt                                                                                                    <  3:75190/134‑1 (MQ=255)
                                                                       aTCGGTTCGAGTTTGTTGATGTCTTTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTcc                                                                                    <  2:38531/138‑1 (MQ=255)
                                                                       aTCGGATCGACTTTGTTGATGTCATTGTACTTGTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGATGAGCTTGAGTTTGTcc                                                                                    >  1:38531/1‑138 (MQ=255)
                                                                           gTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAAt                                                                      >  6:139814/1‑148 (MQ=255)
                                                                                 cTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCgg                                                               <  4:81570/149‑1 (MQ=255)
                                                                                  tttGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCgg                                                               >  8:134043/1‑148 (MQ=255)
                                                                                  tttGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCgg                                                               >  7:15588/1‑148 (MQ=255)
                                                                                     gttgATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAg                                                            <  7:134043/148‑1 (MQ=255)
                                                                                            tCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCgg                                                    >  5:6939/1‑149 (MQ=255)
                                                                                             cATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCgg                                                    >  4:45199/1‑148 (MQ=255)
                                                                                                          aaTGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGt                                                          <  8:89829/129‑1 (MQ=255)
                                                                                                          aaTGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGt                                                          >  7:89829/1‑129 (MQ=255)
                                                                                                            tGCCTTCAACGCCGTGCTCCCAGAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCaa                                                                       <  7:24605/114‑1 (MQ=255)
                                                                                                            tGCCTTCAACGACATGCTCCCACAGATATCCGTAGCGTGCTTCCAGCCAGGCTGCGCTATCGATAGCACGGTACACTTTCACTTTGAGCTAGAGTTATTAAGTTAAATAGTCaa                                                                       >  8:24605/1‑114 (MQ=255)
                                                                                                                ttCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCgg                                >  8:84013/1‑149 (MQ=255)
                                                                                                                  cAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGt                               <  6:6939/148‑1 (MQ=255)
                                                                                                                   aaCGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGa                             <  3:45199/149‑1 (MQ=255)
                                                                                                                   aaCGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGa                             <  8:15588/149‑1 (MQ=255)
                                                                                                                       cccTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGAt                         <  7:84013/149‑1 (MQ=255)
                                                                                                                        ccTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAAc                                                >  2:139257/1‑125 (MQ=255)
                                                                                                                        ccTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAAc                                                <  1:139257/125‑1 (MQ=255)
                                                                                                                               ccACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGaa                 >  7:63913/1‑149 (MQ=255)
                                                                                                                                cacaGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGa                             <  2:56916/136‑1 (MQ=255)
                                                                                                                                cacaGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGa                             >  1:56916/1‑136 (MQ=255)
                                                                                                                                    gTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATcc            <  5:139814/149‑1 (MQ=255)
                                                                                                                                            tAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCAc                      >  2:117559/1‑131 (MQ=255)
                                                                                                                                            tAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCAc                      <  1:117559/131‑1 (MQ=255)
                                                                                                                                              gCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAAttt  >  5:27353/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGTTTGCGCATTCAGTTCTTTCAGAGCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTT  >  NC_000913/2887823‑2888113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.