| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | AM260479 | 3,399,144 | Δ1,321 bp | [leuS]–h16_A3140 | [leuS], h16_A3140 | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | AM260479 | 3399144 | 3400464 | 1321 | 22 [0] | [0] 22 | [leuS]–h16_A3140 | [leuS],h16_A3140 |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AM260479 | = 3399143 | NA (NA) | 21 (0.840) | 21/196 | 0.2 | NA | noncoding (2/1313 nt) | ISAE1 | insertion sequence ISAE1 |
| ? | AM260479 | 3400465 = | NA (NA) | intergenic (+32/+53) | h16_A3140/dapB | Transposase ISAE1/dihydrodipicolinate reductase | |||||
TGAGCTTCAAAACCATGCGCACCGTGATCTTCCTGATCGCCGGAAAGCTCGACTTCTCCGCGATCAACCCGCATGCCGCATAACCCACTCATTTTTCAAAAGAGCCAAAGAAGACGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGGGCGGCGCGCACGGCA > AM260479/3400351‑3400596 | caggacaacgtttgcgcggcaactggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTggcgg > 2:652711‑M2/115‑132 (MQ=255) aggacaacgtttgcgcggcaactggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCa < 2:418181‑M2/20‑1 (MQ=255) ggcaactggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGttt > 2:940006‑M2/98‑126 (MQ=255) ggcaactggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGttt < 1:940006‑M2/29‑1 (MQ=255) ctggcctgccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTgcgcgc < 1:652711‑M2/42‑1 (MQ=255) ccggcatccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcggc < 2:204255‑M2/50‑1 (MQ=255) atccgggggaatcgttggaatgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCtt < 2:428474‑M2/55‑1 (MQ=255) tgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcggcg > 1:634319‑M2/60‑110 (MQ=255) tgagagcagcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCgcggcg < 2:634319‑M2/51‑1 (MQ=255) gcgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTc > 1:326751‑M2/52‑134 (MQ=255) cgggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCg < 1:601451‑M2/84‑1 (MQ=255) gggcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCg > 1:714928‑M2/50‑133 (MQ=255) gcgcgccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGaa > 1:120089‑M2/48‑133 (MQ=255) ccgcttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGcc > 1:262558‑M2/43‑134 (MQ=255) ttgcgcggcgcccgccgggcgtgcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTg > 2:65038‑M2/39‑134 (MQ=255) gcgtgcaatgcgaaagaagaCGATTATAACCGTggcgg < 2:1010622‑M2/18‑1 (MQ=255) gcgtgcaatgcgaaagaagaCGATTATAACCGTggcgg > 1:1010622‑M2/21‑38 (MQ=255) gcaatgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACggg > 2:983679‑M2/17‑134 (MQ=255) atgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAg > 1:770367‑M2/14‑102 (MQ=255) atgcgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAg < 2:770367‑M2/89‑1 (MQ=255) cgaaagaagaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGGgcggcg < 2:262558‑M2/123‑1 (MQ=255) gaCGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGGGCGGCGCGCACGGCa < 2:326751‑M2/132‑1 (MQ=255) | TGAGCTTCAAAACCATGCGCACCGTGATCTTCCTGATCGCCGGAAAGCTCGACTTCTCCGCGATCAACCCGCATGCCGCATAACCCACTCATTTTTCAAAAGAGCCAAAGAAGACGATTATAACCGTGGCGGCAGGCCAGTTTCGGCCCTGCGCGCCCCGCGGCGCCTTACTTCAGGCCCAGCACGTCCTGCATGTCGAACAGGCCGTTGGGCTTGTCGGCGAGGAAACGGGCGGCGCGCACGGCA > AM260479/3400351‑3400596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |