New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	= 3384911	31 (1.160)	4 (0.160)	4/200	2.0	12.5%	coding (409/1086 nt)	trmU	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑methyltransferase
	?	AM260479	= 3384940	27 (1.080)					coding (380/1086 nt)	trmU	tRNA (5‑methylaminomethyl‑2‑thiouridylate)‑methyltransferase
Rejected: Frequency below/above cutoff threshold.

GCGTCTTGGGGATCTCGCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTGATCTCGGCATTGCACAGCACGTCGGGGTTGGGCGTGCGC  >  AM260479/3384791‑3385041                                                                                                                         |                                                                                                                                   gcgTCTTGGGGATCTCGCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGc                                                                                                                       <  1:886359/134‑1 (MQ=255)        ggggATCTCGCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGcaca                                                                                                                 <  1:793124/133‑1 (MQ=255)         gggATCTCGCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACAcg                                                                                                               <  2:242018/134‑1 (MQ=255)         gggATCTCGCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACAcg                                                                                                               >  1:599162/1‑134 (MQ=255)                 gCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGATCAGCCGGCGCAGCAAGTAGCTCTCCTCCTCGGTATGGCCGAGCGCCTTGACCAGCTCGCACCGCCCGGCCGCCTTCTGCCGCACACGCGCCTAgc                                                                                                       >  2:886359/1‑133 (MQ=255)                                    cgcgACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGt                                                                                                                            >  2:719535/1‑94 (MQ=255)                                    cgcgACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGt                                                                                                                            <  1:719535/94‑1 (MQ=255)                                    cgcgACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTc                                                                                    >  2:488024/1‑134 (MQ=255)                                     gcgACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTcc                                                                                   >  2:883220/1‑134 (MQ=255)                                            cTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCa                                                                            <  2:522432/134‑1 (MQ=255)                                              gcgcCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGc                                                                          >  2:939648/1‑134 (MQ=255)                                                gcCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGa                                                                        >  1:308790/1‑134 (MQ=255)                                                     gTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGAcat                                                                     <  2:599162/132‑1 (MQ=255)                                                             ggTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTc                                                            >  2:480523/1‑133 (MQ=255)                                                             ggTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCg                                                           <  1:572824/134‑1 (MQ=255)                                                             ggTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCg                                                           <  2:75569/134‑1 (MQ=255)                                                             ggTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCg                                                           <  2:1002771/134‑1 (MQ=255)                                                                   ggAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGaa                                                      >  1:155914/1‑133 (MQ=255)                                                                   ggAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGaa                                                      <  2:155914/133‑1 (MQ=255)                                                                   ggAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAg                                                     >  1:1018662/1‑134 (MQ=255)                                                                             ctGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGAcat                                                                     <  1:269092/108‑1 (MQ=255)                                                                             ctGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGAcat                                                                     >  2:269092/1‑108 (MQ=255)                                                                                gTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTg                                         <  1:488024/133‑1 (MQ=255)                                                                                    ttGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCactatgcgcgtgtgcggcagaacgcgg                                                                                                         >  1:728760‑M1/1‑38 (MQ=255)                                                                                    ttGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCactatgcgcgtgtgcggcagaacgcgg                                                                                                         <  2:728760‑M1/65‑28 (MQ=255)                                                                                    ttGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTGAtctc                                    >  2:732285/1‑134 (MQ=255)                                                                                      ggTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCactatgcgcgtgtgcggcagaacgcggcc                                                                                                       <  2:484925‑M1/65‑30 (MQ=255)                                                                                      ggTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCactatgcgcgtgtgcggcagaacgcggcc                                                                                                       >  1:484925‑M1/1‑36 (MQ=255)                                                                                      ggTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTGAtctc                                    <  2:848385/132‑1 (MQ=255)                                                                                               gAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCa                                                                            <  1:395112/83‑1 (MQ=255)                                                                                               gAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCa                                                                            >  2:395112/1‑83 (MQ=255)                                                                                                      ttGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCcg                                                                                  >  1:391855/1‑70 (MQ=255)                                                                                                      ttGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCcg                                                                                  <  2:391855/70‑1 (MQ=255)                                                                                                      ttGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTGATCTCGGCATTGCACAGCACGTc                  >  2:552710/1‑134 (MQ=255)                                                                                                                      ggccggccGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTGATCTCGGCATTGCACAGCACGTCGGGGTTGGGCGTgcgc  <  2:308790/134‑1 (MQ=255)                                                                                                                         |                                                                                                                                   GCGTCTTGGGGATCTCGCCCAGCGGGAACAGCGTGCGCGACAGCTGCGCCTGGTTCAGCCGGTGCAGGAAGTAGCTCTGGTCCTTGGTATGGTCGAGCGCCTTGAGCAGCTCGAACCGGCCGGCCGCGTTCTGCCGCACACGCGCATAGTGGCCGGTGGCGATGGTTTCCGCGCCCAGCGACATGGCATGGTCGAGGAAGGCCTTGAACTTGATCTCGGCATTGCACAGCACGTCGGGGTTGGGCGTGCGC  >  AM260479/3384791‑3385041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.