New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	= 3384911	31 (1.160)	4 (0.160)	4/200	2.0	12.5%	coding (409/1086 nt)	trmU	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑methyltransferase
	?	AM260479	= 3384940	27 (1.080)					coding (380/1086 nt)	trmU	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑methyltransferase
Rejected: Frequency below/above cutoff threshold.

TTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AM260479/3384874‑3384911 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑accggccACTATGCGCGTGTGCGGCAGAACGCGGCC  <  AM260479/3384940‑3384912                                                                     TTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCACTATGCGCGTGTGCGGCAGAACGCGG    >  1:728760/1‑65 TTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCACTATGCGCGTGTGCGGCAGAACGCGG    <  2:728760/65‑1   GGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCACTATGCGCGTGTGCGGCAGAACGCGGCC  >  1:484925/1‑65   GGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCACTATGCGCGTGTGCGGCAGAACGCGGCC  <  2:484925/65‑1                                                                     TTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AM260479/3384874‑3384911 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑accggccACTATGCGCGTGTGCGGCAGAACGCGGCC  <  AM260479/3384940‑3384912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.