New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260480	= 540111	42 (1.420)	4 (0.160)	4/182	2.0	10.0%	coding (1227/1293 nt)	h16_B0478	7‑Keto‑8‑aminopelargonate synthetase and relatedenzymes
	?	AM260480	= 540095	36 (1.430)					coding (1243/1293 nt)	h16_B0478	7‑Keto‑8‑aminopelargonate synthetase and relatedenzymes
Rejected: Frequency below/above cutoff threshold.

CCACGCATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATC  >  AM260480/539968‑540219                                                                                                                                |                                                                                                                             ccACGCATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCG                                                                                                                        >  1:1008641‑M1/1‑134 (MQ=255)   aCGCATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccggg                                                                                                                      <  1:667544‑M2/134‑9 (MQ=255)     gCATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcc                                                                                                                    >  2:1008641‑M2/1‑124 (MQ=255)      cATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCC                                                                                                                   <  2:396809‑M1/134‑1 (MQ=255)      cATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCC                                                                                                                   <  2:646317‑M1/134‑1 (MQ=255)             ggCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGt                                                                                                            <  2:107053/134‑1 (MQ=255)               cGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTc                                                                                                           >  1:256397/1‑133 (MQ=255)               cGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTc                                                                                                           >  1:258546/1‑133 (MQ=255)                       gCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCAt                                                                                                  >  1:361487/1‑134 (MQ=255)                                    ggCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcc                                                                                                                    >  1:300385‑M2/1‑93 (MQ=255)                                    ggCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCC                                                                                                                    >  2:300385‑M1/1‑103 (MQ=255)                                       cGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACtt                                                                                  <  2:256397/134‑1 (MQ=255)                                         tCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTtcg                                                                                <  1:949319/134‑1 (MQ=255)                                               gCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcccggcgcaagtctcaaaatcccacaagcgggtcgctggcaggc                                                                          <  2:424067‑M2/134‑53 (MQ=255)                                               gCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcc                                                                                                                    >  2:339118‑M2/1‑82 (MQ=255)                                               gCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCC                                                                                                                    >  1:339118‑M1/1‑92 (MQ=255)                                                    cgcTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCt                                                                     <  1:533705/134‑1 (MQ=255)                                                        aCCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGc                                                                  >  2:674020/1‑133 (MQ=255)                                                         ccTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcccggcgcaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgagg                                                                >  1:424067‑M2/1‑72 (MQ=255)                                                         ccTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcccggcgcaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcga                                                                  >  2:73128‑M2/1‑72 (MQ=255)                                                         ccTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcga                                                                  <  2:114952‑M1/132‑45 (MQ=255)                                                         ccTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCg                                                                 <  2:673770/133‑1 (MQ=255)                                                              gCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACg                                                           >  1:388364/1‑134 (MQ=255)                                                                       tcCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                  >  1:821458/1‑134 (MQ=255)                                                                        ccAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGc                                                 >  1:639374/1‑134 (MQ=255)                                                                         cAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTgg                                                                   <  1:310406/115‑1 (MQ=255)                                                                         cAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTgg                                                                   >  2:310406/1‑115 (MQ=255)                                                                         cAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTAATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                  <  2:617745/132‑1 (MQ=255)                                                                                   cctcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaagg                                      >  1:424067‑M1/1‑62 (MQ=255)                                                                                     tcGCTGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  2:517944/1‑134 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTgcgccgggcccggcgcaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaagg                                      <  2:114952‑M2/132‑89 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaagg                                      >  2:73128‑M1/1‑60 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGAc                                                      <  2:989958/116‑1 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGAc                                                      >  1:989958/1‑116 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                  <  1:218502/120‑1 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAgg                                                  >  2:218502/1‑120 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  2:213232/1‑134 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  2:1040416/1‑134 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  2:484004/1‑134 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  1:663253/1‑134 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  1:369694/1‑134 (MQ=255)                                                                                     tcGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCAtt                                    >  1:362427/1‑134 (MQ=255)                                                                                      cgcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTg                                   >  2:308581/1‑134 (MQ=255)                                                                                      cgcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTg                                   >  2:817963/1‑134 (MQ=255)                                                                                       gcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTgg                                  <  2:369694/134‑1 (MQ=255)                                                                                       gcgGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTgg                                  <  2:388364/134‑1 (MQ=255)                                                                                             gCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCaagtctcaaaatcccacaagcgggtcgctggcaggctcccgcgaggagtagtcctggagatggatgcaaaggtagcgcaagc                            <  2:424067‑M1/134‑83 (MQ=255)                                                                                                        aCCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGc                 <  1:674020/134‑1 (MQ=255)                                                                                                                       tttGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCAt   >  2:340172/1‑133 (MQ=255)                                                                                                                       tttGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCAt   >  2:819000/1‑133 (MQ=255)                                                                                                                        ttGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATc  <  1:819000/133‑1 (MQ=255)                                                                                                                           agaCTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCg                      >  1:271127/1‑110 (MQ=255)                                                                                                                           agaCTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCg                      <  2:271127/110‑1 (MQ=255)                                                                                                                                |                                                                                                                             CCACGCATCGCGGGCGACGCCGGCAAGTGAGGTGCGGCCGTCTTCCGCTTGCGCTACCTTTGCATCCATCTCCAGGACTACTCCTCGCGGGAGCCTGCCAGCGACCCGCTTGTGGGATTTTGAGACTTGCGCCGGGCCCGGCGCGTCGAGGACCATTCATCCAGCCGGACTTCGTCGCGGATGCTGGCGAGCACGCCGACCAGGCGGTCGATTTCATTGGCGGCAAGTTTCGCATGCAGCGTAAGTCGCATC  >  AM260480/539968‑540219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.