Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AM260479	1,714,344	(CGAAGC)17→9	coding (1338‑1385/3312 nt)	h16_A1582 →	DNA segregation ATPase FtsK/SpoIIIE related protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AM260479	1714287	1714334	48	12 [0]	[0] 12	h16_A1582	DNA segregation ATPase FtsK/SpoIIIE related protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	1714335 =	0 (0.000)	12 (1.010)	11/94	0.2	100%	coding (1329/3312 nt)	h16_A1582	DNA segregation ATPase FtsK/SpoIIIE related protein
	?	AM260479	= 1714286	0 (0.000)					coding (1280/3312 nt)	h16_A1582	DNA segregation ATPase FtsK/SpoIIIE related protein

GGACATTGCCGGCGACGCAGAACCGCAAGCACTGGCTACCGTCACCCTGCCAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGAT  >  AM260479/1714236‑1714404                                                   |                                                                                                                       gggcattGCCGGCGACGCAGAACCGCAAGCACTGGCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtca                                     <  1:63383‑M2/131‑84 (MQ=255)           ggCGACGCAGAACCGCAAGCACTGGCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacc                           <  1:28402‑M2/134‑94 (MQ=255)                 gCAGAACCGCAAGCACTGGCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacccgaagc                     <  1:484685‑M2/134‑100 (MQ=255)                       ccGCAAGCACTGGCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaa                             >  1:445021‑M2/1‑29 (MQ=255)                       ccGCAAGCACTGGCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaa                             <  2:445021‑M2/120‑92 (MQ=255)                              cACTGGCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacccgaagcgctggcagcagcc        >  2:28402‑M2/1‑22 (MQ=255)                                   gCTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacccgaagcgctggcagcagccgtgcc   <  2:1092195‑M2/134‑118 (MQ=255)                                    cTACCGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacccgaagcgctggcagcagccgtgccc  >  1:1092195‑M2/1‑16 (MQ=255)                                       ccGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacccgaagcgctggcagcagccgtgcc   >  1:471622‑M2/1‑13 (MQ=255)                                       ccGTCACCCTGCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggagatcacgccgttgtcattggggaacccgaagcgctggcagcagccgtgcc   <  2:471622‑M2/130‑118 (MQ=255)                                                 gCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggag                                                    >  1:789401‑M2/1‑3 (MQ=255)                                                 gCCagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagccgaagcgaggagcggag                                                    <  2:789401‑M2/71‑69 (MQ=255)                                                   |                                                                                                                       GGACATTGCCGGCGACGCAGAACCGCAAGCACTGGCTACCGTCACCCTGCCAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGAT  >  AM260479/1714236‑1714404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑