New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260480	= 540111	42 (1.420)	4 (0.160)	4/182	2.0	10.0%	coding (1227/1293 nt)	h16_B0478	7‑Keto‑8‑aminopelargonate synthetase and relatedenzymes
	?	AM260480	= 540095	36 (1.430)					coding (1243/1293 nt)	h16_B0478	7‑Keto‑8‑aminopelargonate synthetase and relatedenzymes
Rejected: Frequency below/above cutoff threshold.

GGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGATTCTTCGGTGTTGC  >  AM260480/539980‑540242                                                                                                                                    |                                                                                                                                    ggCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGt                                                                                                                                   <  2:107053/134‑1 (MQ=255)   cGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTc                                                                                                                                  >  1:256397/1‑133 (MQ=255)   cGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTc                                                                                                                                  >  1:258546/1‑133 (MQ=255)           gCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCAt                                                                                                                         >  1:361487/1‑134 (MQ=255)                           cGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACtt                                                                                                         <  2:256397/134‑1 (MQ=255)                             tCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTtcg                                                                                                       <  1:949319/134‑1 (MQ=255)                                        cgcTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCt                                                                                            <  1:533705/134‑1 (MQ=255)                                            aCCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGc                                                                                         >  2:674020/1‑133 (MQ=255)                                             ccTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcga                                                                                         <  2:114952‑M1/132‑45 (MQ=255)                                             ccTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCg                                                                                        <  2:673770/133‑1 (MQ=255)                                                  gCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACg                                                                                  >  1:388364/1‑134 (MQ=255)                                                           tcCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                                         >  1:821458/1‑134 (MQ=255)                                                            ccAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGc                                                                        >  1:639374/1‑134 (MQ=255)                                                             cAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTgg                                                                                          <  1:310406/115‑1 (MQ=255)                                                             cAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTgg                                                                                          >  2:310406/1‑115 (MQ=255)                                                             cAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTAATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                                         <  2:617745/132‑1 (MQ=255)                                                                       cctcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaagg                                                             >  1:424067‑M1/1‑62 (MQ=255)                                                                         tcGCTGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  2:517944/1‑134 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaagg                                                             >  2:73128‑M1/1‑60 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGAc                                                                             <  2:989958/116‑1 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGAc                                                                             >  1:989958/1‑116 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                                         >  2:218502/1‑120 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                                         <  1:218502/120‑1 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  2:213232/1‑134 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  1:362427/1‑134 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  2:484004/1‑134 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  2:1040416/1‑134 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  1:663253/1‑134 (MQ=255)                                                                         tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                                           >  1:369694/1‑134 (MQ=255)                                                                          cgcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTg                                                          >  2:817963/1‑134 (MQ=255)                                                                          cgcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTg                                                          >  2:308581/1‑134 (MQ=255)                                                                           gcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTgg                                                         <  2:369694/134‑1 (MQ=255)                                                                           gcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTgg                                                         <  2:388364/134‑1 (MQ=255)                                                                                 gCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaaggtagcgcaagc                                                   <  2:424067‑M1/134‑83 (MQ=255)                                                                                            aCCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGc                                        <  1:674020/134‑1 (MQ=255)                                                                                                           tttGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCAt                          >  2:819000/1‑133 (MQ=255)                                                                                                           tttGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCAt                          >  2:340172/1‑133 (MQ=255)                                                                                                            ttGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATc                         <  1:819000/133‑1 (MQ=255)                                                                                                               agaCTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCg                                             >  1:271127/1‑110 (MQ=255)                                                                                                               agaCTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCg                                             <  2:271127/110‑1 (MQ=255)                                                                                                                         cGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGAttctt           >  1:91951/1‑134 (MQ=255)                                                                                                                          gggCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGAttcttc          >  1:994162/1‑134 (MQ=255)                                                                                                                           ggCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaaggtagcgcaagcggaagacggccgcacctcacttgccggcgtcgcccgcgatgc         >  2:1008641‑M1/1‑10 (MQ=255)                                                                                                                           ggCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaaggtagcgcaagcggaagacggcc                                        >  1:300385‑M1/1‑10 (MQ=255)                                                                                                                           ggCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaaggtagcgcaagc                                                   >  2:339118‑M1/1‑10 (MQ=255)                                                                                                                             cccGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaaggtagcgcaagcggaagacggccgcacctcacttgccggcgtcgcccgcgatgcgt       <  1:667544‑M1/134‑127 (MQ=255)                                                                                                                              ccGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGATTCTTCGgtg      <  2:362427/134‑1 (MQ=255)                                                                                                                                ggCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGATTCTTCGGTGtt    <  1:517944/134‑1 (MQ=255)                                                                                                                                  cgcgTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGATTCTTCGGTGTTGc  <  2:639374/134‑1 (MQ=255)                                                                                                                                  cgcgTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGATTCTTCGGTGTTGc  <  2:994162/134‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    GGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATCAATGCCCGATTCTTCGGTGTTGC  >  AM260480/539980‑540242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.