New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	3186414 =	34 (1.240)	4 (0.160)	4/196	2.0	12.3%	coding (2382/3327 nt)	h16_A2946	Acetyl‑CoA carboxylase (Biotin carboxylase and carboxyl transferase domains)
	?	AM260479	3186433 =	26 (1.040)					coding (2401/3327 nt)	h16_A2946	Acetyl‑CoA carboxylase (Biotin carboxylase and carboxyl transferase domains)
Rejected: Frequency below/above cutoff threshold.

GTTCGCCGCCTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCG  >  AM260479/3186282‑3186547                                                                                                                                     |                                                                                                                                      gttcgCCGCCTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCa                                                                                                                                      <  2:857562/134‑1 (MQ=255) gttcgCCGCCTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCa                                                                                                                                      <  1:771738/134‑1 (MQ=255)   ggtaatgcctggccgcggccaccgcgtcggcctcatcgtcgaccagcaggtcgaccacgccgttgtggtactgcaccgtggccgggccgatatcctcggggcggaacacgcccaggccgccgccctcgatCATg                                                                                                                                    <  1:494122‑M1/4‑1 (MQ=255)    cgccgcCTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATgg                                                                                                                                   >  2:991868/1‑134 (MQ=255)    cgccgcCTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATgg                                                                                                                                   >  2:677462/1‑134 (MQ=255)          cTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATggccggcc                                                                                                                             >  1:409015/1‑134 (MQ=255)                cGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCa                                                                                                                       >  2:139071/1‑134 (MQ=255)                             gAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGggcgg                                                                                                          >  2:494122/1‑134 (MQ=255)                                         gtggGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCg                                                                                               <  2:355926/133‑1 (MQ=255)                                                  gCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGcc                                                                                      <  1:1161452/133‑1 (MQ=255)                                                           cgcTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGatat                                                                            <  1:677462/134‑1 (MQ=255)                                                            gctgctTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATc                                                                           >  2:511425/1‑134 (MQ=255)                                                             ctgctTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcg                                                                          >  1:1159165/1‑134 (MQ=255)                                                                 tttGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGccggcccg                                                                                                                           <  1:856905/81‑1 (MQ=255)                                                                 tttGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGccggcccg                                                                                                                           >  2:856905/1‑81 (MQ=255)                                                                   tGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                     <  1:991868/133‑1 (MQ=255)                                                                    gCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATggc                                                                                                                                  >  1:780825/1‑71 (MQ=255)                                                                    gCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATggc                                                                                                                                  <  2:780825/71‑1 (MQ=255)                                                                       ggCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCAc                                                                <  1:139071/134‑1 (MQ=255)                                                                        gCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                               <  1:1023894/134‑1 (MQ=255)                                                                        gCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                               <  1:672845/134‑1 (MQ=255)                                                                        gCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                               <  1:511425/134‑1 (MQ=255)                                                                                              ttGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGt                                           <  2:1159165/132‑1 (MQ=255)                                                                                                    ccTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGAcc                                   >  1:636194/1‑134 (MQ=255)                                                                                                               cgcccaggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                               >  2:888523‑M1/23‑95 (MQ=255)                                                                                                               cgcccaggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACg                                                               <  1:888523‑M1/73‑1 (MQ=255)                                                                                                                 aCGCGCAACGCCAATATCGGCATGGCCGGGCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTcgacga                      >  2:1006604/1‑134 (MQ=255)                                                                                                                    aggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                     >  1:722006‑M1/18‑84 (MQ=255)                                                                                                                    aggccgccgccctcgatCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                     <  2:722006‑M1/67‑1 (MQ=255)                                                                                                                      cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcc                                                                       >  2:691056/1‑80 (MQ=255)                                                                                                                      cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcc                                                                       <  1:691056/80‑1 (MQ=255)                                                                                                                      cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCa                                                          <  1:944952/93‑1 (MQ=255)                                                                                                                      cAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCa                                                          >  2:944952/1‑93 (MQ=255)                                                                                                                        aCGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAg                                                                                 >  1:446825/1‑68 (MQ=255)                                                                                                                        aCGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAg                                                                                 <  2:446825/68‑1 (MQ=255)                                                                                                                                 tCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                     >  2:712845/1‑71 (MQ=255)                                                                                                                                 tCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATcggcccg                                                                     <  1:712845/71‑1 (MQ=255)                                                                                                                                  cGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGatat                                                                            <  2:719370/63‑1 (MQ=255)                                                                                                                                  cGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGatat                                                                            >  1:719370/1‑63 (MQ=255)                                                                                                                                     CATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCcg  >  2:409015‑M1/1‑134 (MQ=255)                                                                                                                                     |                                                                                                                                      GTTCGCCGCCTTCGCCGAGCTCAGCGGCGAAGTGCCGGTGGTGGGCATCGCCTCCGGGCGCTGCTTTGCCGGCAATGCCGCGCTGCTGGGCTGTTGCGACCTGATCATCGCCACGCGCAACGCCAATATCGGCATGGCCGGCCCGGCCATGATCGAGGGCGGCGGCCTGGGCGTGTTCCGCCCCGAGGATATCGGCCCGGCCACGGTGCAGTACCACAACGGCGTGGTCGACCTGCTGGTCGACGATGAGGCCGACGCGGTGGCCG  >  AM260479/3186282‑3186547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.