New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	872790 =	28 (1.020)	5 (0.200)	5/196	1.7	17.3%	coding (366/423 nt)	ihfB	integration host factor beta subunit
	?	AM260479	872809 =	22 (0.880)					coding (385/423 nt)	ihfB	integration host factor beta subunit
Rejected: Frequency below/above cutoff threshold.

TTCAAGGCGGGCAAGGAATTGCGCGAACGGGTTGACCGCAGCCAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATTGCTGC  >  AM260479/872662‑872918                                                                                                                                 |                                                                                                                                 ggtgcgcacgcaaggccggcagcaataaaaaaagcgcccctaagggggcgcttttttcaaccacacaagcagcaggcaaaacccggcaatcaggaagagcggacaaaattcagcccaccctcgtgcaaTCccg                                                                                                                              >  1:504876‑M1/129‑133 (MQ=255)          ggCAAGGAATTGCGCGAACGGGTTGACCGCAGCCAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAgcggc                                                                                                                    >  2:953992/1‑134 (MQ=255)             aaGGAATTGCGCGAACGGGTTGACCGCAGCCAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGcggcgg                                                                                                                  >  1:570055/1‑133 (MQ=255)               ggAATTGCGCGAACGGGTTGACCGCAGCCAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAgcg                                                                                                                      >  1:1004448/1‑127 (MQ=255)               ggAATTGCGCGAACGGGTTGACCGCAGCCAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAgcg                                                                                                                      <  2:1004448/127‑1 (MQ=255)                                           cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                    <  1:1054944/133‑1 (MQ=255)                                           cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                    >  2:579127/1‑133 (MQ=255)                                           cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                    >  2:1054944/1‑133 (MQ=255)                                           cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                    <  1:579127/133‑1 (MQ=255)                                             gccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCggg                                                                                                                 >  1:1098320/1‑102 (MQ=255)                                             gccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCggg                                                                                                                 <  2:1098320/102‑1 (MQ=255)                                                 gcgcCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCTGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTc                                                                             >  1:530662/1‑134 (MQ=255)                                                    ttttttcaaccacacaagcagcaggcaaaacccggcaatcaggaagagcggacaaaattcagcccaccctcgtgcaaTCCCGCCGCAGCGGCggg                                                                                                                 >  2:505345‑M1/78‑95 (MQ=255)                                                    ttttttcaaccacacaagcagcaggcaaaacccggcaatcaggaagagcggacaaaattcagcccaccctcgtgcaaTCCCGCCGCAGCGGCggg                                                                                                                 <  1:505345‑M1/18‑1 (MQ=255)                                                      tCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGAt                                                                        >  2:871322/1‑134 (MQ=255)                                                          ggCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGcc                                                                    >  2:620058/1‑134 (MQ=255)                                                                  tGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTg                                                            <  2:570055/134‑1 (MQ=255)                                                                           tCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCTGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTgctgct                                                   <  2:530662/134‑1 (MQ=255)                                                                                   ccggcaatcaggaagagcggacaaaattcagccaaccctcgtgcaaTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGt                                           <  2:936386‑M1/88‑1 (MQ=255)                                                                                        aCGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaa                                      <  1:620058/134‑1 (MQ=255)                                                                                          ggCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaaa                                    <  1:871322/134‑1 (MQ=255)                                                                                                ggCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGcc                              >  2:783260/1‑134 (MQ=255)                                                                                                agagcggacaaaattcagcccaccctcgtgcaaTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTgctgct                                                   <  1:704498‑M1/80‑1 (MQ=255)                                                                                                agagcggacaaaattcagcccaccctcgtgcaaTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTgctgct                                                   >  2:704498‑M1/34‑113 (MQ=255)                                                                                                        cGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAgg                      >  2:100270/1‑134 (MQ=255)                                                                                                          ggCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGt                                                                                                    <  2:736906/54‑1 (MQ=255)                                                                                                          ggCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGt                                                                                                    >  1:736906/1‑54 (MQ=255)                                                                                                          ggCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAgggg                    >  2:738303/1‑134 (MQ=255)                                                                                                               ggTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCtt               >  2:148375/1‑134 (MQ=255)                                                                                                                   ggCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCttttt            <  1:953992/133‑1 (MQ=255)                                                                                                                       aGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTAtt       <  2:972238/134‑1 (MQ=255)                                                                                                                            aGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATtgctgc  <  1:783260/134‑1 (MQ=255)                                                                                                                             gcgcTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaa                                     <  2:81028/98‑1 (MQ=255)                                                                                                                             gcgcTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaa                                     >  1:81028/1‑98 (MQ=255)                                                                                                                                 |                                                                                                                                 TTCAAGGCGGGCAAGGAATTGCGCGAACGGGTTGACCGCAGCCAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATTGCTGC  >  AM260479/872662‑872918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.