New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	3186414 =	34 (1.240)	4 (0.160)	4/196	2.0	12.3%	coding (2382/3327 nt)	h16_A2946	Acetyl‑CoA carboxylase (Biotin carboxylase and carboxyl transferase domains)
	?	AM260479	3186433 =	26 (1.040)					coding (2401/3327 nt)	h16_A2946	Acetyl‑CoA carboxylase (Biotin carboxylase and carboxyl transferase domains)
Rejected: Frequency below/above cutoff threshold.

GAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTACCTGTC  >  AM260479/3186310‑3186566                                                                                                                            |                                                                                                                                      gAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGggcgg                                                                                                                             >  2:494122/1‑134 (MQ=255)             gtggGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCg                                                                                                                  <  2:355926/133‑1 (MQ=255)                      gCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGcc                                                                                                         <  1:1161452/133‑1 (MQ=255)                               cgcTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGatat                                                                                               <  1:677462/134‑1 (MQ=255)                                gctgctTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATc                                                                                              >  2:511425/1‑134 (MQ=255)                                 ctgctTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcg                                                                                             >  1:1159165/1‑134 (MQ=255)                                       tGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                                        <  1:991868/133‑1 (MQ=255)                                           ggCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCAc                                                                                   <  1:139071/134‑1 (MQ=255)                                            gCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                                                  <  1:511425/134‑1 (MQ=255)                                            gCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                                                  <  1:672845/134‑1 (MQ=255)                                            gCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                                                  <  1:1023894/134‑1 (MQ=255)                                                   cgtggccgggccgatatcctcggggcggaacacgcccaggccgccgccctcgatcatggccgggccggccatgATCGAGGGCGGCGGCCTGGGCg                                                                                                                  >  2:888523‑M2/74‑95 (MQ=255)                                                   cgtggccgggccgatatcctcggggcggaacacgcccaggccgccgccctcgatcatggccgggccggccatgATCGAGGGCGGCGGCCTGGGCg                                                                                                                  <  1:888523‑M2/22‑1 (MQ=255)                                                         cgggccgatatcctcggggcggaacacgcccaggccgccgccctcgatcatggccgggccggccatgATCGAGGGCGGCGGCCt                                                                                                                       <  2:722006‑M2/17‑1 (MQ=255)                                                         cgggccgatatcctcggggcggaacacgcccaggccgccgccctcgatcatggccgggccggccatgATCGAGGGCGGCGGCCt                                                                                                                       >  1:722006‑M2/68‑84 (MQ=255)                                                                  ttGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGt                                                              <  2:1159165/132‑1 (MQ=255)                                                                        ccTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGAcc                                                      >  1:636194/1‑134 (MQ=255)                                                                                   cgcccaggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                                                  >  2:888523‑M1/23‑95 (MQ=255)                                                                                   cgcccaggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                                                  <  1:888523‑M1/73‑1 (MQ=255)                                                                                     aCGCGCAACGCCAATATCGGCATGGCCGGGCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTcgacga                                         >  2:1006604/1‑134 (MQ=255)                                                                                        aggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                                        <  2:722006‑M1/67‑1 (MQ=255)                                                                                        aggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                                        >  1:722006‑M1/18‑84 (MQ=255)                                                                                          cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcc                                                                                          >  2:691056/1‑80 (MQ=255)                                                                                          cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcc                                                                                          <  1:691056/80‑1 (MQ=255)                                                                                          cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCa                                                                             <  1:944952/93‑1 (MQ=255)                                                                                          cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCa                                                                             >  2:944952/1‑93 (MQ=255)                                                                                            aCGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAg                                                                                                    <  2:446825/68‑1 (MQ=255)                                                                                            aCGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAg                                                                                                    >  1:446825/1‑68 (MQ=255)                                                                                                     tCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                                        >  2:712845/1‑71 (MQ=255)                                                                                                     tCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                                        <  1:712845/71‑1 (MQ=255)                                                                                                      cGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGatat                                                                                               >  1:719370/1‑63 (MQ=255)                                                                                                      cGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGatat                                                                                               <  2:719370/63‑1 (MQ=255)                                                                                                         CATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCcg                     >  2:409015‑M1/1‑134 (MQ=255)                                                                                                             GCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGc                 <  2:668488‑M1/134‑1 (MQ=255)                                                                                                                  cccggccATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCcg                     >  2:1142055/1‑125 (MQ=255)                                                                                                                  cccggccATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCcg                     <  1:1142055/125‑1 (MQ=255)                                                                                                                        catgATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTAcc      <  1:494122‑M2/130‑1 (MQ=255)                                                                                                                            aTCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTACCTGTc  >  1:1029442/1‑134 (MQ=255)                                                                                                                            |                                                                                                                                      GAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCGCGGCCAGGCATTACCTGTC  >  AM260479/3186310‑3186566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.