New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	3186414 =	34 (1.240)	4 (0.160)	4/196	2.0	12.3%	coding (2382/3327 nt)	h16_A2946	Acetyl‑CoA carboxylase (Biotin carboxylase and carboxyl transferase domains)
	?	AM260479	3186433 =	26 (1.040)					coding (2401/3327 nt)	h16_A2946	Acetyl‑CoA carboxylase (Biotin carboxylase and carboxyl transferase domains)
Rejected: Frequency below/above cutoff threshold.

GCCGCGGCCACCGCGTCGGCCTCATCGTCGACCAGCAGGTCGACCACGCCGTTGTGGTACTGCACCGTGGCCGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AM260479/3186551‑3186414 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccggccatgATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTACC  >  AM260479/3186433‑3186562                                                                                                                                                                                                                                                                              GCCGCGGCCACCGCGTCGGCCTCATCGTCGACCAGCAGGTCGACCACGCCGTTGTGGTACTGCACCGTGGCCGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGC                                                                                                                                        <  2:668488/134‑1     CGGCCACCGCGTCGGCCTCATCGTCGACCAGCAGGTCGACCACGCCGTTGTGGTACTGCACCGTGGCCGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATG                                                                                                                                    >  2:409015/1‑134                                                                  CGTGGCCGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATGATCGAGGGCGGCGGCCTGGGCG                                                                                                              <  1:888523/95‑1                                                                  CGTGGCCGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATGATCGAGGGCGGCGGCCTGGGCG                                                                                                              >  2:888523/1‑95                                                                        CGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATGATCGAGGGCGGCGGCCT                                                                                                                   >  1:722006/1‑84                                                                        CGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATGATCGAGGGCGGCGGCCT                                                                                                                   <  2:722006/84‑1                                                                                                                                       CATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTACC  <  1:494122/134‑1                                                                                                                                                                                                                                                                              GCCGCGGCCACCGCGTCGGCCTCATCGTCGACCAGCAGGTCGACCACGCCGTTGTGGTACTGCACCGTGGCCGGGCCGATATCCTCGGGGCGGAACACGCCCAGGCCGCCGCCCTCGATCATGGCCGGGCCGGCCATG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AM260479/3186551‑3186414 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccggccatgATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTACC  >  AM260479/3186433‑3186562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.