Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AM260479	1,714,344	(CGAAGC)17→9	coding (1338‑1385/3312 nt)	h16_A1582 →	DNA segregation ATPase FtsK/SpoIIIE related protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AM260479	1714287	1714334	48	12 [0]	[0] 12	h16_A1582	DNA segregation ATPase FtsK/SpoIIIE related protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	1714335 =	0 (0.000)	12 (1.010)	11/94	0.2	100%	coding (1329/3312 nt)	h16_A1582	DNA segregation ATPase FtsK/SpoIIIE related protein
	?	AM260479	= 1714286	0 (0.000)					coding (1280/3312 nt)	h16_A1582	DNA segregation ATPase FtsK/SpoIIIE related protein

GCCAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGCCGTGCCC  >  AM260479/1714284‑1714452                                                    |                                                                                                                      gggcattgccggcgacgcagaaccgcaagcactggctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCa                                     <  1:63383‑M1/83‑1 (MQ=255)           ggcgacgcagaaccgcaagcactggctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAAcc                           <  1:28402‑M1/93‑1 (MQ=255)                 gcagaaccgcaagcactggctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAgc                     <  1:484685‑M1/99‑1 (MQ=255)                       ccgcaagcactggctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGaa                             >  1:445021‑M1/30‑120 (MQ=255)                       ccgcaagcactggctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGaa                             <  2:445021‑M1/91‑1 (MQ=255)                              cactggctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGcc        >  2:28402‑M1/23‑134 (MQ=255)                                   gctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGCCGTGcc   <  2:1092195‑M1/117‑1 (MQ=255)                                    ctaccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGCCGTGccc  >  1:1092195‑M1/17‑134 (MQ=255)                                       ccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGCCGTGcc   >  1:471622‑M1/14‑130 (MQ=255)                                       ccgtcaccctgccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGCCGTGcc   <  2:471622‑M1/117‑1 (MQ=255)                                                 gccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGgag                                                    >  1:789401‑M1/4‑71 (MQ=255)                                                 gccAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGgag                                                    <  2:789401‑M1/68‑1 (MQ=255)                                                    |                                                                                                                      GCCAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCCGAAGCGAGGAGCGGAGATCACGCCGTTGTCATTGGGGAACCCGAAGCGCTGGCAGCAGCCGTGCCC  >  AM260479/1714284‑1714452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑