New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	332818 =	28 (1.110)	6 (0.250)	5/190	1.6	20.7%	coding (1126/1710 nt)	nagE	Protein‑N(pi)‑phosphohistidine‑sugar phosphotransferase II ABC, N‑acetylglucosamine‑specific
	?	AM260479	332850 =	20 (0.850)					coding (1158/1710 nt)	nagE	Protein‑N(pi)‑phosphohistidine‑sugar phosphotransferase II ABC, N‑acetylglucosamine‑specific

GCTGGGGCTGGCCTATGCGCTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGACATCGGCGCGGTGTCCGAGCCGCGGCTCAAG  >  AM260479/332718‑332979                                                                                                                                     |                                                                                                                                  gtcggccacgttcagccgcagacgcgtggtgcaggcatcgaccacgaccaggttggcagggccgcccagcgcttcgacatactgttgcgccacgctgcccgcggccggctgcgcggcaccgccggcggccgcgg                                                                                                                                  >  1:610449‑M2/133‑134 (MQ=255) gCTGGGGCTGGCCTATGCGCTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCgg                                                                                                                                  <  2:722220/134‑1 (MQ=255)    ggccacgttcagccgcagacgcgtggtgcaggcatcgaccacgaccaggttggcagggccgcccagcgcttcgacatactgttgcgccacgctgcccgcggccggctgcgcggcaccgccggcggccgcGGGc                                                                                                                                <  2:610449‑M2/4‑1 (MQ=255)              tATGCGCTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGgcgc                                                                                                                      >  1:353352/1‑133 (MQ=255)                   gcTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAg                                                                                                                 >  1:511931/1‑133 (MQ=255)                    cTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGgc                                                                                                                        <  2:622253/125‑1 (MQ=255)                    cTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGgc                                                                                                                        >  1:622253/1‑125 (MQ=255)                           aTTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGc                                                                                                                                >  2:838869/1‑110 (MQ=255)                           aTTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGc                                                                                                                                <  1:838869/110‑1 (MQ=255)                           aTTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCg                                                                                                          >  1:846544/1‑132 (MQ=255)                                      ttCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGggcgg                                                                                             >  1:863247/1‑134 (MQ=255)                                          gCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCt                                                                                         >  2:539919/1‑134 (MQ=255)                                                   tCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCtg                                                                                <  2:846544/134‑1 (MQ=255)                                                        cgcTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCtggtcgt                                                                           <  2:353352/134‑1 (MQ=255)                                                              aaCCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGa                                                                     <  2:863247/134‑1 (MQ=255)                                                                            gggCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCAcgc                                                       <  1:539919/134‑1 (MQ=255)                                                                             ggCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCtggtcgt                                                                           <  1:1033413/113‑1 (MQ=255)                                                                             ggCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCtggtcgt                                                                           >  2:1033413/1‑113 (MQ=255)                                                                                     tGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGc                                              >  1:286293/1‑134 (MQ=255)                                                                                                 gcccGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGcc                                   <  2:610449‑M1/129‑1 (MQ=255)                                                                                                   tGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGa                                 >  2:168191/1‑133 (MQ=255)                                                                                                   ccGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGAc                                >  1:610449‑M1/3‑134 (MQ=255)                                                                                                      cggccggctgcgcggcaccgccggcggccgcGGGCAGCGTGGCGCAACAGTAt                                                                                                              >  1:876507‑M2/32‑53 (MQ=255)                                                                                                      cggccggctgcgcggcaccgccggcggccgcGGGCAGCGTGGCGCAACAGTAt                                                                                                              <  2:876507‑M2/22‑1 (MQ=255)                                                                                                       ggccgccggctgcgcggcaccgccggccgcGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGcacca                                                          <  1:653549‑M2/74‑1 (MQ=255)                                                                                                       ggccgccggctgcgcggcaccgccggccgcGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGcacca                                                          >  2:653549‑M2/31‑104 (MQ=255)                                                                                                       GGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGACATCg                            <  1:676213‑M1/134‑1 (MQ=255)                                                                                                        GCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGc                                                                                       >  2:270122‑M1/1‑74 (MQ=255)                                                                                                        GCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGc                                                                                       <  1:270122‑M1/74‑1 (MQ=255)                                                                                                           GCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCtggtcgt                                                                           <  2:973639‑M1/83‑1 (MQ=255)                                                                                                           GCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCtggtcgt                                                                           >  1:973639‑M1/1‑83 (MQ=255)                                                                                                                cggTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTAt                                                                                                              <  2:149375/43‑1 (MQ=255)                                                                                                                cggTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTAt                                                                                                              >  1:149375/1‑43 (MQ=255)                                                                                                                              cggccgcGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGca                                                             >  2:401089‑M2/8‑78 (MQ=255)                                                                                                                              cggccgcGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGca                                                             <  1:401089‑M2/71‑1 (MQ=255)                                                                                                                                gccgcGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGACATCGGCGCGGTGTCCGAGCCGCGGCTCaa   <  2:511931‑M2/129‑1 (MQ=255)                                                                                                                                 ccgcGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGACATCGGCGCGGTGTCCGAGCCGCGGCTCAAg  <  2:676213‑M2/130‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  GCTGGGGCTGGCCTATGCGCTGGTCTATTACGGGCTGTTCCGCTTCTTTATCCGCCGCTTCAACCTGCTGACGCCGGGCCGCGATGAAGTGGTGCCCGTGGCGGCCGCCGGCGGTGCCGCGCAGCCGGCCGCGGGCAGCGTGGCGCAACAGTATGTCGAAGCGCTGGGCGGCCCTGCCAACCTGGTCGTGGTCGATGCCTGCACCACGCGTCTGCGGCTGAACGTGGCCGACATCGGCGCGGTGTCCGAGCCGCGGCTCAAG  >  AM260479/332718‑332979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.